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检测乙型
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  detection of hepatitis b
     Detection of Hepatitis B Serum HBV-DNA by Quantitative PCR and Its Clinical Application
     定量聚合酶链反应检测乙型肝炎患者血清HBV-DNA及其临床应用
短句来源
     Detection of Hepatitis B Virus Using Molecular Beacon and Asymmetric PCR
     分子信标用于不对称PCR检测乙型肝炎病毒
短句来源
     Objects To establish a mismatched polymerase chain reaction restricted fragment length polymorphism(mPCR-RFLP) method for the detection of hepatitis B virus (HBV) mutations in precore A1896,basic core promoter(BCP)T1762/A1764 and compare the method with direct sequencing to evaluate its application value.
     目的建立错配聚合酶链反应-限制性片段长度多态性(mPCR-RFLP)检测乙型肝炎病毒(HBV)前C区A1896、基本核心启动子(BCP)T1762/A1764双变异的方法,与直接测序法比较,评估其应用价值。
短句来源
     Applying Competitive Polymerase Chain Reaction to the Detection of Hepatitis B Virus DNA
     应用竞争聚合酶链反应检测乙型肝炎病毒DNA
短句来源
     Establishment and preliminary application of a gene chip for detection of hepatitis B virus "a" determinant hotpoint mutation
     检测乙型肝炎病毒“a”决定簇热点突变基因芯片的制备和初步应用
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  “检测乙型”译为未确定词的双语例句
     HBVM, CD + 3, CD + 4, CD + 4/CD + 8, IFN-α and IL-6 were measured before and after being treated.
     在治疗前后分别检测乙型肝炎病毒标志 (HBVM) ,CD+ 3,CD+ 4 ,CD+ 4 /CD+ 8,α-干扰素 (IFN- α)及白细胞介素 - 6 (IL- 6 )水平 .
短句来源
     Comparison of the Detection of Serum HBV DNA between by Fluorescent Quantitative PCR and by Common Qualitative PCR
     荧光定量PCR与普通定性PCR检测乙型肝炎血清HBV DNA结果的比较
短句来源
     Detection of the HBV DNA with Sybr green 1 real-time polymerase chain reaction
     Sybr green1实时定量PCR检测乙型肝炎病毒DNA的探索
短句来源
     Detection of Serum HBV DNA in Patients with Hepatitis B by Quantitative PCR and It's Significance
     定量PCR检测乙型肝炎患者血清HBV DNA及其临床意义
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     HBV DNA detection with oligodeoxynucleotide modified Fe_3O_4(core)/Au(shell) nanoparticle probes
     Fe_3O_4(核)/Au(壳)纳米颗粒探针的制备及其在检测乙型肝炎病毒DNA中的应用
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  相似匹配句对
     Selective detection of hepatitis B virus mRNA by random amplification cDNA ends
     肝炎病毒mRNA的选择性检测
短句来源
     Analysis for Detection Results of YMDD Mutants in HBV and Its Clinical Values
     肝炎病毒基因突变检测分析
短句来源
     Hepatitis B Virus
     肝炎病毒
短句来源
     Radioimmunoassay of Hepatitis B in the Serum Samples of Bactrian Camel (Camelus bactrianus)
     双峰驼类人肝炎的检测
短句来源
     Detection of YMDD mutations in HBV polymerase gene and study of clinical practice
     肝炎病毒P基因变异的检测及其意义
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  detection of hepatitis b
Detection of hepatitis B surface antigen in fixed tissues of patients with cirrhosis and hepatoma
      
Here we describe how detection of hepatitis B virus surface antigen, a marker of these cells, has led to identification of pancreatic and muscle metastases.
      
Sensitivity of seven commercial assays in the detection of hepatitis B virus Type 2-like infection
      
Detection of hepatitis B virus DNA in chronic carriers by the polymerase chain reaction
      
Detection of hepatitis B virus DNA and determination of surface antigen expression in peripheral blood mononuclear cells from pa
      
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A solid-phase radioimmunoassay (RIA) method was developed for detection of HBsAg. Its reprodu-cibility. sensitivity and specificity were evaluated, and these results were compared with the Abbott kit (AUSRIA).Horse anti-HBs was used both for coating solid phase and iodination. Normal horse serum was applied in the labelled antiserum to prevent the non-specific reaction between different species of animal sera.The cutoff was 2.21× negative control value. The factor 2.21 was derived from the mean S/N value and...

A solid-phase radioimmunoassay (RIA) method was developed for detection of HBsAg. Its reprodu-cibility. sensitivity and specificity were evaluated, and these results were compared with the Abbott kit (AUSRIA).Horse anti-HBs was used both for coating solid phase and iodination. Normal horse serum was applied in the labelled antiserum to prevent the non-specific reaction between different species of animal sera.The cutoff was 2.21× negative control value. The factor 2.21 was derived from the mean S/N value and its 3 SD of 553 normal sera, while the factor of AUSRIA was 2.1. The lowest detectable amount of HBsAg by our RIA method was 4. 1ng/ml, while that by AUSRIA 2. 1ng/ml, which was about 2 times more sensitive than our system.The prevalence of HBsAg in normal population detected by this RIA ranged from 12.9 to 17.7%. that in the patients with acute viral hepatitis was 60.6%. and in chronic viral hepatitis patients 89.9%.As hepatitis B virus infection is quite common in our country, it is important and urgent to use RIA widely to detect HBsAg carrier in the clinical diagnosis, selection of donors and epidemiological investigation.

本文对固相放射免疫法检测乙型病毒性肝炎表面抗原的可重复性、灵敏性、特异性等作了初步探讨,并与Abbott药盒进行了对照。 应用马抗-HBs作包被固相抗体和标记抗体时,在标记抗体的稀释液中应加入正常马血清,可避免不同种动物之间血清的非特异性反应。 应用对流免疫电泳(CIEP)法可检出的HBsAg含量为1.29μg/ml,我室的SPRIA-HBsAg检测系统可检出的HBsAg含量为4.1ng/ml,而Abbott药盒为2.1ng/ml。我室检测系统的灵敏性为Abbott药盒的1/2。 应用上述检测系统对广州地区正常人群进行了测试,得出常数为2.21,Abbott药盒则为2.1。 HBsAg检出率在正常人群为12.9~17.7%,急性肝炎为60.6%,慢性肝炎为89.9%,说明我国人群乙型肝炎病毒感染极为常见。因此,在临床诊断、筛选献血员及流行病学等方面推广使用这一检测方法尤为重要和迫切。

HBV markers were tested for in 1812 serum samples from populations differing in thier sources of drinking water in Qidong county. The purpose of this survey was to analyze the local distribution of HBV and its relationship to the incidence of primary liver cancer, and also possible associatios with drinking water.826 samples were taken from residents drinking ditch or river water, the incidence of PLC was 86.5/106, 986 samples were from residents consuming well water, the incidence of PLO was 18.9/108, (statistically...

HBV markers were tested for in 1812 serum samples from populations differing in thier sources of drinking water in Qidong county. The purpose of this survey was to analyze the local distribution of HBV and its relationship to the incidence of primary liver cancer, and also possible associatios with drinking water.826 samples were taken from residents drinking ditch or river water, the incidence of PLC was 86.5/106, 986 samples were from residents consuming well water, the incidence of PLO was 18.9/108, (statistically significant). 5 markers were used for testing. The total No. of positive tests were 1077 and 1272 in the 2 groups respectively. The No. of positive markers per person averaged 1.3 and 1.29. The average No. of markers carried by each positive individual averaged 1.82 and 1.87 (not statistically significant).

启东县居民按死亡率分级,随机抽样检测血清1812份,每份血清检测乙型肝炎病毒感染标记(HBVM)。目的是进一步了解不同饮水类型居民原发性肝癌(PLC)发病率与HBVM间的关系和HBVM在居民中的分布情况。调查结果表明,随机抽样调查的826人属于饮用沟、河水地区,该地区的肝癌发病率为86.5/10万;986人属于饮用浅井水和深井水地区,该地区的肝癌发病率为18.9/10万。两个地区肝癌发病率迥然不同,差别有统计学显著性意义。两组对象的HBVM总阳性数分别为1077和1272;两组标记的平均数分别为1.30和1.29;乙肝感染者平均携带标记分别为1.82和1.87。两组以上各指标均无统计学显著差异。

A comparative study has been made on the sensitivity and etecive rate of the three detective techniques about HBsAa and the dSPRIA which has been gradually developed in our country at present. The result from 120 sera samples of hepatitis section shows that the tsensitivity of SPRIA is the highest among four techniques used, the positive rate of HBsAg is 40. 83%; the positive rate with the technique of ELISA is about 33.33%; while that with ciep is 17.50% only.The cross-sectional analysis shows that it is necessary...

A comparative study has been made on the sensitivity and etecive rate of the three detective techniques about HBsAa and the dSPRIA which has been gradually developed in our country at present. The result from 120 sera samples of hepatitis section shows that the tsensitivity of SPRIA is the highest among four techniques used, the positive rate of HBsAg is 40. 83%; the positive rate with the technique of ELISA is about 33.33%; while that with ciep is 17.50% only.The cross-sectional analysis shows that it is necessary to improve the technique of HBsAg detection. In some places and hospitals, if SPRIA can not be carried out at present due to lack of facilities, the HBsAg detective rate can also be raised from 61.22% and 81.63% up to 93.8% so long as RPHA and ELISA are used simultaneously.

对原来用于检测乙型病毒性肝炎(简称乙肝)表面抗原的三种方法和目前正在我国逐步采用的固相放射免疫分析法的敏感性和检出率作了比较研究。检测120份肝病门诊病人血清乙肝表面抗原(HBsAg)的结果表明,在四种方法中,以固相放射免疫分析法的敏感性最高,HBsAg阳性率为40.83%;其次为酶联免疫吸附试验,阳性率为33.33%;以对流免疫电泳法的阳性率最低,仅17.50%,尚不及固相放射免疫法的一半。交叉分组分析的结果说明改进检测技术的必要性;在目前尚没有条件使用固相放射免疫分析技术的地区和医院,如果同时应用反向间接血凝试验和酶联免疫吸附试验两种方法检测,可以将HBsAg检出率从原来的61.22%和81.63提高到93.8%左右。

 
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