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外周血抗hcgβ抗体
相关语句
  anti hcg β antibody
     The anti hCGβ antibody titers were determined by indirect ELISA in 6 weeks of last immunization.
     末次免疫后 6周采血 ,用间接ELISA分析实验动物外周血抗hCGβ抗体效价。
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  “外周血抗hcgβ抗体”译为未确定词的双语例句
     The anti-hCGβ antibody titers were determined by indirect ELISA in 6 weeks of last immunization.
     于末次免疫后 6周采血 ,用间接ELISA分析实验动物外周血抗hCGβ抗体效价。
短句来源
     The titers of serum and subclasses of anti-hCGβ IgG antibody were determined by indirect enzyme-linked immunosorbent assay(ELISA).
     间接ELISA法分析免疫小鼠外周血抗hCGβ抗体动态变化以及IgG亚型。
短句来源
     Stimulated in vitro by 5 000 IU hCGβ antigen, the splenetic cells of the C3 and D3 immunization group produced significantly lower IL-2, INF-γ and TNF-α than those of the B3 immunization group (P<0.01 or P<0.05).
     于第 2次免疫后 3周 ,处死小鼠。 用ELISA法测定外周血抗hCGβ抗体效价和经hCG抗原体外刺激脾细胞培养上清中Th1型 (IL 2、INF γ、TNF α) /Th2型 (IL 4、IL 10 )细胞因子分泌水平。
短句来源
  相似匹配句对
     The titers of serum and subclasses of anti-hCGβ IgG antibody were determined by indirect enzyme-linked immunosorbent assay(ELISA).
     间接ELISA法分析免疫小鼠外周hCGβ体动态变化以及IgG亚型。
短句来源
     Age-associated Changes of Antibodies against β-amyloid Protein in Plasma of Healthy Chinese Population
     国人外周β-淀粉样蛋白体水平的依龄性变化
短句来源
     PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST hCG-β
     hCG-β单克隆体的产生及初步鉴定
短句来源
     The anti hCGβ antibody titers were determined by indirect ELISA in 6 weeks of last immunization.
     末次免疫后 6周采 ,用间接ELISA分析实验动物外周hCGβ体效价。
短句来源
     The anti-hCGβ antibody titers were determined by indirect ELISA in 6 weeks of last immunization.
     于末次免疫后 6周采 ,用间接ELISA分析实验动物外周hCGβ体效价。
短句来源
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Objective To testify the effect of C3d molecular adjuvant on the immunogenicity of human chorionic gonadotropin β (hCGβ) DNA vaccination as well as the mode of immune response. Methods BALB/c mice aged 6 weeks were immunized intramuscularly two times at an interval of 3 weeks with by the plasmid pcDNA3 (A1~3 groups), pcDNA3-hCGβ (B1~3 groups), pcDNA3-hCGβ-C3d3 (C1~3 groups), or pCMV4-hCGβ-C3d3 (D1~3 groups), at dosage of 5 pmol, 10 pmol, and 20pmol, respectively. Three weeks after the second vaccination...

Objective To testify the effect of C3d molecular adjuvant on the immunogenicity of human chorionic gonadotropin β (hCGβ) DNA vaccination as well as the mode of immune response. Methods BALB/c mice aged 6 weeks were immunized intramuscularly two times at an interval of 3 weeks with by the plasmid pcDNA3 (A1~3 groups), pcDNA3-hCGβ (B1~3 groups), pcDNA3-hCGβ-C3d3 (C1~3 groups), or pCMV4-hCGβ-C3d3 (D1~3 groups), at dosage of 5 pmol, 10 pmol, and 20pmol, respectively. Three weeks after the second vaccination the animals were killed, specimens of their peripheral blood were extracted to determine the anti-hCGβ antibody titer by indirect ELISA. Their spleen cells were harvested and stimulated in vitro by hCG antigen for 24 hours. The Th1/Th2 cytokines in the culture supernatant were determined by ELISA. Results At the dosage of 20 pmol, C3d molecular adjuvant significantly enhanced the anti-hCGβ antibody titer. The utmost anti-hCGβ antibody titer of C3 group was 1∶450, 9 times higher than that of B3 group, and the utmost anti-hCGβ antibody titer of D3 group was 1∶12 150, 243 times higher than that of B3 group. Stimulated in vitro by 5 000 IU hCGβ antigen, the splenetic cells of the C3 and D3 immunization group produced significantly lower IL-2, INF-γ and TNF-α than those of the B3 immunization group (P<0.01 or P<0.05). The IL-4 level of the C3 group was higher than that of the B3 group while the IL-10 level of the D3 group was significantly higher than that of the B3 group (P<0.01). Conclusions The C3d molecular adjuvant increases significantly the hCGβ immunogenicity of hCGβ DNA vaccination; meanwhile decreases the secretion of Th1 cytokines(IL-2, INF-γ, and TNF-α), and increases the expression of Th2 cytokines(IL-4 and IL-10)in response to hCG antigen. So C3d changes the anti-hCG immune response from Th1 type to Th2 type.

目的 研究分子佐剂C3d能否增强人绒毛膜促性腺激素 (hCG) βDNA疫苗的免疫原性 ,并转变Th1/Th2型免疫应答模式。方法 抽提纯化pcDNA3、pcDNA3 hCGβ、pcDNA3 hCGβ C3d3、pCMV4 hCGβ C3d34种质粒。将 12只 6周龄BALB/c雌性小鼠分为 12组 ,A1~ 3组各 6只以pcDNA3免疫 ,作为空白对照组 ;B1~ 3组各 6只以pcDNA3hCGβ免疫 ,作为阴性对照组 ;C1~ 3组各 6只以pcDNA3 hCGβ C3d3免疫 ,作为实验 1组 ;D1~ 3组各 5只以pCMV4 hCGβ C3d3免疫 ,作为实验 2组。免疫前 1周 ,于小鼠左后腿肌肉注射 0 2 5 %普鲁卡因 0 1ml。 1周后 ,在相同部位分别肌注前述 4种质粒 ,剂量分别为 :5pmol(A1~D1组 )、10pmol(A2~D2组 )、2 0pmol(A3~D3组 )。 3周后 ,再次给予相同剂量质粒加强免疫。于第 2次免疫后 3周 ,处死小鼠。用ELISA法测定外周血抗hCGβ抗体效价和经hCG抗原体外刺激脾细胞培养上清中Th1型 (IL 2、I...

目的 研究分子佐剂C3d能否增强人绒毛膜促性腺激素 (hCG) βDNA疫苗的免疫原性 ,并转变Th1/Th2型免疫应答模式。方法 抽提纯化pcDNA3、pcDNA3 hCGβ、pcDNA3 hCGβ C3d3、pCMV4 hCGβ C3d34种质粒。将 12只 6周龄BALB/c雌性小鼠分为 12组 ,A1~ 3组各 6只以pcDNA3免疫 ,作为空白对照组 ;B1~ 3组各 6只以pcDNA3hCGβ免疫 ,作为阴性对照组 ;C1~ 3组各 6只以pcDNA3 hCGβ C3d3免疫 ,作为实验 1组 ;D1~ 3组各 5只以pCMV4 hCGβ C3d3免疫 ,作为实验 2组。免疫前 1周 ,于小鼠左后腿肌肉注射 0 2 5 %普鲁卡因 0 1ml。 1周后 ,在相同部位分别肌注前述 4种质粒 ,剂量分别为 :5pmol(A1~D1组 )、10pmol(A2~D2组 )、2 0pmol(A3~D3组 )。 3周后 ,再次给予相同剂量质粒加强免疫。于第 2次免疫后 3周 ,处死小鼠。用ELISA法测定外周血抗hCGβ抗体效价和经hCG抗原体外刺激脾细胞培养上清中Th1型 (IL 2、INF γ、TNF α) /Th2型 (IL 4、IL 10 )细胞因子分泌水平。结果 C3d分子佐剂能明显提高抗hCGβ抗体滴度。当免疫剂量为 2 0pmol时 ,与B3组比较 ,C3组抗hCG抗体最大效价达到 1∶4 5 0 ,提高了 9倍。D3组抗hCG抗体最大效价达到 1∶12 15 0 ,提高了 2 4 3倍。D3组与C3组相比较 ,抗hCG抗体水平提高了 2 7倍。受

Objective:In the past study, a plasmid of pcDNA3 hCGβ C3d3 had been constructed. It was suggested that the fusing protein be expressed by the pcDNA3 hCGβ C3d3 plasmid both in the transient expression system in COS 7 cells, and the stable expression system in CHO cells. In the present research, it would be testified that the C3d molecular adjuvant could improve the immunogenicity of the hCGβ DNA immunization or not. Methods:BALB/C mice of 6 weeks old were immunized intramuscularly two times at interval of...

Objective:In the past study, a plasmid of pcDNA3 hCGβ C3d3 had been constructed. It was suggested that the fusing protein be expressed by the pcDNA3 hCGβ C3d3 plasmid both in the transient expression system in COS 7 cells, and the stable expression system in CHO cells. In the present research, it would be testified that the C3d molecular adjuvant could improve the immunogenicity of the hCGβ DNA immunization or not. Methods:BALB/C mice of 6 weeks old were immunized intramuscularly two times at interval of 3 weeks by the plasmid pcDNA3, pcDNA3 hCGβ, pcDNA3 hCGβ C3d3 at dosage of 5, 10, 20 pmol, respectively. The anti hCGβ antibody titers were determined by indirect ELISA in 6 weeks of last immunization. Results:The result showed that the C3d molecular adjuvant could increase significantly the titer of anti hCGβ antibody in a dose dependent manner after DNA immunization.Conclusion:The C3d molecular adjuvant does improve the humoral immunity of the hCGβ DNA immunization, which would be helpful for technical progress and clinical trial of the contraceptive vaccine. [

目的 :该实验室前期工作已成功构建真核表达质粒pcDNA3 hCGβ、pcDNA3 hCGβ C3d3且证明其能在真核表达系统中有效表达。通过动物DNA免疫 ,以期证实分子佐剂C3d增强免疫避孕疫苗免疫原性。方法 :抽提与纯化pcDNA3、pcD NA3 hCGβ、pcDNA3 hCGβ C3d3质粒 ,进行动物DNA免疫。免疫剂量分别为 5、10、2 0pmol,共免疫 2次 ,每次间隔 3周。末次免疫后 6周采血 ,用间接ELISA分析实验动物外周血抗hCGβ抗体效价。 结果 :C3d分子佐剂能明显提高抗hCGβ抗体滴度 ;且其作用呈剂量依赖性。结论 :分子佐剂确实能增强hCGβ免疫原性 ;此结果将有助于免疫避孕疫苗的技术进步及实际应用。

Objective:To compare the expression and immunizing efficiency of the hCGβ-C3d3 fusion protein between pCMV4 and pcDNA3.Methods:The plasmid pCMV4-hCGβ-C3d3 was constructed from pcDNA3-hCGβ-C3d3.COS-7 cells were transfected with pCMV4-hCGβ-C3d3 and pcDNA3-hCGβ-C3d3,and the expression efficiency in vitro was compared between them.BALB/C mice of 6 weeks old were immunized intramuscularly by pcDNA3,pcDNA3-hCGβ-C3d3,pCMV4-hCGβ-C3d3 ,respectively.The anti-hCGβ antibody titers were determined by indirect ELISA in 6...

Objective:To compare the expression and immunizing efficiency of the hCGβ-C3d3 fusion protein between pCMV4 and pcDNA3.Methods:The plasmid pCMV4-hCGβ-C3d3 was constructed from pcDNA3-hCGβ-C3d3.COS-7 cells were transfected with pCMV4-hCGβ-C3d3 and pcDNA3-hCGβ-C3d3,and the expression efficiency in vitro was compared between them.BALB/C mice of 6 weeks old were immunized intramuscularly by pcDNA3,pcDNA3-hCGβ-C3d3,pCMV4-hCGβ-C3d3 ,respectively.The anti-hCGβ antibody titers were determined by indirect ELISA in 6 weeks of last immunization.Results:There was a significant difference of expression efficiency in vitro in COS-7 cells between the pcDNA3-hCGβ-C3d3 and pCMV4-hCGβ-C3d3( P <0.01).The mice produced significantly higher anti-hCGβ antibody level after DNA immunization with pcDNA3-hCGβ-C3d3 and pCMV4-hCGβ-C3d3, than that with pcDNA3-hCGβ-C3d3( P <0.05).Conclusion:The expression efficiency of pCMV4 was much higher than that of pcDNA3,and pCMV4 can induce stronger immune response than pcDNA3 because of its high expression efficiency.

目的 :比较以pCMV4和pcDNA3为载体的hCGβ C3d3DNA疫苗的表达效率和免疫效力。 方法 :分别构建pcD NA3 hCGβ C3d3、pCMV4 hCGβ C3d3真核细胞表达质粒 ,并转染真核细胞瞬时表达系统COS 7细胞 ,以分析体外表达效率。抽提与纯化pcDNA3、pcDNA3 hCGβ C3d3、pCMV4 hCGβ C3d3质粒。对 6周龄BLAB C小鼠行DNA免疫。于末次免疫后 6周采血 ,用间接ELISA分析实验动物外周血抗hCGβ抗体效价。 结果 :对COS 7体外表达效率分析 ,由pCMV4介导的hCGβ C3d3表达效率明显高于pcDNA3。动物DNA免疫结果 ,pCMV4 hCGβ C3d3刺激动物机体产生的免疫应答强度及免疫效果显著高于pcDNA3 hCGβ C3d3。 结论 :pCMV4真核表达质粒hCGβ C3d3体外表达效率及动物DNA免疫效力均显著高于pcDNA3真核表达质粒。

 
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