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blast分析
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  blast analysis
     The results of Blast analysis showed that the A11T13Avr3-10-11S157 was homologous with EST G001F10.XT7,and the A2AT19Avr4S125 had the same sequence with EST PH051G10.XT7 in Phytophthora germinating cyst EST library .
     Blast分析表明:A11T13Avr3-10-11S157与马铃薯晚疫病菌正萌发孢子囊(germinating sporangium)EST(expressed sequence tag)库中的序列PG001F10.XT7同源;
     ④Sequencing and Blast analysis.
     ④测序及Blast分析
短句来源
     BLAST analysis showed that the gene sequence obtained was 50% identity to that of Arabidopsis thaliana (thale cress).
     BLAST分析结果表明,该片段与Arabidopsis thaliana (thale cress)的耐酸铝相关基因序列同源性为50%。
短句来源
     BLAST analysis of the sequence in NCBI database showed that it has 86% similarity with the glucanase gene of Bacillus sp. KSM-S237, and the edcoded polypeptide shows 87% similarity with the β-1,4-endo-glucanase of Bacillus sp.KSM-64 at amino acid level.
     BLAST分析结果表明,该基因与Bacillus sp.KSM-S237来源的纤维素酶基因AB018420具86%相似性,所编码的多肽与Bacillus sp.KSM-64来源的β-1,4-内切葡聚糖酶具89%的相似性,故该基因是一新发现的β-1,4-内切葡聚糖酶基因.
短句来源
     Blast analysis showed that HSD45 was identical to INCA1 (Inhibitor of CDK interacting with Cyclin A1), a novel cyclin A1/CDK2 interaction partner in a yeast-triple hybrid approach.
     Blast分析发现HSD45与一新基因完全相同。 该基因因能通过与cyclin A1相互作用抑制CDK的活性,故命名为INCAl(Inhibitor of CDK interacting with Cyclin A1)。
短句来源
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  “blast分析”译为未确定词的双语例句
     Identities of the nucleotide sequences between BmCPV-C and BmCPV-1,DpCPV-1,LdCPV-1,LdCPV-14,TnCPV-15 were 89%,81%,81%,54.1% and 50.9% respectively and that of the amino acid sequences were 96.5%,93.1%,92.9%,41.1% and 33.5% respectively.
     通过在线blast分析,与BmCPV1、DpCPV1、LdCPV1、LdCPV14、TnCPV15核苷酸同源性分别为89%、81%、81%、541%和509%,氨基酸同源性分别为965%、931%、929%、411%和335%。
短句来源
     The ns1 gene of Qa/ST/852/01 (H5N1) isolate was closer to that of some H5N1 strains prevalent in southern China in recent years, the homologies of nucleotide and amino acid sequences were 99.0%~97.5% and 99.1%~97.8%, respectively.
     BLAST分析表明,Qa/ST/852/01(H5N1)病毒株ns1基因与近年来从华南地区分离的禽H5N1毒株的ns1基因有很高的同源性。
短句来源
     Comparison of the fragment sequence with that of Canis familiaris,Bos Taurus and Homo sapiens dopamine receptor D1 gene was carried out through blast in GenBank,the result showed that the homologies of the nucleotide sequence was 99% with Canis familiaris,93% with Bos Taurus and 92% with Homo sapiens.
     Genebank中的Blast分析表明,北极狐多巴胺D1受体基因与家狗(Canis familiaris)的同源性为99%,与牛(Bos taurus)的同源性为93%,与人(Homo sapiens)的同源性为92%。
短句来源
     ③The identified results of restriction mapping,DNA sequencing and sequence alignment analysis by BLAST proved that recombinant pUC19-ADH1C*1 plasmid was constructed successfully.
     ③用限制性酶谱和DNA测序并作BLAST分析证实pUC19-ADH1C*1重组质粒构建成功。
短句来源
     After sequencing, the positive clones were analyzed with BLAST. Four candidate proteins were chosen for further characterization: they are PCBP4 [Homo sapiens poly (rC) binding protein 4], TXNDC9 (Homo sapiens thioredoxin domain containing 9), FTL (Homo sapiens ferritin, light polypeptide) and GRK5 (Homo sapiens G protein-coupled receptor kinase 5).
     阳性克隆测序并将测序结果用BLAST分析,3,44,47,54号阳性克隆的氨基酸序列分别和人PCBP4 [poly(rC) binding protein 4]、TXNDC9 (thioredoxin domain containing 9)、FTL (ferritin, light polypeptide)和GRK5 (G protein-coupled receptor kinase 5) 4种蛋白具有高度的同源性。
短句来源
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  相似匹配句对
     , G.
     分析,G.
短句来源
     C, methylation analysis etc were used.
     分析,G. C.
短句来源
     ④Sequencing and Blast analysis.
     ④测序及Blast分析
短句来源
     Performance analysis of variable rate variable power V-BLAST system
     可变速率可变功率的V-BLAST系统的性能分析
短句来源
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  blast analysis
trichocarpa (AF057708) determined by blast analysis in the GenBank.
      
A BLAST analysis revealed an open reading frame (ORF) that appears to encode for the Tetrahymena DNA-[adenine] methyltransferase ((MTase), based on the presence of motifs characteristic of the enzymes in prokaryotes.
      
In silico analysis indicated that the coding region contains a single intron and translates into an 893 amino acid protein, with BLAST analysis identifying five conserved nitrate reductase domains within the protein.
      
The bacterial strains were identified as Bacillus species by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database.
      
The BLAST analysis demonstrated that the cloned sequence is 100% homologous with sequences reported overseas.
      
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Through screening a human fetal brain cDNA library, a cDNA similar to ganglioside induced gene, was isolated. Northern analysis revealed a 1.1 kb transcript highly expressed in fetal brain presented at lower level in adult brain. The novel ganglioside induced gene was localized to chromosome 10q12 by using radiation hybrid G3 panels. Blast and information of the novel ganglioside induced gene were analysed. The novel ganglioside induced cDNA is 1 163 bp . Bioinformatical analysis of those genes has been...

Through screening a human fetal brain cDNA library, a cDNA similar to ganglioside induced gene, was isolated. Northern analysis revealed a 1.1 kb transcript highly expressed in fetal brain presented at lower level in adult brain. The novel ganglioside induced gene was localized to chromosome 10q12 by using radiation hybrid G3 panels. Blast and information of the novel ganglioside induced gene were analysed. The novel ganglioside induced cDNA is 1 163 bp . Bioinformatical analysis of those genes has been performed. The novel ganglioside induced cDNA was associated with neurotrophic development and regulation of cell cycle and signal transduction. It is interesting and impoptant that the novel cDNA may play a critical role in carcinogenesis. Further analysis the function of novel ganglioside induced cDNA would be helpful to understand the molecular mechanism of malignant carcinoma.

通过构建、筛选人 18周胎脑cDNA文库 ,克隆到一条与神经节苷脂诱导分化相关蛋白高度同源的新基因 .经HUGO/GDB人类基因命名委员会的同意命名为GDAP1L1.进行新基因的全序列测定、RH定位分析、Blast分析及生物学信息分析 .Northern杂交提示GDAP1L1基因在胎脑中高度表达 ,但在成人脑组织中低表达 .新的神经节苷脂诱导分化相关蛋白基因的表达和功能研究初步提示 :全长新神经节苷脂诱导分化相关基因核苷酸序列长 116 3bp ,RH定位分析新基因定位在染色体 2 0 q12区 ,BLASTN ,BLASTP ,TBLASTN分析新基因的蛋白质序列与人和鼠“神经节苷脂诱导分化相关蛋白 1”有 5 8%的同源性 ,而与人的另一条“类似神经节苷脂诱导分化相关蛋白 1”的部分蛋白序列 (4 7~ 2 5 3aa)的同源性达 10 0 % ,新基因蛋白在 3′ ,5′端分别多出 46aa和 114aa的长度 ,生物学信息分析证实神经节苷脂诱导分化相关基因与神经营养及与细胞凋亡有密切关系 ,全长新神经节苷脂诱导分化相关基因是一个与神经营养与发育及细胞周期调控、信号传导有关的基因 ,可能在肿...

通过构建、筛选人 18周胎脑cDNA文库 ,克隆到一条与神经节苷脂诱导分化相关蛋白高度同源的新基因 .经HUGO/GDB人类基因命名委员会的同意命名为GDAP1L1.进行新基因的全序列测定、RH定位分析、Blast分析及生物学信息分析 .Northern杂交提示GDAP1L1基因在胎脑中高度表达 ,但在成人脑组织中低表达 .新的神经节苷脂诱导分化相关蛋白基因的表达和功能研究初步提示 :全长新神经节苷脂诱导分化相关基因核苷酸序列长 116 3bp ,RH定位分析新基因定位在染色体 2 0 q12区 ,BLASTN ,BLASTP ,TBLASTN分析新基因的蛋白质序列与人和鼠“神经节苷脂诱导分化相关蛋白 1”有 5 8%的同源性 ,而与人的另一条“类似神经节苷脂诱导分化相关蛋白 1”的部分蛋白序列 (4 7~ 2 5 3aa)的同源性达 10 0 % ,新基因蛋白在 3′ ,5′端分别多出 46aa和 114aa的长度 ,生物学信息分析证实神经节苷脂诱导分化相关基因与神经营养及与细胞凋亡有密切关系 ,全长新神经节苷脂诱导分化相关基因是一个与神经营养与发育及细胞周期调控、信号传导有关的基因 ,可能在肿瘤的发生中具有重要作用 ,其功能的进一步研究将为肿瘤机理的阐明提供思路 .

In order to study the neutrophil gelatinase assiciated lipocalin(NGAL)gene expression character in the progress of malignant transformation of human immortalized esophageal epithelial cell, differentially expressed NGAL gene was identified by using cDNA microarray in the human immortalized esophageal epithelial cell line(SHEE) and malignant transformed esophageal cancer cell line(SHEEC). NGAL expression profile was further confirmed by Northern blot and RT PCR. A cDNA encoding NGAL from SHEEC was amplified...

In order to study the neutrophil gelatinase assiciated lipocalin(NGAL)gene expression character in the progress of malignant transformation of human immortalized esophageal epithelial cell, differentially expressed NGAL gene was identified by using cDNA microarray in the human immortalized esophageal epithelial cell line(SHEE) and malignant transformed esophageal cancer cell line(SHEEC). NGAL expression profile was further confirmed by Northern blot and RT PCR. A cDNA encoding NGAL from SHEEC was amplified by PCR and sequenced. Alignment was analyzed by NCBI database. The results indicated that NGAL gene was overexpressed in the SHEEC. The coding region cDNA of NGAL from SHEEC was cloned and identified. Alignment of its deduced amino acid sequence compared to the mouse 24p3 protein, the rat neu related lipocalin(NRL), the human NGAL from neutrophil and ovarian cancer demonstrated a very high degree of conservation. It can be concluded that NGAL overexpression possibly played an important role in the progress of malignant transformation of human immortalized esophageal epithelial cell. NGAL may be a new oncogene or promoter tumor gene.

为研究NGAL (neutrophilgelatinase associatedlipocalin)基因在永生化食管上皮细胞恶性转化中的表达情况 ,以永生化食管上皮细胞系SHEE和食管癌细胞系SHEEC互为对照 ,用cDNA微列阵进行筛选 ,用RNA印迹和RT PCR进行鉴定 ,cDNA克隆测序后与GenBank进行BLAST分析比较 .结果表明NGAL基因在SHEEC中出现显著差异过表达 ,其cDNA序列与小鼠 2 4p3、大鼠NRL (neu relatedlipocalin)、人中性粒细胞NGAL和卵巢癌NGAL具有较高的相似性 .这提示NGAL基因在永生化食管上皮细胞恶性转化中可能发挥着重要作用 ,可能是一种新的癌基因或促癌基因

Objective Through isolating the histidine kin ase gene of Aspergillus fumigatus,to detect its role in the invasive aspergillosis.Methods Using degenerate primers for highly conserved regions of his-tidine kinase,RT -PCR was performed with cDNA of Aspergillus fumigatus as a template.The gene expression of it in vitro and in vivo was investig ated by Northern blot.Results A fragment of this gene was cloned fro m Aspergillus fumigatus that is highly homologous to tesA gene of Aspergillus nidulans,which was expressed...

Objective Through isolating the histidine kin ase gene of Aspergillus fumigatus,to detect its role in the invasive aspergillosis.Methods Using degenerate primers for highly conserved regions of his-tidine kinase,RT -PCR was performed with cDNA of Aspergillus fumigatus as a template.The gene expression of it in vitro and in vivo was investig ated by Northern blot.Results A fragment of this gene was cloned fro m Aspergillus fumigatus that is highly homologous to tesA gene of Aspergillus nidulans,which was expressed at high level during invasive infection.Conclusion The results indicate that this gene may attribute to the invasive aspergillosis.

目的分离克隆烟曲霉组氨酸激酶基因,进一步探讨其在侵袭性感染中的作用。方法基于组氨酸激酶保守区设计简并引物,通过逆转录-聚合酶链反应获得特异性扩增产物,对目的产物克隆、测序。通过Blast分析进行相似性比较。以获得的目的基因片段作为探针,通过Northern杂交比较该基因在体外与体内感染时表达的差异。结果逆转录-聚合酶链反应获得2个片段,长度分别为171bp、305bp,其中305bp片段与构巢曲霉tesA基因的同源性>80%。该基因在小鼠侵袭性肺曲霉病中表达水平明显增加。结论烟曲霉组氨酸激酶基因的表达可能有助于其在体内存活,该基因可能是烟曲霉潜在的毒力基因。

 
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