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In order to dermine the effect of peenteral nutrition (PN), dexamethson (DM) and theircombination(PN+DM) on lung cancer. Thirty six nude mice bearing human adenocarcinoma of thelung cameinto ewperiments. The alterations of DNA synthesis phase in tumor cells were detected withFCM method. the 24hr of administration, percentage of S phase cells (S%) were53. 4± 10. 2,46. 5± 9. 1,and 59. 8±8. 0 for PN,DM and PN+DM respectively. In the control group it was 44. 7± 8. 1. After 48hr,S% in the above three groups were... In order to dermine the effect of peenteral nutrition (PN), dexamethson (DM) and theircombination(PN+DM) on lung cancer. Thirty six nude mice bearing human adenocarcinoma of thelung cameinto ewperiments. The alterations of DNA synthesis phase in tumor cells were detected withFCM method. the 24hr of administration, percentage of S phase cells (S%) were53. 4± 10. 2,46. 5± 9. 1,and 59. 8±8. 0 for PN,DM and PN+DM respectively. In the control group it was 44. 7± 8. 1. After 48hr,S% in the above three groups were 36. 4± 4. 8' 39. 9± 6. 6,and 46, 6± 6. 9,respectively. In the control group it was 44. 4±8. 8. The results showed that S% in PN+DM groupat 24hr was increased significantly(P<0. 05) compeing with thase in the control group and suggestedthat the PN+DM combination at 24hr post-administration might have synergistic effect on the ratioof tumor cells at S phase,This alterated cytokinetics might influence the chemosensitivity of tumorcells to cycle-specific agents. 采用胃肠外营养(PN)、地塞米松(DM)及PN+DM对36只人肺腺癌裸鼠模型LAX-83肿瘤细胞中中期细胞百分率(S%)的影响,PN组予12.54kJ/kg-1·d-1+氮量0.2g/kg-1·d-1,DM组予20μg/d,对照组予生理盐水,分2次早晚腹腔注射,用FCM测定S%,结果,24h,PN+DM组的S%增加最大,显著高于对照组,提示PN+DM的联合作用。24h后对S%增加有协同作用。 Objective: To study the inducible expression of hepatitis C virus ns3 gene (HCV ns3) in eukaryotic cells. Methods: The ns3 gene was obtained from plasmid pBns3 by polymerase chain reaction and inserted into the cloning vector pGEMT. Then, the ns3 was subcloned into the vector pMSG to generate dexamethasone (DM)inducible expression plasmid pMSGns3. CHO cells were transfected by pMSGns3 by using calcium phosphate precipitated method and cultivated for 12 to 24 h.The transfected cells were induced... Objective: To study the inducible expression of hepatitis C virus ns3 gene (HCV ns3) in eukaryotic cells. Methods: The ns3 gene was obtained from plasmid pBns3 by polymerase chain reaction and inserted into the cloning vector pGEMT. Then, the ns3 was subcloned into the vector pMSG to generate dexamethasone (DM)inducible expression plasmid pMSGns3. CHO cells were transfected by pMSGns3 by using calcium phosphate precipitated method and cultivated for 12 to 24 h.The transfected cells were induced with DM and the transient expression of NS3 protein was analyzed by using ELISA and Westernblot methods. Results: After treatment with 3×10~(-8) mol/L DM,the expression of NS3 was observed in the transfected CHO cells. A slightly higher level of NS3 was shown with the delay time of DM treatment. Conclusion: The inducible expressing vector pMSGns3 might be helpful for further studies of the characterizations of the ns3 gene in vivo. 目的:研究丙肝病毒非结构蛋白基因3(ns3基因)在体外真核细胞中的诱导表达。方法:以PCR方法从含HCVns3基因的重组载体pBns3中扩增出ns3基因,并将其插入到克隆载体pGEM-T中,再与表达载体pMSG重组,以得到重组表达载体pMSG-ns3。然后采用磷酸钙沉淀法将其转染哺乳动物CHO细胞,经地塞米松(DM)诱导,采用ELISA及Western-blot技术检测NS3蛋白的表达水平。结果:用所构建的诱导型表达载体pMSG-ns3转染CHO细胞,经DM(3×10-8mol/L)诱导后可表达NS3蛋白,其浓度与时间呈正相关。结论:所得到的表达载体在培养细胞中能够有效地表达,为下一步研究丙肝病毒ns3基因在活体内的生物学特性准备了条件 Objective: 目的:研究哮喘时嗜酸性粒细胞(Eos)凋亡与Fas表达的关系及药物对它们的影响。方法:应用地塞米松(DM)、雷公藤甲素(Triptolide,TP)及氨茶碱(AM)干预哮喘豚鼠,以TUNEL法检测细胞凋亡,以RT-PCR及原位杂交检测FasmRNA表达。结果:哮喘组Eos凋亡率较正常对照组显著降低(P<0.01),应用DM、TP、AM后均显著增加(P<0.01)。正常豚鼠Eos可检测到FasmRNA表达,不同密度Eos表达差异不显著(P>0.05)。哮喘组FasmRNA明显减少,以低密度Eos减少明显(P<0.05),应用DM、TP、AM后其表达明显增加。结论:凋亡调节的缺失是导致组织及血中Eos增多的重要原因,Fas抗原参与了哮喘Eos凋亡的调节,低密度Eos更能反映哮喘的特征。DM、TP、AM可促进哮喘肺组织中的Eos表达Fas抗原,进而加强细胞凋亡。
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