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凋亡
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     24 hour after the drug treatment,apoptosis rates of 4μmol/L and 8μmol/L arsenic trioxide groups are 7.58% and 10.18%,they have significant difference with control's(0.83)%(P<0.05).
     4μmol/L As2O3和8μmol/L As2O3组细胞凋亡率分别为7.58%和10.18%,与对照组(凋亡率0.83%)相比差别有显著性意义(P<0.05);
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     RESULTS Apoptosis index (Al) and PCNA labeling indexes (Ll) of the patients with Hp positive peptic ulcer group was higher than those of the patients with Hp negative peptic ulcer group (78.9±5.8 vs 9.0±1.8 and 46.5±7.7 vs 7.9±3.5, p<0.01).
     结果 Hp感染的消化性溃疡患者胃粘膜上皮凋亡指数和增殖指数均明显高于Hp阴性消化性溃疡患者(凋亡指数和增殖指数分别为78.9±5.8vs9.0±1.8和46.5±7.7vs7.9±3.5P<0.01)。
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     Apoptotic rates of different concentrations of VRB treated cells were (3.1± 0.6) %,(7.8±1.2)% and (19.6±4.3)%, respectively, significantly higher than that of control cells(0%, P<0.01).
     流式细胞仪检测VRB处理的肺癌细胞凋亡率分别为 ( 3 .1±0 .6) % ,( 7.8± 1 .2 ) %和( 1 9.6± 4.3 ) % ,较对照组 (凋亡率为 0 )显著增高且呈剂量依赖性 (P <0 .0 1 ) ;
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     The percentage of S phase HL-60 cells decreased from 55.6% to 30% ,the early apoptosis peak appeared (the percentage of apoptosis cells were 25.2%)and the DNA ladders were shown.
     S期细胞百分数由55.6%降至30%,并出现早期凋亡峰(凋亡细胞比例占25.2%);
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     After 8 hours at various concentrations of MTX treatment ranging from 5? nmol/L to 10? μmol/L, the percentage of the cells in the pre-G 1 (apoptotic) was 3.2% at 0.1?
     随 5nmol/L- 10 μmol/LMTX作用 8小时后 ,亚G1(凋亡 )峰的比率从 0 .1μmol/L的 3 2 %增加到 5 0 μmol/L的 18 2 %,S期的比率从 0 0 1μmol/L的 4 1 2 %到 10 μmol/L的 19 1%。
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     Apoptosis of primordial germ cells
     原始生殖细胞的凋亡
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     decreasing chondrocyte apoptosis.
     抑制软骨细胞凋亡
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     (5) apoptosis.
     (5 )凋亡精子。
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     APOPTOSIS IN YEAST
     酵母菌凋亡(英文)
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  (apoptosis
Prodigiosin also could induce apoptosis of pancreatic cancer cells at low concentration and results in the fragmentation pattern of DNA.
      
All these results demonstrate that prodigiosin can obviously inhibit the proliferation of pancreatic cancer cells H8898 by arresting the cell cycle and inducing apoptosis.
      
Detection of the apoptosis of Jurkat cell using an electrorotation chip
      
The apoptosis of cells is one of the fields that attract increasing attention in biology today.
      
Usually, the cells are treated with chemicals when detecting apoptosis.
      
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At various stages of life cycle of cells,e. g. proliferation,differentiation and death,occurrence of DNA strand breaks(DSB)is a common event.For several years,we have been investigating DSB in various tissues at different states of differentiation by in situ nick translation (INT),leading to a finding that there are two tpyes of DSB,one is readily detected by INT,and the other is difficult.INT was performed under the conditions in that,in normal small intestine,the nuclei of terminally differentiated epithelial...

At various stages of life cycle of cells,e. g. proliferation,differentiation and death,occurrence of DNA strand breaks(DSB)is a common event.For several years,we have been investigating DSB in various tissues at different states of differentiation by in situ nick translation (INT),leading to a finding that there are two tpyes of DSB,one is readily detected by INT,and the other is difficult.INT was performed under the conditions in that,in normal small intestine,the nuclei of terminally differentiated epithelial cells at the tip of villi is stained,whereas the nuclei of S phase epithelial cells in the crypt remain unstained and the latter become stainable only with proteinase K treatment.Specifically, frozen sections of the tissues were fixed with ethanol/acetic acid and INT was carried out at 37℃ for 3 hrs with 20μmol/L biotin-11-dUTP and 200IU /ml DNA polymerase 1,as described previously.In this study,we also investigated which type the DSB associated with apoptosis and necrosis belong to,As models of apoptosis and necrosis,rat thymus with an i.p.injection of hydrocortisone(10 mg/100 g BW) and rat livers dama ged with CCl4(100 μl/100 g BW) were used,respectively.It was found that the nuclei of steroidtreated thymocytes in the cortex were stained only when treated with proteinase K, while the hepatocyte nuclei treated with CCl4 were stained without protease digestion, These results indicate that the environment and/or nature of DSB in apoptosis differ from that of necrosis and INT is a convenient molecular histochemical tool to discriminate both types of cell death.

用原位缺口翻译法(INT)比较了细胞凋亡和坏死所伴随的DNA断裂(DSB)类型。INT实验条件为:取大鼠小肠制备冰冻切片,乙醇/醋酸固定,加20μmol/Lbiotin-11-dUTP、0.2IU/LDNA聚合酶Ⅰ,于37℃中孵育3h。正常大鼠小肠绒毛顶端上皮细胞可迅速被染色;而位于肠绒毛基底部的S期上皮细胞则不着色,仅在用蛋白酶K处理后才着色。细胞凋亡标本取自皮下注射氢化可的松(10mg/100g体重)的大鼠胸腺;细胞坏死用CCl4(100μl/100g体重)处理的大鼠肝脏。结果表明,经激素处理的胸腺皮质细胞核只有在蛋白酶K处理后才被染色,而CCl4处理后的肝细胞核不必用蛋白酶K消化就能直接显色。说明细胞凋亡的DSB显色特性不同于细胞坏死。

poptosis.programmed cell death.is a natu-ral form of cell death characterized by activeParticipated of a cell in the process leading toits own decrepit and death. Recently.studiessuggested that apoptosis is a result from a setof discrete cellular events that are regulated bya cascade gene expression.Oncogenes and tu-mor suppressor genes are involved in this regu-lation. Apoptosis is closely related to cancer.Failure and bolckage of apoptosis in tumorcells could therefore be the fundamental impor-tance in contributing...

poptosis.programmed cell death.is a natu-ral form of cell death characterized by activeParticipated of a cell in the process leading toits own decrepit and death. Recently.studiessuggested that apoptosis is a result from a setof discrete cellular events that are regulated bya cascade gene expression.Oncogenes and tu-mor suppressor genes are involved in this regu-lation. Apoptosis is closely related to cancer.Failure and bolckage of apoptosis in tumorcells could therefore be the fundamental impor-tance in contributing not only to the evasion ofphysiological countrols on cell numbers.butalso to the resistance both to natural defensesand to clinical therapy.

细胞凋亡是细胞衰老、死亡过程的主要形式。最近研究发现有多种癌基因与抑癌基因参与细胞凋亡过程。因此目前认为癌基因与抑癌基因不仅控制细胞增殖,分化,而且调节细胞凋亡。细胞凋亡受阻或缺陷可能是肿瘤发生的基础之一。

e apphed more sensitive

采用高敏感多重PAP免疫细胞化学技术,研究抗细胞凋亡基因bcl-2在淋巴结何杰金氏病中的表达与分布。结果表明,21例中有7例(33%)可检出bcl-2蛋白阳性的Reed-Sternberg(R-S)细胞;其中2例(混合细胞型)尚含大量反应性淋巴滤泡,其生发中心也呈阳性反心。相反4例作为阳性对照的炎性淋巴滤泡增生中,bcl-2蛋白主要分布于富含长寿命(long-lived)B细胞的套区(mantlezone),而生发中心则均为阴性。结果提示,bcl-2基因高表达介导的细胞凋亡障碍可能与R-S细胞发生有关。

 
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