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细胞流动
相关语句
  cell flow
     Research on Immobilized Algae Cell Flow Bed for Treatment Living Sewage
     固定化藻类细胞流动床处理生活污水研究
短句来源
     Statistically calculation of bacterial number had been shown there was no significent difference between DNA fluorescence dye and cell flow counter(P<0.01).
     应用DNA荧光染料法测定的瘤胃细菌数与用细胞流动器测得的数值相比 ,从统计分析来看 ,没有显著差异(P<0.01)。
短句来源
  “细胞流动”译为未确定词的双语例句
     Establish Cultured Model of Endothelial Cells in a Flow Environment
     内皮细胞流动培养模型的建立
短句来源
     We studied the effect of shear stress (SS) on intercellular adhesion molecule 1 (ICAM 1) expression of rat brain microvascular endothelial cells (RBMECs) using the parallel plate flow chamber method.
     利用内皮细胞流动小室方法 ,对大鼠脑微血管内皮细胞在剪切力作用下细胞内粘附分子 1(ICAM 1,intercellularadhesionmolecule 1)的表达进行了研究。
短句来源
     The cell array system makes up of the cellular pipe, the vacuum chamber and the micro vacuum pump.
     设计了细胞排列的细胞流道、真空腔,选择了合适的细胞流动真空泵,实现了无重合信号细胞流动排队方法。
短句来源
  相似匹配句对
     Development of Flowing Cell Sensor
     流动细胞传感器的研制
短句来源
     Flow Cyto Fluorescence and Analysis of Cellular DNA Content
     流动细胞荧光和DNA含量分析
短句来源
     ALBUMINOUS CELLS
     蛋白细胞
短句来源
     Tom Clancys Splinter Cell
     细胞分裂
短句来源
     The Space of Flows
     流动空间
短句来源
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  cell flow
The initial disturbances, specified against the background of a steady cell, are coaxial with the cell flow and have various swirl directions, intensities, and dimensions.
      
If the disturbance intensity is small as compared with that of the convective-cell flow, the growth of the azimuthal velocity circulation in the perturbing vortices depends linearly on their initial circulation.
      
The difficulties involved by using a reference cell flow in gradient elution techniques where the eluent also absorbs are described briefly.
      
We also find G2-phase arrest and increase apoptosis by cell flow cytometry after treatment when ECT and/or radiation.
      
Bone marrow (BM) cell and/or peripheral blood mononuclear cell flow cytometric analyses of the following antigens were performed: HLA-DR, CD19, CD20, CD22, CD23, CD25, CD10, SmIg, kappa, lambda, CD79b, CD38, FMC7, CD3, CD2, and CD5.
      
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To determine the effects of fluid shear stress on microvascular endothelial cell monolayers, we designed a flow chamber to investigate the cytoskeleton changes after a period of shear stress effect. A coverslip carrying confluent rat brain microvascular endothelial cell monolayers was inserted into the device and the chamber gave parallel plate flow. The experiment proceeded with various perfusion rates. The perfusion medium was D-Hank's balanced salt solution with the viscosity of 0.75 mPa.s at 37℃. The shear...

To determine the effects of fluid shear stress on microvascular endothelial cell monolayers, we designed a flow chamber to investigate the cytoskeleton changes after a period of shear stress effect. A coverslip carrying confluent rat brain microvascular endothelial cell monolayers was inserted into the device and the chamber gave parallel plate flow. The experiment proceeded with various perfusion rates. The perfusion medium was D-Hank's balanced salt solution with the viscosity of 0.75 mPa.s at 37℃. The shear stress effected on the monolayers were 0.17, 0.35, 0.70 and 1.41 dyn/cm2 by a roller pump for a perfusion period of 30min, 1h and 3h respectively. The whole device was brought to 37℃ with an air curtain incubator. After a period of perfusion, the coverslip was taken out and then stained with Coomassie BB and FITC-Phalloidin. The stained coverslip was placed on the stage of an inverted tissue culture microscope with camera. The monolayer photos were analyzed by image processing and analysis system. On all the shear stress level cytoskeleton showed the similar changes. Cytoskelleton were rearranged parallel to the direction of shear stress.Immunofluorescence showed that the number of F-Actin was significantly increased after a period of 30min. The intercellular space increased and monolayers shank. The results showed the significant rearrangement of cytoskeleton caused by shear stress and transfer from G-Action to F-Actin which indicated that the brain microvascular endothelial cell monolayers might be more sensitive to the fluid shear stress.

利用自行研制的细胞流动小室对大鼠脑微血管内皮细胞在剪切力作用下细胞骨架蛋白的结构改变进行了初步研究,结果提示脑微血管内皮细胞在剪切力作用下,细胞形态学发生明显改变,细胞间隙增大、皱缩、脱落,细胞骨架蛋白的结构也有类似的变化,骨架蛋白沿流动方向重新排列,微丝中F-Actin的数量增加、变粗。这些改变的直接后果是内皮细胞通透性的增加。该工作为进一步开展剪切力对微血管内皮细胞功能、代谢等方面的影响提供了实验数据

To study the effects of fluid shear stress on microvascular endothelial cell monolayers, we designed a flow chamber to investigate the c-fos protein changes after a period of shear stress applied. A coverslip carrying confluent rat brain microvascular endothelial cell monolayers was inserted into the device and the chamber gave parallel plate flow. The experiment proceeded with various perfusion rates. The perfusion medium was M199 with 20% calf serum and the viscosity was 0.75 mPa.s at 37℃. The shear stress...

To study the effects of fluid shear stress on microvascular endothelial cell monolayers, we designed a flow chamber to investigate the c-fos protein changes after a period of shear stress applied. A coverslip carrying confluent rat brain microvascular endothelial cell monolayers was inserted into the device and the chamber gave parallel plate flow. The experiment proceeded with various perfusion rates. The perfusion medium was M199 with 20% calf serum and the viscosity was 0.75 mPa.s at 37℃. The shear stress effected on the monolayers were 0.14 and 0.28×10-5N/cm2 by a roller pump for a perfusion period of 1.5h, 2h and 3h. The whole device was brought to 37℃ with an air curtain incubator. After a period of perfusion, the coverslip was taken out and the c-fos protein was labeled by means of immunohistochemistry methods. After 0.14 and 0.28×10-5N/cm2 with 1.5h, 2h and 3h, MVEC were stained with c-fos immunohistochemistry kit. The cover slip was then processed by means of computer image analysis system to measure the density of MVEC monolayers. The c-fos protein was not observed in normal MVEC nucleus but was observed significantly in MVEC nucleus after shear stress applied. Those results suggested that fluid shear stress does lead to a cascade of downstream signaling events. The expression of c-fos protein showed the activation of ras-MEK-ERK1/2 pathway mediated by tyrosine kinase.

利用内皮细胞流动小室方法对大鼠脑微血管内皮细胞在剪切力作用下细胞核内原癌基因c -fos蛋白的翻译水平的表达进行了初步研究 ,结果提示脑微血管内皮细胞在剪切力作用下 ,c -fos蛋白有明显的表达且显示了剪切力水平的非依赖性和作用时间的依赖性。流动剪切力诱导的c -fos的适度表达可以导致内皮细胞伸展、细胞骨架蛋白合成、细胞骨架重排等 ,以适应流动剪切力的作用。但过度的剪切力作用可以引起内皮细胞皱缩、损伤、脱落 ,这时的c-fos会有过度的表达。如何控制c -fos蛋白的表达量 ,可能是阻断内皮细胞损伤的可能途径之一。该工作为进一步开展剪切力对微血管内皮细胞信号转导机制的影响提供了实验数据。

A primary study of shear stress activated K+ channel in Microvascular Endothelial Cell,MVEC using patch-clamp technique and parallel-plate flow chamber was reported in this papaer. MVEC monolayers were voltage-clamped and whole-cell currents were recorded. Cells were voltage clamped at -100mv and step depolarized to various potentials for 2100ms at 0.1Hz. Shear stress was applied with a pressure ejection micro-tube placed near the MVEC. The flow rate was 100μl/s and the diameter of that tube was 10~15μm....

A primary study of shear stress activated K+ channel in Microvascular Endothelial Cell,MVEC using patch-clamp technique and parallel-plate flow chamber was reported in this papaer. MVEC monolayers were voltage-clamped and whole-cell currents were recorded. Cells were voltage clamped at -100mv and step depolarized to various potentials for 2100ms at 0.1Hz. Shear stress was applied with a pressure ejection micro-tube placed near the MVEC. The flow rate was 100μl/s and the diameter of that tube was 10~15μm. The shear stress were about 0.05~0.12N/m2. Whole-cell currents were observed and the currents were inhibited in a concentration-dependence manner by bath application of TEA-Cl with an IC50 approximately 2.0mmol/L. After application of shear stress, currents increased in amplitude as compared with controls. The absolute currents activated by shear stress and I-V curve with shear stress application were obtained. When MVEC membrane potential was 30mV, the potassium channel current was 54.12±24.42pA, 106.13±47.42pA at 60mV, 154.01±53.22pA at 90mV (n=3,p<0.01). These results supported that shear stress sensitive ion channel was really existed on the membrane of microvascular endothelial cell.

利用膜片钳及内皮细胞流动小室方法对大鼠脑微血管内皮细胞在剪切力作用下内皮细胞膜K+ 通道的开放进行了初步研究 ,结果提示脑微血管内皮细胞膜上存在剪切力敏感的K+ 通道 ,剪切力作用后 ,内皮细胞膜上K +电流明显增大 ,此电流有明显的短暂延迟现象 ,也可以被胞外施加的TEA抑制 ,符合IKv特征。流动剪切力可以通过影响内皮细胞膜上的K +通道的开放引起穿细胞的离子通透性的增加 ,进而引起细胞内Ca2 + 的变化。在K+ 、Ca2 + 等离子浓度改变的诱导下可以促使G -Actin装配为F -Actin。同时诱导内皮细胞内钙库调节机制的激活 ,这些变化都可以进一步引起细胞信号转导机制的激活。该工作为进一步开展剪切力对微血管内皮细胞信号转导机制的影响提供了实验数据。

 
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