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   blast分析 在 农作物 分类中 的翻译结果: 查询用时:0.524秒
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blast分析
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  blast analysis
trichocarpa (AF057708) determined by blast analysis in the GenBank.
      
A BLAST analysis revealed an open reading frame (ORF) that appears to encode for the Tetrahymena DNA-[adenine] methyltransferase ((MTase), based on the presence of motifs characteristic of the enzymes in prokaryotes.
      
In silico analysis indicated that the coding region contains a single intron and translates into an 893 amino acid protein, with BLAST analysis identifying five conserved nitrate reductase domains within the protein.
      
The bacterial strains were identified as Bacillus species by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database.
      
The BLAST analysis demonstrated that the cloned sequence is 100% homologous with sequences reported overseas.
      
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Mitochorndrial DNA (mtDNA),nuclear DNA (nDNA), and genomic DNA (gDNA) were individually extracted from Zhenshan 97A, Xieqingzao A and A 23, which were cytoplasmic male sterile (wild abortive, dwarf abortive and BT), and from Zhenshan 97B, Xieqingzao B and BT, which were maintainers lines. Each of them was analyzed with 500 random primers by RAPD. Six fragments specific to male sterile lines were amplified. A fragment, PWH 17, associated with wild and dwarf abortive type cytoplasmic male sterility was analyzed...

Mitochorndrial DNA (mtDNA),nuclear DNA (nDNA), and genomic DNA (gDNA) were individually extracted from Zhenshan 97A, Xieqingzao A and A 23, which were cytoplasmic male sterile (wild abortive, dwarf abortive and BT), and from Zhenshan 97B, Xieqingzao B and BT, which were maintainers lines. Each of them was analyzed with 500 random primers by RAPD. Six fragments specific to male sterile lines were amplified. A fragment, PWH 17, associated with wild and dwarf abortive type cytoplasmic male sterility was analyzed by Southern blotting and sequencing, and also tested by SCAR. It was 1879bp in length, which contained 6 open reading frames and 8 repeated sequences. BLAST search revealed that partial sequence in PWH 17 showed high similarity to the upstream sequence of tRNA Asp gene in Elytrigia elongate (GenBank accession number U14335) and wheat (X13243). The similarity ratios were 97% and 100%. It was inferred that the possible position of PWH 17 in rice (wild abortive) could be located between tRNA Asp and Cox Ⅱ, which will be discussed further.

该项研究利用RAPD技术 ,对野败型、矮败型和BT型细胞质雄性不育系、保持系 ,基因组DNA进行PCR扩增 ,分离到 6条不育系特有的扩增片段 ,并对野败型、矮败型不育系共有的片段进行了Southern分析、DNA序列分析和SCAR验证 ,该片段全长 1879bp ,包含 6个开放阅读框架和 8对重复序列。BLAST分析表明 ,该片段部分区域与Elytrigiaelongata、小麦线粒体tRNA Asp基因上游一段序列同源。并对该片段在线粒体DNA中的可能位置等进行了讨论

Two degenerate primers were designed according to the conserved region among the known plant cystatins.A cDNA fragment of 204 bp was amplified by RT-PCR (reverse transcription polymerase chain reaction) of total RNA extracted from fresh leaves of Tea plant (Camellia sinensis cv Longjing43).A full-length cDNA of the cystatin gene was obtained by 3'/5'RACE (rapid amplification of cDNA ends).The cDNA sequence of this 627 bp clone contained an open reading frame encoding a polypeptide of 101 amino acid residues...

Two degenerate primers were designed according to the conserved region among the known plant cystatins.A cDNA fragment of 204 bp was amplified by RT-PCR (reverse transcription polymerase chain reaction) of total RNA extracted from fresh leaves of Tea plant (Camellia sinensis cv Longjing43).A full-length cDNA of the cystatin gene was obtained by 3'/5'RACE (rapid amplification of cDNA ends).The cDNA sequence of this 627 bp clone contained an open reading frame encoding a polypeptide of 101 amino acid residues with a predicable molecular mass of 11.026 KDa.The deduced amino acid sequence contained the motif QXVXG conserved among most members of the cystatin superfamily.By using the program of Blast on GenBank database,the sequence presented a high match with the cystatin genes from other plants,such as European chestnut,Cassava,Cowpea,Tomato,Soybean et al.All researched out sequences were all cystatins,so we can conclude that the cloned sequence is a member of cystatin gene from Tea plant.

对多种已知植物巯基蛋白酶抑制剂(cystatin)基因的氨基酸序列进行比对分析,根据其高度保守的氨基酸序列设计一对简并引物,并从茶树品种龙井43鲜叶中提取总RNA,用RT-PCR法扩增出一204bp的cDNA特异片段,然后通过3’/5’RACE的方法,分别扩增出3’端和5’端的序列,从而获得茶树巯基蛋白酶抑制剂基因的cDNA全长序列,所得序列全长627bp,编码101个氨基酸,分子量约11.062KDa。该基因在推测的氨基酸序列中含有巯基蛋白酶抑制剂家族中高度保守的、与其活性有关的QXVXG结构,且经Blast分析表明,该基因序列与其他植物巯基蛋白酶抑制剂基因的氨基酸序列同源性为54%~77%。

Soybean is one of world's most important oil crops. China is the origin centre of soybean, has a rich source of soybean germplasms. Soybean Cyst Nematode (SCN) (Heterodera glycines Ichinohe) is a kind of soilborn sedentary endoparasite nematode and the pathogen of soybean chlorsis, etc., is difficult to manage. It has over ten physiological races. It is economically the most destructive pathogen affecting soybean culture. SCN Race 3?4 are the predominant races that are the most damaging pathogens in China. The...

Soybean is one of world's most important oil crops. China is the origin centre of soybean, has a rich source of soybean germplasms. Soybean Cyst Nematode (SCN) (Heterodera glycines Ichinohe) is a kind of soilborn sedentary endoparasite nematode and the pathogen of soybean chlorsis, etc., is difficult to manage. It has over ten physiological races. It is economically the most destructive pathogen affecting soybean culture. SCN Race 3?4 are the predominant races that are the most damaging pathogens in China. The study on resistance to SCN is always one of hot-spots in the world's soybean resistant-disease breeding. In the former study, according to the conserved sequence of plant resistant genes to nematode cloned, a specific band was amplified in nematode resistant varieties identified conventionally to SCN race3, while nothing in susceptible varieties identified conventionally to SCN race3(total 15 soybean cultivars). Based on former study, the genomic DNA of Soybean cultivar Peking (highly resistant to cyst nematode race3) was amplified and the amplified fragment, named RSCN3, was cloned and sequenced. The results of blast showed that DNA sequence had more than 80% identity with Glycine max receptor-like kinase RHG4 gene (Rhg4), O.sativa TMK (leucine rich protein, receptor-like kinase) gene, etc., respectively. The putative amino acid according to RSCN3 DNA sequence had 21 leucines and high identity with receptor kinase in plant and leucine rich repeat protein kinase. It was supposed that the RSCN3 clone might be a RGA of the related disease-resistant gene with the likely role of receptor kinase. At last, direct submitting sequence data to GenBank, GenBank accession number for the nucleotide sequence is AY580161.

大豆是主要的油料作物,起源于中国,在我国种质资源十分丰富。大豆孢囊线虫(SCN)(HeteroderoglycinesIchinohe)是一种土传的定居性内寄生线虫,不易防治,常引起大豆黄萎病等病害,是大豆生产上危害最大的病害之一。大豆孢囊线虫病生理小种多达十几种,在我国,大豆孢囊线虫病病原主要为3、4号生理小种。大豆抗孢囊线虫的研究一直是世界上大豆抗病育种研究的热点之一。在本课题的前期研究中,根据已克隆的植物抗孢囊线虫病基因的保守序列设计引物,对经常规鉴定为抗(感)孢囊线虫3号生理小种的15个大豆品种基因组DNA进行PCR扩增,在大豆抗病品种中获得一条大豆抗孢囊线虫的特异条带。本研究在此基础上利用该对引物,对高抗孢囊线虫3号小种的北京小黑豆基因组DNA进行扩增,并克隆了特异扩增片段,命名为RSCN3,经测序及BLAST分析,发现其DNA序列与GenBank、EMBL、DDBJ、PDB中的大豆似受体激酶RHG4、水稻TMK(leucinerichprotein,receptor-likekinase)基因等均有80%以上的同源性。根据该DNA序列推测其氨基酸序列,在其序列中共找到21个亮氨酸,将该...

大豆是主要的油料作物,起源于中国,在我国种质资源十分丰富。大豆孢囊线虫(SCN)(HeteroderoglycinesIchinohe)是一种土传的定居性内寄生线虫,不易防治,常引起大豆黄萎病等病害,是大豆生产上危害最大的病害之一。大豆孢囊线虫病生理小种多达十几种,在我国,大豆孢囊线虫病病原主要为3、4号生理小种。大豆抗孢囊线虫的研究一直是世界上大豆抗病育种研究的热点之一。在本课题的前期研究中,根据已克隆的植物抗孢囊线虫病基因的保守序列设计引物,对经常规鉴定为抗(感)孢囊线虫3号生理小种的15个大豆品种基因组DNA进行PCR扩增,在大豆抗病品种中获得一条大豆抗孢囊线虫的特异条带。本研究在此基础上利用该对引物,对高抗孢囊线虫3号小种的北京小黑豆基因组DNA进行扩增,并克隆了特异扩增片段,命名为RSCN3,经测序及BLAST分析,发现其DNA序列与GenBank、EMBL、DDBJ、PDB中的大豆似受体激酶RHG4、水稻TMK(leucinerichprotein,receptor-likekinase)基因等均有80%以上的同源性。根据该DNA序列推测其氨基酸序列,在其序列中共找到21个亮氨酸,将该序列与蛋白质序列同源性进行比较,结果发现与植物中的受体激酶、富含亮氨酸重复的蛋白激酶有较高的同源性。因此推测RSCN3克隆片断为一个与受体激酶有类似作用的抗病相关基因的RGA,并将该序列登录到GenBank中,登录号为:AY580161。

 
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