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The levels of guanidino-compounds in the plasma of patients with various liver diseases were determined by high-performance liquid chromatography using benzoin as a pre-column fluorescent derivatization reagent. The results showed that the levels of guanidinoacetic acid (GAA) in plasma were significantly higher in patients with liver cirrhosis than those in normal human and other liver diseases, and that the levels of other guanidino -compounds in plasma of patients with various liver diseases were not significantly... The levels of guanidino-compounds in the plasma of patients with various liver diseases were determined by high-performance liquid chromatography using benzoin as a pre-column fluorescent derivatization reagent. The results showed that the levels of guanidinoacetic acid (GAA) in plasma were significantly higher in patients with liver cirrhosis than those in normal human and other liver diseases, and that the levels of other guanidino -compounds in plasma of patients with various liver diseases were not significantly changed . There was no significant relationship between the levels of guanidino-compounds and common indicators for liver function in patients with various liver diseases. 作者采用安息香为柱前荧光衍生剂的高效液相层析法检测几种肝病患者血浆中胍类化合物的含量。结果表明,血浆中胍基乙酸在肝硬化等重症肝病时显著升高,在其它肝病时未见明显变化;血浆中其它胍类化合物在几种肝病时均未见明显的改变。肝病时血浆胍类化合物的变化与其它常见肝功能指标的变化没有显著相关性。 Intrahepatic hepatitis C virus antigen (HCAg) was detected in liver tissue of 174 patients with chronic hepatitis (CH) ,severe hepatitis (SH) and hepatocellular -carcino ma (HCC) by direct enzyme-labelled technique. The detection rate was 10. 71%, 8. 10%, 2. 86% respectively.suggesting that there exist HCV infection in patients with chronic and severe liver diseases. The relationship between HCV and HBV infection was also studied and no significant difference was found in terms of HCAg detection rate between... Intrahepatic hepatitis C virus antigen (HCAg) was detected in liver tissue of 174 patients with chronic hepatitis (CH) ,severe hepatitis (SH) and hepatocellular -carcino ma (HCC) by direct enzyme-labelled technique. The detection rate was 10. 71%, 8. 10%, 2. 86% respectively.suggesting that there exist HCV infection in patients with chronic and severe liver diseases. The relationship between HCV and HBV infection was also studied and no significant difference was found in terms of HCAg detection rate between intrahepatic HBV positive and negative cases. It seems that HCV infection was unlikely related to HBV infection. 采用直接酶标记法对174例慢性肝炎、重症肝炎和肝癌组织中的HCV抗原进行了检测,HCV 抗原的检出率分别为10.71%、8.10%和2.68%。表明在我国慢性和重症肝病中存在HCV的感染。通过对HCV与HBV 感染关系的研究,发现肝内HBV 标志阳性和阴性者HCV抗原的检出率无明显差异,说明HCV感染与HBV感染之间似无明显关系。 PURPOSE To establish a radioimmunoassay method for the measurement of human Plasma protein C(PC).METHODS PC was isolated and purified from human plasma.The antisera agsinst PC was obtained by immunizing rabbits. Iodination of PC was carried out with chroramine-T.RESULTS The sensitivity was 3.94μg/L,and the assay covered 6.25-1024μg/L for PC.The intra-assay and inter-assay CV were 4.4% and 9.68% respectively,with a recovery rate of 104.28%.There was no cross reaction with factor Ⅱ.The normal value was 3.84±0.34mg/L... PURPOSE To establish a radioimmunoassay method for the measurement of human Plasma protein C(PC).METHODS PC was isolated and purified from human plasma.The antisera agsinst PC was obtained by immunizing rabbits. Iodination of PC was carried out with chroramine-T.RESULTS The sensitivity was 3.94μg/L,and the assay covered 6.25-1024μg/L for PC.The intra-assay and inter-assay CV were 4.4% and 9.68% respectively,with a recovery rate of 104.28%.There was no cross reaction with factor Ⅱ.The normal value was 3.84±0.34mg/L in 36 normal persons.Value of 1.03±0.4mng/L was found in 16 patients with fulminating hepatitis complicated with coagulation distubance.CONCLUSION It is an effective apporach for the diagnosis of hereditary or acquired PC deficiency and also for the study of thrombotic diseases. 目的:建立人血浆蛋白C(PC)放射免疫分析法(RIA),并初步应用于临床。方法:从人血浆中分离和纯化PC,免疫新西兰家兔,获得特异性抗血清,氯胺T法制备125I-PC,用平衡法建立PCRIA。结果:方法灵敏度为394μg/L,工作范围为6.25—1024μg/L,批内和批间CV分别为4.40%和9.68%,回收率为104.28%,与因子Ⅱ未见交叉反应。测定36例正常人血浆PC含量为3.84±0.34mg/L,16例重症肝病伴发凝血障碍患者为1.03±0.41mg/L。结论:可为遗传性或后天性PC缺乏疾病的诊断及血栓病的研究提供一种有效的手段。
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