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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
Metabolizable 111in chelate conjugated anti-idiotype monoclonal antibody for radioimmunodetection of lymphoma in mice
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Establishment of internal-image anti-idiotype monoclonal antibodies to a human antibody to lung cancer
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The objective of this study was to establish hybridomas producing anti-idiotype monoclonal antibodies against a human monoclonal antibody (hmAb) 4G12 that reacts strongly with lung squamous cell carcinomas.
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Moreover, an anti-idiotype monoclonal antibody was produced which was found to bind to 9BG5 and to inhibit its interaction with HA3.
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| | BALB/c mice were immunized with viable hybridoma cells of 5A 8secreting specific McAb (Ab1) against human gl(?)oma and a hybridoma cell line secreting anti-5A8 idiotype McAb 1D1 (Ab2) was obtained by fusing the murine spleen cells with NS-1 cells It was found that 1D1 could specifical1y react with 5A8, inhibi(?) the binding of 5A8 (Ab1) to target cells and induce the production of the target cel1-specific anti-serum (Ab3 i.e. Ab1') with no exposure to target cells in BALA/c mice.It was also found that Ab3 c... | | 5A_8为抗人胶质瘤相关抗原的单克隆抗体(Ab_1)。用分泌5A_8的杂交瘤细胞免疫BALB/c小鼠,制备抗5A_8独特型的单克隆抗体1 D_1(Ab_2)。研究证明,1D_1可特异地与5A_8结合,抑制5A_8与胶质瘤传代细胞(靶细胞)的反应性,但它对另一株抗胶质瘤单克隆抗体5F_4及多克隆抗血清与靶细胞的反应性均无阻断作用。用1D_1免疫BALB/c小鼠,可诱导出与胶质瘤细胞(靶细胞)产生特异性反应的抗体(Ab_3即Ab′_1)。Ab_3可与5A_8亲和层柱提取的靶抗原相结合,并能阻断靶抗原与~(125)I—5 A_8的反应,因而得出结论,1D_1具有胶质瘤相关抗原的内映像(Ab_2 β)。 | | 文摘来源 | | Dot-ELISA is a simple and fast method to detect anti-idiotype McAb. It has higher susceptibility and uses less sample. The sample dotted on nitrocellulose filter ( NCF ) can be kept for use at least for 1 month under 4℃ without losing activity. Glutaraldehyde can strengthen the conjugation of NCF with protein. | | 用斑点酶联免疫吸附试验检测抗独特型单克隆抗体,方法简便、快速,敏感性高,特异性强,所需样品量少,点样后至少可保存一个月;用戊二醛处理硝酸纤维素膜可以增强蛋白吸附。 | | 文摘来源 | | Hybridoma (D3) secteing monoclonal anti-idiotype antibody against MS1-5 was produced by fusion of sp2/0 myeloma cells and spleen cells of syngeneic mice immunized with the mixture of MSl-5 D3 was identified with a series of experiments inclu-. ding competitive and neutralizing inhibition ELISA. D3 antibody could induce anti-sporozoite antibody when administered into rabbit and BALB/c mice, on the other hand, rabbit anti-sporozoite could react specifically with D3. It was found that D3 could competitively in... | | 本实验用抗疟原虫子孢子混合单抗MS1—5免疫同系小鼠获得一株抗独特型单克隆抗体(D3)。经竞争抑制ELISA、IFA以及放免中和抑制试验等证实:D3能模拟抗原CSP诱导特异性抗子孢子抗体的产生,使兔抗D3具有抗子孢子的抗体活性。反之,兔抗子孢子抗体又具有抗D3的活性,两者的相互结合既能被异种抗子孢子抗体所中和,又能被抗原所竞争。不仅如此,D3杂交瘤细胞表面的Ig还表现出于孢子抗原决定簇的抗体结合活性。表明D3抗体是一株具有抗原内影像的抗独特型抗体,即Ab2β。 | | 文摘来源 | |   | | << 更多相关文摘 |
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