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代骨髓
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  primary bone marrow
     Methods:The leukemia cell lines included K562, SHI, Jurkat, NB4, NB4/WT1A, and NB4/WT1D cells, etc. The primary bone marrow cells were collected from 79 patients initially diagnosed as acute leukemia. The expression levels of WT1 gene and WT1(17AA+) isoform were determined by real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR).
     方法:建立实时定量RT-PCR方法,检测K562、SHI、Jurkat、NB4、NB4/WTIA、NB4/WTID等白血病细胞株及79例初诊急性白血病患者原代骨髓细胞中WT1基因和WT1(17AA+)剪接变异体表达水平,并计算WT1(17AA+)/WT1比值。
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     Primary bone marrow stromal cells associated with cytokines induce ES-D_3 cells to differentiate to hematopoietic cells in vitro
     原代骨髓基质细胞联合细胞因子体外诱导ES-D_3细胞造血分化的研究
短句来源
     Objective:To investigate the expression pattern of Wilms tumor gene WT1(17AA+) isoform in different leukemia cell lines and primary bone marrow cells from patients with different subtypes of acute leukemia.
     目的:探讨不同白血病细胞株及不同亚型急性白血病患者原代骨髓细胞中WT1(17AA+)剪接变异体的表达水平。
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     Nucleofection of primary bone marrow stromal cells of rabbit with pEGFP-C2
     pEGFP-C2基因核转染兔原代骨髓基质细胞的实验研究(英文)
短句来源
     ③ The primary bone marrow stromal cells of newborn New Zealand rabbits were isolated and cultured, at the same time treated with permanent-magnetic field of 0.38 and 0.48 T, and the effect of permanent-magnetic field on the osteogeneic ability and lipoblast ability, and compared with those in the without magnetic field group.
     ③分离、培养新生新西兰兔原代骨髓基质细胞,培养的同时分别进行0.38,0.48T恒定磁场处理,观察恒定磁场对骨髓基质细胞成骨分化(其指标为碱性磷酸酶活性)和成脂过程的影响,并与无磁场组进行对比。
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  “代骨髓”译为未确定词的双语例句
     RESULTS 17 β-estradiol promoted the proliferation of primary mouse bone marrow stromal cells and primary mouse osteoblasts at the concentrations of 1×10~ -8 , 1×10~ -7 , 5×10~ -7 , 1×10~ -6 , 5×10~ -6 and 1×10~ -5 mol·L~ -1 (P<0.05).
     结果1×10-8,1×10-7,5×10-7,1×10-6,5×10-6和1×10-5mol·L-1的17β-雌二醇可以促进小鼠原代骨髓基质细胞和成骨细胞增殖(P<0.05);
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     ④Detection of cell surface markers showed that of all the cells 89.6% CD105 positive,68.7% CD29 positive,only 3% CD34 positive and 3% CD45 positive in the 3 passage MSCs.
     ④细胞表面标记物检测结果:第3代骨髓间充质干细胞3%表达CD45,68.7%表达CD29,3%表达CD34,89.6%表达CD105。
短句来源
     Methods: MSCs of the third passage were induced by minerlization condition medium (φ=10%FBS,10 nmol/L dexamethasone,50 mg/L L-vitamin C and 10 mmol/L β-sodium glycerophosphate in DMEM). The control cells were cultured in DMEM with φ=10% FBS.
     方法 :采用矿化液 ( φ =10 %FBS、10nmol/L地塞米松、5 0mg/LL 维生素C和 10mmol/Lβ 甘油磷酸钠的DMEM培养液 )诱导体外培养第 3代骨髓基质细胞 ,以含 φ =10 %FBS的DMEM培养基培养第 3代骨髓基质细胞作对照。
短句来源
     ③Approximately 3×109 L-1 3 to 5 passage BMSCs in 1 mL total fluid volume were injected into a tail vein of rats in BMSCs transplantation group 24 hours after MCAO.
     ③造模24h后,细胞移植组取3~5代骨髓基质细胞,消化离心后抽取1mL骨髓基质细胞悬液(浓度为3×109L-1)于鼠尾静脉输入。
短句来源
     The double time of bone marrow-derived IC was about 38 h and that of venous wall IC about 36 h; The content of hydroxyproline of bone marrow-derived IC medium was (1.518±0.206) mg/L and that of venous IC (1.432± 0.204) mg/L.
     第3代骨髓来源IC倍增时间为38h、上清液羟脯氨酸含量为(1.518±0.206)mg/L,静脉源IC为36h、(1.432±0.204)mg/L两者差异均无统计学意义(P>0.05);
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  相似匹配句对
     So the G-code grou-ping and its implementation is described in detail.
     G
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     On "Generation
     “”论
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     Bone Marrow Necrosis
     骨髓坏死
短句来源
     Methods: rMSCs were isolated from adult rat bone marrow, and expanded in undifferentiated state for 6 passages.
     方法:从成年大鼠骨髓分离、扩增rMSCs至6
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     Cells used in the experiment were harvested from the fourth to sixth passage.
     以第4一6骨髓基质细胞进行有关实验。
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  primary bone marrow
Patients presenting primary bone marrow involvement or bone metastases at diagnosis herald a 3-year disease-free survival below 15%.
      
HAV antigen was detected by APAAP stain in a subpopulation of stromal cells, and sequential estimations of virus titers in the supernatants provided evidence for viral replication in primary bone marrow cultures.
      
Chromosomal measurements differed from data for primary bone marrow cells of this species published by Shaw and Krooth.
      
In high density cultures of primary bone marrow cells, and in the presence of glucocorticoids, bFGF stimulates the formation of a bone-like matrix; however, due to the dense nature of these cultures, the exact mechanism of action is unclear.
      
Both the J774.1 macrophage-like cell line and primary bone marrow derived macrophages were used.
      
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In order to explore the feasibility of gene transfer into hematopoietic stem cells mediated by using a safe, high-efficiency and easy, non-viral transduction protocol, we have transduced two marker genes (Neo R and Lac Z) into hematopoietic cells mediated by lipofectin. After selection with G418, the transduced positive cells were riched. Then we observed the efficiency of gene transfer in primary bone marrow cells of mouse and the stability of gene expression. The results show that the high-efficiency of short-(transient)...

In order to explore the feasibility of gene transfer into hematopoietic stem cells mediated by using a safe, high-efficiency and easy, non-viral transduction protocol, we have transduced two marker genes (Neo R and Lac Z) into hematopoietic cells mediated by lipofectin. After selection with G418, the transduced positive cells were riched. Then we observed the efficiency of gene transfer in primary bone marrow cells of mouse and the stability of gene expression. The results show that the high-efficiency of short-(transient) and long-term (stable) gene transfer can be obtained in the hematopoietic cells of mouse mediated by liposome. Moreover, the transduced positive cells can be riched by G418 selection. The results indicate the feasibility of gene transfer into hematopoietic stem cells mediated by liposome, a non-viral transduction protocol.

为探索一种安全、有效、简易的非病毒基因转移策略用于介导造血干细胞基因转移的可行性,本研究利用商品化的脂质体将两个标志基因(Neo R和Lac Z)共转导造血细胞。通过G418筛选、富集转导阳性细胞,观察了外源基因在小鼠原代骨髓细胞的基因转移率及其表达的稳定性。结果显示:通过脂质体介导,外源基因在小鼠骨髓细胞可获得有效的瞬时和稳定的表达。同时,经G418筛选,转导阳性细胞可得到明显的富集。提示利用脂质体这一非病毒载体个导造血干细胞基因转移的可行性。

A main complication of chemotherapy in cancer patients is hematopoiesis suppression. Microenviroment transplantation using bone marrow stromal cells (BMSCs) has been demonstrated to be a potent method in recovery of hematopoiesis in animal models. Based on hematopoiesis-supportive ability of BMSCs and high potency of IL-3 in hematopoiesis stimulation, BMSCs were studied as a cellular delivery system for IL-3 gene transfection to promote hematopoiesis recovery of mice after high dose chemotherapy. BMSCs were...

A main complication of chemotherapy in cancer patients is hematopoiesis suppression. Microenviroment transplantation using bone marrow stromal cells (BMSCs) has been demonstrated to be a potent method in recovery of hematopoiesis in animal models. Based on hematopoiesis-supportive ability of BMSCs and high potency of IL-3 in hematopoiesis stimulation, BMSCs were studied as a cellular delivery system for IL-3 gene transfection to promote hematopoiesis recovery of mice after high dose chemotherapy. BMSCs were transfected with recombinant adenovirous containing murine IL-3 gene(MOI = 10), the level of mIL-3 secreted by gene-modified BMSCs was 110U/ml/10~6 cells/ 24h in vitro. The mice were injected with high dose cyclophosphamide(200mg/kg) i.p. and after 24 hours the IL-3 gene-modified BMSCs(2 x 10~6/mouse) were transplanted intrasplenically. White blood cell counts in peripheral blood of mice received intrasplenic injection of IL-3-BMSCs were kept at a high level within two weeks after chemotherapy. The pathological sections of spleens and bone marrow showed significant recovery of hematopoiesis, compared with that of mice received chemotherapy only. The data indicated the feasibility of IL-3 gene-modified BMSCs transplantation in the acceleration of hematopoiesis recovery after chemotherapy.

为研究基因治疗在造血功能损伤后恢复中的应用,本课题以携带小鼠IL-3基因的复制缺陷型重组腺病毒载体转染骨髓基质细胞,对大剂量化疗后的小鼠进行脾内移植观察造血功能的恢复情况.结果表明,缺陷型腺病毒载体能有效地转染小鼠原代骨髓基质细胞,转染效率在80%以上(MOI=10);基因修饰的骨髓基质细胞体外分泌IL-3的水平可达110U/ml/10~6细胞/24小时;在大剂量环磷酰胺治疗后脾内移植IL-3基因修饰的基质细胞能有效地升高实验小鼠外周血白细胞总数;病理检测发现IL-3基因修饰的基质细胞治疗组小鼠脾脏和骨髓中细胞增生较其它组明显活跃;经IL-3基因修饰的基质细胞治疗组小鼠脾淋巴细胞对ConA反应明显增强.结果提示IL-3基因修饰的骨髓基质细胞体内移植对大剂量化疗后机体造血与免疫功能的恢复都有较好的促进作用.

Bone forming ability of cultured bone marrow cells under mineralized condition in vitro was observed. The results showed that 7%-8% bone marrow cells of the third passage were alkaline phosphate positive. Obseverd by inverted microscope, cells occupied the whole bottom of cell culture bottle in about 5-6 days and began to grow with the manner of bilayers after 10 days, and on the 14 th and 15 th days, small white nodules could be seen on the bottom of bottle. When cultured up to 30 days, Von Kossa staining...

Bone forming ability of cultured bone marrow cells under mineralized condition in vitro was observed. The results showed that 7%-8% bone marrow cells of the third passage were alkaline phosphate positive. Obseverd by inverted microscope, cells occupied the whole bottom of cell culture bottle in about 5-6 days and began to grow with the manner of bilayers after 10 days, and on the 14 th and 15 th days, small white nodules could be seen on the bottom of bottle. When cultured up to 30 days, Von Kossa staining showed calcium deposition in nodules. Observed by scaning electronic microscope, collagen fibers formed, on which mineral deposited and the trabecular like bone structure formed at the 30 th day. The results suggest that the bone forming ability of cultured bone marrow cells under mineral condition in vitro can be used in tissue engineering.

通过体外矿化条件观察培养兔骨髓细胞的成骨能力,结果显示:①第3代骨髓细胞碱性磷酸酶染色,7%~8%阳性;②倒置显微镜下观察,细胞传代5~6d后,长满培养瓶的底部,约10d开始叠层生长,为局灶状,中央出现较多颗粒样改变,14~15d时,培养瓶底部肉眼可见白色小结节形成,并逐渐增大;③细胞连续培养30d,Von-Kaosa染色显示有局灶状钙盐沉积;④SEM观察,细胞呈叠层生长,形成粗大的胶原纤维束,上有钙盐沉淀,30d时,形成骨小梁样结构。上述结果表明,骨髓细胞体外成骨可以用于组织工程

 
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