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细胞增殖实验
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  cell proliferative assay
     Methods: Eukaryotic GM-CSF expressing plasmid was transfected into RMA cells by electroporation. After screening by G418 and cloning by limited dilution, a relative GM-CSF high expressing cell clone was selected by RT-PCR, hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay.
     方法 :将小鼠GM CSF真核表达质粒用电穿孔法导入小鼠淋巴瘤细胞系RMA ,有限稀释法制备单个细胞克隆 ,经RT PCR、骨髓祖细胞增殖实验和集落形成实验筛选相对高表达GM CSF的RMA克隆 ,并观察其生物学特性的改变。
短句来源
     The constructed vector was transfected into RMA cells by electroporation. After screening by G418 and cloning by limiting dilution, a relative high GM-CSF expressing cell clone was selected by RT-PCR, hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay. The cells of this clone were inactivated by mitomycin-C and vaccinated mice to induce antitumor immune reaction.
     用电穿孔法将构建的载体导入小鼠淋巴瘤细胞系RMA,有限稀释法制备单个细胞克隆,经RT-PCR、骨髓祖细胞增殖实验和集落形成实验筛选相对高表达GM-CSF的RMA克隆,该克隆细胞经丝裂霉素灭活后免疫小鼠以诱导其产生抵抗RMA肿瘤细胞再攻击的能力。
短句来源
     The biological activity was confirmed by the hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay.
     骨髓造血祖细胞增殖实验和骨髓造血祖细胞集落刺激实验鉴定其生物学活性。
短句来源
     Method The eukaryotic GM-CSF expressing plasmid was transfected into C57 mice T lymphoma cell by electroporation. A relative GM-CSF high expressing cell clone was selected by RT-PCR,haemopoietic stem cell proliferative assay and hematopoietic progenitor cell colony for mation assay after screeing by G-418 and cloning by coubling dilution.
     方法:将小鼠GM-CSF真核表达质粒用电穿孔方法导入C57小鼠T淋巴瘤细胞中,倍比稀释法制备单个细胞克隆,经RT-PCR、骨髓干细胞增殖实验和集落形成实验筛选相对高表达GM-CSF的RMA-GM克隆。
短句来源
  cell proliferation assay
     Methods CD4~+ CD25~+ regulatory T cells were isolated by MACS(magnetic cell sorting)from naive BALB/ c mice. Their suppression on the proliferation of CD4~+ CD25~- T cells was analyzed by cell proliferation assay.
     方法用MACS(magnetic cell sorting)从BALB/c小鼠静息T细胞分离纯化CD4~+ CD25~+ T细胞,体外细胞增殖实验观察其对CD4~+ CD25~- T细胞的免疫抑制作用;
短句来源
     Methods:Human cholangicarcinoma cell line(HUCCT1) was cultured and treated with PGE2, and the cell growth rate and viability was measured by cell proliferation assay with WST-1 regent.
     方法:体外培养胆管上皮癌细胞株(HuCCT1),给予外源PGE2处理,以WST-1细胞增殖实验检测肿瘤细胞增殖率;
短句来源
     Methods: The inhibition of the lung cancer cells, binding with tumor cells, isotypes, and inhibition of implanted tumor growth of 1E2 monoclonal antibody were studied by immunofluorescence, ELISA, cell proliferation assay, and in vivo tumor treatment experiments. The corresponding antigen of 1E2 was identified by Western blotting and MALDI-TOF mass spectrometry.
     方法:采用活细胞荧光、MTT细胞增殖实验、ELISA、动物体内治疗实验等方法检测鼠单克隆抗体1E2对人肺癌细胞增殖的抑制、单抗与癌细胞的结合部位、单抗的亚类和单抗对肺癌移植瘤的抑制,以Western blotting和MALDI TOF质谱方法鉴定该功能性单抗的抗原。
短句来源
     T cell response to 23 polypeptides synthesized based on nucleocapsid protein was examined by IFN-γ ELISPOT and T cell proliferation assay in 7 patients with hemorrhagic fever with renal syndrome (HFRS) in the convalescent phase.
     用IFN γELISPOT实验和T细胞增殖实验 ,测试 7名患者PBMC对 2 3条NPC 端合成多肽的T细胞应答。
短句来源
     The purity was examined by SDS PAGE, ELISA and cell proliferation assay were used to determine the activities of fusion protein.
     L- 1尿素溶解包涵体后直接稀释复性 ,SDS- PAGE分析蛋白纯度 ,EL ISA分析技术和细胞增殖实验测定融合蛋白的抗体和细胞因子活性。
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  cell viability assay
     The effects of UV-inactivated EBV(UV-EBV) and IFN-α on cells bearing different types of EBV receptors were determined by colorimetric cell viability assay (MTT).
     本文采用MTT法细胞增殖实验,以纯化并经灭活的EBV刺激具有不同类型EBV受体的细胞。
短句来源
     BVLMP gene transfection, radioimmunobinding assay(RIA) and colorimetric cell viability assay(MTT) were used to investigate the effect and relationship of EBVLMP and EGF autocrine on the growth and proliferation of well differentiated nasopharyngeal carcinoma cell line(CNE1).
     以LMP基因转染CNE1细胞为对象,用放射免疫分析(RIA)和细胞增殖实验(MTT)等方法,研究EB病毒LMP和EGF自分泌在高分化鼻咽癌细胞株CNE1生长增殖中的作用和关系。 结果示:从细胞培养液中检获EGF;
短句来源
  “细胞增殖实验”译为未确定词的双语例句
     5 HT significantly enhanced protein and DNA synthesis in ventricular cells and increased the proliferation of fibroblast at 1 μmol·L -1 ,10μmol·L -1 and 100μmol·L -1 ,with the most effective dose of 10μmol·L -1 . Norepinephrine seems more potent than 5 HT in protein and DNA synthesis.
     细胞增殖实验发现 5 HT在 1μmol·L-1、10 μmol·L-1和 10 0μmol·L-1时均显著促进心肌细胞蛋白质和DNA合成及心脏成纤维细胞增殖 ,以 10 μmol·L-1剂量时作用最强 ,但作用强度弱于同浓度去甲肾上腺素。
短句来源
     RESULTS:① It was shown that MCP 1 enhanced the proliferation of rat BMMSCs slightly in vitro in the manner of concentration dependence with the concentration gradient of 0,5,25,50,75 and 150 μ g/L.
     结果:①二甲基偶氮唑盐法测定细胞增殖实验结果:在体外,单核细胞趋化蛋白1以浓度梯度(0,5,25,50,75,150μg/L)依赖方式促进大鼠骨髓间充质干细胞的增殖,但作用较温和。
短句来源
     The transcription and expression of hFL in the transfected COS 7 cells were assayed by RT PCR,ELISA and the experiment of the human umbilical blood CD34 + cell multiplication,respectively,at 72 hours after transfection.
     脂质体介导法将其转染COS 7细胞 ,72h以RT PCR检测转染细胞中外源hFL基因的转录、ELISA法及脐血CD34 + 细胞增殖实验测定转染细胞上清中hFL的含量和活性。
短句来源
     The biological activity of IZrhsCD40L was increased by 10fold compared with that of rhsCD40L on proliferation of B cells and apoptosis of XG2 cell line.
     B细胞增殖实验和人骨髓瘤细胞株XG2凋亡实验结果都表明,异亮氨酸拉链结构提高了rhsCD40L的生物学活性.
短句来源
     Method: 1. Effect of hedyotis diffusa extract on the growth of SPC-A-1 cell line was measured by means of SPC-A-1 cell proliferation experiments.
     方法:1.细胞增殖实验:测定细胞吸光值,观察白花蛇舌草提取液对SPC-A-1 细胞增殖的影响。
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  cell proliferative assay
Furthermore, supernatants from CsA-treated EC, that had been kept for 6 days in culture medium, were able to inhibit the T-cell proliferative assay.
      
The T-cell proliferative assay was not performed for patient 1, whereas patient 4 was anergic and did not even respond to PHA.
      
We are confident that the results of the MCF-7 cell proliferative assay and the binding assay of styrene oligomers to hER in our paper are accurate.
      
  cell proliferation assay
And their suppression on the proliferation of CD4+CD25- T effector cells was analyzed by cell proliferation assay in vitro.
      
The biologically active conformation of purified BbST was confirmed by its efficient growth promoting activity in Nb2 cell proliferation assay.
      
MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors(TRs) with minimum toxicity to the selected cells.
      
The cell proliferation assay revealed that the proliferation promotion activity of HGF complexed with the acidic gelatin was detected, although it was lower than that of original HGF.
      
Cell proliferation assay and soft agar colony formation assay were used to determine the status of the cells proliferation.
      
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  cell viability assay
The bioactivity of diallyl disulfide was evaluated by cell viability assay on HepG2 hepatoma cells.
      
Methods: Cytotoxic effects were measured by use of a cell viability assay.
      
In the present study, the apoptotic effect of cordycepin on OEC-M1, a human oral squamous cancer cell line, was investigated by morphological observations, cell viability assay, annexin V-FITC analysis and flow cytometry methods.
      
In cell viability assay, cell surviving rate significantly decreased as the dosage and duration of cordycepin treatment increased (P?>amp;lt;?0.05).
      
We found that ECG dose-dependently inhibited UVA-induced keratinocyte death as determined by cell viability assay.
      
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The experiments on toxicity of MT-4 cell and restraining proliferation ofMT-4 cell and anti-HIV-1 activity with tungstophosphorate, tungstosilicate, molybdophosphorate and molybdosilicate heteropoly blue compounds of rear earth elementswith keggin structure were studied. The results show that heteropoly blue compounds ofrear earth elements with keggin structure are effective restraining agents with lowtoxcity of cell. In which Ze heteropoly blue compounds of tungstosilicate and tungstophosphorate possess high...

The experiments on toxicity of MT-4 cell and restraining proliferation ofMT-4 cell and anti-HIV-1 activity with tungstophosphorate, tungstosilicate, molybdophosphorate and molybdosilicate heteropoly blue compounds of rear earth elementswith keggin structure were studied. The results show that heteropoly blue compounds ofrear earth elements with keggin structure are effective restraining agents with lowtoxcity of cell. In which Ze heteropoly blue compounds of tungstosilicate and tungstophosphorate possess high restraining activity on CPE caused by HIV--l. The half rastrainingconcentration to CPE (EC50) is less than that of PM-19.

对钨磷、钨硅、铝磷、钼硅四种Keggin结构杂多蓝进行了细胞毒性实验.抑制MT-4细胞增殖实验及抗艾滋病病毒活性实验.结果表明:Keggin结构杂多蓝配合物具有较低的细胞毒性及较强的抗艾滋病病毒活性,其中钨硅和钨磷两电子杂多蓝对由艾滋病病毒引起的细胞病变作用(CPE)具有较强的抑制活性,对CPE的半数抑制浓度(EC50)明显低于pM-19.

To study the regulatory effects of forskolin, an activator of adenylate cyclase, on the expression of 1,25(OH)2 D3 receptor(VDR) mRNA and responsiveness in a human megakaryoblastic leukemia cell line, HIMeg. Methods: VDR mRNA expression was determined by quantitative reverse transcription-polymerase chain reaction(RT-PCR) and cell growth experiments were conducted in liquid culture system and methylcellulose culture system. Results: It was found that forskolin could significantly up-regulate VDR mRNA expression...

To study the regulatory effects of forskolin, an activator of adenylate cyclase, on the expression of 1,25(OH)2 D3 receptor(VDR) mRNA and responsiveness in a human megakaryoblastic leukemia cell line, HIMeg. Methods: VDR mRNA expression was determined by quantitative reverse transcription-polymerase chain reaction(RT-PCR) and cell growth experiments were conducted in liquid culture system and methylcellulose culture system. Results: It was found that forskolin could significantly up-regulate VDR mRNA expression in HIMeg cells in a dose-dependent manner. Consistent with the functionality of the VDR in other target cells, we found that the up-regulation of VDR expression in HIMeg cells by forskolin was accompanied by an increased responsiveness of HIMeg cells to 1,25(OH)2 D3, even though forskolin alone had no effects. Exposure to 1,25(OH)2 D3 in combination with forskolin resulted in a much more significant inhibition of cell proliferation than to 1,25(OH)2 D3 alone. Similarly, forskolin could also augment the differentiation induced by 1,25(OH)2 D3, reflected by a more evident morphological change and a higher percentage of development of cells with multilobular nuclei. These alterations were accompanied by a loss of clonogenic capacity and a decrease in the number of cells in the S phase. Conclusions: VDR mRNA expression in HIMeg cells could be up-regulated by forskolin, an activator of adenylate cyclase, and the heterologous regulation of VDR mRNA expression in HIMeg cells is of biological significance.

目的:研究腺苷酸环化酶激活剂forskolin(F)对1,25-二羟维生素D3[1,25(OH)2D3]受体(VDR)mRNA表达的调节作用及其可能的生物学意义。方法:以人原始巨核白血病细胞系(HIMeg)为模型,VDRmRNA的检测采用定量逆转录-聚合酶链反应;细胞增殖实验采用悬浮培养系统及甲基纤维素培养系统。结果:F对HIMeg细胞VDRmRNA的表达具有向上调节作用,且有明显的时间依赖性特点;F本身虽然对HIMeg细胞的增殖及分化过程无明显影响,但却可以显著地加强1,25(OH)2D3对该细胞系的作用,具体表现在:当F同时存在时,1,25(OH)2D3对HIMeg细胞的增殖过程和克隆形成率的抑制作用更为明显,对HIMeg细胞分化过程的诱导作用亦更为显著;同时,1,25(OH)2D3对HIMeg细胞周期分布的影响也更加突出。结论:F对HIMeg细胞VDRmRNA具有向上调节作用,并伴有1,25(OH)2D3生物学效应的提高,说明下对HIMeg细胞VDRmRNA的向上调节作用具有生物学意义。

Epidermal growth factor (EGF) is a polypeptide important to the proliferation and differentiation of either normal cells or cancer cells, through binding with their receptors (EGF R) on these cells. The study is designed to investigate relationships between the expression of EGF R on gastroenteric cancer cells (SGC7901, KATOⅢ, MKN45, SMMC7721, HT29) and the proliferation of these cells by using receptor radiobinding assay. The results showed 125 I EGF, which specific activity was 6234.5 MBq/mg and bioactive...

Epidermal growth factor (EGF) is a polypeptide important to the proliferation and differentiation of either normal cells or cancer cells, through binding with their receptors (EGF R) on these cells. The study is designed to investigate relationships between the expression of EGF R on gastroenteric cancer cells (SGC7901, KATOⅢ, MKN45, SMMC7721, HT29) and the proliferation of these cells by using receptor radiobinding assay. The results showed 125 I EGF, which specific activity was 6234.5 MBq/mg and bioactive fraction analysed by Lindmo method was 71%, could bind to SGC 7901 more easily and rapidly but not steadily at 37℃ than at 4℃. The numbers of EGF R on these cells were (1.23±0.46)×10 5, (2.13±0.61)×10 5, (1.5±1.47)×10 4, (1.34±0.15)×10 5, (5.68±0.58)×10 4 sites/cell and the dissociation constants were (2.61±0.30)×10 -9 , (8.01±2.01)×10 -9 , (3.52±0.51)×10 -9 , (8.53±1.11)×10 -10 and (8.75±3.84)×10 -9 mol/L, respectively. Interestingly, EGF could stimulate the growth of these cells. Its stimulative effects varied with the types of tumor cell and were correlated with the expression of EGF R on these cells. These results suggest that the expression of EGF R plays an important role in the proliferation of these cells.

为研究表皮生长因子(EGF)受体功能性表达在消化系肿瘤细胞生长调节上的意义,建立了EGF受体放射分析法.Lindmo生物活性分数分析显示自标记125I-EGF具有高的生物活性,温度、时间效应分析确立4℃,3h为最佳受体分析条件.一组消化系肿瘤细胞SGC7901,KATOⅢ,MKN45,SMMC7721,HT29的EGF受体数目分别为(1.23±0.46)×105,(2.13±0.61)×105,(1.5±1.47)×104,(1.34±0.15)×105,(5.68±0.58)×104个/细胞;其解离常数分别为(2.61±0.30)×10-9,(8.01±2.01)×10-9,(3.52±0.51)×10-9,(8.53±1.11)×10-10,(8.75±3.84)×10-9mol/L.细胞增殖实验显示EGF对消化系肿瘤细胞生长具有促进作用,促进作用的强弱与其受体的数目一致.结果提示消化系肿瘤细胞EGF受体功能表达水平与肿瘤生长相关.

 
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