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blast分析
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  blast analysis
trichocarpa (AF057708) determined by blast analysis in the GenBank.
      
A BLAST analysis revealed an open reading frame (ORF) that appears to encode for the Tetrahymena DNA-[adenine] methyltransferase ((MTase), based on the presence of motifs characteristic of the enzymes in prokaryotes.
      
In silico analysis indicated that the coding region contains a single intron and translates into an 893 amino acid protein, with BLAST analysis identifying five conserved nitrate reductase domains within the protein.
      
The bacterial strains were identified as Bacillus species by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database.
      
The BLAST analysis demonstrated that the cloned sequence is 100% homologous with sequences reported overseas.
      
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Through screening a human fetal brain cDNA library, a cDNA similar to ganglioside induced gene, was isolated. Northern analysis revealed a 1.1 kb transcript highly expressed in fetal brain presented at lower level in adult brain. The novel ganglioside induced gene was localized to chromosome 10q12 by using radiation hybrid G3 panels. Blast and information of the novel ganglioside induced gene were analysed. The novel ganglioside induced cDNA is 1 163 bp . Bioinformatical analysis of those genes has been...

Through screening a human fetal brain cDNA library, a cDNA similar to ganglioside induced gene, was isolated. Northern analysis revealed a 1.1 kb transcript highly expressed in fetal brain presented at lower level in adult brain. The novel ganglioside induced gene was localized to chromosome 10q12 by using radiation hybrid G3 panels. Blast and information of the novel ganglioside induced gene were analysed. The novel ganglioside induced cDNA is 1 163 bp . Bioinformatical analysis of those genes has been performed. The novel ganglioside induced cDNA was associated with neurotrophic development and regulation of cell cycle and signal transduction. It is interesting and impoptant that the novel cDNA may play a critical role in carcinogenesis. Further analysis the function of novel ganglioside induced cDNA would be helpful to understand the molecular mechanism of malignant carcinoma.

通过构建、筛选人 18周胎脑cDNA文库 ,克隆到一条与神经节苷脂诱导分化相关蛋白高度同源的新基因 .经HUGO/GDB人类基因命名委员会的同意命名为GDAP1L1.进行新基因的全序列测定、RH定位分析、Blast分析及生物学信息分析 .Northern杂交提示GDAP1L1基因在胎脑中高度表达 ,但在成人脑组织中低表达 .新的神经节苷脂诱导分化相关蛋白基因的表达和功能研究初步提示 :全长新神经节苷脂诱导分化相关基因核苷酸序列长 116 3bp ,RH定位分析新基因定位在染色体 2 0 q12区 ,BLASTN ,BLASTP ,TBLASTN分析新基因的蛋白质序列与人和鼠“神经节苷脂诱导分化相关蛋白 1”有 5 8%的同源性 ,而与人的另一条“类似神经节苷脂诱导分化相关蛋白 1”的部分蛋白序列 (4 7~ 2 5 3aa)的同源性达 10 0 % ,新基因蛋白在 3′ ,5′端分别多出 46aa和 114aa的长度 ,生物学信息分析证实神经节苷脂诱导分化相关基因与神经营养及与细胞凋亡有密切关系 ,全长新神经节苷脂诱导分化相关基因是一个与神经营养与发育及细胞周期调控、信号传导有关的基因 ,可能在肿...

通过构建、筛选人 18周胎脑cDNA文库 ,克隆到一条与神经节苷脂诱导分化相关蛋白高度同源的新基因 .经HUGO/GDB人类基因命名委员会的同意命名为GDAP1L1.进行新基因的全序列测定、RH定位分析、Blast分析及生物学信息分析 .Northern杂交提示GDAP1L1基因在胎脑中高度表达 ,但在成人脑组织中低表达 .新的神经节苷脂诱导分化相关蛋白基因的表达和功能研究初步提示 :全长新神经节苷脂诱导分化相关基因核苷酸序列长 116 3bp ,RH定位分析新基因定位在染色体 2 0 q12区 ,BLASTN ,BLASTP ,TBLASTN分析新基因的蛋白质序列与人和鼠“神经节苷脂诱导分化相关蛋白 1”有 5 8%的同源性 ,而与人的另一条“类似神经节苷脂诱导分化相关蛋白 1”的部分蛋白序列 (4 7~ 2 5 3aa)的同源性达 10 0 % ,新基因蛋白在 3′ ,5′端分别多出 46aa和 114aa的长度 ,生物学信息分析证实神经节苷脂诱导分化相关基因与神经营养及与细胞凋亡有密切关系 ,全长新神经节苷脂诱导分化相关基因是一个与神经营养与发育及细胞周期调控、信号传导有关的基因 ,可能在肿瘤的发生中具有重要作用 ,其功能的进一步研究将为肿瘤机理的阐明提供思路 .

In order to study the neutrophil gelatinase assiciated lipocalin(NGAL)gene expression character in the progress of malignant transformation of human immortalized esophageal epithelial cell, differentially expressed NGAL gene was identified by using cDNA microarray in the human immortalized esophageal epithelial cell line(SHEE) and malignant transformed esophageal cancer cell line(SHEEC). NGAL expression profile was further confirmed by Northern blot and RT PCR. A cDNA encoding NGAL from SHEEC was amplified...

In order to study the neutrophil gelatinase assiciated lipocalin(NGAL)gene expression character in the progress of malignant transformation of human immortalized esophageal epithelial cell, differentially expressed NGAL gene was identified by using cDNA microarray in the human immortalized esophageal epithelial cell line(SHEE) and malignant transformed esophageal cancer cell line(SHEEC). NGAL expression profile was further confirmed by Northern blot and RT PCR. A cDNA encoding NGAL from SHEEC was amplified by PCR and sequenced. Alignment was analyzed by NCBI database. The results indicated that NGAL gene was overexpressed in the SHEEC. The coding region cDNA of NGAL from SHEEC was cloned and identified. Alignment of its deduced amino acid sequence compared to the mouse 24p3 protein, the rat neu related lipocalin(NRL), the human NGAL from neutrophil and ovarian cancer demonstrated a very high degree of conservation. It can be concluded that NGAL overexpression possibly played an important role in the progress of malignant transformation of human immortalized esophageal epithelial cell. NGAL may be a new oncogene or promoter tumor gene.

为研究NGAL (neutrophilgelatinase associatedlipocalin)基因在永生化食管上皮细胞恶性转化中的表达情况 ,以永生化食管上皮细胞系SHEE和食管癌细胞系SHEEC互为对照 ,用cDNA微列阵进行筛选 ,用RNA印迹和RT PCR进行鉴定 ,cDNA克隆测序后与GenBank进行BLAST分析比较 .结果表明NGAL基因在SHEEC中出现显著差异过表达 ,其cDNA序列与小鼠 2 4p3、大鼠NRL (neu relatedlipocalin)、人中性粒细胞NGAL和卵巢癌NGAL具有较高的相似性 .这提示NGAL基因在永生化食管上皮细胞恶性转化中可能发挥着重要作用 ,可能是一种新的癌基因或促癌基因

Objectives: To clone the male infertility and spermatogenesis related genes by using differential display technique. Methods: Applying mRNA differential display technique,we analyzed mRNA different expression of three kinds of testis tissues (Sertoli cell only syndrome,spermatogenic arrest and controller). 17 fragments expressed in both spermatogenic arrest and controller but not in Sertoli cell only syndrome were found and all of these fragments were cloned and sequenced.The sequences were compared...

Objectives: To clone the male infertility and spermatogenesis related genes by using differential display technique. Methods: Applying mRNA differential display technique,we analyzed mRNA different expression of three kinds of testis tissues (Sertoli cell only syndrome,spermatogenic arrest and controller). 17 fragments expressed in both spermatogenic arrest and controller but not in Sertoli cell only syndrome were found and all of these fragments were cloned and sequenced.The sequences were compared with the data in GenBank with software Blast 2.0. Results : 3 new ESTs were cloned and shown no homology in GenBank. Conclusions: DDRT PCR is a useful tool to identify new spermatogenesis related genes.These ESTs may be ESTs of some new spermatogenesis related genes or be new ESTs of some genes. Ntl J Androl,2001,7(1):20~23

目的 :通过差异显示技术克隆精子发生相关基因 ,并对这些基因进行初步的研究。 方法 :应用mRNA差异显示技术 ,对唯支持细胞综合征病人、生精阻断在精原细胞的病人和正常人的睾丸组织进行基因表达图谱分析 ,获得在唯支持细胞综合征病人的睾丸组织中表达缺失、生精阻断在精原细胞病人和正常人中表达的一些差异片段 ,并对这些片段进行克隆和测序 ,同时与GenBank中的EST数据进行BLAST分析。 结果 :通过DDRT PCR获得了 17个不同的差异表达的EST片段 ,同时与GenBank中的数据进行BLAST分析 ,发现其中有 3个片段未见有同源性。 结论 :DDRT PCR在克隆精子发生相关基因研究中具有可行性 ,获得的 3个EST可能是与精子发生有关的新基因的EST或者某些基因的新的EST。

 
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