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移行细胞癌细胞株
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  transitional cell carcinoma cell line
     HE BIOLOGICAL CHARACTERISTICS OF A NEWLY ESTABLISHED HUMAN BLADDER TRANSITIONAL CELL CARCINOMA CELL LINE(BLZ-211)
     人膀胱移行细胞癌细胞株BLZ-211的生物学特性
短句来源
     The bladder transitional cell carcinoma cell line, BTT739 from the T739 mouse, was transfected with a plasmid that encoded an enhanced green fluorescence protein (GFP) and the cells stably expressing GFP were selected and subcloned. 1×10 3—1×10 4 GFP-labeled BTT739 cells were injected under the skin of ear of T739 mice.
     通过脂质体介导 gfp表达质粒转移及G418筛选获得了稳定表达 gfp的小鼠膀胱移行细胞癌细胞株BTT GFP。
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  “移行细胞癌细胞株”译为未确定词的双语例句
     Objective:To explore the synergism of 4-HPR and adriamycin on the inhibiting growth and inducing apoptosis of human urinary bladder cancer cell li ne(T24).
     目的:观察4-羟苯维胺脂(4-HPR)联合阿霉素对人源性膀胱移行细胞癌细胞株T24的生长抑制和凋亡诱导作用,探讨两者是否存在协同作用。
短句来源
     ESTABLISHMENT AND STUDY OF BIOLOGIC PROPERTIES OF MOUSE BLADDER
     小鼠膀胱移行细胞癌细胞株(WYH929)的建立及其生物学特性
短句来源
     2. A real-time fluorescence quantitative RT-PCR assay using intercalation dye SYBR Green ⅠDye was established and the hTERT mRNA expression in T24 and IMR90 cells was quantified.
     1. 培养端粒酶阳性表达的T24人膀胱移行细胞癌细胞株和端粒酶阴性表达的IMR90人成纤维细胞株。 2. 建立DNA结合染料(SYBR Green Ⅰ)实时荧光定量RT-PCR检测人端粒酶亚单位hTERT mRNA表达的方法,定量检测T24和IMR90细胞株端粒酶亚单位hTERT mRNA的表达。
短句来源
     The antitumor efficacy was evaluated using tumor growth curve and tumor weight. E-J and BIU-87 cells were cultured in vitro, and exposed to 3 μ g/ml concentration of MT-12. HE staining, Hoechst 33342 staining, transmission electronmicroscope were used to determine the apoptotic morphological changes. Apoptotic rates were quantified by flow cytometry(FCM) using Annexin-V FITC/PI dual staining method.
     体外培养人膀胱移行细胞癌细胞株E-J和BIU-87,终浓度为3μg/ml的MT-12分别与贴壁生长的两细胞株作用一定时间后,HE染色、Hoechst33342染色、透射电镜观察凋亡细胞的形态学改变,Annexin V-FITC/PI双染流式细胞术检测凋亡率,琼脂糖凝胶电泳观察细胞凋亡的DNA片段。
短句来源
     The cytotoxicity of the cisplatin,vincristine and 5 fluorouracil on the three sub clonal human bladder transitional cell carcinoma cell lines (BLZ211,BLS211 and BLX211) derived from an untreated patient and BIU 87 bladder cancer cell line was evaluated using the MTT assay.
     用未经化疗的同一膀胱癌手术标本建立的三株膀胱癌细胞株 (BL Z2 11、BL S2 11、BL X2 11)和人膀胱移行细胞癌细胞株 BIU- 87进行了氟尿啶啶 (5- Fu)、顺氯氨铂 (CDDP)、和长春新碱 (VCR)的敏感性试验。
短句来源
更多       
  相似匹配句对
     HE BIOLOGICAL CHARACTERISTICS OF A NEWLY ESTABLISHED HUMAN BLADDER TRANSITIONAL CELL CARCINOMA CELL LINE(BLZ-211)
     人膀胱移行细胞细胞BLZ-211的生物学特性
短句来源
     ESTABLISHMENT AND STUDY OF BIOLOGIC PROPERTIES OF MOUSE BLADDER
     小鼠膀胱移行细胞细胞(WYH929)的建立及其生物学特性
短句来源
     DNA Chip Screening of Cyclin Genes in Human Urinary Bladder Transitional Cell Carcinoma
     膀胱移行细胞细胞周期相关基因的筛选
短句来源
     p16 gene was amplified in BLZ-211. The results indicate that BLZ211 cell line is different from other correspondingcell lines reported so far in literature.
     结果提示BLZ-211细胞是一个研究人膀胱移行细胞细胞生物学的实用模型。
短句来源
     Study on expression of cell cycle-related genes in subclonal cell lines of human cervical carcinoma
     子宫颈癌细胞亚克隆细胞周期相关基因的研究
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  transitional cell carcinoma cell line
In several in vitro studies, pertussis toxin (PTX) inhibited cell proliferation and cell motility in the human transitional cell carcinoma cell line J82.
      
A transitional cell carcinoma cell line, COLO 232, was derived from a primary urinary bladder tumor in a Caucasian male.
      
We have utilized the transitional cell carcinoma cell line TSU-Pr1 to develop an in vivo experimental model of bladder TCC metastasis.
      
Allogeneic CTLs against a human transitional cell carcinoma cell line and autologous CTLs against a renal cell carcinoma cell derived from a surgical specimen were generated.
      


mouse bladder transitional cell carcinoma cell line(WYH

小鼠膀胱移行细胞癌细胞株(WYH929)是本室建立的小鼠膀胱移行细胞癌可移植动物模型(BTT739)。将瘤组织经机械法剪碎,胰酶消化获得细胞悬液,置于含20%小牛血清的RPMI-1640培养液中,经连续传代培养而建株。光镜和电镜观察显示该细胞株具有来自恶性上皮细胞特征,染色体检查证明其分布广泛,众数为70~76条的超二倍体肿瘤。现上海第一人民医院病理科2000851.3细胞形态观察培养过程中,间隔12h取出培养瓶子倒置相差显微镜下观察细胞出长动态变化。同时使用盖玻片法培养,做HE染色,观察细胞形态学特征。1.4染色体制片取18代,28代生长72h细胞,加入0.25μg/ml秋水仙素3滴。2h后,胰酶消化,离心,再以75μmol/LKCl低渗处理后离心,弃去上清液。以甲醇、水醋酸(3:1)液固定15min,离心后保留1ml固定液,制片Giemas染色。油镜下计数。1.5生长曲线测定取第12代细胞采取重复培养法测定其生长率,每瓶细胞数为5×104/瓶,每隔24h取出1瓶进行细胞计数,绘制生长曲线。1.6 流式细胞仪(FCM)检测取24代细胞,以正常T739系小鼠新鲜膀胱为对照,用美国BeclonDic...

小鼠膀胱移行细胞癌细胞株(WYH929)是本室建立的小鼠膀胱移行细胞癌可移植动物模型(BTT739)。将瘤组织经机械法剪碎,胰酶消化获得细胞悬液,置于含20%小牛血清的RPMI-1640培养液中,经连续传代培养而建株。光镜和电镜观察显示该细胞株具有来自恶性上皮细胞特征,染色体检查证明其分布广泛,众数为70~76条的超二倍体肿瘤。现上海第一人民医院病理科2000851.3细胞形态观察培养过程中,间隔12h取出培养瓶子倒置相差显微镜下观察细胞出长动态变化。同时使用盖玻片法培养,做HE染色,观察细胞形态学特征。1.4染色体制片取18代,28代生长72h细胞,加入0.25μg/ml秋水仙素3滴。2h后,胰酶消化,离心,再以75μmol/LKCl低渗处理后离心,弃去上清液。以甲醇、水醋酸(3:1)液固定15min,离心后保留1ml固定液,制片Giemas染色。油镜下计数。1.5生长曲线测定取第12代细胞采取重复培养法测定其生长率,每瓶细胞数为5×104/瓶,每隔24h取出1瓶进行细胞计数,绘制生长曲线。1.6 流式细胞仪(FCM)检测取24代细胞,以正常T739系小鼠新鲜膀胱为对照,用美国BeclonDickinso?

The cytotoxicity of the cisplatin,vincristine and 5 fluorouracil on the three sub clonal human bladder transitional cell carcinoma cell lines (BLZ211,BLS211 and BLX211) derived from an untreated patient and BIU 87 bladder cancer cell line was evaluated using the MTT assay.4 lines showed different sensitivities to the 3 agents:when the concentrations of the drugs were equal to the concentration calculated in vivo.BLZ211 cells were more sensitive than the other lines to 5 Fu and the differences were statistically...

The cytotoxicity of the cisplatin,vincristine and 5 fluorouracil on the three sub clonal human bladder transitional cell carcinoma cell lines (BLZ211,BLS211 and BLX211) derived from an untreated patient and BIU 87 bladder cancer cell line was evaluated using the MTT assay.4 lines showed different sensitivities to the 3 agents:when the concentrations of the drugs were equal to the concentration calculated in vivo.BLZ211 cells were more sensitive than the other lines to 5 Fu and the differences were statistically significant (P<0.05),all 4 cell lines were sensitive to CDDP and were not sensitive to VCE.Our results suggest that difference of chemosensitivity not only exists in bladder cancer patients but also occurs in the cell subpopulations from the same patient.The drug sensitivity testing of the clinical samples may have an important effect in chemotherapy.

用未经化疗的同一膀胱癌手术标本建立的三株膀胱癌细胞株 (BL Z2 11、BL S2 11、BL X2 11)和人膀胱移行细胞癌细胞株 BIU- 87进行了氟尿啶啶 (5- Fu)、顺氯氨铂 (CDDP)、和长春新碱 (VCR)的敏感性试验。结果表明 :四株膀胱癌细胞株对这三种化疗药物的敏感性存在差异。当药物浓度分别为其临床用量的计算值时 ,BL Z2 11细胞株对 5- Fu高度敏感 ,与其它三株细胞的敏感性存在显著差异(P <0 .0 5) ;四株细胞对 CDDP均敏感 ;对 VCR四株细胞均耐药。提示 :对化疗敏感性的差异不仅存在于不同的肿瘤病人 ,而且也存在同一肿瘤标本的不同细胞群体中。因此 ,化疗前进行药敏试验 ,实行用药个体化对提高疗效有重要作用。

The bladder transitional cell carcinoma cell line, BTT739 from the T739 mouse, was transfected with a plasmid that encoded an enhanced green fluorescence protein (GFP) and the cells stably expressing GFP were selected and subcloned. 1×10 3—1×10 4 GFP-labeled BTT739 cells were injected under the skin of ear of T739 mice. On day 2—5 post injection, the most interesting manifestations observed were the chemotaxis-like movement of the tumor cells toward the pre-existing host vasculature, host vessel dilation...

The bladder transitional cell carcinoma cell line, BTT739 from the T739 mouse, was transfected with a plasmid that encoded an enhanced green fluorescence protein (GFP) and the cells stably expressing GFP were selected and subcloned. 1×10 3—1×10 4 GFP-labeled BTT739 cells were injected under the skin of ear of T739 mice. On day 2—5 post injection, the most interesting manifestations observed were the chemotaxis-like movement of the tumor cells toward the pre-existing host vasculature, host vessel dilation and tortuosity and increased extravasation. On day 10 or later, the sprout from pre-existing host vasculature was observed. Once angiogenesis was triggered on, the tumor cells grew more rapidly and exhibited a specific growth pattern where tumor cells always associated with or surrounded the vessels. The newly formed microvessels always showed heavy extravasation. Immunohistochemistry staining revealed strong VEGF and VEGFR2 (Flk-1) expression in tumor cells. Angiography using Rhodamin-labeled dextran showed neovascularization with unprecedented clarity. However, the tumor mass, even bigger than 2 mm and being neovascularized, shrunk and then disappear in 3—5 days and left only delicated host vessels and recovered extravasation. The evidence from this observation indicated that angiogenesis induced by tumor cells after implantation into the host begins at very early stage. The micrometastases foci could not form or survive without vigorous and continuous angiogenesis. Furthermore, there was active VEGF paracrine and autocrine expression in tumor and high level VEGF secretion by tumor cells plays an important role in initiating angiogenesis and supporting micrometastases.

通过脂质体介导 gfp表达质粒转移及G418筛选获得了稳定表达 gfp的小鼠膀胱移行细胞癌细胞株BTT GFP。利用 gfp作为肿瘤细胞的标记 ,结合罗丹明标记的葡聚糖尾静脉注射作血管造影 ,建立了一种新的肿瘤模型 ,具有简便、可靠、无创伤的优点 ,特别是可以通过荧光显微镜动态观察肿瘤转移病灶形成最早期阶段肿瘤细胞生长与肿瘤局部宿主血管的变化。利用新型鼠耳肿瘤模型观察到移植的肿瘤细胞会主动向宿主血管迁移 ,当肿瘤生长至仅 0 .3mm直径大小时即可见血管生成。免疫组织化学染色观察到肿瘤内新生血管SMA及CD31染色阳性 ;肿瘤细胞不仅高水平表达VEGF ,也高水平表达VEGF的受体Flk 1。提示肿瘤局部存在VEGF自分泌与旁分泌通路 ,肿瘤细胞高水平表达VEGF是新生血管生成的主要原因

 
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