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猫肾细胞
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  feline kidney cells
     Kinetics of canine Parvovirus Ams 20 Multiplication in Primary Feline Kidney Cells
     犬细小病毒在乳猫肾细胞中的增殖动态
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  “猫肾细胞”译为未确定词的双语例句
     In this study, canine parvovirus strain HL-1 was propagated on F81 cell.
     本研究利用猫肾细胞(F81)繁殖了并扩增了分离得到的HL-1 株VP2 基因。
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     F_(65) of cat kidney cell lineage inoculates with 2×10~(-2) of RPV—H synchronical y, and cultivation at 37℃. when the cytopathic effect is more than 75%, to collect all of them.
     以RPV-H为种毒,按2×10~(-2)接毒量,采用同步接毒的方法,接于猫肾细胞系F_(65)株,于37℃静置培养,待细胞病变(CPE)达到75%以上时,开始收获。
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     RESULTS Of the five strains of Vero XAYU, Vero XAYE, Vero XAH, Vero XAJN and Vero XAM in our laboratory, 3 strains of F 81 XAJU, F 81 XAM,and F 81 XAK grew anchor free without carcinogenesis, but 5 strains of MDCK XAYE,MDCK XAYU,MDCK XAJN,MDCK XAJA,and MDCK XAM grew anchor free with lower carcinogenesis;
     结果 本实验室的五株非洲绿猴肾细胞 Vero- XAYU ,Vero- XAYE,Vero- XAH,Vero- XAJN和 Vero- XAM,三株猫肾细胞 F81 -XAJU,F81 - XAM和 F81 - XAK不呈现致瘤性独立抛锚生长 ; 犬肾细胞 MDCK- XAYE,MDCK- XAYU,MDCK- XAJN,MD-CK- XAJA和 MDCK- XAM五株则呈现较低的致瘤性独立抛锚生长 ;
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     The expressed products were extracted from the gel and added as immunogen to immunize mice intraperitoneally, 5 doses with one-week intervals. The specificity of antibody was detected positively against CPV-infected FK81 cell in the indirect immunofluorescent assay (IFA).
     将表达产物从SDS-PAGE凝胶中切出回收,并用其免疫6-8周龄ICR小鼠,每周免疫一次,5次免疫后,采血分离血清,所得抗血清与犬细小病毒扬州分离株感染的胎猫肾细胞(FK81)进行间接免疫荧光试验(IFA),呈现特异性荧光,表明大肠杆菌表达的VP2基因产物保留了天然VP2蛋白的抗原性。
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     Using the high speed centrifugation, PEG precipitation, sucrose and CsCl density gradient centrifugation, and CsCl equilibrium density centrifugation, the virus particles were purified from the feline kidney cell culture infected mink enteritis virus.
     应用高速离心—PEG沉淀—蔗糖和氯化铯密度离心—氯化铯平衡密度梯度离心等方法,从水貂肠炎病毒猫肾细胞培养物中提纯病毒粒子。 提纯的病毒粒子分为氯化铯浮密度为1.32~1.36g/ml的空壳病毒粒子和氯化铯浮密度为1.40~1.42g/ml的实心病毒粒子。
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  相似匹配句对
     The Subtypes of Renal Cell Carcinoma
     细胞癌亚型
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     The Cat
    
短句来源
     Kinetics of canine Parvovirus Ams 20 Multiplication in Primary Feline Kidney Cells
     犬细小病毒在乳细胞中的增殖动态
短句来源
     Bilateral renal cell carcinoma: report of 6 cases
     双侧细胞
短句来源
     Study of in situ Polymerase Chain Reaction Assay for Detection of Canine Parvovirus(CPV) in Feline Kidney F_(81) Cells
     F_(81)传代细胞中犬细小病毒原位PCR检测方法
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  feline kidney cells
A promoter conversion retroviral vector carrying the WAF-1/CIP-1/SDI-1 gene under the transcriptional control of the glucocorticoid inducible promoter of mouse mammary tumor virus was used to infect human bladder carcinoma or feline kidney cells.
      
Low temperature compartment formation in feline immunodeficiency virus-infected and uninfected feline kidney cells
      
Adherent Crandall-Reese feline kidney cells enclosed in conjugate capsules of 141?±?23?μm diam.
      
Electron microscopic observation of feline kidney cells infected with a feline calicivirus
      
Properties of Aleutian disease virus assayed with feline kidney cells
      
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A serious infectious disease of cat characterized by depression, anorexia, vomiting, diarrhea,bloody stool, fever and leucopenia was investigated.And a strain of feline panleukopenia virus--FNF8 from a field case wasisolated in feline kidney cell culture.The disease might occur at any time of the year, but most outbreaks occurred in the winter and spring ( 258/371 ) . Male or female cats at any age might be infected, but the disease was encounted more often in younger cats ( 295/371 ) .Mortality rate of the...

A serious infectious disease of cat characterized by depression, anorexia, vomiting, diarrhea,bloody stool, fever and leucopenia was investigated.And a strain of feline panleukopenia virus--FNF8 from a field case wasisolated in feline kidney cell culture.The disease might occur at any time of the year, but most outbreaks occurred in the winter and spring ( 258/371 ) . Male or female cats at any age might be infected, but the disease was encounted more often in younger cats ( 295/371 ) .Mortality rate of the disease was high.The virus resisted acid(pH3.0), heat ( 50℃, 60min ) and ether. Viral replication was inhibited by 5-bromo-2'-deoxyuridine ( BUDR). By electron microscopy, the virions were spherical or icosahedral in shape ,24-28nm in diameter, with no envelope. Its density was between 1.29-1,34g/cm3. With hematoxylin eosin stain, dark-red, cowdry type A intranuclear inclusion bodies were detected in the infected cells. The virus agglutinated swine erythrocytes at 4℃, and was agglutinated by standard antiserum of feline panleukopenia virus by immune electromicroscopy.

本文调查了猫科动物一种以倦怠、厌食、呕吐、下痢、血便、体温升高和白细胞数减少为特征的烈性传染病的发病规律和流行情况。利用猫肾细胞培养,从自然病例中分离出我国第一株猫泛白细胞减少症病毒——FNF8毒株。从而证实了我国有猫泛白细胞减少症的存在。

Thirteen dogs 2--3 months of age were used in the study andexpulsion of parasites in the alimentary canal was done for 10 of these dogs.Food was withheld for 24 and 48 hours before and after virus inoculationrespectively. CPV at the level of 4th passage in filine kidney cell cultureand with 4×10~(5·75) TCID_(50)/ml was inoculated by intraoronasal, intravenousor intramuscular route separately, the dose is 11. 5 or 25.0 ml of culturefluid per kg of boody weight. From 4th day onwards affer exposure all thesedogs...

Thirteen dogs 2--3 months of age were used in the study andexpulsion of parasites in the alimentary canal was done for 10 of these dogs.Food was withheld for 24 and 48 hours before and after virus inoculationrespectively. CPV at the level of 4th passage in filine kidney cell cultureand with 4×10~(5·75) TCID_(50)/ml was inoculated by intraoronasal, intravenousor intramuscular route separately, the dose is 11. 5 or 25.0 ml of culturefluid per kg of boody weight. From 4th day onwards affer exposure all thesedogs showed typica1 signs one by one, and revealed coproconversion of hemag-glutination. CPV particles were seen in their feces by EM negative staining.There were hemorrhagic inflammation of small intestines, myocardial localhemorrhage and granular degeneration. Eoth thymocytes in cortex of thymusand lymhopcytes in cortex of lymph nodes were dead and decreased in number.Eosinophilic, basophilic or amphichromatic intranuclear inclusion bodies wereseen in some crypt epithelia of small intestines and in lymphocytes and reticu-lar cells of lymph nodes and thymocytes and epithelialreticular cells of thymus.Diaphragms of 3 puppies at the age of 3 months hemorrbaged locally. Underscanning EM, villi of small intestines were swelled and the transverse groovesaround villi were shallow and wide or completely lost, globlet cell mouthsdilated and increased in number. CPV of 27 nm in diameter were found innuclei of the nondifferentiated crypt epithelial cells of small intestines andits morphogenesis was described.

取2~3月龄大13只(10只肠道驱虫),禁食24h后,分组分别经口鼻、静脉、肌肉接种TCID_(50)/ml为4×10~(5·75)的犬细小病毒(CPV)第四代猫肾细胞培养毒11.5或25.0m1/kg,再禁食48h。结果,各犬均于接种后第4d起陆续典型发病,粪便HA试验阳性,负染见CPV粒子。主要病理学变化是,小肠出血性坏死性炎,心肌灶状出血和颗粒变性;胸腺皮质的胸腺细胞及淋巴结皮质的淋巴细胞死亡、减少;在一些小肠隐窝上皮细胞、淋巴结的淋巴细胞和网状细胞以及胸腺的胸腺细胞和上皮性网状细胞核内见嗜酸性、嗜硷性或两染性包涵体;3只3月龄犬膈肌灶状出血。小肠粘膜扫描电镜观察,粘液增多,绒毛肿胀。其上的横沟变钱或消失。杯状细胞开口扩张,数目增加。电镜观察,在小肠隐窝上皮未分化细胞的核内见直径为27nm的CPV粒子,并对其形态发生作了描述。

Canine parvovirus particles were observed in negatively stained preparations of faecal samples from an experimentally infected dog with acute diarrhea. Virus isolated from this dog was inoculated into primary feline kidney cells, and wide spread intranuclear inclusion bodies were detected at 6~12 days postinfection. The culture fluids agglutinated porcine erythrocytes at 4℃ and this reation was inhibited by c. p. v. antiserum. The agglutination titer increased gradually during the days after infection. Further...

Canine parvovirus particles were observed in negatively stained preparations of faecal samples from an experimentally infected dog with acute diarrhea. Virus isolated from this dog was inoculated into primary feline kidney cells, and wide spread intranuclear inclusion bodies were detected at 6~12 days postinfection. The culture fluids agglutinated porcine erythrocytes at 4℃ and this reation was inhibited by c. p. v. antiserum. The agglutination titer increased gradually during the days after infection. Further studies showed that the infected culture fluids could also agglutinate monkey and cat erythrocytes. In the immunoperoxidase studies, specifically stained substance was detected first in the nuclei 14 h postinfectiono and its intensity reached the maximum 72 h postinfection, since then viral antigen was also observed in the cytoplasm. Electron microscopic examination of the infected cells showed parvovirus particles and intranuclear inclusions. Im mune electron microscopic examination of the infected culture fluids revealed aggregates of virus particles(20 nm ± in diameter).

本文记述了犬细小病毒在乳猫肾细胞内生长情况。试验中观察了包涵体形成的过程与细胞培养液内病毒血凝滴度的联系;用亲和素-生物素酶间接染色法证明细胞内的变化是特异的;在4℃pH7.2条件下病毒液对猪血球、猴血球、猫血球均可产生血凝现象,且对猪血球凝集效价最高,而用犬细小病毒阳性血清与病毒液作用,可抑制此血凝现象;电镜检查感染细胞,发现胞核变化较明显,将感染细胞培养液作免疫电镜检查,可见聚集的细小病毒颗粒。

 
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