Objects To establish a mismatched polymerase chain reaction restricted fragment length polymorphism(mPCR-RFLP) method for the detection of hepatitis B virus (HBV) mutations in precore A1896,basic core promoter(BCP)T1762/A1764 and compare the method with direct sequencing to evaluate its application value.
Detection of hepatitis B surface antigen in fixed tissues of patients with cirrhosis and hepatoma
Here we describe how detection of hepatitis B virus surface antigen, a marker of these cells, has led to identification of pancreatic and muscle metastases.
Sensitivity of seven commercial assays in the detection of hepatitis B virus Type 2-like infection
Detection of hepatitis B virus DNA in chronic carriers by the polymerase chain reaction
Detection of hepatitis B virus DNA and determination of surface antigen expression in peripheral blood mononuclear cells from pa
A solid-phase radioimmunoassay (RIA) method was developed for detection of HBsAg. Its reprodu-cibility. sensitivity and specificity were evaluated, and these results were compared with the Abbott kit (AUSRIA).Horse anti-HBs was used both for coating solid phase and iodination. Normal horse serum was applied in the labelled antiserum to prevent the non-specific reaction between different species of animal sera.The cutoff was 2.21× negative control value. The factor 2.21 was derived from the mean S/N value and...
A solid-phase radioimmunoassay (RIA) method was developed for detection of HBsAg. Its reprodu-cibility. sensitivity and specificity were evaluated, and these results were compared with the Abbott kit (AUSRIA).Horse anti-HBs was used both for coating solid phase and iodination. Normal horse serum was applied in the labelled antiserum to prevent the non-specific reaction between different species of animal sera.The cutoff was 2.21× negative control value. The factor 2.21 was derived from the mean S/N value and its 3 SD of 553 normal sera, while the factor of AUSRIA was 2.1. The lowest detectable amount of HBsAg by our RIA method was 4. 1ng/ml, while that by AUSRIA 2. 1ng/ml, which was about 2 times more sensitive than our system.The prevalence of HBsAg in normal population detected by this RIA ranged from 12.9 to 17.7%. that in the patients with acute viral hepatitis was 60.6%. and in chronic viral hepatitis patients 89.9%.As hepatitis B virus infection is quite common in our country, it is important and urgent to use RIA widely to detect HBsAg carrier in the clinical diagnosis, selection of donors and epidemiological investigation.
HBV markers were tested for in 1812 serum samples from populations differing in thier sources of drinking water in Qidong county. The purpose of this survey was to analyze the local distribution of HBV and its relationship to the incidence of primary liver cancer, and also possible associatios with drinking water.826 samples were taken from residents drinking ditch or river water, the incidence of PLC was 86.5/106, 986 samples were from residents consuming well water, the incidence of PLO was 18.9/108, (statistically...
HBV markers were tested for in 1812 serum samples from populations differing in thier sources of drinking water in Qidong county. The purpose of this survey was to analyze the local distribution of HBV and its relationship to the incidence of primary liver cancer, and also possible associatios with drinking water.826 samples were taken from residents drinking ditch or river water, the incidence of PLC was 86.5/106, 986 samples were from residents consuming well water, the incidence of PLO was 18.9/108, (statistically significant). 5 markers were used for testing. The total No. of positive tests were 1077 and 1272 in the 2 groups respectively. The No. of positive markers per person averaged 1.3 and 1.29. The average No. of markers carried by each positive individual averaged 1.82 and 1.87 (not statistically significant).
A comparative study has been made on the sensitivity and etecive rate of the three detective techniques about HBsAa and the dSPRIA which has been gradually developed in our country at present. The result from 120 sera samples of hepatitis section shows that the tsensitivity of SPRIA is the highest among four techniques used, the positive rate of HBsAg is 40. 83％; the positive rate with the technique of ELISA is about 33.33％; while that with ciep is 17.50％ only.The cross-sectional analysis shows that it is necessary...
A comparative study has been made on the sensitivity and etecive rate of the three detective techniques about HBsAa and the dSPRIA which has been gradually developed in our country at present. The result from 120 sera samples of hepatitis section shows that the tsensitivity of SPRIA is the highest among four techniques used, the positive rate of HBsAg is 40. 83％; the positive rate with the technique of ELISA is about 33.33％; while that with ciep is 17.50％ only.The cross-sectional analysis shows that it is necessary to improve the technique of HBsAg detection. In some places and hospitals, if SPRIA can not be carried out at present due to lack of facilities, the HBsAg detective rate can also be raised from 61.22％ and 81.63％ up to 93.8％ so long as RPHA and ELISA are used simultaneously.
检测乙型
detection of hepatitis b
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