助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   lectin基因 的翻译结果: 查询用时:0.545秒
图标索引 在分类学科中查询
所有学科
轻工业手工业
更多类别查询

图标索引 历史查询
 

lectin基因
相关语句
  lectin gene
     The nested PCR and semi-nested PCR tests were used to detect the transgenic ingredient in Roundup Ready soybean oil,the results showed that Lectin gene(112bp),CaMV35S gene(147 bp) and CP4-EPSPS gene(205 bp)could be amplified from crude soybean oil but not from finished product of soybean oil.
     利用nested PCR和semi-nested PCR检测大豆(Roundup Ready)油中的转基因成分发现,该方法能够检测到大豆原油中的Lectin基因(112bp)、CaMV35S基因(147bP)和CP4-EPSPS基因(205bp),检测灵敏度达到10-6ng/μl;
短句来源
     The nucleotide acid sequences of the four fragments had more than 95% homology with Lectin gene (AF001527) and Pectate Lyase gene (x92943) which were submitted to GenBank .
     此四条片段的核苷酸序列分别与GenBank中发表的Lectin基因(AF001527)及Pectate Lyase基因(X92943)具有95%以上的同源性。
短句来源
     At the same time,the Amino Acid sequences that were induced from the cDNA and DNA fragments of Lectin gene had more than 93% homology with the Amino Acid sequence of Lectin gene (AF001527 ).
     同时,香蕉凝集素(Lectin)基因的cDNA片段和DNA片段所推测出的氨基酸序列与GenBank中发表的Lectin基因(AF001527)的氨基酸序列也具有93%以上的同源性。
短句来源
     tumefaciens strain CP4. The triplex PCR of inner LECTIN gene, CaMV 35S promoter and NOS terminator, duplex PCR of inner LECTIN and EPSPS gene were carried on successfully, and obtained the same results as expected.
     最后成功进行了扩增内源LECTIN基因、CaMV35S启动子和NOS终止子的三重PCR分析,以及内源LECTIN基因和EPSPS基因的二重PCR分析,得到预期结果.
短句来源
     After milling, cooking and homogenizing, the lectin gene fragment is degraded to <1000 bp.
     磨浆、煮浆、均质等工艺,使lectin基因降解至1000bp以下;
短句来源
更多       
  “lectin基因”译为未确定词的双语例句
     Cloning and Expression of Lectin of Human P-selectin
     人P选择素lectin基因片段的克隆及表达
短句来源
     Conclusion: Single nucleotide polymorphism at position 98 in the second exon of E-selectin gene exited in Han population of Hubei, and its distribution was significantly different among ethnics.
     结论 :在湖北地区汉族人群中存在E se lectin基因第 2外显子 98位点的单核苷酸多态性 ,这种多态性在种族间可能存在着较大的差异
短句来源
     According to the plasmid map of genetically modified soybean(Roundup Ready), three exogenous gene( CaMV35S promoter, NOS terminator, NOS/EPSPE gene) and one endogenous gene ( Lectin ) were selected as target genes to design and synthesize their primers. The probe was synthesized using the method of nick translation and labeled with DIG dUTP.
     为提高对转基因大豆的监督检测能力 ,研制了转基因大豆DNA检测芯片。 根据转基因大豆 (RoundupReady)中所转入的外源基因 ,选择CaMV35S启动子、NOS终止子、NOS EPSPE基因和内源Lectin基因设计特异性引物 ,采用多重PCR法对待测样品进行扩增 ,通过缺口平移法合成DIG dUTP标记杂交探针 ,并制备基因芯片。
短句来源
     PCR amplification of sequences of 35S, Nos, EPSPs and an endogenous reference gene lectin in 11 market available brand of refined soybean oil was performed. Results showed that the sequence-specific fragments were amplified in all of the samples, demonstrating that the method could be applied for detection of transgene sequences in refined soybean oil.
     提取市场上出售的十几种精练大豆油的DNA,针对35S、Nos、EPSPS和Lectin基因进行了PCR检测,分别扩增出不同的目的片段。
短句来源
  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Cloning and Expression of Lectin of Human P-selectin
     人P选择素lectin基因片段的克隆及表达
短句来源
     The gene was highly expressed in a soluble form in E.
     该基因在大肠杆菌E.
短句来源
     Gene Cloning and Expression of Human P-Selectin′s Lectin-EGF Domain
     人P选择素胞外区Lectin-EGF片段的基因克隆及表达研究
短句来源
查询“lectin基因”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  lectin gene
trichocephalum does not fall into the group of the tribe Trifolieae members according to both the amino acid sequence of lectin carbohydrate-binding region and the nucleotide sequence of lectin gene.
      
The data from Southern blot analysis indicated the presence of only one lectin gene in all Lens taxa except L.
      
Transgenic rice plants expressing the snowdrop lectin gene (gna) exhibit high-level resistance to the whitebacked planthopper (S
      
All taxa possessed at least one member of the true lectin gene.
      
Data from Southern blot analysis indicated the presence of only one lectin gene in P.
      
更多          


Objective[WT5”BZ] To investigate the adhesion molecules gene expression in murine sepsis induced liver injury.[WT5”HZ] Methods[WT5”BZ] Sepsis was induced in mice by cecal ligation and puncture(CLP). Sham operation group was used as control. The gene expressions of adhesion molecules (E selectin, ICAM 1 and VCAM 1) in liver sinusoidal endothelial cells were assessed by RT PCR. Changes of MPO and microcirculatory permeability in the liver were measured.[WT5”HZ] Results[WT5”BZ] A significant increase...

Objective[WT5”BZ] To investigate the adhesion molecules gene expression in murine sepsis induced liver injury.[WT5”HZ] Methods[WT5”BZ] Sepsis was induced in mice by cecal ligation and puncture(CLP). Sham operation group was used as control. The gene expressions of adhesion molecules (E selectin, ICAM 1 and VCAM 1) in liver sinusoidal endothelial cells were assessed by RT PCR. Changes of MPO and microcirculatory permeability in the liver were measured.[WT5”HZ] Results[WT5”BZ] A significant increase in E selectin gene expression was observed at 3 hours after CLP 0 85±0 06 vs. 0 22±0 04 ( P <0 01). And the gene expressions of ICAM 1 and VCAM 1 significantly increased between 3 and 12 hours after CLP 1 02±0 10 vs. 0 30±0 04( P <0 01),and 1 04±0 14 vs. 0 37±0 04( P <0 01). A significant increase in hepatic tissue MPO was also found between 3 and 12 hours after CLP 0 589±0 116 vs. 0 160±0 025 ( P <0 01).The microcirculatory permeability in the liver began to increase at 3 hours after CLP, and this increase became significant at 12 hours after CLP 0 049±0 007 vs. 0 034±0 004 ( P <0 01).[WT5”HZ] Conclusion[WT5”BZ] The up regulation of the gene expressions of adhesion molecules in liver sinusoidal endothelial cells may lead to the recruitment of leukocytes at the site of inflammation causing liver injury during sepsis.[WT5”HZ]

目的 探讨脓毒症小鼠肝损伤时肝窦内皮细胞粘附分子基因表达变化。方法 用盲肠结扎穿孔 (CLP)法制造小鼠脓毒症模型 ,假手术组 (sham)接受同样的手术操作但不行CLP。分别在致伤后 3、12h分离肝窦内皮细胞 ,用RT PCR的方法定量检测E selectin、ICAM 1和VCAM 1的基因表达 ,同时还测定了肝组织中MPO活性及微血管通透性的改变。结果 CLP后 3h肝窦内皮细胞E se lectin基因的表达明显增高 (0 85± 0 0 6vs.0 2 2± 0 0 4,P <0 0 1) ;ICAM 1基因的表达在CLP后 3h即明显增高 12h时达高峰 (1 0 2± 0 10vs.0 30± 0 0 4,P <0 0 1) ;VCAM 1基因的表达在CLP后 3h即明显增高 (1 0 4± 0 14vs.0 37± 0 0 4,P <0 0 1) ,至CLP后持续到 12h后肝组织中MPO活性在CLP后 3h明显增高 (0 5 89± 0 116vs.0 16 0± 0 0 2 5 ,P <0 0 1) ,持续到 12h。CLP后 3h肝微血管...

目的 探讨脓毒症小鼠肝损伤时肝窦内皮细胞粘附分子基因表达变化。方法 用盲肠结扎穿孔 (CLP)法制造小鼠脓毒症模型 ,假手术组 (sham)接受同样的手术操作但不行CLP。分别在致伤后 3、12h分离肝窦内皮细胞 ,用RT PCR的方法定量检测E selectin、ICAM 1和VCAM 1的基因表达 ,同时还测定了肝组织中MPO活性及微血管通透性的改变。结果 CLP后 3h肝窦内皮细胞E se lectin基因的表达明显增高 (0 85± 0 0 6vs.0 2 2± 0 0 4,P <0 0 1) ;ICAM 1基因的表达在CLP后 3h即明显增高 12h时达高峰 (1 0 2± 0 10vs.0 30± 0 0 4,P <0 0 1) ;VCAM 1基因的表达在CLP后 3h即明显增高 (1 0 4± 0 14vs.0 37± 0 0 4,P <0 0 1) ,至CLP后持续到 12h后肝组织中MPO活性在CLP后 3h明显增高 (0 5 89± 0 116vs.0 16 0± 0 0 2 5 ,P <0 0 1) ,持续到 12h。CLP后 3h肝微血管通透性即开始升高 ,至 12h时与sham组比较差异有显著意义 (0 0 49± 0 0 0 7vs .0 0 34± 0 0 0 4,P <0 0 1)。结论 脓毒症小鼠肝窦内皮细胞粘附分子表达增高可能是导致白细胞在损伤组织局部聚集进而造成器官功能损伤的重要因素。

PCR was applied to amplify lectin gene of P-selectin and cloned into pET42b(+). The sequenced vector was expressed in E.coli with high level fusion protein tailed with six additional histidine residues at its C-terminus,and the histidine-tagged lectin was purified with Ni 2+ -NTAsuperflow affinity chromatography, It's purifity reached 90%, 100 ml induction culture had 0.9 mg lectin-(His) 6 fusion proteins, reaching 15% of the total bacteria protein. SDS-PAGE and Western blot showed that the molecular...

PCR was applied to amplify lectin gene of P-selectin and cloned into pET42b(+). The sequenced vector was expressed in E.coli with high level fusion protein tailed with six additional histidine residues at its C-terminus,and the histidine-tagged lectin was purified with Ni 2+ -NTAsuperflow affinity chromatography, It's purifity reached 90%, 100 ml induction culture had 0.9 mg lectin-(His) 6 fusion proteins, reaching 15% of the total bacteria protein. SDS-PAGE and Western blot showed that the molecular weight of expressed lectin was 13 000.

应用PCR技术扩增P选择素lectin基因 ,克隆入pET42b (+)载体 ,测序验证后在大肠杆菌BL2 1中表达了lectinC端融合 6×His蛋白 ,表达产物经Ni2 + NTAsuperflow亲和柱纯化 ,纯度可达 90 %以上 ,得率为 0 9mg/ 10 0ml,其表达量达到总菌体蛋白的 15 %。SDS PAGE和Western印迹试验显示 ,表达产物相对分子质量为 130 0 0。

TheDNAwasextractedfromseveralconcentratedfeeds.Basedon theheterogenousgenesusedintransgeniccrops,thePCRwas performedwithprimersderivedfromCaMV35Spromoter,NOSterminator,EPSPSgene,andBt CryIA(b) gene,respectively.Endogenouscorn(Zea mays)zeingeneandsoybean(Glycine max)lectingenewereemployedas PCRinternalcontrol.Themethodestablishedherehasbeenemployedsucceessfullyindetectingtransgenicelementsinimportedfeedrawmaterials.

成功地从成品饲料中提取DNA,并采用PCR检测技术从中检出35S启动子、NOS终止子、EPSPS耐除草剂基因和CryIA(b)抗虫基因等转基因成分,同时通过扩增玉米(Zeamays)自身zein蛋白基因及大豆(Glycinemax)自身lectin基因的引物和阴阳性对照、阴阳性质控,避免产生假阳性、假阴性结果。该方法已在口岸进口饲料转基因检测中得到初步应用。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关lectin基因的内容
在知识搜索中查有关lectin基因的内容
在数字搜索中查有关lectin基因的内容
在概念知识元中查有关lectin基因的内容
在学术趋势中查有关lectin基因的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社