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-核苷酸
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  5 '-nucleotide
     The 5'-nucleotide in lentinus edodes were identified as 5'-inosine monophosphate, 5'-guanosine monophosphate, 5'-uridine monophosphate and 5'-adenosine monophosphate by using HPLC and LC/MS.
     以HPLC与LC/MS联合定性香菇中5'-核苷酸为5'-肌苷酸,5'-鸟苷酸,5'-尿苷酸和5'-腺苷酸。
短句来源
     In extracted ferment with poly-nucleotide water takes up less than 6%,nitrogen over 6% and 5'-nucleotide over 20%.
     高核苷酸酵母精,水分6%以下,总氮6%以上,5'-核苷酸20%以上;
短句来源
     Analysis of 5'-Nucleotide in Lentinus Edodes with High Performance Liquid Chromatography-Mass Spectrometry
     香菇中5'-核苷酸的高效液相色谱-质谱分析
短句来源
     A new high performance liquid chromatography/mass spectrometric method for the analysis of 5'-nucleotide in lentinus edodes was reported.
     采用高效液相色谱/质谱法,对香菇中5'-核苷酸进行了分析。
短句来源
     (2) higher pH did not improve the 5'-nucleotide contents even in the presence of RNase from malted barleyroots, but at the pH 8.0, the addition of Acalase could increase the final yield, soluble protein contents and 5’-nucleotide inthe presence of RNase from malted barley roots.
     在pH8自溶的产物中5’-核苷酸和3’-核苷酸的量均显著低于在pH5自溶的产物中的量。 酵母在pH8~8.5条件下自溶时,外加蛋白酶可以显著提高酵母自溶物产物中可溶性固形物含量、可溶性蛋白质和核苷酸的含量,显著降低核酸含量,产物中的5'-核苷酸含量显著比3'-核苷酸含量高。
短句来源
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  5 '-ribonucleotides
     5'-Ribonucleotides is high added value products used in food industries or having important applications in the pharmaceutical industry.
     5'-核苷酸是一种重要核酸类物质,在食品及医药工业等方面都有着广泛的用途。
短句来源
     Till now, enzymatic method is still the only method that can be utilized for industrial production of all four types of 5'-ribonucleotides (5'-CMP, 5'-GMP, 5'-UMP and 5'-AMP).
     目前仅有酶法可用来工业化生产四种5'-核苷酸,它是用核酸酶P_1水解酵母RNA的磷酸二酯键以得到5'-核苷酸的。
短句来源
     In order to change this disadvantageous status, we have developed this item studying on the production of 5'-ribonucleotides by enzymatic hydrolysis method.
     本论文的主要研究内容集中在该项目上游工艺部分,包括核酸酶P_1的发酵以及利用核酸酶P_1催化RNA的水解生产5'-核苷酸(即酶解部分)两部分。
短句来源
  “5'-核苷酸”译为未确定词的双语例句
     STUDY ON THE PROCESS OF 5’-NUCLEOTIDES SEPARATION USING THE CATIONIC EXCHANGE RESIN NH-1
     NH-1分离5'-核苷酸的研究
短句来源
     THE SIGNIFICANCE OF γ-GLUTAMYL TRANSPEPTIDASE (γ-GTP) AND 5'-NUCLEOTIDE PHOSPHODIESTERASE(5'-NPDase) IN THE DIAGNOSIS OF HEPATOCELLULAR CARCINOMA
     r-谷氨酰转肽酶与5'-核苷酸磷酸二酯酶两种同功酶检查对原发性肝癌诊断价值的探讨
短句来源
     DETECTION OF EARLY NEPHRIC DAMAGE FOR USING CIS DICHOLORDIAMINE PLATINUM BY URINARY 5' NUCLEOTIDE PHOSPHODIESTERASE
     尿5'-核苷酸磷酸二酯酶监测顺铂早期肾损害的研究
短句来源
     DETERMINATION OF 5'-NUCLEOTIDES DEGRADED FROM THE RNA OF YEASTS BY RP-HPLC
     反相高效液相色谱法测定酵母RNA降解的5'-核苷酸
短句来源
     And Japanese have succeeded in large-scale production of nucleotides when they grasped this method as early as 1970s.
     日本早在上世纪70年代就用此方法实现了5'-核苷酸的工业化生产。
短句来源
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  5 '-nucleotide
The adult heart depends largely on salvage synthesis to supply its 5'-nucleotide needs.
      
Inhibition of cyclic-3',5'-nucleotide-phosphodiesterase as a possible mode of action of papaverine and similarly acting drugs
      
A highly sensitive method for the determination of cyclic 3',5'-nucleotide phosphodiesterase (PDE) is described.
      
Inhibition by papaverine of 3',5'-nucleotide-phosphodiesterase (E.C.3.1.4.1.) from bovine uterus muscle
      
A cyclic 3',5'-nucleotide phosphodiesterase from rat adrenals was partially purified.
      
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  5 '-ribonucleotides
Monosodium glutamate (MSG) elicits a unique taste in humans called 'umami' that is potentiated synergistically by the 5'-ribonucleotides IMP and GMP.Recent studies suggest that several mechanisms are involved in the transduction of umami taste.
      
2',3'- and 5'-ribonucleotides, it is, however, strongly inhibited by thiourea, guanidine-hydrochloride, and sodium dodecylsulfate, but weakly inhibited by sodium-ethylendiamin-tetraacetate resp.
      
The 5'-ribonucleotides were determined after thin-layer chromatography by measuring the reflectance at the absorption maximum directly on the chromatogram.
      
The flavor potentiating effects of four 5'-ribonucleotides and of sixteen amino acids were investigated with paired comparison tests and statistical evaluation of the results.
      
Fermentations ofStreptomyces coelicolor are known to convert lincosaminide antibiotics to mixtures of their inactive 3-(5'-ribonucleotides).
      
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Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter...

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter with hydrogen sulphide,followed by paper electrophoresis and paper chromatography.The purified product has been separated into four flavin-adenine peptides with different amino acid contents.One fraction with comparatively high mobility on paper electrophoresis and containing 12 amino acids(hydrolyzed in 6 N HCl) has an absorption spectrum with maxima at 265,350 and 450 mμ(compared with 260,375 and 450 mμ of FAD),the ratio of E_(260) and E_(450) mμ is equal to 3.87.The other three fractions has similar absorption spectra as that of the first,except for a slight shift of the 265 mμ maximum to 270 mμ.All the four flavin-adenine peptides contain cysteine and show a greenish yellow fluorescence in the u.v.light.The fluorescent intensity of the prosthetic group varies with pH and exhibits a maximum at pH 2.9.All fractions are inactive in the D-amino acid oxidase test and give on analysis 1 mole of adenine and 2.5 moles of phosphorus per mole of flavin.The pentose flavin ratio was much higher than that of FAD. Photolysis of the flavin-adenine peptides in alkaline solution yields a product which is insoluble in chloroform after acidification.Removal of the adenine results in the formation of flavin peptides.These facts indicate that the peptide chain is linked to the isoalloxazine nucleus of the prosthetic group.It is known that the absorption peak at 375 mμ of either FAD or FMN shifts to about 355 mμ at pH 12 due probably to the enolization of the keto group at the 2 or 4 position resulting in a redistribution of double bonds in the isoalloxazine ring system. In contrast our flavin-adenine peptide has the corresponding absorption maximum at 350 mμ which shows little positional shift at pH 12.This seems to suggest that the linking of the peptide chain to the isoalloxazine nucleus affects the enolization of the-NH-CO-,which may probably be the site of the linkage. The iron content increases with the specific activity of the enzyme during purification.Iron in the purified enzyme is present in the reduced state.It is firmly bound to the enzyme and can not be removed by prolonged dialysis against phosphate buffer or tris-hydroxymethyl-amino-methane buffer.Enzymatic activity is lost during prolonged incubation with o-phenanthroline or α,α'- dipyridyl and can not be recovered by incubation with Fe~(2+) or Fe~(3+).These experiments de- monstrate a close relationship between enzyme activity and the iron present in the enzyme molecule. The enzyme activity is lower in borate than in phosphate buffer.When 40 mM ethylenedi- aminetetraacetic acid is added to the borate buffer,the enzyme activity is raised almost to the level of that observed in the same concentration of phosphate buffer.The effect of EDTA and phosphate,when present together,is somewhat higher than of either alone.Alanine has a similar effect'as EDTA.

(一)用结晶胰蛋白酶及结晶胰凝乳蛋白酶处理净化的水溶性琥珀酸脱氢酶,经过对甲酚抽提,硫酸汞沉淀,硫化氢分解及纸电泳纸层析等方法净化得到四种带有不同肽链的腺嘌呤异咯嗪核苷酸。从它们的组成成份的分析以及它们的性质的观察,我们认为它们与已知的腺嘌呤异咯嗪二核苷酸略有不同。肽链是连接在异咯嗪上,其连接方式异于一般异咯嗪蛋白。肽链部份的氨基酸组成的分析结果,证明它们都含有半胱氨酸。(二)琥珀酸脱氢酶中的铁处于还原状态。铁与酶朊紧密结合,它与酶活力有密切关系。(三)无机磷可增加琥珀酸脱氢酶的活力,但琥珀酸脱氰酶的活力并不是必需依靠无机磷的存在,乙二胺四乙酸与丙氨酸也有类似无机磷的作用。

A method based upon the differences in ultraviolet absorption of the purine and pyrimidine bases at different pH is developed for the analysis of these components in purified ribonucleic acid. In a solution containing adenine, guanine, cytosine and uracil, optical densities are measured at 4 different wave lengths both at pH 1 and pH 13, and the amount of each base can be calculated by making use of the proper optical density differences. This method is simpler in procedure in comparison with other methods....

A method based upon the differences in ultraviolet absorption of the purine and pyrimidine bases at different pH is developed for the analysis of these components in purified ribonucleic acid. In a solution containing adenine, guanine, cytosine and uracil, optical densities are measured at 4 different wave lengths both at pH 1 and pH 13, and the amount of each base can be calculated by making use of the proper optical density differences. This method is simpler in procedure in comparison with other methods. Its reproducibility is satisfactory and the recovery falls within the range of 94-106%.

(一)核酸碱基在—定pH情况下产生(?)构并表現出不同的紫外綫吸收的特性。利用pH1和pH13的溶剂条件,在AGCU四种混合碱基体系中,由不同两处波长的光密度差值,可以求解出其中每种碱基的含量。本方法适用于合有AGCU四种碱基的提純核糖核酸样品,测定步驟簡单,重复性恆定,回收率在94~106%之間。(二)核酸样品經过氯酸降解后,降解液用蒸餾水稀释至相当于2.0N HClO_4的浓度。离心,吸取一定量的上清液,分別加入标准的HCl及NaOH溶液,調整酸碱度至相当于0.10N HCl(pH1)及0.10N NaOH(pH13)。酸液在249mμ,262mμ,274mμ和290mμ处,碱液在256mμ,258mμ,282mμ和290mμ处,分別用分光光度計测定光密度。测定溶液的适宜总浓度为60~160微克/5.0毫升。按下列公式計算混合体系中碱基的含量。U=0.194·△D(290_(13))-290_1·V A=0.108·△D_(262_1-282_(13))·V—0.221U G=0.198·△D_(249_1-258_(13))·V—0.542U C=0.127·△D_(274-256_(13))·V—0....

(一)核酸碱基在—定pH情况下产生(?)构并表現出不同的紫外綫吸收的特性。利用pH1和pH13的溶剂条件,在AGCU四种混合碱基体系中,由不同两处波长的光密度差值,可以求解出其中每种碱基的含量。本方法适用于合有AGCU四种碱基的提純核糖核酸样品,测定步驟簡单,重复性恆定,回收率在94~106%之間。(二)核酸样品經过氯酸降解后,降解液用蒸餾水稀释至相当于2.0N HClO_4的浓度。离心,吸取一定量的上清液,分別加入标准的HCl及NaOH溶液,調整酸碱度至相当于0.10N HCl(pH1)及0.10N NaOH(pH13)。酸液在249mμ,262mμ,274mμ和290mμ处,碱液在256mμ,258mμ,282mμ和290mμ处,分別用分光光度計测定光密度。测定溶液的适宜总浓度为60~160微克/5.0毫升。按下列公式計算混合体系中碱基的含量。U=0.194·△D(290_(13))-290_1·V A=0.108·△D_(262_1-282_(13))·V—0.221U G=0.198·△D_(249_1-258_(13))·V—0.542U C=0.127·△D_(274-256_(13))·V—0.133U AGCU为碱基的微克分子,V为測定溶液的体积毫升数。(三)核酸样品經1.0N HCl降解后,在嘌呤碱基和嘧啶核苷酸的混合体系內,可按以下公式求出胞嘧啶核苷酸或胞嘧啶含量。C=0.149·△D_(290_1-290_(13))·V C为碱基的微克分手数,V为测定溶液体积的毫升数。(四)在四种碱基不同时存在的体系中,本方法可用作为鉴定体系中的主要碱基种类,并可得到所合碱基的近似含量。

The oxidation of NADPH in the microsomal and supernatant fractions of the rat liver has been studied with the vibrating platinum electrode as well as the spectrophotometric method. An active system for the oxidation of NADPH by air, which was insensitive to cyanide, dicumarol, amytal, azide and 2,4-dinitrophenol, has been observed in the microsome fraction. It seems likely that the cytochrome oxidase system is not involved. The supernatant fraction of the liver homogenate obtained after centrifugation at 45,000×g...

The oxidation of NADPH in the microsomal and supernatant fractions of the rat liver has been studied with the vibrating platinum electrode as well as the spectrophotometric method. An active system for the oxidation of NADPH by air, which was insensitive to cyanide, dicumarol, amytal, azide and 2,4-dinitrophenol, has been observed in the microsome fraction. It seems likely that the cytochrome oxidase system is not involved. The supernatant fraction of the liver homogenate obtained after centrifugation at 45,000×g failed to catalyze the oxidation of NADPH by molecular oxygen. However, it contained a highly active reduced nicotinamide nucleotide dehydrogenase, which was found to be sensitive to dicumarol and was probably identical with the 'DT' diaphorase reported by Ernster et al. When such a supernatant fraction was added to the microsome preparation, a marked increase up to about 200% in the activity of the system for the oxidation of NADPH by air has been observed. At the same time about one half of the overall activity of this system became sensitive to dicumarol. It seems clear that the reduced nicotinamide nucleotide dehydrogenase in the supernatant fraction may be linked to certain components of the microsome fraction to produce a system capable of affecting the oxidation of NADPH by oxygen; such a system does not seem to have been hitherto reported. The physiological significance of this system for the oxidation of NADPH has been discussed.

利用自动記录白金微氧电极定氧与分光光度計测定的方法,发現大白鼠肝脏微粒体能氧化NADPH。微粒体的NADPH氧化系統活力不受氰化物、双香豆素、2,4二硝基酚、5乙,5(?)戊巴比妥酸鈉与迭氮酸鈉等所抑制。鼠肝匀浆經45,000×g离心所得的上清液不能催化NADPH被氧气所氧化。但含有对双香豆素高度敏感的还原菸酰胺核苷酸脫氫酶,其性貭与Ernster所提出的“DT黃递酶”很相似。微粒体制剂中加入上清液后NADPH氧化系統活力增大約1倍左右。此时若加入双香豆素,則NADPH氧化系統总活力被抑制大約一半。我們认为上清液中对双香豆素敏感的还原菸酰胺核苷酸脫氫酶能通过某种与微罫宓牧到M成NADPH氧化系統。这样NADPH就可以不需通过线粒体氧化。我們认为在鼠肝中NADPH的氧化途径如下: →还原性生物合成NADPH—?→通过綫粒体氧化(包括通过轉氫酶的作用) →微粒体NADPH氧化系統→O_2 →还原菸酰胺核苷酸脫氫酶→微粒体上未知系統(可能和微粒体上NADPH氧化系統部分相同) →O_2

 
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