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细胞胞浆酶
相关语句
  cytosolic enzyme
     Effect of Selective Head Cooling on Cytosolic Enzyme following Cerebral Complete Ischemia in Rabbits
     头部重点低温对完全性缺血后兔脑细胞胞浆酶变化的影响
短句来源
     Objective To study the changes of serum cytosolic enzyme in rat with cerebral ischemia.
     目的 研究大鼠脑缺血时脑细胞胞浆酶含量变化的影响。
短句来源
  “细胞胞浆酶”译为未确定词的双语例句
     Methods The release of cytoplasm enzyme,lactate dehydrogenase(LDH),lipid peroxidation product,malondialdehyde(MDA),and anti peroxidant,glutathione peroxidase(GSH Px),were assayed in primary cultured keratinocytes treated by UVA and TPP. Cytometry and Flow Cytometer were used to count the viable cells and the cell kinetics,too.
     方法 在原代培养大鼠皮肤角朊细胞基础上 ,经UVA照射后 ,测定角朊细胞胞浆酶———乳酸脱氢酶 (LDH)释放情况 ,脂质过氧化产物丙二醛 (MDA)、谷胱甘肽过氧化物酶 (GSH Px)水平 ,并测定培养细胞的存活率和细胞周期动力学。
短句来源
     Objective:To study the changes of serum cytosolic enzymes in rats after cerebral ischemia/reperfusion.
     目的 :研究大鼠脑缺血灌流时脑细胞胞浆酶含量的变化及其临床意义。
短句来源
  相似匹配句对
     Cells had plenty of plasma.
     细胞胞浆丰富;
短句来源
     Positive cells were stained with cytoplasm.
     阳性细胞胞浆着色。
短句来源
     Telomerase and Cell Aging
     端粒细胞衰老
短句来源
     Studies on the B-Cell Epitopes of L-asparaginase
     L-天冬酰胺B细胞表位研究
短句来源
     S100, LZM are stain at cytoplasm.
     S100、溶菌细胞胞浆着色。
短句来源
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  cytosolic enzyme
Cell viability was assessed by the activity of the cytosolic enzyme lactate dehydrogenase (LDH) liberated into the culture medium and by trypan blue exclusion.
      
Digoxin in a concentration of 1.28 μmol/l produced ventricular fibrillation and an extensive decrease of the myocardial ATP content, followed by the release of great activities of the cytosolic enzyme creatine kinase (CK).
      
Additionally, a wide-ranging reprograming of the cellmembrane involving ion-channels, receptors, ligands and the translocation of cytosolic enzyme systems is probably necessary to achieve myocardial protection.
      
We also determined the levels of acylphosphatase, a cytosolic enzyme, that could play a regulatory effect on SR Ca2+ pump by hydrolyzing the phosphorylated intermediate of this transport system.
      
DDC is generally considered to be a cytosolic enzyme.
      
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Ischemia reperfusion injury(IRI) model of rat heart was prepared by preperfusion for 15 min, then a suspension for 45 min and recycling reperfusion for 15 min with 30 ml KH buffer. The leakage of lactate dehydrogenase(LDH), protein, myoglobine and nitrite (NO - 2) in the circular perfusion fluid were measured. Myocardial nitric oxide synthase(NOS) activity and L arginine transport were observed. In the IR group,the leakage of LDH, protein, myoglobine and NO - 2 were increased respectively by 4 1, 5 4,...

Ischemia reperfusion injury(IRI) model of rat heart was prepared by preperfusion for 15 min, then a suspension for 45 min and recycling reperfusion for 15 min with 30 ml KH buffer. The leakage of lactate dehydrogenase(LDH), protein, myoglobine and nitrite (NO - 2) in the circular perfusion fluid were measured. Myocardial nitric oxide synthase(NOS) activity and L arginine transport were observed. In the IR group,the leakage of LDH, protein, myoglobine and NO - 2 were increased respectively by 4 1, 5 4, 1 and 1 2 times( P <0 01) and NOS(tNOS, iNOS, cNOS) activity by 48 2%, 43 2%and 52 1%, ( P <0 01, respectively) as compared with the control group. L arginine transport might be mediated by either high or low affinity transport system in cardiac tissue. In the IR group, L arginine transport increased significantly with the V max being increased by 48% and 2 times respectively for the low affinity and the high affinity transport as compared with control. Michaelis constant (km) was decreased by 47 4% for low affinity transport ( P <0 05), but not significantly changed for the high affinity transport. These results suggest that the increase of nitric oxide generation might result from the increased myocardial NOS activity and L arginine transport during IRI.

为探讨大鼠心脏缺血再灌注损伤(IRI)期间一氧化氮(NO)生成增加的环节和过程。本实验用离体灌流大鼠心脏,预灌流15min,停灌45min,取30mlKH液循环灌流15min,观察冠脉流出液中细胞胞浆酶(LDH)、蛋白质、肌红蛋白漏出量和NO-2含量、心肌组织NOS活性、L精氨酸(LArg)转运的改变。结果显示,心脏IRI后,冠脉流出液中LDH活性、蛋白质和肌红蛋白量较对照组分别增加41,54和1倍(均P<001)。NO-2含量增加12倍(P<001)。心肌组织tNOS活性、iNOS活性和cNOS活性分别增加482%、432%和521%(均P<001)。NO-2含量与心肌组织iNOS活性呈正相关,(r=07942,P<001)。心肌组织LArg转运呈现高、低亲和两种方式。IRI组心肌LArg转运能力增强,最大转运速率(Vmax)较对照组升高48%(低亲和,P<005)和2倍(高亲和,P<001);低亲和Km值降低474%(P<005),高亲和Km值改变无统计学意义。LArg转运高、低亲和转运载体的Vmax与iNOS活性、NO-2含量均呈高度正相关关系。结果...

为探讨大鼠心脏缺血再灌注损伤(IRI)期间一氧化氮(NO)生成增加的环节和过程。本实验用离体灌流大鼠心脏,预灌流15min,停灌45min,取30mlKH液循环灌流15min,观察冠脉流出液中细胞胞浆酶(LDH)、蛋白质、肌红蛋白漏出量和NO-2含量、心肌组织NOS活性、L精氨酸(LArg)转运的改变。结果显示,心脏IRI后,冠脉流出液中LDH活性、蛋白质和肌红蛋白量较对照组分别增加41,54和1倍(均P<001)。NO-2含量增加12倍(P<001)。心肌组织tNOS活性、iNOS活性和cNOS活性分别增加482%、432%和521%(均P<001)。NO-2含量与心肌组织iNOS活性呈正相关,(r=07942,P<001)。心肌组织LArg转运呈现高、低亲和两种方式。IRI组心肌LArg转运能力增强,最大转运速率(Vmax)较对照组升高48%(低亲和,P<005)和2倍(高亲和,P<001);低亲和Km值降低474%(P<005),高亲和Km值改变无统计学意义。LArg转运高、低亲和转运载体的Vmax与iNOS活性、NO-2含量均呈高度正相关关系。结果提示:心脏?

Objective To study the effects of UVA and tea polyphenols(TPP,a kind of polyphenol substances from a special plant) on lipid peroxidation and cell kinetics in primarily cultured rat skin keratinocytes. Methods The release of cytoplasm enzyme,lactate dehydrogenase(LDH),lipid peroxidation product,malondialdehyde(MDA),and anti peroxidant,glutathione peroxidase(GSH Px),were assayed in primary cultured keratinocytes treated by UVA and TPP.Cytometry and Flow Cytometer were used to count the viable cells and the...

Objective To study the effects of UVA and tea polyphenols(TPP,a kind of polyphenol substances from a special plant) on lipid peroxidation and cell kinetics in primarily cultured rat skin keratinocytes. Methods The release of cytoplasm enzyme,lactate dehydrogenase(LDH),lipid peroxidation product,malondialdehyde(MDA),and anti peroxidant,glutathione peroxidase(GSH Px),were assayed in primary cultured keratinocytes treated by UVA and TPP.Cytometry and Flow Cytometer were used to count the viable cells and the cell kinetics,too. Results UVA could obviously improve the release of LDH from 827.55 U/L to 1 312.47 U/L,increase the formation of peroxidation product MDA,decrease the level of GSH Px and reduce the survival rate of cells and the cell proliferative index(PI) from 34.24% to 17.98%.TPP could significantly protect and inhibit the damage induced by UVA. Conclusion UVA could damage skin keratinocytes,and TPP could protect keratinocytes from damage induced by UVA.The result could provide useful information in developing natural anti violet product.

目的 探讨长波紫外线 (UVA)对原代培养的大鼠皮肤角朊细胞脂质过氧化和生长状况等影响 ,同时探讨一种植物多酚———茶多酚 (TPP)在此过程中所起的作用。方法 在原代培养大鼠皮肤角朊细胞基础上 ,经UVA照射后 ,测定角朊细胞胞浆酶———乳酸脱氢酶 (LDH)释放情况 ,脂质过氧化产物丙二醛 (MDA)、谷胱甘肽过氧化物酶 (GSH Px)水平 ,并测定培养细胞的存活率和细胞周期动力学。结果 UVA可以引起体外培养的角朊细胞膜通透性增强 ,胞浆酶LDH释放增加 (从 82 7.5 5U/L增至 1312 .47U/L) ;脂质过氧化产物MDA水平升高 ,抗氧化酶GSH Px水平降低 ;细胞存活率下降 ,细胞周期动力学表现为细胞增殖抑制 :增殖指数 (PI)从 34.2 4%降至 17.98%。天然提取物TPP(质量浓度为 0 .1% )可以比较明显地抑制UVA引起的上述损害。结论 UVA可损伤原代培养的大鼠皮肤角朊细胞 ,TPP为天然防晒产品的开发提供理论依据。

Objective To study the changes of serum cytosolic enzyme in rat with cerebral ischemia.Methods Autobiochemical analyzer was used to measure the patients' creatine kinase (CK) of serum cytosolic enzyme, creatine kinase of brain band isoenzyme (CKBB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST).Results In the 20min ischemia group, the activities of serum CK, CKBB and LDH were significantly higher than in the shamoperated group (P<0.05 or 0.01), and in 60min and 120min ischemia groups the...

Objective To study the changes of serum cytosolic enzyme in rat with cerebral ischemia.Methods Autobiochemical analyzer was used to measure the patients' creatine kinase (CK) of serum cytosolic enzyme, creatine kinase of brain band isoenzyme (CKBB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST).Results In the 20min ischemia group, the activities of serum CK, CKBB and LDH were significantly higher than in the shamoperated group (P<0.05 or 0.01), and in 60min and 120min ischemia groups the activities of CK, CKBB, LDH and AST were all greatly increased in contrast with the shamoperated group (P<0.01).Conclusion The activities of cytosolic enzyme are significantly increased during cerebral ischemia and can be used to detect the severity of the injured cell membrane.

目的 研究大鼠脑缺血时脑细胞胞浆酶含量变化的影响。方法 采用全自动生化分析仪检测血清肌酸激酶 (CK)、肌酸激酶脑型同工酶 (CK-BB)、乳酸脱氢酶 (L DH)以及天门冬氨酸氨基转换酶 (AST)的含量。结果 大鼠脑缺血 2 0 min,血中 CK、CK-BB和 L DH的含量明显高于假手术组 (P<0 .0 5或 P<0 .0 1) ;脑缺血 6 0 min和 12 0 min,血中 CK、CK-BB、LDH和 AST的含量均明显高于假手术组 (P<0 .0 1)。结论 脑缺血时血中胞浆酶的活性增加 ,可用来判断细胞膜受损伤的程度

 
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