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   家蚕培养细胞 的翻译结果: 查询用时:0.377秒
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家蚕培养细胞
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  silkworm cell
     These results implied that the infectivity of the chemical mutated BmNPV to silkworm cell and larvae had no distinct change.
     研究结果提示 ,诱变的BmNPV对家蚕培养细胞、虫体组织的感染性无显著变化
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  “家蚕培养细胞”译为未确定词的双语例句
     METHODS: A recombinant transfer vector pBacPAK-CE2, containing total cDNA of CE2 gene with 2a HCV strain, was constructed and co-transfected into BmN cells with linearized BmBacPAK (modified BmNPV) DNA for the construction of a recombinant baculovirus carrying CE2 fused gene.
     方法:将2a型HCV全长CE2基因的cDNA重组于质粒pBacPAK8,构建重组转移载体pBacPAK CE2,与经线性化修饰的家蚕核型多角体病毒(BmNPV)DNA共转染家蚕培养细胞株,构建重组病毒.
短句来源
     Expression of HCV CE2 fused protein in cultured cells and larvae of silkworm and initial application
     HCVCE2融合蛋白在家蚕培养细胞、蚕幼虫中的表达及其初步应用
短句来源
     Expression and Identification of Human Hepatitis B Virus Protein PreS1 in Silkworm Bombyx mori Cell
     人乙肝病毒前表面抗原PreS1在家蚕培养细胞中的表达
短句来源
     In this study a VP2 gene was inserted into a Bombyx mori baculovirus transfer vector pBacPAK8 and cotransfected with linear virus Bm BacPAK6 into BmN cells.
     为研究 VP2作为 IBDV亚单位疫苗的潜力 ,本实验将 IBDV JD1株的 VP2基因重组于家蚕杆状病毒转移载体 p Bac PAK8中 ,将重组载体 p Bac PAK-VP2与病毒 Bm-Bac PAK6的线性化 DNA共转染家蚕培养细胞 ,获得重组病毒 Bac PAK-VP2。
短句来源
     Human pro-UK cDNA was inserted into Bombyx mori nuclear polyhedrosis virus (BmNPV) transfer vectors pBK283 and pBF4. The resulting plasmids and the BmNPV genomic DNA were co-transfected into Bm-N cells, and two stable recombinant viruses BmNPV-pk1 and BmNPV-pk2 were isolated by plaque assay on Bm-N cells.
     将人尿激酶原(pro-UK)cDNA分别插入家蚕核型多角体病毒转移载体pBK283和pBF4中,构建成两个重组质粒。 所构建的这两个重组质粒与野生型家蚕核型多角体病毒DNA(BmNPV genomic DNA)共转染家蚕培养细胞,经病毒斑实验(Plaque assay)筛选出含pro-UK cDNA的稳定重组病毒株BmNPV-pk1和BmNPV-pk2。
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  相似匹配句对
     Primary culture studies have shown that exogenous NGF induces differentiation of NB cells depending their survival on NGF.
     一、细胞培养
短句来源
     LOCALIZED CELL CULTURE
     定位细胞培养
     Establishment and Suspension Cultivation of Silkworm Bombyx mori Cell Line BmN ZJ S
     家蚕悬浮培养细胞系的建立及悬浮培养
短句来源
     SILKWORM OVARIAN MONOLAYER CELL CULTURE AND VIRUS INFECTION
     家蚕卵巢单层细胞培养及其病毒感染试验
短句来源
     Expression of Human Interleukin-11 in Cell Culture and Larvae of Silkworm
     人白细胞介素-11基因在家蚕培养细胞和虫体内的表达
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  silkworm cell
In the present study, we have used the φC31 integrase system to mediate site-specific recombination in the cultured silkworm cell line BmN4.
      
The control promoter (hsp70) promoted the expression of chloramphenicol acetyltransferase (CAT) gene ligated at the downstream, dependent on the orientation of the promoter in the silkworm cell.
      
Toward this goal, we have established 11 clones (designated NISES-BoMo-DK1 to 11) from a previously reported silkworm cell line, NISES-BoMo-DZ.
      
Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4
      
By using linearized plasmid substrates, we have detected intramolecular NHEJ activity in a cell-free extract from the cultured silkworm cell line BmN4.
      


Human pro-UK cDNA was inserted into Bombyx mori nuclear polyhedrosis virus (BmNPV) transfer vectors pBK283 and pBF4. The resulting plasmids and the BmNPV genomic DNA were co-transfected into Bm-N cells, and two stable recombinant viruses BmNPV-pk1 and BmNPV-pk2 were isolated by plaque assay on Bm-N cells. When Bm-N cells and silkworm larvae were infected with recombinant viruses BmNPV-pkl and BmNPV-pk2, the samples of cells fluid and silkworm larvae haemolymph were characterized with Western blot analysis, the...

Human pro-UK cDNA was inserted into Bombyx mori nuclear polyhedrosis virus (BmNPV) transfer vectors pBK283 and pBF4. The resulting plasmids and the BmNPV genomic DNA were co-transfected into Bm-N cells, and two stable recombinant viruses BmNPV-pk1 and BmNPV-pk2 were isolated by plaque assay on Bm-N cells. When Bm-N cells and silkworm larvae were infected with recombinant viruses BmNPV-pkl and BmNPV-pk2, the samples of cells fluid and silkworm larvae haemolymph were characterized with Western blot analysis, the blot displayed a major band at 50kDa. Enzymatic acticity in fibrin plate assay was 120IU per mL of cell supernatant. The expression level of pro-uk in the cells fluid 4 days post-infection was 3.1μg/mL, and in infected larvae haemolymph was 30.0μg/mL determined by ELISA.

将人尿激酶原(pro-UK)cDNA分别插入家蚕核型多角体病毒转移载体pBK283和pBF4中,构建成两个重组质粒。所构建的这两个重组质粒与野生型家蚕核型多角体病毒DNA(BmNPV genomic DNA)共转染家蚕培养细胞,经病毒斑实验(Plaque assay)筛选出含pro-UK cDNA的稳定重组病毒株BmNPV-pk1和BmNPV-pk2。将此两株重组病毒分别感染家蚕培养细胞和家蚕幼虫4天后,用酶联免疫测定法(ELISA),纤维蛋白平板溶圈测活法和Western印迹法分析细胞培养上清及细胞和家蚕的体液及组织,证实均有pro-UK表达。家蚕培养细胞的表达量分别为0.0012mg/mL(上清液和10~6细胞)和0.0031mg/mL(上清液和10~6细胞),家蚕幼虫体液的表达量分别为0.0100mg/mL和0.0300mg/mL。从以上结果可看出,家蚕幼虫体液的表达量是培养细胞的10倍,以上表达产物在纤维蛋白平板上测活均有溶纤活性。

More and more foreign genes have been ex-pressed in the silkworm larvae or silkworm celllines using the Bombyx inori nuclear polyhe-drosis virus (BmNPV) as a expression vector.The expressed products involve in many fieldssuch as pharmaceutics. medical diagnosis, vac-cineperoduction and biological control. Thecharacteristics of BmNPV and its genomestructure. characteristics of polyhedrin gene.construction of recombinant BmNPV and itsexpression in the silkworm larvae and cell line,and efficiency of production...

More and more foreign genes have been ex-pressed in the silkworm larvae or silkworm celllines using the Bombyx inori nuclear polyhe-drosis virus (BmNPV) as a expression vector.The expressed products involve in many fieldssuch as pharmaceutics. medical diagnosis, vac-cineperoduction and biological control. Thecharacteristics of BmNPV and its genomestructure. characteristics of polyhedrin gene.construction of recombinant BmNPV and itsexpression in the silkworm larvae and cell line,and efficiency of production for the foreigngene products expressed in the silkworm-Bm-NPV system and application of the expressedproduct were described systematically in thereview.

以家蚕核多角体病毒(BmNPV)为载体,在家蚕幼虫或家蚕培养细胞系中表达的外源基因越来越多,其表达的产物已涉及到医用药物、医疗诊断、疫苗生产、生物防治等诸多领域,文章就BmNPV的特性及其基因组构造,多角体蛋白基因的特性,重组BmNPV的构建及其在家蚕幼虫体内和细胞系中的表达,BmNPV-家蚕表达系统的外源蛋白生产效率及其应用等各个方面作了全面、系统的综述。

The recombinant BmNPV was injected early in the fifth instar stage into the body of silkworm larvae. The contents of HBsAg detected by ELISA were 7 ̄12 μg·mL -1 haemolymph. Particles which is the same as that of HBsAg can be seen under the electron microscope when immuno-precipitated by anti-LHRH antibody.

以在家蚕培养细胞中获得的重组BmNPV病毒感染5龄的家蚕,在感染后第24h可以测出HBsAg的活性。以后随着感染时间的延长,HBsAg的量也逐渐升高。用ELISA抑制法测得每血淋巴中含HBsAg-LHRH的量为7~12μg·mL-1。以兔抗LHRH抗体对家蚕血淋巴进行免疫沉淀,在电子显微镜下观察到与乙肝病毒表面抗原大小类似的颗粒。

 
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