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rna分子     
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  rna molecules
     microRNAs(miRNAs) are about 20~24-nucleotide,small single-strand non-coding RNA molecules which derived from pri-miRNAs containing hairpin structure through RNaseⅢ-like enzyme Dicer.
     microRNAs(miRNAs)是一类长约20~24个核苷酸的单链非编码小RNA分子,由一段具有发夹结构的70~80个核苷酸长度的单链RNA前体(pri-mRNAs)在类RNaseⅢ酶Dicer的剪切下而生成.
短句来源
     The present study introduces circulating viral transcripts as a potential parameter. The four overlapping ORFs on viral DNA, i.e., PreC/C, polymerase, PreS1/PreS2/S, and X, are transcribed into genomic and subgenomic RNA molecules.
     HBV DNA有4个重叠的开放式阅读框架(Open Reading Frame,ORFs),即:PreC/C、多聚酶、PreS 1/PreS2/S和X基因,其可被转录到基因组和前基因组RNA分子中。
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     In proper conditions of reaction, NS5B can initate many RNA molecules to promote RNA reproduction. Particularly it can effectively reproduce HCV (+) RNA.
     在合适的反应条件下,NS5B可以以多种RNA分子为模板催化RNA复制,特别是能有效复制HCV全长(+)RNA。
短句来源
     In vivo expression of engineered functional RNA molecules,such as short interfering (si) RNAs,aptazymes,maxizymes and intramers,can regulate gene function at the mRNA or protein level.
     人工构建的siRNAs、aptazymes、maxizymes以及intramers等功能RNA分子,可以在mRNA或蛋白质水平上调控基因的功能.
短句来源
     RNA silencing is a conserved eukaryotic pathway involved in suppression of gene expression via sequence-specific interactions that are mediated by 21-26nt RNA molecules.
     RNA沉默是一种由21-26nt RNA分子介导的真核生物体内普遍存在的以序列特定性行为 抑制基因表达的保守途径。
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  rna molecular
     Progressing of RNA molecular expression technology
     RNA分子表达技术研究进展
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     With the development of biotechnology, more and more RNA molecular expression technologies have been established.
     随着生物技术的发展,越来越多的RNA分子技术被建立起来,为基因克隆和基因表达研究提供了有利的工具。
短句来源
     RNA molecular is composed of the four nucleotids A, C, G, U. RNA secondary structure is a single-stranded chain of the nucleotide in which part of the molecule can base-pair with a complementary nucleotide in another part by folding oneself. Based on the relationship between the structure and function, a lot of research have been performed on predicting base-pair which is indispensable to the RNA three-dimensional strcutrue.
     RNA是由四种普通的核苷酸(腺嘌呤A、鸟嘌呤G、胞嘧啶c和尿嘧啶U)组成,单链的RNA分子通过自身回折,使链中分子中的一部分核苷酸可与其它部分核苷酸互补配对,即RNA的二级结构。
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     The result of polyacrylamid denatured gel electrophoresis showed: the multimeric ribozymes caused self cleaving during the transcription reaction in vitro and formed RNA step ladders from 70 nt to 706 nt, which indicates that the self cleavage ribozyme transcripts can be used as RNA molecular mass markers.
     5%聚丙烯酰胺变性凝胶电泳结果显示 :体外转录过程中多体酶发生了自切割 ,核酶转录物切割成从 70nt到 70 6nt的RNA等梯度带 ,因此 ,可以作为RNA分子质量标记之用
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     RESULTS Ribozyme is a kind of RNA molecular which possess site specific clevavage and catalytic potential.
     结果 核酶是一种具有位点剪切和催化潜能的RNA分子
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  rna molecule
     Adopting this software for the microcomputer of IBM PC-XT type the second order structure of RNA molecule with 1000 alkaline bases is drawn in about 3 minutes while that with 1500 alkaline bases is drawn in about 5 minutes.
     使用此软件在速度较慢的IBM PC-XT型微机上,绘制1000个碱基的RNA分子二级结构图约用3分钟,绘制1500个碱基的RNA分子二级结构图约用3分钟。
短句来源
     The yeast three-hybrid have three components: a protein fused with DNA binding domain、 a hybrid RNA molecule、 a protein fused with DNA activation domain.
     酵母三杂交系统由三部分组成:DNA结合结构域融合蛋白、杂交RNA分子、DNA激活结构域融合蛋白。
短句来源
     The up-to-date implication of gene is the integrated DNA section of coding a RNA molecule or protein molecule.
     基因的现代含义为:编码一个RNA分子或蛋白质分子的完整的DNA片段。
短句来源
     Drawing of Intellectual Faculties for Second Order Structure of RNA Molecule
     RNA分子二级结构智能绘图
短句来源
     Realizing this approach by means of software the second order structure of RNA molecule represented by matched pair of alkaline base can become automatically picture play and give the picture output directly by printer.
     用软件实现此方法,可以把以碱基匹配对表示的RNA分子二级结构,自动转化为图形显示,并直接由打印机输出图形。
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  rna dot
     The expression of three heat shock proteins (HSPs)-HSP 90a, HSP 70 and HSP 27 in celis obtained from 22 patients with leukemia, erythroleukemia cell line K562, and normai blood celis was observed by means of RNA dot blot analysis.
     运用RNA分子杂交的方法,观察了热休克蛋白(heat shock protein,HSP)90α、70、27在22例白血病病人细胞,正常血细胞及K562红白血病细胞系中的表达。
短句来源
     The expression of three heat shock proteins(HSPs)HSP90α, HSP70, HSP27 in cells obtained from 22 patients with leukemia, K562 erythroleukemia cell line, and normal blood cells were observed by means of RNA Dot Blot analysis.
     运用RNA分子杂交的方法,观察了热休克蛋白(heatshockprotein,HSP)90α、70、27在22例白血病病人细胞、正常血细胞及K562红白血病细胞系中的表达。
短句来源
     Methods Immunohistochemical technique was used to identify the expression of bcl 2 and bax proteins in HL 60 cells,as well as RNA dot blot hybridization to show the expression of bcl 2 mRNA in HL 60 cells.
     方法运用免疫组织化学技术探讨14 7Pm对HL 60细胞bcl 2 /bax的蛋白表达 ,以及RNA分子杂交技术探讨14 7Pm对HL 60细胞bcl 2mRNA的转录水平表达。
短句来源
     Immunohistochemical technique was used to identity the expression of bcl\|2 and bax proteins in HL\|60 cells,meanwhile RNA dot blot hybridization was used to show the expression of bcl\|2 mRNA in HL\|60 cells.
     运用免疫组织化学技术探讨浓缩铀对HL 6 0细胞bcl 2 bax的蛋白表达 ,以及RNA分子杂交技术探讨浓缩铀对HL 6 0细胞bcl 2mRNA的转录水平表达。
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      rna molecules
    RNA interference is a phenomenon associated with gene suppression via regulatory RNA molecules, which are common in plants, animals, and fungi.
          
    Transcription of RNA molecules from synthetic DNA templates with T7 RNA polymerase is a common procedure for the preparation of long RNA molecules.
          
    We aimed at combining both chemical and enzymatic procedures to synthesise RNA molecules by RNA primed transcription with T7 RNA polymerase.
          
    Interaction between RNA molecules of a two-component trans analog of antigenomic HDV ribozyme
          
    A set of imported RNA molecules varies in different organisms.
          
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      rna molecular
    Finger print analysis of bacterial 16S RNA (molecular phylogeny) has not only changed the classification of bacteria but also the approach to solving environmental problems.
          
    Differences are explained in terms of specific features of RNA molecular structures.
          
    The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization.
          
    For calibration, digoxigenin-labeled RNA molecular weight marker I from Roche Diagnostics was used.
          
    Molecular weights of transcripts were estimated by comparison with RNA molecular weight standards.
          
    更多          
      rna molecule
    It was believed until recently that the only possible mechanism of RNA recombination is replicative template switching, with synthesis of a complementary strand starting on one viral RNA molecule and being completed on another.
          
    Alphaviruses are enveloped viruses containing a single positive strand RNA molecule as genome.
          
    Our results suggest that in pulling experiments on RNA molecule containing tertiary structures, the details of the single kinetic barriers can only be obtained using a low pulling rate value, or in the high force regime.
          
    This review discusses the effects the secondary structure of an RNA molecule has on the inherent reactivity of its phosphodiester bonds, and on the catalytic activity of metal ion-based cleaving agents.
          
    The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity.
          
    更多          
      rna dot
    The expression of three heat shock proteins (HSPs)-HSP90α, HSP70, HSP27 in cells obtained from 22 patients with leukemia, K562 erythroleukemia cell line, and normal blood cells was observed by means of RNA dot blot analysis.
          
    The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method.
          
    We analyzed, by RFLP and RNA dot blots, cosegregation between clones for the env gene of endogenous viruses as the most promising candidate for involvement in driving SAT; we did not find differences at the DNA and RNA levels.
          
    The DNA microarray results were corroborated with RNA dot blotting.
          
    After inoculation of SbDV by the aphids, most T2 plants of this transgenic line remained symptomless, contained little SbDV-specific RNA by RNA dot-blot hybridization analysis and exhibited SbDV-CP-specific siRNA.
          
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    The conditions for the reaction of ENA with FDNB was reported, and the relation between the degree of degradation of the RNA and the amount of DNP group combined was studied.The combining site of DNP-in S-RNA was studied by means of ultraviolet absorption spectra, and degradation by RNase and snake venom phosphodiesterase. The results suggested that DNP group combined only with the phosphomonoester of the terminal 5'-phospho guanosine of S-RNA.The possibilities of using the amount of DNP-combined for the calculation...

    The conditions for the reaction of ENA with FDNB was reported, and the relation between the degree of degradation of the RNA and the amount of DNP group combined was studied.The combining site of DNP-in S-RNA was studied by means of ultraviolet absorption spectra, and degradation by RNase and snake venom phosphodiesterase. The results suggested that DNP group combined only with the phosphomonoester of the terminal 5'-phospho guanosine of S-RNA.The possibilities of using the amount of DNP-combined for the calculation of chain length and molecular weight of S-RNA and using the DNP-as a label for the pG……terminal of S-RNA to facilitate the nucleotide sequence analysis from that end are suggested.

    (一)本工作报告了FDNB与RNA的作用条件,并研究了不同断裂程度的RNA和FDNB的反应。(二)对DNP-在大肠杆菌S-RNA分子中的结合位置通过紫外线吸收光谱和牛胰RNase以及蛇毒磷酸双酯酶的降解作用做了探讨,由实验结果推断:DNP-似结合在S-RNA末端5′-G核苷酸的磷酸单酯上。(三)本文说明DNP-的结合量可以用来计算S-RNA的链长及分子量;也可以利用DNP-标记pG末端,以便利于该末端核苷酸排列顺序的研究。

    For the fractionation of ribonucleic acid(ENA) various chromatographic techniques have been reported. In our laboratory the calcium phosphate column chromatography was used for such purpose. It was found that the chromatograms repeatedly obtained from the aqueous phase of the cold phenol extraction of rat liver showed four dominate peaks, designated as A, B, C and D. The properties of these fractions were studied, that A was identified to consist chiefly of the free nucleotides and possibly some oligonucleotides;...

    For the fractionation of ribonucleic acid(ENA) various chromatographic techniques have been reported. In our laboratory the calcium phosphate column chromatography was used for such purpose. It was found that the chromatograms repeatedly obtained from the aqueous phase of the cold phenol extraction of rat liver showed four dominate peaks, designated as A, B, C and D. The properties of these fractions were studied, that A was identified to consist chiefly of the free nucleotides and possibly some oligonucleotides; C, the soluble ENA and D, the ribosomal RNA. The characteristic of B is not clear, and it gives the value of E_(258mμ):E_(280mμ) around 4 and seems to be unstable during dialysis. The stability of the RNA extract by different treatments was examined in the calcium phosphate column and it was found that no significant change of the size of ribosomal RNA(either dissociation or degradation) could be counted to the column itself. So it is suggested that this method of fractionation of RNA's can be used to reflect the state of RNA molecules originally present in solution.On this basis, the RNA extracts from various rat and mouse tissues, normal or abnormal, were analyzed and the different chromatograms were obtained. In the case of encephalomyocarditis virus RNA is isolated from infected brain of mice, the infectivity, although less active than the original material, was still retained in one of the fractions of ENA eluted.

    (1)本实验探索了磷酸钙柱层析在分离动物组织RNA提取液的洗脱条件。(2)磷酸钙柱层析应用于大鼠肝RNA总溶液的分离获得重复性较好的柱层析图谱。(3)对大鼠肝磷酸钙柱层析各峰的RNA性质进行初步分析,但对B峰的性质还不了解。(4)磷酸钙柱层析本身似对RNA分子的降解无甚影响。(5)比较了正常肝、再生肝及3′-甲基奶油黄诱发肝癌RNA的磷酸钙柱层析图谱。(6)观察了大鼠腎、胰、脑等组织RNA的磷酸钙柱层析图谱,与肝脏者有明显的差别。(7)观察了感染脑心肌炎病毒小鼠脑RNA的柱层析图谱,并试验了所洗脱的RNA的部分的致病活性。

    Based upon the characteristic reaction of S-RNA with FDNB which resulted in the attachment of the DNP-group only on the 5'-phosphomonoester end group of S-RNA, this report described experiments which utilized the DNP-group as label for the identification of the terminal nucleotides and their adjacent nucleotide sequences.Both the labeled (DNP-S-RNA) and the ordinary S-RNA were digested with pancreatic ribonuclease, and the resulting products were separated by Rushizky and Knight's paper electrophoresis-chromatography...

    Based upon the characteristic reaction of S-RNA with FDNB which resulted in the attachment of the DNP-group only on the 5'-phosphomonoester end group of S-RNA, this report described experiments which utilized the DNP-group as label for the identification of the terminal nucleotides and their adjacent nucleotide sequences.Both the labeled (DNP-S-RNA) and the ordinary S-RNA were digested with pancreatic ribonuclease, and the resulting products were separated by Rushizky and Knight's paper electrophoresis-chromatography mapping procedure. The substances in the major spots of the map so obtained were identified and estimated by spectrophotometry after elution. Results showed no significant differences in the electrophoretic and chromatographic behaviours between DNP-S-RNA and S-RNA. The terminal oligonucleotides labeled with DNP-group (DNP-oligonucleotides) overlapped with the correspondent oligonucleotides on the map. Identification of the DNP of ultraviolet absorption spots were made by spectrophotometry at 350-370mμ after degradation of the eluate with alkali. The results showed that the terminal nucleotides of 5'-phosphomonoester of E. coli S-RNA were pGp···, pAp···pCp···and trace amounts of pUp···.The sequence adjacent to the pXp seemed to differ for different specific S-RNA. Some of them were found to be pGpUp···, pGpCp···, (pGpAp) Op···, (pGpGpAp) Up···,pApCp···, pApUp··· and pGpGpUp··· and/or(pGpAp) Up···.The oligonucleotides consisting of 4 or more nucleotides were not determined.DNP was left in the original spot of the map, indicating the presence of terminal fragments probably made up of more than four purine nucleotides, and analyses of the spot showed that guanylic acid dominated.

    (1)比较了E.coli DNP-S-RNA和S-RNA的紫外光谱,并研究了DNP-S-RNA的RNase I的降解条件,结果表明两者无显著差别。(2)对DNP-S-RNA的RNase I降解产物的双向电泳层析图谱进行了分析,并与S-RNA图谱进行了比较,两者图谱中光吸收点的分布一致,相应点经洗脱后,由光谱分析与核碱组成测定证明均含有相同的核苷酸组成。(3)在DNP-S-RNA和S-RNA于同一条件下得到的图谱中,前者的某些光吸收点中含有不同量的DNP-。根据DNP-可以标记S-RNA末端5′-磷酸单酯,分析了DNP-结合物,说明在E.coli S-RNA分子中,B末端核苷酸除pGp…以外,尚有pAp…,pCp…以及痕量的pUp…。和末端相邻的核苷酸排列形式,随特异S-RNA而有不同,有pGpUp…,pGpCp…,p(GpGpAp)Up…,p(GpAp)Up…+pGpGpUp…,p(GpAp)Cp…,pApUp…和pApCp…。其他四核苷酸以上多核苷酸未分析。由于电泳和层析后原点部分标记了DNP-,估计末端核苷酸排列有五、六核苷酸以上的多嘌呤核苷酸的排列形式,其中以G为主。

     
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