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蛋白p
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  protein p
     Expression of a synapse-associated membrane protein, P84/SHPS-1 and its ligand IAP/CD47 in mouse retina
     小鼠视网膜一种突触膜结合蛋白P84/SHPS-1和它的配体IAP/CD47的表达(英文)
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     Objective To study the expression of recombinant plasmid pcDNA3.1-P30-P22-CTXA_2/B in Hela cells,and detect the immunological activity of fusion protein P30-P22-CTXA_2/B.Method Hela cells were transfected with recombinant plasmid pcDNA3.1-P30-P22-CTXA_2/B by liposomes.
     目的观察重组质粒 pcDNA3.1-P30-P22-CTXA_2/B 在 Hela 细胞中的表达情况,并检测其表达的融合蛋白 P30-P22-CTXA_2/B 免疫学活性。
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     Epididymal Sperm Protein P34H and Male Reproduction
     附睾精子蛋白P34H与男性生殖
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     Results: The positive rates of Bcl 2 protein, P53 protein, ER, and PR in breast carcinoma tissues were 65.2%, 47.5%,75.4%, and 69.6% respectively.
     结果 :Bcl 2蛋白 ,P53蛋白 ,ER ,PR在乳癌组织中表达的阳性率分别为 65 .2 % ,47.5 % ,75 .4 % ,69.6 % ;
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     Nuclear protein P8 and endocrine tumors
     核内蛋白P8与内分泌肿瘤的研究进展
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  “蛋白p”译为未确定词的双语例句
     The specific activity is 426.2 + 48.4 u/mg pfptein ( x + SD ) : the arrange of the value measured is 217.2-589.1 u/mg prutein and the value of P95 is 915.0 u/mg protein.
     其比活力为426.2±48.4u/mg蛋白(x±SD),测定值范围为217.2~589.1u/mg蛋白,P95>915.0u/mg蛋白。
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     Skp2 expression was negatively correlated with both P27 expression( rs=- 0.451, P<0.001) and PTEN expression ( rs=- 0.480, P<0.001) ingastric carcinoma.
     胃癌Skp2表达与靶蛋白P27表达(rs=-0.451,P=0.000)和肿瘤抑制蛋白PTEN(rs=-0.480,P=0.000)表达呈负相关;
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     Expression and correlativity of HCV core,p53, Mdm2 and p14~(ARF) protein in HCV hepatitis and cirrhosis
     HCV核心蛋白,p53,Mdm2,p14~(ARF)在HCV肝炎肝硬化的表达及相关性
短句来源
     Purpose To study the expression of human papilloma viruses 16 and 18 (HPV16/18) DNA and the cell cycle proteins of p16~ Ink4a and Rb in cervical adenocarcinoma and the influence of HPV16/18 infection on expressions of p16~ Ink4a and Rb protein.
     目的研究16、18型人乳头瘤病毒(HPV16/18)DNA与细胞周期相关蛋白p16Ink4a、Rb在子宫颈腺癌中的表达情况及HPV16/18感染对p16Ink4a、Rb蛋白表达的影响。
短句来源
     Objective:To investigate the expression and significance of the multidrug resistance-related genes multidrug resistance gene1(MDR1)、multidrug resistance-related protein(MRP)、lung resistance protein(LRP)mRNA and their proteins P-glycoprotein(P-gp)、MRP、LRP in human non small cell lung cancer(NSCLC)tissues and paratumor tissues.
     目的:研究探讨非小细胞肺癌(NSCLC)组织和癌旁组织中多药耐药基因1(multidrug resistance gene1,MDR1)、多药耐药相关蛋白(multidrug resistance-related proteins,MRP)、肺耐药蛋白(lung resistance protein,LRP)mRNA及其相应蛋白P-gp、MRP、LRP的表达情况及临床意义。
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  相似匹配句对
     MLL protein;
     MLL蛋白;
短句来源
     proteinuria(-);
     尿蛋白(-);
短句来源
     P53 oncoprotein was localized inthe nuclei.
     P53蛋白
短句来源
     These results indicated that EAPH is a kind of high quality protein product.
     EAPH为优良蛋白制品。
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  protein p
This paper summarizes the results from molecular modeling of various multidrug resistance (MDR) modulators and the MDR protein P-glycoprotein (P-gp).
      
Expression ofmyc broke the regulation of the cell cycle, in particular, canceled the G1 phase arrest for cells with damaged DNA, despite the normal function of protein p53 and induction of the p53-responsive genep21Waf1 in these cells.
      
We studied the relation between genetic anomalies in thep73 gene encoding a product structurally and functionally similar to the protein p53 and the pathogenesis of non-small-cell lung carcinoma (NSCLC; 83 patients).
      
We undertook a search for proteins interacting with protein p45 using the method of two-hybrid screening in order to determine the function of the Sec14p-like protein p45.
      
A screening of the yeast library of rat lung cDNA allowed the identification of six proteins specifically activating the reporter genes of a two-hybrid system and 21 unlikely protein partners of protein p45.
      
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Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV...

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV ) , subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts ( CEF ) . They can be used to differentiate exogenous from endogenous ( RAV-O ) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 ( IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb...

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(27)和pr~(76)(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。

In this study, CaM activity in supernatant of tissues from normal and Sham-operated controls and two kidney-one clip renal hypertensive rats(RHR) were examined with phosphodiesterase (PDE) bioassay as well as cAMP contents in aorta. The results showed that the contents of CaM ia the right kidney of RHR, normal and Sham-operated controls were 38.4±5.5, 18.7±1.74 and 17,3.1.65 ng/ug DNA respectively (P<0.05)iand contents of CaM in aorta were 0.56±0.09, 0.31±0.11 and 0.31± 0.11 and o.32±0.07 μg/mg protein respectively...

In this study, CaM activity in supernatant of tissues from normal and Sham-operated controls and two kidney-one clip renal hypertensive rats(RHR) were examined with phosphodiesterase (PDE) bioassay as well as cAMP contents in aorta. The results showed that the contents of CaM ia the right kidney of RHR, normal and Sham-operated controls were 38.4±5.5, 18.7±1.74 and 17,3.1.65 ng/ug DNA respectively (P<0.05)iand contents of CaM in aorta were 0.56±0.09, 0.31±0.11 and 0.31± 0.11 and o.32±0.07 μg/mg protein respectively (P<0.02). Besides, the cAMP contents in aorta were 7.3±1.18, 2.93±0.75 and 1.58±0.49 pmol/mg protein. There was no difference between normal and Sham-operated controls and the CaM content in heart has also no significant differences. The authors suggest thatCa++ and cAMP play a role in the pathogenesis of hypertension through regulating function of vascular smooth muscle and reain angiotensin-aldosterone system.

在。“两肾一环”肾性高血压大鼠观察到健侧肾脏CaM含量明显升高,与对照组及假手术组比较。分别为38.4±5.5;18.7±1.74和17.3±1.65ng/μgDNA(P<0.05)。实验组主动脉CaM含量也明显增加,与对照组及假手术组比较分别为0.65±0.09,0.31±0.11和0.32±0.07μg/mg蛋白(P<0.02)。实验组主动脉cAMP含量为7.30±1.18,明显高于空白对照组2.97±0.75(pmol/mg蛋白) (P<0.02)及假手术组1.58±0.49 pmol/mg(P<0.001)。而假手术组与对照组之间无明显差异。在心脏组织中各组间CaM含量无明显差异。经初步分析推测Ca及cAMP可能通过参与对血管平滑肌及肾素-血管紧张素-醛固酮系统的调节而对高血压的发病产生作用。

A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known...

A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known anti-P_(30).It has a molecular weight of about 30,000 demonstrated by SDS-PAGE. We conclude that the human semenspecific protein isolated by this method would be named as P_(30) in this report.

本文介绍人精浆特异蛋白P_(30)分离纯化及鉴定的方法。人精浆经蒸馏水透析后经Sephadex G100、G150和G75三种不同的柱层析即可获得精浆特异蛋白。SDS-PAGE、免疫电泳、免疫双扩散证明其纯度和特异性均符合要求。它与已知抗P_(30)血清呈强阳性反应,用其免疫制备的抗血清和已知抗P_(30)血清,在免疫电泳中只与精浆或精浆特异蛋白形成一条完全相同的沉淀线,SDS-PAGE测得分子量约为30,000。故笔者将其亦定名为P_(30)。

 
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