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   子宫颈癌细胞系 的翻译结果: 查询用时:0.021秒
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子宫颈癌细胞系
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  cervical carcinoma cell line
     Different plasmid DNAs were transfected into a cervical carcinoma cell line C33A and the influences on P97 activity were evaluated by measuring expressions of luciferase.
     将各种质粒转染到人类子宫颈癌细胞系C33A中 ,检测在不同LCR序列的控制下对病毒启动子P97的活性的影响。
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     Objective To investigate the regulation on signal transduction pathway of gap junction gene connexin(Cx) 43 in human cervical carcinoma cell line HeLa by all trans retinoic acid(ATRA).
     目的 探讨细胞分化诱导剂维甲酸对人子宫颈癌细胞系HeLa中缝隙连接蛋白Cx43信号转导途径的调控作用。
短句来源
     Objectives:To investigate the expression of connexin gene (cx43)in human cervical carcinoma cell line HeLa, and the regulation effect of all trans retinoic acid (ATRA).
     目的 :探讨分化诱导剂维甲酸对肿瘤抑制基因———细胞间隙连接蛋白基因cx43在人子宫颈癌细胞系HeLa中表达的调节作用。
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     AIM To investage the expression of gap junction protein connexin (Cx) 43 in human cervical carcinoma cell line HeLa and its effect on gap junctional intercellular communication.
     目的 探讨细胞间隙连接蛋白 Cx43在人子宫颈癌细胞系 He L a中表达 ,及其对细胞间隙连接通讯的影响 .
短句来源
     Objective To investigate the expression and mechanism of antionco gene connexin (Cx) 43 mRNA and its protein in human cervical carcinoma cell line HeLa.
     目的:探讨新一代肿瘤抑制基因-间隙连接基因Cx43及其蛋白产物在人子宫颈癌细胞系HeLa中的表达及意义。
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  “子宫颈癌细胞系”译为未确定词的双语例句
     Objective: To investigate the programmed cell death of human uterin cervical cancer induced by arsenic trioxide (As\-2O\-3) and research the arsenic trioxide significance of clinical application.
     目的:研究三氧化二砷(As2O3)诱导子宫颈癌细胞系HeLa细胞凋亡,探讨As2O3 对子宫颈癌的临床应用意义。
短句来源
     Phosphorylation of gap junction gene connexin 43 protein and dissociated calcium in HeLa cell line by all-trans -retinoic acid
     维甲酸对人子宫颈癌细胞系HeLa细胞间隙连接蛋白转导途径调控作用的研究
短句来源
     Objective To study the programmed cell death of human uterine cervical cancer induced by arsenic trioxide and investigate the arsenic trioxide significance of clinical application.
     目的:通过研究三氧化二砷(As_2O_3)诱导子宫颈癌细胞系HeLa细胞凋亡作用,探讨As_2O_3在子宫颈癌治疗中的临床意义。
短句来源
     Methods: In situ hybridization(ISH)was employed to detect Cx43 mRNA in HeLa and Cx43 protein was examined by flowcytometer (FCM). Normal liver cell line QZG was used as control.
     方法:应用核酸分子原位杂交和流式细胞仪分析技术,研究人子宫颈癌细胞系HeLa及阳性对照细胞人正常肝细胞系QZG中,间隙连接基因Cx43mRNA及其蛋白产物的表达规律。
短句来源
     Methods The Hela cells were divided into three groups and placed at 37℃, 40℃and 43℃ respectively for 1 hour. Then, the expression of bcl 2, bax and p53 was observed by immunohistochemistry. The average optic density of reaction products was tested by image analysis.
     方法人子宫颈癌细胞系(HeLa细胞)分为37℃、40℃、43℃三个温度组,每组经1h相应的温度处理后,以免疫组织化学的方法检测bcl2、bax和p53的表达并经图象分析仪检测反应产物的光密度并进行统计分析。
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  相似匹配句对
     ESTABLISHMENT OF A HUMAN WELL DIFFERENTIATED CERVICAL CARCINOMA CELL LINE HCC 94 AND ITS CHARACTERIZATION
     人子宫颈癌细胞HCC-94的建立及其生物学特性
短句来源
     Establishment of a human cervical carcinoma cell line HCC-0214 and its biological characteristics
     人子宫颈鳞状细胞癌细胞HCC-0214的建立及其生物学特性
短句来源
     The Recognition of the Cervical Smear Based on Artificial Neural Network
     基于人工神经网络的子宫颈癌细胞识别
短句来源
     Phosphorylation of gap junction gene connexin 43 protein and dissociated calcium in HeLa cell line by all-trans -retinoic acid
     维甲酸对人子宫颈癌细胞HeLa细胞间隙连接蛋白转导途径调控作用的研究
短句来源
     (2)Heterogeneity does exist in gastric adenocarcinoma cell line.
     (2)胃腺癌细胞具有肿瘤异质性;
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  cervical carcinoma cell line
This article discusses the characteristics of the drug-resistant human cervical carcinoma cell line HeLa/MMC and investigates reversal effect of SDZ-PSC833(PSC833) and Verapamil (Ver) upon HeLa/MMC.
      
Objective: To investigate the regulatory effect of curcumin on proliferation and apoptosis in human cervical carcinoma cell line HeLa in vitro.
      
Methods: Human cervical carcinoma cell line Hela was cultured in vitro.
      
Cells from the ME-180 cervical carcinoma cell line were used as target cells.
      
The cervical carcinoma cell line, CaSki, and the breast carcinoma line, MCF-7, responded to the combinations in a manner best described as additive.
      
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In order to establish a cell line of cervical carcinoma in vitro to provide material for further experimental study of this type of tumor,a human well differentiated cervical squmous carcinoma cell derived from a xenograft of nude mice was cultured in vitro.A series of characteristics of the cells was examined according to the rules of establishing a cell line in vitro after the primary cells were successively passaged.The established cell line was cloned by single cell detaching flask.Non serum culture of...

In order to establish a cell line of cervical carcinoma in vitro to provide material for further experimental study of this type of tumor,a human well differentiated cervical squmous carcinoma cell derived from a xenograft of nude mice was cultured in vitro.A series of characteristics of the cells was examined according to the rules of establishing a cell line in vitro after the primary cells were successively passaged.The established cell line was cloned by single cell detaching flask.Non serum culture of the cells was tried.The sensitivity tests to platinum based anticancer drugs were performed in vitro and in vivo.The cells designated as HCC 94 have been so far maintained for 20 months through 151 passages,growing as pavement like arrangement with a population doubling time of 37.46 h and having a tendency to pile up without contact inhibition.The presence of typical desmosomes between the cells and many tonofilaments in cell chromosome analysis revealed the number of desmosomes per cell varied from 2 to 126 with a stem line number of 71 100(59.0%).The pseudodiploid population of the cells accounted for 50%.The morphology of chromosomes showed human tumor cell structure.The tumor markers(ER,PR,CEA,PcNA)and the oncogenes(p 53 、nm 23 、cerbB 2)of the cells had a higher expression and amplification in comparision with the original tumor,detected by immunocytochemical technique.The HPV16,18 of the cells using PCR were negative.The index of DNA using FCM(flow cytometry)was 1.55.The cells were highly tumorigenic in nude mice and the transplanted tumors were well differentiated squamous carcinoma which closely resembled the original tumor.HPV16 of the grafts recurred.Three sub cell strains were cloned from the HCC 94.The cell line was also successfully cultured in non serum medium.The drugs inhibited proliferation of the cells in vitro and in vivo.The results showed that the cell line established may be useful in further studying in vitro and in vivo the properties of human cervical carcinoma.

为建立子宫颈癌的体外培养细胞系,以人子宫颈高分化鳞癌裸鼠移植瘤为材料进行体外培养,原代培养生长后行传代培养,并按照细胞系建立标准检测细胞的一系列生物学特性,并行克隆、无血清培养及对铂类抗癌药物的敏感性试验。结果:细胞维持培养20个月,传代151代,生长稳定,群体倍增时间为37.46h,细胞呈上皮镶嵌状贴壁生长,趋向复层生长,无接触抑制。超微结构显示具有典型的桥粒结构和较多的张力原纤维。染色体数目2~126,假2倍体占50%,主流范围71~100条(59%),结构为人类染色体。细胞的肿瘤标记物(ER、PR、CEA、PcNA)及癌基因蛋白产物(p53、nm23、CerbB-2)免疫细胞化学检测呈高表达,PCR检测HPV16、18均阴性,FCM检测DNA指数为1.55。裸鼠移植瘤组织病理形态学与患者原始肿瘤一致,且HPV16阳性。细胞系已克隆出3个亚株,无血清培养成功,体外、体内对铂类抗癌药物均敏感。表明该细胞系符合建系标准,是一株新的子宫颈癌细胞系,为子宫颈癌的实验研究提供了理想的材料

Objectives:To investigate the expression of connexin gene (cx43)in human cervical carcinoma cell line HeLa, and the regulation effect of all trans retinoic acid (ATRA).Methods:In situ hybridization (ISH),flowcytometer (FCM),Western blot and Lucifer Yellow scrape loading and dye transfer (SLDT) were used to detect the expression of cx43 mRNA and protein in HeLa cell,and the regulation effect of ATRA. Results:After treatment with ATRA,HeLa cell grew much slowly than untreated cell.ISH showed cx43 mRNA detectable...

Objectives:To investigate the expression of connexin gene (cx43)in human cervical carcinoma cell line HeLa, and the regulation effect of all trans retinoic acid (ATRA).Methods:In situ hybridization (ISH),flowcytometer (FCM),Western blot and Lucifer Yellow scrape loading and dye transfer (SLDT) were used to detect the expression of cx43 mRNA and protein in HeLa cell,and the regulation effect of ATRA. Results:After treatment with ATRA,HeLa cell grew much slowly than untreated cell.ISH showed cx43 mRNA detectable and up regulation.FCM and Western blot found the positive rate of cx43 in 43KDa protein in HeLa cells increasing from 1.9%to 26.3%.Gap junction intracellular communication (GJIC) recovered in RA treated cell was more than untreated cell. Conclusion:The anti tumor effect of ATRA on HeLa Cell might be due to up regulation of cx43 gene and recovery of cx mediated GJIC.

目的 :探讨分化诱导剂维甲酸对肿瘤抑制基因———细胞间隙连接蛋白基因cx43在人子宫颈癌细胞系HeLa中表达的调节作用。方法 :应用核酸原位杂交、流式细胞仪、Westenblot及LuciferYellow划痕标记荧光传输技术 ,研究维甲酸作用对HeLa细胞cx43mRNA及其蛋白表达 ,以及对HeLa细胞生长和通讯功能的调节作用。结果 :HeLa细胞经维甲酸处理后 ,原位杂交显示 ,HeLa细胞cx43mRNA水平上调 ;流式细胞仪分析 ,Cx43蛋白荧光强度增强 ,阳性细胞计数率由 1.9%上升至 2 6 .3 % ;Westerblot检测到分子量约 43KDa的Cx43蛋白表达 ;HeLa细胞生长明显受抑 ;细胞间隙连接通讯功能有所恢复。结论 :维甲酸可通过上调肿瘤抑制基因cx43及其蛋白在HeLa细胞中的表达 ,实现对宫颈癌细胞恶性表型的逆转作用 ,这可能是维甲酸肿瘤抑制作用的重要机制之一。

Objective To investigate the expression and mechanism of antionco gene connexin (Cx) 43 mRNA and its protein in human cervical carcinoma cell line HeLa.Methods: In situ hybridization(ISH)was employed to detect Cx43 mRNA in HeLa and Cx43 protein was examined by flowcytometer (FCM). Normal liver cell line QZG was used as control. Results: In HeLa cell line, ISH showed that Cx43 mRNA was negative, whereas normal liver cell line QZG was positive. In HeLa and QZG,the positive rates of Cx43 protein were 1. 9% and...

Objective To investigate the expression and mechanism of antionco gene connexin (Cx) 43 mRNA and its protein in human cervical carcinoma cell line HeLa.Methods: In situ hybridization(ISH)was employed to detect Cx43 mRNA in HeLa and Cx43 protein was examined by flowcytometer (FCM). Normal liver cell line QZG was used as control. Results: In HeLa cell line, ISH showed that Cx43 mRNA was negative, whereas normal liver cell line QZG was positive. In HeLa and QZG,the positive rates of Cx43 protein were 1. 9% and 99.0% respectively (P<0. 01). Conclusions: The aberrant location of antioncogene Cx43 mRNA and its protein in human cervical carcinoma cell line HeLa may be responsible for genesis of cervical carcinoma.

目的:探讨新一代肿瘤抑制基因-间隙连接基因Cx43及其蛋白产物在人子宫颈癌细胞系HeLa中的表达及意义。方法:应用核酸分子原位杂交和流式细胞仪分析技术,研究人子宫颈癌细胞系HeLa及阳性对照细胞人正常肝细胞系QZG中,间隙连接基因Cx43mRNA及其蛋白产物的表达规律。结果:原位杂交显示,Cx43mRNA在HeLa细胞中呈阴性,在QZG细胞中呈强阳性。流式细胞仪分析示Cx43蛋白在HeLa、QZG细胞中阳性细胞计数率分别为1.9%和99.0%,差异有高度显著性(P<0.01)。结论:CX43基因及其蛋白产物在HeLa中表达缺失可能与子宫颈癌发生恶性表型相关。

 
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