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培养的结果
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  results of culture
     Conclusions As a new method for diagnosis of urinary tract infection,UF-100 can detect bacteriuria if cut-off value is bacteria at≥6 000/μl . In addition. this method can predict results of culture rapidly and exactly,which can raise efficiency of diagnosis by laboratory.
     结论 以细菌数≥6 000/μl为UF-100的阳性闲值检测尿液标本,可以作为一种新的诊断尿路感染的方法,快速准确地预测出培养的结果,从而提高实验室诊断的效率。
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     The results of mM16 were different comparing with that of M16 and mCZB (p<0.05), but there was no difference between M16 and mCZB (p>0.05).
     mM16的效果最好,与M16、mCZB相比差异显著(p<0.05),而M16和mCZB培养的结果之间没有显著性差异(p>0.05)。
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     Analysis of 120 Culture Results in Vaginal Secretion
     120例阴道分泌物培养的结果分析
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     The results of micro-organism culture showed that the degradable rate of the biodegradable film added with spore suspension was higher than that of the biodegradable film without spore suspension.
     微生物培养的结果表明,加混合孢子悬液的生物降解膜降解率比未加的生物降解膜高,加土壤悬液的生物降解膜降解率比未加的生物降解膜高。
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     The results indicate that the mixed strain HJ4 has the highest flocculating rate. The maximal flocculating rate is 94.7% when the relative inoculation is 15%,the medium initial pH is 4.5,C/N is 20 ∶1 and the COD in the wastewater is 12 000 mg/L.
     混合培养的结果表明,具有最高絮凝率的混合菌株为HJ4,其最佳培养条件是:相对接种量为15%,培养基初始pH值为4.5,碳氮比为20∶1,废水的COD为12 000 mg/L,在该条件下所产絮凝剂的絮凝率可达94.7%。
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     To explore the possible mechanisms of tanycytes(TAS)promoting neurons survival and outgrowth in vitro, the neurons from cortex were cocultured with TAS and compared with those cocultured with olfactory ensheathing cells (OEC), astrocytes(AST).
     将皮层神经元与传代培养的伸长细胞(tanycytes,TAS)共培养,并与嗅球成鞘胶质细胞(olfactory ensheathing cells,OEC)、星形胶质细胞(astrocytes,AST)共培养的结果相比较,探讨TAS促进共培养神经元生长的机制。
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     The preliminary results showed that the sensitivity of cultured P.b.
     结果表明体外培养P. b.
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     the result were NR.
     结果
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     二、 Adhesive trial results:
     结果
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     The paper extends and develops the results of Liu.
     结果.
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     A satisfactory result is obtained by comparing with cellulose acetate.
     结果
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  results of culture
The diagnosis was based on results of culture and the response of the patient to long-term penicillin-derivate therapy after surgical drainage of the suprarenal abscess formation.
      
Results of culture on the enrichment medium were compared with those obtained on routine agar media and on a yeast differential agar which facilitates detection of mixed yeast species by their colony colours.
      
Such results were compared with the results of culture and indicated that the assay is as sensitive as bacteriological methods, though faster.
      
Results of culture of thirty soil samples obtained from the Judean Desert on the western side of the Dead Sea are reported.
      
The results of culture-tests performed on various textiles, the colony-formation of the more important dermatophytes on various textiles are discussed.
      
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Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different...

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different culture media such as PDA, acidified PDA, cotton meal agar and rice agar. All these plates failed to show verticillium colonies. Among the fungi obtained in these plates, Fusarium, Alternaria and Colletotrichum were the most frequent. Since both Verticillium albo-atrum and other fungi were harbored within the same seed, the difference of growth rates might be considered as an important factor involved in the difficulty of the isolations: Cultures of V. albo-atrum and Fusarium sp. were transferred simutaneously to PDA plates to test their growth rates. The diameter of their colonies were measured after incubation for 12 days under 22±1℃. Those of V. albo-atrum measured 2.8 cm., while the average of the latter was 9,3 cm. Interference of the fast-growers might render a slow-grower such as V. albo-atrum to be masked in the observations and to be depressed in the isolations. A method was developed to avoid the interference. The fundamental procedures of the method are outlined as follows. 1. Plating and observation. (1) Cotton seeds being delinted with commercial surphuric acid in order to avoid the interference from lint-born contaminants. (2) Delinted seeds being washed in a flask covered with wire gauze for 24 hours under running tap water in order to get rid of bacteria. Absorption of sufficient water during washing activates the hibernating mycelium. (3) Seeds being plated on 1.7% water agar instead of nutrient agar in order to diminish the growth of the fast-growers such as Fusaria and Alternaria. The plates being incubated under 22±1℃. for 15 days. (4) The plates being uncovered and observed directly under low-power microscope in order to observe the fungi undisturbed. 2. Isolation and preservation of the Verticillium cultures. (1) Verticilliate conidiophores being located under low-power microscope. (2) The tip of a flamed needle moistened with sterile agar being introduced within the microscopic field in order to fish the spores under focus. (3) Spore suspension being made by washing the needle in a 5 cc sterile water blank. (4) Dilution plates being made by placing 1 cc of the spore suspension in a petri dish and diluting with 10 cc of cotton stem agar. (5) The plates being observed under low-power microscope after incubation for 5—7 days at 22±1℃. (6) Verticillium colonies being locatad and a bit of the margin of a colony being transferred to PDA slant. (7) The slant cultures being flooded with sterile liquid paraffin after incubation for 7—15 days at 22±1℃. The cultures preserved with liquid paraffin were able to keep alived for 2 years. Seeds collected from the infected plants in 1955, 1956 and 1957 were studied. The percentages of seeds carrying Verticillium observed in these years were 39.8, 5.92 and 9.5% respectively. One of the representative single spore colonies obtained was identified as V. albo—atrum. Inoculation and reisolation tests showed positive results. Inspection of cotton seed samples collected from eight co-op farms in Shensi Province in 1958 showing that the percentages of seeds infected by verticillium ranged 3 to 23% with an average of 12.3%.

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号...

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。

The effects of the composition and pH of different culture media on the amino acid decarboxylase of five strains of E. coli were studied, and a solid medium consisting of beef broth, peptone(1%) and agar(2%) at pH 7.5 was selected to cultivate the bacteria for preparing L-glutamic acid decarboxylase. An acetone powder was prepared from E. coli grown in the solid medium at 37℃ for 18-20 hours. It decarboxylated L-glutamic acid with a high specificity.

根据五株大腸杆菌在不同成分与pH的培养基內培养的結果,选择了pH7.5的牛肉湯加蛋白腖与琼脂的固体培养作为細菌培养条件。从18—20小时在37度培养的大腸杆菌制成了高产量的专一性谷氨酸脫羧酶丙酮粉,其最适pH为5.1,米氏常数为4.3×10~(-3)M。拟定了一种血清及动物組織中轉氨基酶的測定法。此方法是利用大腸杆菌谷氨酸脫羧酶丙酮粉测定轉氨基作用所生成的谷氨酸量。应用此方法測得我国正常成人血清的谷門轉氨基酶活性为0.70±0.22单位与谷丙轉氨基酶活性为0.58±0.25单位(每单位等于在37度轉氨基作用生成1微克分子L-谷氨酸/1毫升血清/1小时)。应用此方法也試測了大白鼠六种組織中五种氨基酸对α-酮戊二酸的轉氨基酶活性。

o-Bis (β-chloroethyl)-aminomethyl-phenylalanine dihydrochloride (I, AT-581) was re- ported to possess significant antitumour activities. Furthermore, in view of the fact that compounds carrying a nitro group in the benzene ring show invariably higher antitumour activity as compared with the corresponding unsubstituted ones and that the electro- negative groups such as nitro can deactivate the chlorine atoms of the mustard grouping, thus decrease its toxicity, the authors synthesized 2-bis-(β-chloroethyl)-aminomethyl-5-...

o-Bis (β-chloroethyl)-aminomethyl-phenylalanine dihydrochloride (I, AT-581) was re- ported to possess significant antitumour activities. Furthermore, in view of the fact that compounds carrying a nitro group in the benzene ring show invariably higher antitumour activity as compared with the corresponding unsubstituted ones and that the electro- negative groups such as nitro can deactivate the chlorine atoms of the mustard grouping, thus decrease its toxicity, the authors synthesized 2-bis-(β-chloroethyl)-aminomethyl-5- nitro-phenylalanine(IV) in which a nitro group was introduced into the benzene ring of AT-581. Besides, 2-bis(β-chloroethyl)-aminomethyl-4-nitrotoluene(IV) was also synthe- sized. 2-Methyl-5-nitrobenzyl chloride (VII) was condensed with diethyl formamido-malo- nate to give diethyl 2-methyl-5-nitrobenzyl formamido-malonate (VIII), and the latter was readily converted to diethyl 2-bromomethyl-5-nitrobenzyl formamido-malonate(X) by N-bromosuccinimide in the presence of a small amount of dibenzoyl peroxide in anhydrous carbon tetrachloride. Treatment of X with diethanolamine afforded diethyl 2-bis(β- hydroxyethyl)-aminomethyl-5-nitrobenzyl formamido-malonate(XI), which was then chlorinated with thionyl chloride in dichloromethane to give the chloride (XII),and the desired product (IV) was obtained by the hydrolysis of XII with concentrated hydro- chloric acid. When compound VII was first treated with diethanolamine and then with thionyl chloride, compound VI was obtained.

在邻双(β-氯乙基)氨甲基苯丙氨酸(I,AT-581)苯环的5-位上引进一个硝基,合成了化合物IV.作者希望能通过硝基的拉电子作用降低氮芥基上氯原子的活泼性,从而减低AT-581的毒性并增加其抗癌活性.在同样的设想下,作者又在邻甲基苄基氮芥(V)苯环的4-位上引入一个硝基,合成了化合物VI.组织培养结果表明化合物IV和VI对HeLa细胞具有抑制作用.

 
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