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框架编码
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  frame coding
     To explore the structure and function of human glycosylphosphatidylinositol-specific phospholipase D(GPI-PLD) cDNA, a GPI-PLD cDNA with the size of approximately 2.6 kb was cloned from human bone marrow stromal cells using RT-PCR. This target gene had a complete open reading frame coding a signal peptide of 23 amino acids and a mature peptide of 817 amino acids.
     为探讨人糖基化磷脂酰肌醇特异性磷脂酶D(GPI PLD)cDNA的结构及功能 ,应用RT PCR从人骨髓基质细胞中克隆了长约 2 6kb的GPI PLDcDNA ,包含完整阅读框架 ,编码 2 3个氨基酸的信号肽及 817个氨基酸的成熟肽 .
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  “框架编码”译为未确定词的双语例句
     Results The complete genome of DEN2-FJ11 strain was composed of 10 723 nucleotides, it included single open reading frame, which encodes 3 391 amino acids.
     结果 DEN2 FJ11株基因组全长 10 72 3个核苷酸 ,含有 1个单一的读码框架 ,编码 3391个氨基酸。
短句来源
     The CiEPV and GsEPV spheroidin genes respectively harbored ORFs of 2 922 bps and 2 967 bps that were capable of coding polypeptides of 109.2 and 111.1 kDa.
     CiEPV与GsEPV包涵体蛋白基因分别包含2922bps,2967bps的开放阅读框架,编码109.2kDa,111.1kDa蛋白质。
短句来源
     The cDNA was 567 bp in length with open reading frame of 188 amino acids.
     经序列分析,此cDNA为一个567 bp长的开放阅读框架,编码由188个氨基酸组成的病毒外壳蛋白。
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     Its cDNA open reading frame encodes 1765 amino acids.
     其cDNA开放阅读框架编码1765个氨基酸。
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     The HA gene was 1 745 bp in length and coded for 566 amino acids.
     测序结果表明,该毒株的HA基因全长为1745bp,含有完整的阅读框架,编码566个氨基酸;
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  相似匹配句对
     3. Two extensions to the coded cooperation framework are presented.
     3.提出了两个现存的编码框架
短句来源
     Its cDNA open reading frame encodes 1765 amino acids.
     其cDNA开放阅读框架编码1765个氨基酸。
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     1 encoding for RAS and call signalling messages.
     1编码;
短句来源
     DIGITAL CODING OF SPEECH SIGNALES
     语音编码
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     The Necessary Conditions for Frames
     框架的必要条件
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  frame coding
A product of alternative splicing contained an open reading frame coding for the cytoplasmic portion of LTβ.
      
Comparison of the Tvv1 open reading frame coding potential with those of drosophila copia and tobacco Tnt1, revealed that Tvv1 is closely related to Ty1 copia-like retrotransposons.
      
Starting from an ATG initiator codon, an open reading frame coding for 213 amino acids was found.
      
vannameiactinT2 cDNA has a 1,131-bp open reading frame coding for 377 amino acids.
      
We cloned and sequenced the large continuous open reading frame coding for the salamander Pleurodeles waltl RAG1 protein.
      
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On the basis of the nucleic acid sequence of cDNA encoding an aspartic proteinase inhibitor,two primer were synthesized. Using the potato genomic DNA as a template,an aspartic proteinase inhibitor gene was synthesized by PCR. The gene was inserted into the SmaI site of pUC18 for constructing pAPI. The results of dot hybridization and sequence analysis showed that the pAPI contains the region encoding an aspartic proteinase inhibitor. An open reading frame of the gene encodes a precursor protein of 221 amino...

On the basis of the nucleic acid sequence of cDNA encoding an aspartic proteinase inhibitor,two primer were synthesized. Using the potato genomic DNA as a template,an aspartic proteinase inhibitor gene was synthesized by PCR. The gene was inserted into the SmaI site of pUC18 for constructing pAPI. The results of dot hybridization and sequence analysis showed that the pAPI contains the region encoding an aspartic proteinase inhibitor. An open reading frame of the gene encodes a precursor protein of 221 amino acids. The genomic DNA sequence shares 94. 3% nucleotide and 90% amino acid identity with cDNA pudlished before. There are no nitron sequences interupting the coding region.

以马铃薯基因组DNA为模板并基于天冬氨酸蛋白酶抑制剂基因的cDNA序列所设计的两个引物,通过PCR扩增得到666bp的天冬氨酸蛋白酶抑制剂基因编码区,并将此片段克隆到pUC18的SmaI位点.序列分析结果表明该基因除去末端一终止密码子外其可译框架编码221个氨基酸残基,前导肽包括32个氨基酸残基,故该抑制剂的成熟蛋白由189个氨基酸组成,第67位的精氨酸为抑制胰蛋白酶的活性中心.与cDNA相比核苷酸的同源性为94.3%,氨基酸的同源性为90%.基因DNA无内含子.

Oxidatioin reduction reactions of oestrogens and androgens at position C 17 are catalysed by 17β hydroxysteroid dehydrogenases (17β HSDs). cDNA clones for mouse 17β HSD Ⅲ were isolated from mouse testis using Race. The largest cDNA contained 1131 nucleotides, consisting of a short 5′ noncoding segment, a coding segment of 918 nucleotides begun by a ATG initiating codon and terminated by a TAG codon, and a 155 nucleotide long 3′ noncoding segment. The open reading frame encoded a polypeptide of 305 amino acid...

Oxidatioin reduction reactions of oestrogens and androgens at position C 17 are catalysed by 17β hydroxysteroid dehydrogenases (17β HSDs). cDNA clones for mouse 17β HSD Ⅲ were isolated from mouse testis using Race. The largest cDNA contained 1131 nucleotides, consisting of a short 5′ noncoding segment, a coding segment of 918 nucleotides begun by a ATG initiating codon and terminated by a TAG codon, and a 155 nucleotide long 3′ noncoding segment. The open reading frame encoded a polypeptide of 305 amino acid residues. 17β HSD Ⅲ mRNA was expressed specially in testis. This study provides the basis for a better understanding of the molecular mechanisms involved in 17β HSD deficiency and sex steroid metabolism.

17β-羟甾脱氢酶(17β-HSD)催化17-酮类固醇与17β-羟类固醇相互转换。本研究从小鼠睾丸中纯化出小鼠Ⅲ型17β-HSD的cDNA克隆,最长的cDNA核苷酸数为1131,包含一个短的5′非编码序列、918个核苷酸编码序列和155个核苷酸3′非编码序列;其起始密码子为ATG,终止密码子为TAG,开读框架编码为305个氨基酸的多肽。该研究为进一步了解17β-HSD的缺乏和性激素代谢的分子机理奠定了基础。

AIM:To clone and analyze the c DNA encoding immunodiagnostic antigen of cysticerco- sis.METHODS:Immunodiagnostic antigens of cysticercosis were obtained studied using re- combinant DNA techniques.c DNA was synthesized from m RNA and aλgt1 1 expression li- brary was constructed and immunoscreened with human and/ or pig cysticercosis sera.RE- SUL TS:Four positive c DNA clones(λc C1、λc C2、λc H1 andλc P1 ) were isolated.Theλc C1 c DNA was 1 0 70 bp in length and consisted of a single open reading frame.The...

AIM:To clone and analyze the c DNA encoding immunodiagnostic antigen of cysticerco- sis.METHODS:Immunodiagnostic antigens of cysticercosis were obtained studied using re- combinant DNA techniques.c DNA was synthesized from m RNA and aλgt1 1 expression li- brary was constructed and immunoscreened with human and/ or pig cysticercosis sera.RE- SUL TS:Four positive c DNA clones(λc C1、λc C2、λc H1 andλc P1 ) were isolated.Theλc C1 c DNA was 1 0 70 bp in length and consisted of a single open reading frame.The open reading frame of747bp encoded a polypeptide of2 49amino acids with a molecular weight of2 7.36 k Da.Theλc C1 fusion protein was identified as an common antigen for immunodiagnosis of both human and pig cysticercosis.CONCLUSION:Theλc C1 fusion protein was highly sensi- tive and specific as compared to crude somatic antigen for the immunodiagnosis of cysticerco- sis.

目的 :克隆与分析囊虫病诊断用抗原及其编码基因。方法 :采用基因重组技术 ,构建来源于猪囊尾蚴 m RNA的 c DNA文库。以囊虫病患者、囊虫病病猪的血清为探针从 c DNA文库中筛选出目的克隆。结果 :获得人、猪共同抗原 2个 (分子量分别为 2 8k Da和 18k Da) ,人特异性抗原和猪特异性抗原各 1个 (分子量为 34 k Da、 14k Da)。这些抗原的编码克隆分别命名为λc C1、λc C2、λc H1及 λc P1。c C1c DNA含有编码 2 8k Da的人、猪共同抗原的完整基因 ,全长 10 70 bp,起始密码 ( ATG)从自 2 2 bp,终止密码 ( TGA)结于 74 7bp,长为 74 7bp的开放阅读框架编码由 2 4 9个氨基酸组成的多肽 ,其理论推导分子量为 2 7.36k Da。结论 :以 c C1等融合蛋白为诊断用抗原 ,具有高度的特异性和较理想的敏感性。

 
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