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核苷酸    
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  “5'-核苷酸”译为未确定词的双语例句
    Study on Detection and Identification of Roundup Ready Soybean Using Oligonucleotide Microarray
    利用寡核苷酸芯片对Roundup Ready转基因大豆检测及鉴定技术的研究
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    STUDY OF CHARACTERISTICS OF IMMOBILIZED POLYNUCLEOTIDE PHOSPHORYLASE
    固定化多核苷酸磷酸化酶的特性研究
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    A Study on Separation Method of Nucleotide Preparing from Cane Molasses
    甘蔗糖蜜生产核苷酸的分离方法研究
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    Methods: By comparing the sequences of 16S rDNA and 23S rDNA gene of some pathogens in the GenBank, the four primers chosen were located in the conserved region and the oligonucleotide probes designed in the variable region.
    方法:将致病菌的16S rDNA和23S rDNA全序列进行软件比对,在可变区和恒定区分别设计特异性寡核苷酸探针和通用性引物,点样于玻片制成基因芯片。
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    5'-nucleotide of lentinus extract handled with 5'-phospho-diesterase was enhanced about 4.53%.
    由5’-磷酸二酯酶处理的香茹抽提物中的5’-核苷酸含量提高了4.53%。
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This paper deals with the process of digesting Na-DNA obtained from fish sperm into 5′-deoxymononucleotides by using 5′-phosphodiesterase from Penicillium citrinum, M71.The 201×8 anion exchanger resin was used in the seperation. The conditions for setting samples are as follows: Height of bed column 105 mm Diameter of bed column 45 mm Sample concentration 213 mg digest/ml Eluting flow-rate 0.5 ml/cm~2·min The results of the seperation indicated 5′-deoxymonoculeotides components could be fully eluted as a whole...

This paper deals with the process of digesting Na-DNA obtained from fish sperm into 5′-deoxymononucleotides by using 5′-phosphodiesterase from Penicillium citrinum, M71.The 201×8 anion exchanger resin was used in the seperation. The conditions for setting samples are as follows: Height of bed column 105 mm Diameter of bed column 45 mm Sample concentration 213 mg digest/ml Eluting flow-rate 0.5 ml/cm~2·min The results of the seperation indicated 5′-deoxymonoculeotides components could be fully eluted as a whole peak by the eluting agent,0.005 NHCl and 0.04 MNaCl. And the use of a few proper eluting agents,i.e.0.0018 NHCl,0.0028 NHCI, 0.036 M NaCl (ph 6.0) and 0.005 NHCl-0.02 M NaCl ccould bring about perfect and respective elution of dCMP,dAMP,dTMP and dGMP.

采用20■×8的离子交换层析柱(氯型,200~400目;柱高×柱截面为10.5cm×16cm~2)可将 Penicillium citrinnm变异菌株产生的核酸酶 P_1对鱼精 DNA 钠盐的酶解产物——四种脱氧单核苷酸完全分离。其分离工艺条件是:0.0018N HCl,0.0028N HCl,0.035N NaCl(pH6)和0.02M NaCl—0.005N HCl(dCMP→dAMP→dTMP→dGMP);酶解液浓度2.3mg/ml左右;加样流速0.1~0.2ml/cm~2·min;洗脱流速0.5ml 左右/cm~2·min。

Studying contents of inorganic phosphorus, free nucleotide, phospholipid, DNA, RNA, phosphoprotein and protein in ovaries of Haplotropis brunnerian Saus. and Liacala brevitarsis Lewis, the author connected the Schnider separating method with the Schmidt & Thannhauser methods thus improved the determing method. After degradation of RNA with NaOH, the HCl was used in replacement of PCA and TCA to force the NaCl produced (cone. 0.15 N). In this solution, the solubility of the DNA was negligible, so more favourable...

Studying contents of inorganic phosphorus, free nucleotide, phospholipid, DNA, RNA, phosphoprotein and protein in ovaries of Haplotropis brunnerian Saus. and Liacala brevitarsis Lewis, the author connected the Schnider separating method with the Schmidt & Thannhauser methods thus improved the determing method. After degradation of RNA with NaOH, the HCl was used in replacement of PCA and TCA to force the NaCl produced (cone. 0.15 N). In this solution, the solubility of the DNA was negligible, so more favourable to separate RNA and DNA. The contents of RNA determined by the modified method were compared with that by the phospho-fixing method and ultraviolet-absorption method, and the protein contents by Folin-phenal method and ultraviolet-absorption method. The results showed that the modified separating method is accurate, simple, less materials required and could separate seven components at the same time.

本文在研究昆虫雌虫卵巢内无机磷、游离核苷酸、磷脂、RNA、DNA、磷蛋白和蛋白质的含量时,将Schnider分离法、Schmidt和Thannhauser分离法结合,并进行改进。在用NaOH降解RNA后,改用HCl代替PCA或TCA,使之生成的NaCl(浓度为0.15N),在此溶液中DNA溶解度很小,更利于分离RNA与DNA。并以定磷法和紫外吸收法对比测定RNA含量,以Folin-phenal法与紫外吸收法对比测定蛋白质含量,表明改进的分离方法测定的结果准确,方法简便,取材少,可一次性分离上述7种成份。

The characteristics of Immobilized polynucleotide which is made up of polyaerylamidegel is compaed with that of the free enzyme. The result shows that when CDP is usud as basic material, the free enzyme 's km is equal to 6.4×10~(-3)m while the immobilized enzyme is equal to 9.14×10~(-3)m which is larger than that of enzyme. The proper temperature range of immobilized enzyme has been widened and its stabilized enzyme has been improved.

对用聚丙烯酰胺凝胶,制备的固定化多核苷酸磷酸化酶与游离酶的特性比较表明:在以CDP为底物时,游离酶的km=6.4×10~(-3)M,而固定化酶的km(表观)=9.14×10~(-3)M,比游离酶增大,固定化酶的最适温度范围扩宽,稳定性提高。

 
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