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重组il
相关语句
  recombinant interleukin
     4) As one of effect factors, human recombinant interleukin 1β(IL-1β) was added to Leydig cell culture and measure method was the same.
     4 人重组IL-1β作为单独的影响因素,加入Leydig 细胞内,测量方法同上。
短句来源
     Conclusions Two novel recombinant interleukin6-Pseudomonas exotoxin fusion proteins IL6D24-PE40KDEL and IL6D24-Linker-PE40KDEL have been successfully constructed and they can selectively kill the leukemic cells expressing highly IL6R.
     结论 成功构建了具有靶向杀伤高表达IL6R白血病细胞的新型重组IL6D2 4 PE4 0KDEL融合蛋白 ,为深入地探索利用IL6 /IL6R系统介导靶向治疗高表达IL6R白血病奠定了坚实的基础。
短句来源
     Methods The levels of IFN γ, IL 4 in peripheral blood mononuclear cells (PBMC) before and after receiving recombinant interleukin 10 stimulation were determined by using ELISA in 10 children with chronic lymphocytic thyroiditis (CLT).
     方法 采用酶联免疫吸附法 ,对加入基因重组IL 10处理前后 10例CLT患儿外周血单个核细胞 (PBMC)诱生IFN γ和IL 4水平的变化进行观察。 结果 患儿PBMC产生IFN γ为(30 81± 182 3)ng/L ,IL 4为 (2 89± 32 )ng/L ,IFN γ/IL 4比值为 10 .1± 5 .4;
短句来源
     Recently we have reported that a full cDNA encoding for human interleu- kin-6 receptor(IL6R)has been transfered and stably expressed in LT12 cells having nodetective level of IL6R,a subline from BNML rat acute promyelocytic leukemia model inorder to investigate the selective kill effect of recombinant interleukin6-pseudomonas exo- toxin fusion proteinon leukemic cells expressing high level of IL6R.
     为了探索重组IL6-PE40融合蛋白靶向杀伤白血病的效应,我们已成功地将编码人IL6R的全长cDNA转入BNML大鼠急性早幼粒细胞白血病LT12细胞中。
短句来源
     The Study on Recombinant Interleukin 5 as Adjuvant of Zona Pellucida DNA Contraceptive Vaccine in Mucosal Immunization
     重组IL-5作为卵透明带DNA避孕疫苗粘膜免疫佐剂的研究
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  “重组il”译为未确定词的双语例句
     TX IL-6/IL-6-receptor system and recombinan IL 6-PE40 exotoxin fusion protein
     IL-6/IL-6R系统与重组IL-6-PE40融合蛋白
短句来源
     Renaturation and purification of IL-6 D24-PE40KDEL, a novel recombinant IL-6 Pseudomonas exotoxin fusion protein
     新型重组IL-6D24-PE40KDEL外毒素融合蛋白复性及纯化
短句来源
     Optimiziation of the expression and purification for a recombinant IL6-Pseudomonas exotoxin fusion protein IL6D24-PE40KDEL
     新型重组IL6D24-PE40KDEL外毒素融合蛋白的表达优化及下游纯化
短句来源
     Biochemical and Physical Properties for a Recombinant IL6 Pseudomonas Exotoxin Fusion Protein IL6D24 PE40KDEL
     新型重组IL6D24PE40KDEL外毒素融合蛋白的理化分析
短句来源
     Results:IL-1β at 1 nmol/L induced the expression of COX2 and MMP-1 mRNA in human dental pulp cells.
     结果 :人牙髓细胞表达IL 1β受体 ,而且用 1nmol/L人的重组IL 1β明显地刺激牙髓细胞的金属蛋白酶 1、COX2的mRNA表达。
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  相似匹配句对
     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     Construction of recombinant human IL-10 gene
     重组IL-10基因的构建
短句来源
     EXPRESSION OF A NEW TYPE RECOMBINANT HUMAN INTERLEUKIN-2 IN E. COLI
     突变型人重组IL-2的研制
短句来源
     IL?
     说明IL?
短句来源
     Conclusions IL-6 may be involved in the pathologic process of GBS.
     结论IL!
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  recombinant interleukin
The human recombinant interleukin-10 was shown to cleave all forms of plasmid DNA.
      
High-Speed Purification of Recombinant Interleukin-11 by IMAC with Rigid Biporous Beads
      
In the present study it was proved first that human recombinant interleukin-6(HrIL-6) significantly augmented natural killer(NK) cell activity derived from human fetal spleens against K562 target cells in a 4 hours51Cr release assay.
      
TIL could grow for a long-term in medium containing recombinant interleukin-2(rIL-2).
      
Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenm
      
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In the present study we obtained a large guantity of lymphocytes from human gastric carcinoma drained lymph nodes (TdLNL). After culturing with1000u/ml rIL-2 for 5 days, TdLNL became cytotoxic to allogenic humanlung adenocarcinoma cell strain (Ani p-973)in vitro. In a Winn-type assay BALB/c nu/nu mice were inoculated subcutaneousl y with Anip-973 and TdLNL at 10/1 E/T ratio. The results showed that the rIL-2 activiated TdLNL could not only inh(?)t the growth of Anip-973, but also prevent the formation of spontaneous...

In the present study we obtained a large guantity of lymphocytes from human gastric carcinoma drained lymph nodes (TdLNL). After culturing with1000u/ml rIL-2 for 5 days, TdLNL became cytotoxic to allogenic humanlung adenocarcinoma cell strain (Ani p-973)in vitro. In a Winn-type assay BALB/c nu/nu mice were inoculated subcutaneousl y with Anip-973 and TdLNL at 10/1 E/T ratio. The results showed that the rIL-2 activiated TdLNL could not only inh(?)t the growth of Anip-973, but also prevent the formation of spontaneous pulmonary metastases, which suggest that TdLNL might be used as a new LAK source in adoptive immunotherapy for cancer patients.

外科手术中切除的人胃癌引流区淋巴结经细胞分离器分离之后.能获得大量的淋巴细胞(TdLNL)。将TdLNL与基因重组IL—2(rIL—2)共同培养5天后,TdLNL对同种AniP—973肿瘤细胞产生了明显的体外杀伤。同时,rIL—2激活的TdLNL还能抑制An(?)p—933在BALB/c nu/nu裸小鼠皮下的生长和自发性肺转移。

The results of the study proved that the fetal hepatolienal cells activiated by rIL-2 could show a LAK-like activity against human 1ung tumor cel1s in vitro. This LAK-like activity appeared when the concentration of rIL-2 was 200u/ml and increased as the concentration of rIL-2 rose.The percentage of cytotoxicity of fetal hepatolienal cells we(?)e60% and 62% respectively in condition of 1000u/ml and 100: 1 ratio of target to effector cells. If these results were proved in vivo, it would undoubted provide a new...

The results of the study proved that the fetal hepatolienal cells activiated by rIL-2 could show a LAK-like activity against human 1ung tumor cel1s in vitro. This LAK-like activity appeared when the concentration of rIL-2 was 200u/ml and increased as the concentration of rIL-2 rose.The percentage of cytotoxicity of fetal hepatolienal cells we(?)e60% and 62% respectively in condition of 1000u/ml and 100: 1 ratio of target to effector cells. If these results were proved in vivo, it would undoubted provide a new source of LAK cells in clinic.

本研究证明,胎儿肝脾细胞经重组IL-2作用96小时,可产生显著的LAK样活性,在重组IL-2 1000u/ml,E:T为100:1其杀伤率分别达60%和62%。如果这一体外试验结果在体内应用中得到证实,无疑将给临床获取LAK细胞提供一条新的途径。

A sensitive sandwich ELISA for the detection of human IL-2 has been established by using monoclonal and polyclonal antibodies against IL-2. The assay was compatible to the bioassay, with larger detection ranges ( from 1.5u-1 000 u/ml ) . The detergents used in prepartion and purification of the recombinant IL-2 interfered with the assay to different extent, but the sera in the samples did not. Pre-treatment of the plates with lutaralhyde not only decreased the interferece of the detergents, but also increased...

A sensitive sandwich ELISA for the detection of human IL-2 has been established by using monoclonal and polyclonal antibodies against IL-2. The assay was compatible to the bioassay, with larger detection ranges ( from 1.5u-1 000 u/ml ) . The detergents used in prepartion and purification of the recombinant IL-2 interfered with the assay to different extent, but the sera in the samples did not. Pre-treatment of the plates with lutaralhyde not only decreased the interferece of the detergents, but also increased the sensitivity of the ELISA up to 2 folds. The assay could also be ustd in rapid identifying optimal conditions for affinity chromatography of IL-2 and the conformation-epitope relations of the monoclonal antibodies against IL-2.

用抗人白细胞间素-2(IL-2)单克隆抗体(McAb)及多克隆抗体建立了敏感的测定IL-2的夹心ELISA。测定范围为1.5u~1000u/ml,与生物学方法有较好的可比性。十二烷基磺酸钠、盐酸胍、尿素及二巯基乙醇等重组IL-2纯化制备中常用的变性剂对本法有不同程度的干扰,而血清则无。用戌二醛预处理包被板可明显减弱这些试剂的干扰作用,并可将测定的敏感性提高2倍。本法也可用以快速确定亲和层析纯化IL-2的条件及抗IL-2McAb的构相表位相关性。

 
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