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凋亡率分别
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  apoptotic rates
     After 18 h,the apoptotic rates by combined use of 0.2 μmol/L STI571 and 10 or 30 μmol/L curcumin were (15.44±2.92) % and(28.16±3.22) % respectively.
     STI571 0.2μmol/L与姜黄素10、30μmol/L联合作用18 h后的凋亡率分别为(15.44±2.92)%和(28.16±3.22)%。
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     The apoptotic rates of SW480 cells after treatment with 500 μg/L TRAIL for 0,6,12,18,24 and 30 hours were 7.8 %,21.4 %,41.8 %, 60.6 %,73.8 % and 77.3 %, respectively.
     500μg/LTRAIL蛋白于0,6,12,18,24和30h诱导结肠癌SW480细胞的凋亡率分别为7.8%,21.4%,41.8%,60.6%,73.8%和77.3%.
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     ②At 24h,apoptotic rates of different groups (1.25%,2.5%,5.0%,10.0%) treated with extracts of AF were 0.61%,4.1%, 6.2%, 24.9% respectively,diploid rates were 0%, 10.6%, 34.7%,72.2% respectively.
     1.25%、2.5%、5.0%、10.0%大葱提取液作用24h,凋亡率分别为0.6%、4.1%、6.2%、24.9%,二倍体比例分别为0%、10.6%、34.7%、72.2%。
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     By FCM methods, the apoptotic rates of LoVo cells were 11.32%±0.92%, 19.23%±0.78%, 24.51%±5.88%, 42.22%±2.32%, after treatment of 23-hydroxybetulinic acid at concentrations 25, 50, 100, 200 μmol·L-1, which showed obvious concentration-dependent relationship.
     23-羟基白桦酸25、50、100及200μmol·L-1作用LoVo细胞48h后,其凋亡率分别为11.32%±0.92%、19.23%±0.78%、24.51%±5.88%及42.22%±2.32%,显示一定的剂量依赖性关系。
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     By FCM analysis, the apoptotic rates at concentrations of 0 mg/L, 50 mg/L, 75 mg/L, 100 mg/L, 150 mg/L, 200 mg/L were 2.2 %, 20.3 %, 25.3 %, 29.8 %, 45.1 % and 53.1 %, respectively.
     流式细胞仪定量分析,0,50、75、100、150、200 mg/L浓度下凋亡率分别为2.2%,20.3%,25.3%,29.8%,45.1%和53.1%.
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  “凋亡率分别”译为未确定词的双语例句
     the apoptosls rate was 11.6% and 15.7%,26.2% and 13.4%.
     凋亡率分别为11.6%,15.7%,26.2%,13.4%。
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     The ratio of apoptosis was(4.69±0.60)%,(21.15±3.10)%,(33.15±2.40)%,(40.87±1.50)% and(48.47±1.02)% respectively by FCM.
     流式细胞仪检测显示不同浓度凋亡率分别为(4.69±0.60)%、(21.15±3.10)%、(33.15±2.40)%、(40.87±1.50)%、(48.47±1.02)%;
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     The apoptotic cell rate was 4.36%, 9.87%, 10.80%, 29.65%, 76.80% respectively after SKOV3 cells were treated with VK3 for 48h at concentrations of 0, 2, 5, 10, 20 μmol/L.
     不同浓度VK3(0、2、5、10、20μmol/L)作用48h后,SKOV3细胞的凋亡率分别是4.36%,9.87%,10.80%,29.65%,76.80%。
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     When the concentrations of chitosan were 0.1,1,and 10mg/ml,the apoptosis rate of fibroblasts were 5.83%±1.35%,8.94%±1.67% and 26.23%±4.56%,respectively,which were significantly higher than that of control group(1.53%±0.35%,P<0.05).
     几丁糖浓度为0.1、1、10mg/ml时,成纤维细胞的凋亡率分别为5.83%±1.35%、8.94%±1.67%、26.23%±4.56%,显著高于空白对照组(1.53%±0.35%,P<0.05);
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     The percent of apoptosis was 7.20%±0.43% and 12.73%±0.75% after repeated irradiation with 100mW/cm2 for 10min and 30min respectively.
     100mW/cm2照射细胞10,30min时凋亡率分别为(7.20±0.43)%、(12.73±0.75)%;
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  相似匹配句对
     The apoptosis rate was 24%, 26%and 33%respectively.
     凋亡分别为24%,26%和33%。
短句来源
     and the apoptosis rate of P388 cells induced by VP-16+DXM was 71.9%±9.9%, 66% higher than that induced by only VP-16 (38.1%±3.8%) (all P<0.01).
     凋亡增加分别为 44 %、89%和 6 6 % ;
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     Apoptosis rate shows an increasing trend;
     凋亡呈现增加的趋势;
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     The positive staining of p53 located in the nuleus.
     p53的阳性表达分别
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     Flurorescence staining was used to detect the percentage of apoptosis.
     荧光染色检测凋亡;
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  apoptotic rates
After treatment with 10 μmol/L of Bcl-2 antisense PNA or antisense ODN for 72 h, apoptotic rates of K562 cells were 13.15±1.13 and 11.72±1.12, respectively.
      
Cellular apoptotic rates were determined by using TUNEL.
      
The apoptotic rates and the cell cycles were determined by means of flow cytometry, the apoptosis indexes were detected by using TUNEL, and the expression of caspase-3 was measured by using SP immunohistochemistry.
      
Both models of SBR led to significant increases in enterocyte apoptotic rates compared to respective sham levels; however, apoptotic rates were 2.5-fold higher in ileal compared to jejunal segments (P>amp;lt;0.01).
      
The significant difference in EC apoptotic rates between proximal and distal intestinal segments appeared to be due to utilization of different mechanisms of action.
      
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We investigated the effect of laser irradiation to induce apoptosis of vascular smooth muscle cells(SMC). Rabbit SMC, isolated from the thoracic arota, were cultured in DMEM containing 10% fetal calf serum. SMC from passages 5 and 6 were irradiated by 510 nm copper vapor laser with the power densities of 25, 50, 75, 100, 200 mW/cm 2 and the energy densities of 25, 50, 75, 100, 200 J/cm 2. Apoptosis of SMC were identified by specific labeling of nuclear DNA fragment (TUNEL) at 4, 12, 24, 48 and 72 hours after...

We investigated the effect of laser irradiation to induce apoptosis of vascular smooth muscle cells(SMC). Rabbit SMC, isolated from the thoracic arota, were cultured in DMEM containing 10% fetal calf serum. SMC from passages 5 and 6 were irradiated by 510 nm copper vapor laser with the power densities of 25, 50, 75, 100, 200 mW/cm 2 and the energy densities of 25, 50, 75, 100, 200 J/cm 2. Apoptosis of SMC were identified by specific labeling of nuclear DNA fragment (TUNEL) at 4, 12, 24, 48 and 72 hours after irradiation and the necrotic cells were determined by the trypan blue dye exclusion test. It was found that SMC began to undergo apoptosis at 4 hours after irradiation by 50 and 75 J/cm 2 and the number of apoptotic cells reached a maximum at 24 hours. This result indicated that 510 nm copper vapor laser irradiation may induce apoptosis of SMC and it should attract attention as a possible approach to reduce coronary restenosis.

为了探讨用激光防治血管成形术后再狭窄的可能性,以510nm激光照射体外培养的血管平滑肌细胞,以DNA片断末端标记法观察细胞凋亡现象。结果显示,50和75J/cm2照射后4小时SMC出现凋亡现象,24小时凋亡最为显著,凋亡率分别为9.4%和6.1%。表明激光直接照射能引起平滑肌细胞凋亡。这可能有益于减轻血管内膜的增厚程度

Objective: To investigate the relationship between pulmonary eosinophil (Eos) infiltration and apoptotic dysregulation in asthmatic guinea pigs. Methods: After the model of asthma was established with ovalbumin sensitization in guinea pigs, the dynamic changes of pulmonary Eos infiltration, cytology of braonchoalveolar lavage fluid (BALF) and apoptosis were observed with in situ DNA tailing technique. Results: The increase in Eos infiltration around the blood vessels occurred within 24 h while that around...

Objective: To investigate the relationship between pulmonary eosinophil (Eos) infiltration and apoptotic dysregulation in asthmatic guinea pigs. Methods: After the model of asthma was established with ovalbumin sensitization in guinea pigs, the dynamic changes of pulmonary Eos infiltration, cytology of braonchoalveolar lavage fluid (BALF) and apoptosis were observed with in situ DNA tailing technique. Results: The increase in Eos infiltration around the blood vessels occurred within 24 h while that around the bronchi and increase of Eos number in BALF persisted for at least 7 d after the sensitization. The rates of Eos apoptosis in asthmatic guinea pigs at the 24th, 48th and 72nd h after the sensitization were respectively (2.1±1.3)%, (3.7±2.3)% and (4.6±2.7)%, which were significantly lower than those in the normal guinea pigs (P<0.05). Conclusion: The Eos infiltration in the lungs is inhibited to result in prolonged increase of Eos, which may play an important role in the pathogenesis of asthma.

目的:探讨支气管哮喘肺内嗜酸性细胞(Eos)增多与Eos凋亡异常的关系。方法:采用卵蛋白致敏的豚鼠哮喘模型和原位细胞凋亡DNA末端标记检测方法,动态观察肺组织Eos浸润、支气管肺泡灌洗液(BALF)细胞学和Eos凋亡的变化。结果:致敏豚鼠抗原激发后,血管周围Eos迁移和浸润主要发生在24h以内,而支气管周围Eos浸润和BALF内Eos增高持续1周以上。哮喘豚鼠第24h,48h,72hEos凋亡率分别为[(2.1±1.3)%,(3.7±2.3)%,(4.6±2.7)%,显著低于正常豚鼠(10.1±3.4)%,P<0.05],至168h才接近于正常水平。结论:哮喘肺内Eos凋亡受到抑制,生存时间延长,导致肺内Eos增多持续存在,在哮喘的发病中可能起着重要作用

Using in situ terminal labeling(ISEL), DNA electrophoresis and immunohistochemical methods, we studied the apoptosis and its mechanism of mouse peripheral lymphocytes induced by 2, 4, 6 and 8 Gy whole body γ irradiation and the expression of bax and bcl 2 gene products related to apoptosis was also studied. It was found that, ① At the early stage after irradiation (4 h ̄7 d) the percentage of lymphocyte apoptosis increased rapidly, for instance, 4 h after 2, 4, 6 and 8 Gy γ irradiation the apoptotic lymphocytes...

Using in situ terminal labeling(ISEL), DNA electrophoresis and immunohistochemical methods, we studied the apoptosis and its mechanism of mouse peripheral lymphocytes induced by 2, 4, 6 and 8 Gy whole body γ irradiation and the expression of bax and bcl 2 gene products related to apoptosis was also studied. It was found that, ① At the early stage after irradiation (4 h ̄7 d) the percentage of lymphocyte apoptosis increased rapidly, for instance, 4 h after 2, 4, 6 and 8 Gy γ irradiation the apoptotic lymphocytes were as 2.6, 3.8, 5.5 and 10.4 times as that of control values, showing a good relationship between apoptosis and radiation doses; ② At the same time, with the increase in radiation doses the absolute counts of peripheral lymphocyte decreased sharply, for instance, 4 h after 2, 4, 6 and 8 Gy γ-irradiation the absolute counts of lymphocytes were 82%, 63%, 47% and 22% when compared with that of control values; ③ Using in situ terminal labeling method the peak value of lymphocyte apoptosis was observed on the 7th day after irradiation, and apoptotic lymphocytes were as 16 times as that of control value, which were in accordance with that obtained by May Grunwald Giemsa staining method. Meanwhile, the absolute counts of peripheral lymphocytes dropped to the lowest value on the 7th day after irradiation, suggesting that lymphocyte apoptosis might be major cause of lymphocyte reduction at the early stage after irradiation. ④ Abnormal expression of bcl 2 and bax gene products in irradiated lymphocytes indicated that they were closely related to the apoptosis in peripheral lymphocytes.

用原位末端标记、DNA电泳和免疫组化技术,观察了经2,4,6和8Gy不同剂量γ-射线照射后,小鼠外周血淋巴细胞的凋亡和与凋亡有关的Bax和Bcl-2蛋白在其中的作用。结果表明:(1)在照射后早期(4h~7d),淋巴细胞凋亡率迅速增加,如2,4,6和8Gy照射后4h,凋亡率分别为对照值的2.6,3.8,5.5和10.4倍,显示出较好的剂量效应关系。(2)随着照射剂量的增加,淋巴细胞绝对数急剧降低,如2,4,6和8Gy照射后4h,淋巴细胞的绝对数分别为对照值的82%,63%,47%和22%,与淋巴细胞凋亡呈相反趋势,提示凋亡可能是急性照射后淋巴细胞减少的主要原因之一。(3)DNA凝胶电泳显示,6Gy照射后3d,淋巴细胞出现特征性的梯形谱;末端标记法显示,6Gy照射后7d,凋亡率达到峰值,为照前值的16倍,这些结果与May-Grunwald-Giemsa染色所得数据基本符合。(4)照射后Bax和Bcl-2蛋白的异常表达证实,二者在淋巴细胞凋亡的调控中起重要作用。上述结果为阐明淋巴细胞辐射损伤与修复的机制,以及急性放射病的防治提供了重要的依据。

 
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