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   转基因植株再生 的翻译结果: 查询用时:0.133秒
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转基因植株再生
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  regeneration of transgenic plants
     TRANSFORMATION OF WHEAT PROTOPLASTS BY BYDV CP GENE AND THE REGENERATION OF TRANSGENIC PLANTS
     BYDV CP基因转化小麦原生质体及转基因植株再生
短句来源
     Genetic Transformation of Phytase Gene and Regeneration of Transgenic Plants in Cotton
     植酸酶基因转化棉花及转基因植株再生
短句来源
     Genetic Transformation of Isopentenyl Transferase Gene to Tomato and Regeneration of Transgenic Plants
     异戊烯基转移酶基因导入番茄及转基因植株再生
短句来源
     Genetic Transformation of Cotton with Isopentenyl Transferase Gene and Regeneration of Transgenic Plants
     异戊烯基转移酶基因转化棉花及转基因植株再生
短句来源
     Regeneration of transgenic plants of Poncirus trifoliata Raf containing CTV-cp .
     柑桔衰退病病毒外壳蛋白基因转入枳壳及转基因植株再生研究
  transgenic plant regeneration
     Genetic Transformation of Antisense cDNA of Polygalacturonase in Tomato and Transgenic Plant Regeneration
     番茄多聚半乳糖醛酸酶反义cDNA克隆的遗传转化与转基因植株再生
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     Transgenic Plant Regeneration from Wheat (Triticum aestivumL.) Mediated by Agrobacterium tumefaciens
     Transgeni根癌农杆菌介导的小麦转基因植株再生(英文)
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     Genetic transformation of Trifolium repens using Agrobacterium tumefaciens and transgenic plant regeneration with high efficiency
     农杆菌介导白三叶草高效遗传转化和转基因植株再生
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  “转基因植株再生”译为未确定词的双语例句
     Transformation with Genes gag and gp120 from HIV-1 into Tomato and Regeneration of Transgenic Plant
     HIV-1 gag基因和gp120基因转化番茄及转基因植株再生
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     Tomato Transformation and Plant Regeneration by Agrobacterium Harboring Binary Vector Carrying IPT and etr1-1 Genes
     农杆菌介导IPT和etr1-1双基因转化番茄及转基因植株再生
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     PCR tests indicated the recombinant segments of pC54P-GUS, pC77P-GUS, pC309P-GUS, pCU54S and pCU54A have been integrated into rice genome. Detection of
     转pC309P-GUS植株尚未抽穗,但在分蘖期的根、茎、叶中尚未检测到GUS活性。 转基因阳性植株太少,对pCU54S、pCU54A转基因TO植株的田间表型调查尚待这两个载体新的转基因植株再生
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     Genetic Transformation of the Commercial Apple Cultivar New Jonagold and Regeneration of Its Transgenic Plants
     新乔纳金苹果遗传转化及转基因植株再生
短句来源
     1.Establishing the receptor system for ramie genetic transformation.
     1.建立了高效的苎麻转基因植株再生体系
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  regeneration of transgenic plants
Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa.
      
Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the
      
Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S.)
      
Regeneration of transgenic plants is not required as transgenic nodules are formed onAgrobacterium rhizogenes incited roots inoculated withRhizobium.
      
A two step selection procedure is described for high frequency transformation and regeneration of transgenic plants by coculture of leaf discs of Datura innoxia with Agrobacterium tumefaciens carrying binary vectors.
      
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  transgenic plant regeneration
Efficient transgenic plant regeneration throughAgrobacterium-mediated transformation of Chickpea (Cicer arietinum L.)
      
In contrast to previous reports, embryogenic calluses were used as the explant for Agrobacterium infection and transgenic plant regeneration.
      
High-efficiency transformation of Lycium barbarum mediated by Agrobacterium tumefaciens and transgenic plant regeneration via so
      
Agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in Chamaecyparis obtus
      
High efficient transgenic plant regeneration from embryogenic calluses of Citrus sinensis
      
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Transgenic plants were regenerated from soybean ( Glycine max (L.) Merr.) immature cotyledons bombarded by gene_gun with plasmid pGB14AB which contained Bacillus thuringiensis Berliner insecticidal crystal protein gene ( Bt gene )and GUS gene, and from cotyledon nodes infected with LBA4404 which contained pGB14AB. Immature cotyledons of “Zhonghuang No. 4” (variety) and 8502 (line) differentiated many more somatic embryos than others. Desiccation treatment of somatic embryos from immature cotyledons...

Transgenic plants were regenerated from soybean ( Glycine max (L.) Merr.) immature cotyledons bombarded by gene_gun with plasmid pGB14AB which contained Bacillus thuringiensis Berliner insecticidal crystal protein gene ( Bt gene )and GUS gene, and from cotyledon nodes infected with LBA4404 which contained pGB14AB. Immature cotyledons of “Zhonghuang No. 4” (variety) and 8502 (line) differentiated many more somatic embryos than others. Desiccation treatment of somatic embryos from immature cotyledons of “Zhonghuang No. 4” improved significantly their germination frequency. Preculturing of immature cotyledon was beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn. High efficiency of transformation is related to selection of genotype and recipient, desiccation treatment of somatic embryos and improvement of transformed system in soybean.

用苏云金芽孢杆菌(BacillusthuringiensisBerliner)杀虫晶体蛋白(Bt)基因和葡糖苷酸酶(GUS)基因通过基因枪轰击和根癌土壤杆菌(Agrobacteriumtumefaciens(SmithetTownsend)Conn)介导转入大豆(Glycinemax(L.)Merr.),诱导大豆转基因植株再生。大豆主栽品种“中黄4号”和品系8502未成熟子叶有较强体细胞胚分化能力。体细胞胚的脱水处理显著促进“中黄4号”体细胞胚的萌发。未成熟子叶的预培养有利于根癌土壤杆菌感染子叶外植体体细胞胚的分化。基因型和受体的选择,转基因体系的改进,体细胞胚的脱水处理等是提高大豆转基因效率的重要因素。

Transient and stable β-glucuronidase (GUS) expressions and transgenic plant regeneration from the etiolated internode explant of' Royal Cala' apple (Malus domestica Borkh. ) were examined using Agrobacterium tumefaciens supervirulent strains EHA105(p35sGUS intron) during transformation. Explants with auxins (IBA, NAA) yeilded more than twice as many GUS-expressing zones and calli compared with ones on the medium without auxin. All etiolated internodes exhibited GUS expressing zones and yielded 4-fold as many...

Transient and stable β-glucuronidase (GUS) expressions and transgenic plant regeneration from the etiolated internode explant of' Royal Cala' apple (Malus domestica Borkh. ) were examined using Agrobacterium tumefaciens supervirulent strains EHA105(p35sGUS intron) during transformation. Explants with auxins (IBA, NAA) yeilded more than twice as many GUS-expressing zones and calli compared with ones on the medium without auxin. All etiolated internodes exhibited GUS expressing zones and yielded 4-fold as many GUS expressing zones compared to commonly used leaf explants from nonetiolated shoots. High efficiency transformation system was established. On regeneration medium with 0. 5 mg.l-1 NAA,transgenic plants with GUS gene were Obtained from 400 internode explants after cocultivation with EHA105. The integration of the GUS gene into apple genome was confirmed and Southern blot analysis. The gene expression was confirmed by GUS-histochem-istry staining.

采用根瘤农杆菌(Agrobacterium tumefaciens)强株系EHA105(p35sGUS-intron)研究了影响‘皇家嘎拉’苹果(Mains domestica Borkh.)外植体的β-葡萄糖醛酸酶(GUS)基因的瞬时、稳定表达水平和转基因植株的再生。证明在培养基生长素(IBA、NAA)存在的条件下,外植体的GUS基因的瞬时表达水平提高了3-4倍,而共培养两周后稳定表达水平提高2倍以上,产生9.8个GUS愈伤组织表达区域。白化处理促进外植体的基因转化,白化处理的新梢顶端第一节间外植体GUS表达区域比常用的叶片外植体高4倍。在生长素存在的条件下 2%外植体获得了转基因植株。Southern BlotDNA杂交和组织化学染色分析证明GUS基因已整合到苹果的染色体上,并得以表达。

Transgenic plants regenerated from cotyledons of M sativa L infected using Agrobacterium tumefaciens A281 with plasmid pBF649 containing a gene encoding protein of high sulfur-amino acid content (HNP) were obtained successfully. The plants grew and fertiled well in field. Cotyledon explants were better recipient for transformation of M sativa L. Environment of suitable temperature (15℃) and high humidity on high viability of the plants transplanted into soil were essential conditions.

通过农杆菌介导法将高含硫氨基酸蛋白基因转入苜蓿,成功地诱导转基因植株再生。转化植株生长和发育良好。苜蓿子叶外植体是较理想的转化受体。冷凉湿润的环境条件是苜蓿移栽成活率高所必需的。

 
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