助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   囊泡单胺转运体 的翻译结果: 查询用时:0.523秒
图标索引 在分类学科中查询
所有学科
神经病学
基础医学
更多类别查询

图标索引 历史查询
 

囊泡单胺转运体
相关语句
  vesicular monoamine transporter
     Influence of Paraquat on Vesicular Monoamine Transporter 2 in Nigra-striatum of Mice
     百草枯对小鼠黑质纹状体通路囊泡单胺转运体2的影响
短句来源
     Aim:To study the effect of lactacystin on dopamine ltransporter(DAT)and vesicular monoamine transporter(VMAT2)protein expressions in rats striatum.
     目的:探讨蛋白酶体抑制剂lactacystin对大鼠纹状体多巴胺转运体(DAT)和囊泡单胺转运体2(VMAT2)蛋白表达的影响。
短句来源
     Objective: To investigate the distribution of vesicular monoamine transporter(VMAT2) in PD model of C57BL mice brain and their relationships with Parkinson disease.
     目的:探讨囊泡单胺转运体(VMAT2)在帕金森病(PD)小鼠模型脑组织中的分布特征与帕金森病的关系。
短句来源
     To prepare the RNA probe of Chinese human vesicular monoamine transporter 2(VMAT 2) gene and to detect whether the gene can express in eukaryotic cells, VMAT 2 gene derived from the clone vector pGEM Easy T VMAT 2 was subcloned into vector pBK RSV to construct the expression vector pBK RSV VMAT 2. We used pBK RSV VMAT 2 as a template to synthesize the RNA probe labelled digoxigenin with a transcriptional procedure in vitro, and determined the contents of probes by dot blot.
     本研究目的在于制备人 型囊泡单胺转运体 (VMAT2 )基因的反义和正义 RNA探针 ,以检测 VMAT2 基因能否在真核细胞表达。 从携带 p GEM-Easy-T-VMAT2 克隆载体中通过限制性酶切反应得到 VMAT2 基因 ,与 p BK-RSV载体重组构建真核表达载体 p BK-RSV-VMAT2 ;
短句来源
     Aim:To explore whether the vesicular monoamine transporter 2 (VMAT2) was involved in pathogenic mechanism of parkinsonian mice induced by paraquat. Methods: Apply immuno-histochemistry and in situ hybridization techniques respectively to observe the changes of VMAT2 in striatum and the expression of VMAT2 mRNA in substantia nigra of parkinsonian mice induced by parquat taken orally.
     目的 :探讨囊泡单胺转运体 2 (vesicularmonoaminetransporter 2 ,VMAT2 )是否参与百草枯所致帕金森病 (Parkinson’sdisease ,PD)的发病机制。 方法 :用口服百草枯的途径 ,建立小鼠PD模型 ;
短句来源
更多       
  “囊泡单胺转运体”译为未确定词的双语例句
     AIM:To evaluate the efficiency of vesicular monoamine transporter-2(VMAT2) in promoting the circulating usage of monoamine transmitters by the detection of the contents of monoamine transmitters inside and outside cells.
     目的:获得人Ⅱ型囊泡单胺转运体(vesicularmonoaminetransporter-2,VMAT2)基因,转染至MN9D细胞,通过检测细胞内外单胺类递质含量的变化评价VMAT2基因促进单胺类递质循环利用的效应。
短句来源
     MOLECULAR CLONING OF VESICULAR MONOAMINE TRANSPORTER-2 cDNA FROM HUMAN FETAL BRAIN
     人Ⅱ型囊泡单胺转运体基因的克隆
短句来源
     EFFECT OF OVEREXPRESSING VESICULAR MONOAMINE TRANSPORTER-2 GENE ON THE CONTENTS OF DOPAMINE IN VITRO
     Ⅱ型囊泡单胺转运体基因过表达对多巴胺含量影响的体外研究
短句来源
     Objective: To study the resistance of VMAT2 transgenic CHO cells to the toxicity of dopamine.
     目的:探讨囊泡单胺转运体(vesicular monamine transporter-2,VMAT2)对多巴胺(dopamine,DA)毒性的抵抗作用。
短句来源
  相似匹配句对
     MOLECULAR CLONING OF VESICULAR MONOAMINE TRANSPORTER-2 cDNA FROM HUMAN FETAL BRAIN
     人Ⅱ型单胺转运基因的克隆
短句来源
     The Role of Distribution of Vesicular Monoamine Transporter in Brain Tissue in the Pathogenesis of Parkinson Disease
     单胺转运的分布特征在帕金森病发病机制中的作用
短句来源
     A Study on Antitoxic Role of Vesicular Monoamine Transporter 2 in Transgenic Chinese Hamster Ovary Cells
     转基因中国仓鼠卵细胞中单胺转运抗毒性作用的研究(英)
短句来源
     Influence of Paraquat on Vesicular Monoamine Transporter 2 in Nigra-striatum of Mice
     百草枯对小鼠黑质纹状通路单胺转运2的影响
短句来源
     Distribution of Vesicular Monoamine Transporter in Parkinson Disease Model of C57BL Mice
     单胺转运在帕金森病小鼠脑组织中的分布特征
短句来源
查询“囊泡单胺转运体”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  vesicular monoamine transporter
Localization of vesicular monoamine transporter isoforms (VMAT1 and VMAT2) to endocrine cells and neurons in rat
      
Polyclonal antipeptide antibodies have been raised against each of the two isoforms of the rat vesicular monoamine transporter, VMAT1 and VMAT2.
      
Reserpine- and tetrabenazine-sensitive transport of3H-histamine by the neuronal isoform of the vesicular monoamine transporter
      
The transport of3H-histamine by the endocrine-specific (VMAT1) and neuronal (VMAT2) isoforms of the vesicular monoamine transporter has been evaluated in digitonin-permeabilized fibroblasts transfected with either VMAT1 or VMAT2.
      
Vesicular monoamine transporter type 2 (VMAT2) proteins in nerve terminals take up and partition DA from neuronal cytoplasm into synaptic vesicles.
      
更多          


Objective To obtainthe Chinese human geneof vesicular monoamine transporter 2 (VMAT2) for the treatment of Parkinson's disease Method Total cellular RNA was isolated from human fetal brain and reversely transcribed into cDNA The PCR products were inserted into the pGEM Easy T vector for sequencing after an analysis of the recombinant pGEM Easy T plasmids by restriction enzymes Result The sequence of the target DNA fragment was 1637bp, which matched the size of purpose Conclusion The resultsshow...

Objective To obtainthe Chinese human geneof vesicular monoamine transporter 2 (VMAT2) for the treatment of Parkinson's disease Method Total cellular RNA was isolated from human fetal brain and reversely transcribed into cDNA The PCR products were inserted into the pGEM Easy T vector for sequencing after an analysis of the recombinant pGEM Easy T plasmids by restriction enzymes Result The sequence of the target DNA fragment was 1637bp, which matched the size of purpose Conclusion The resultsshow that the full length cDNA of Chinese VMAT2 with additional Kozak sequence was cloned and could be expressed in eukaryotic cells

目的 为获得人Ⅱ型囊泡单胺转运体 (vesicularmonominetransporter 2VMAT2 )以治疗帕金森病 ,克隆添加Kozak序列的VMAT2cDNA ,用于真核细胞表达。方法 从人胎脑中提取总RNA ,逆转录成cDNA ,设计一对引物用PCR方法扩增目的cDNA片段并将其重组于 pGEM Easy T载体中 ,酶切鉴定插入片段后 ,进行全序列测定。结果 RT PCR扩增到 16 37bp的带有Kozak序列的cDNA片段。结论 克隆人VMAT2cDNA片断 ,可用于真核细胞表达

AIM:To evaluate the efficiency of vesicular monoamine transporter-2(VMAT2) in promoting the circulating usage of monoamine transmitters by the detection of the contents of monoamine transmitters inside and outside cells. METHODS:Total RNA was extracted from human fetal brains.cDNA fragments were a mplified with RT-PCR technique and recombined in PGEM-Easy-T vector for th e complete sequence arrangement.Recombinant eukaryocyte expression vector pBK-RSV-VMAT2 was used to transfect MN9D cells.The expression of...

AIM:To evaluate the efficiency of vesicular monoamine transporter-2(VMAT2) in promoting the circulating usage of monoamine transmitters by the detection of the contents of monoamine transmitters inside and outside cells. METHODS:Total RNA was extracted from human fetal brains.cDNA fragments were a mplified with RT-PCR technique and recombined in PGEM-Easy-T vector for th e complete sequence arrangement.Recombinant eukaryocyte expression vector pBK-RSV-VMAT2 was used to transfect MN9D cells.The expression of VMAT2 was detecte d by immunocytochemistry and the contents of monoamine transmitters were measure d with high pressure liquid chromatog raphy(HPLC). RESULTS:After amplification with RT-PCR,a 1637-bp Kozak sequence was obta ined.In situ hybridization detected that pAAV-VMAT2 expressed in COS7 cells.Im munohistochemistry showed that transgenic MN9D cells in the experimental group w ere stained more obviously than that in the control group.The findings of HPLC p resented that the contents of intracellular and extracellular dopamine were incr eased to(227.2± 20.8)% and(302.6± 15.7)% respectively(P< 0.01),while the c ontents of extracellular homovanillic acid(HVA) and dihydroxyphenyl acetic acid( DOPAC) were decreased to(48.1± 5.3)% and(55.7± 4.9)% (P< 0.05) respectively ,and the contents of intracellular HVA and DOPAC was respectively increased to(3 32.1± 28.2)% and(1 176.8± 82.5)% (P< 0.01). CONCLUSION:VMAT2 can relatively increase the efficiency of recirculation of d opamine and VMAT2 gene may be a promising target gene on gene therapy for Parkin son's disease.

目的:获得人Ⅱ型囊泡单胺转运体(vesicularmonoaminetransporter-2,VMAT2)基因,转染至MN9D细胞,通过检测细胞内外单胺类递质含量的变化评价VMAT2基因促进单胺类递质循环利用的效应。方法:从人胎脑中提取总RNA,RT-PCR方法扩增目的cDNA片段并将其重组于pGEM-Easy-T载体中,进行全序列测定。重组真核表达载体pBK-RSV-VMAT2,转染MN9D细胞,免疫细胞化学检测VMAT2的表达,HPLC检测细胞内外单胺类递质含量的变化。结果:RT-PCR扩增到1637bp的带有Kozak序列的cDNA片段。原位杂交证实pAAV-VMAT2能在COS7细胞表达;免疫组化证实转基因的MN9D细胞(实验组)VMAT2免疫着色明显高于对照组。HPLC结果表明实验组中细胞内外的多巴胺含量分别升高至(227.2±20.8)%和(302.6±15.7)%(P<0.01),而细胞外HVA、DOPAC分别降低至(48.1±5.3)%和(55.7±4.9)%(P<0.05),细胞内HVA,DOPAC分别升高至(332.1±28.2)%和(1176.8±82.5)%(P<0.01...

目的:获得人Ⅱ型囊泡单胺转运体(vesicularmonoaminetransporter-2,VMAT2)基因,转染至MN9D细胞,通过检测细胞内外单胺类递质含量的变化评价VMAT2基因促进单胺类递质循环利用的效应。方法:从人胎脑中提取总RNA,RT-PCR方法扩增目的cDNA片段并将其重组于pGEM-Easy-T载体中,进行全序列测定。重组真核表达载体pBK-RSV-VMAT2,转染MN9D细胞,免疫细胞化学检测VMAT2的表达,HPLC检测细胞内外单胺类递质含量的变化。结果:RT-PCR扩增到1637bp的带有Kozak序列的cDNA片段。原位杂交证实pAAV-VMAT2能在COS7细胞表达;免疫组化证实转基因的MN9D细胞(实验组)VMAT2免疫着色明显高于对照组。HPLC结果表明实验组中细胞内外的多巴胺含量分别升高至(227.2±20.8)%和(302.6±15.7)%(P<0.01),而细胞外HVA、DOPAC分别降低至(48.1±5.3)%和(55.7±4.9)%(P<0.05),细胞内HVA,DOPAC分别升高至(332.1±28.2)%和(1176.8±82.5)%(P<0.01)。结论:克隆了VMAT2cDNA片断,可在真核细胞中表达。该基因的过表达可促进多巴胺的循环利用,有望用于帕金森病的基因治疗。

To prepare the RNA probe of Chinese human vesicular monoamine transporter 2(VMAT 2) gene and to detect whether the gene can express in eukaryotic cells, VMAT 2 gene derived from the clone vector pGEM Easy T VMAT 2 was subcloned into vector pBK RSV to construct the expression vector pBK RSV VMAT 2. We used pBK RSV VMAT 2 as a template to synthesize the RNA probe labelled digoxigenin with a transcriptional procedure in vitro, and determined the contents of probes by dot blot. After the recombinant...

To prepare the RNA probe of Chinese human vesicular monoamine transporter 2(VMAT 2) gene and to detect whether the gene can express in eukaryotic cells, VMAT 2 gene derived from the clone vector pGEM Easy T VMAT 2 was subcloned into vector pBK RSV to construct the expression vector pBK RSV VMAT 2. We used pBK RSV VMAT 2 as a template to synthesize the RNA probe labelled digoxigenin with a transcriptional procedure in vitro, and determined the contents of probes by dot blot. After the recombinant vector was transiently transfected to COS 7 cells, the expression of VMAT 2 gene was detected by in situ hybridization and immunocytochemistry. The contents of antisense and sense probe were 80 ng/μl and 120 ng/μl respectively, and the ratio of positive cells of the gene in transfected COS 7 cells was (10.6±1.2)%. The results showed that the sensitive VMAT 2 RNA probes were obtained, and the Chinese VMAT 2 gene can be expressed in eukaryotic cells.

本研究目的在于制备人 型囊泡单胺转运体 (VMAT2 )基因的反义和正义 RNA探针 ,以检测 VMAT2 基因能否在真核细胞表达。从携带 p GEM-Easy-T-VMAT2 克隆载体中通过限制性酶切反应得到 VMAT2 基因 ,与 p BK-RSV载体重组构建真核表达载体 p BK-RSV-VMAT2 ;分别以 T7和 T3聚合酶制备反义和正义探针 ,通过斑点杂交检测探针浓度。转染猴肾成纤维细胞COS-7,原位杂交及免疫荧光细胞化学检测 VMAT2 的表达。结果证明 ,反义和正义探针浓度分别为 80 ng/μl及 12 0 ng/μl;原位杂交及免疫组织化学证实重组的真核表达载体 p BK-RSV-VMAT2 在 COS-7的表达阳性率为 (10 .6± 1.2 ) %。本研究结果表明获得 VMAT2 基因反义链及正义链 RNA探针 ,并检测到 VMAT2 基因在真核细胞中表达

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关囊泡单胺转运体的内容
在知识搜索中查有关囊泡单胺转运体的内容
在数字搜索中查有关囊泡单胺转运体的内容
在概念知识元中查有关囊泡单胺转运体的内容
在学术趋势中查有关囊泡单胺转运体的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社