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southern杂交结果
相关语句
  southern blotting hybridization
     Southern blotting hybridization with genomic DNA showed that RIM9b is preset in single copy.
     Southern杂交结果表明该基因以单拷贝存在于水稻基因组中。
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  “southern杂交结果”译为未确定词的双语例句
     RT-PCR/Southern results shew that more abnormal CD44 mRNA transcripts were observed in the non-small cell lung carcinomas (NSCLCs) with lymph nodes metastasis than in non-small cell lung carcinomas without lymph node metastasis(X2 = 12. 13, P < 0. 01 ).
     RT-PCR/Southern杂交结果显示,有淋巴结转移较无淋巴结转移的非小细胞肺癌表达更多的 CD44 mRNA异常转录子(X2=12.13, P<0.01)。
短句来源
     Southern blot of EcoRⅠ cut DNA of B8,B8B,B8F with Tn5 fragment as probe showed that there was a band about 20 kb or 9 kb in size in B8B and B8F respectively,but no band in B8.Genomic DNA of two mutated strains were isolated respectively,cut with BamHⅠ,ligated to BamHⅠ cut vector pBS.
     用Tn5片段为探针,分别对B8、B8B、B8F进行Southern杂交,结果在B8中无特异条带,而在B8B和B8F中各有一条约20 kb和9 kb的EcoRⅠ特异条带;
短句来源
     by Southern blot transfer of DNA digested with BamH I, 4 c-fos oncogene bands of 5.4, 3.4, 1.8, 1.0kb and 3 c-myc oncogene bands of 7.4, 4.3, 3.4kb were observed, but there was no difference in these 4 stages.
     Southern杂交结果:CWE小鼠发育的各期均显示:5.4kb、3.4kb、1.8kb、1.0kb四条c-fos基因区带; 7.4kb、4.5kb、3.4kb三条c-myc基因区带。
短句来源
     Southern blotting result confirmed that of RT PCR assay.
     Southern杂交结果和RT PCR电泳结果完全一致 ;
短句来源
     By using the b2 fragment of Quox-1 gene as probe, we have confirmed that the Quox-1 gene homologous sequence exists in the human genome according to the results of Southern blot.
     以Quox-1基因的特异性片段b_2为探针,与人基因组DNA作Southern杂交,结果显示,人基因组中存在Quox-1基因的同源序列。
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     Fromthe Southern hybridization analysis seemsto suggest that chromosome DNA of C.
     Southern 杂交试验的结果显示出C.
短句来源
     It showed the similar result as that bySouthernblotting analysis.
     Southern杂交检测结果基本一致。
短句来源
     3 Southern blotting.
     3.Southern杂交
短句来源
     The result of Southern hybridization demonstrated that the differential fragments were true.
     Southern杂交结果证明了差异片段的真实性。
短句来源
     Multiplex real-time PCR was compared with Southern blot analysis in order to verify the method.
     与Southern杂交检测结果进行比较。
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  southern blotting hybridization
The relative expression of each Cart1 isoform was analysed by RT-PCR and subsequent Southern blotting hybridization.
      


A clone homologous to Zein gene has been isolated by using maize Zein gene as probe,and named RAS1. Physical map of the inserted rice DNA fragment (14.2kb) has been analyzed with restriction enzymes and Southern hybridization, A 0.9kb EcoR Ⅰ-Hind Ⅲ fragment among them has been identified to be the homologous sequence.

本研究以玉米醇溶蛋白基因(Zein)为探针,从水稻(IR36)基因文库中筛选出一个具有同源顺序的克隆λRASI,并制作了其插入水稻片断(14.2kb)的限制性内切酶物理图谱.Southern杂交结果表明,与Zein基因同源的区域位于λRASI插入序列的0.9kb EcoRI-HindⅢ片断上.

A simple method for the preparation of chloroplast DNA (ctDNA) from rice has been developed. The rice ctDNA prepared with this method has successfully been used in restriction enzyme mapping anl Southern transfer. The gene probe of PSIlP680ChlaAP from spinach was hybridized with Pst-1, Pst-14, Pvu-2 and Sal-1 fragments of rice ctDNA in Southern hybridization. According to the physical map of rice ctDNA drawn by Hirai and Zhao Yan, this gene located at the site nearby the RuBPCase LS gene and about 26 kb from...

A simple method for the preparation of chloroplast DNA (ctDNA) from rice has been developed. The rice ctDNA prepared with this method has successfully been used in restriction enzyme mapping anl Southern transfer. The gene probe of PSIlP680ChlaAP from spinach was hybridized with Pst-1, Pst-14, Pvu-2 and Sal-1 fragments of rice ctDNA in Southern hybridization. According to the physical map of rice ctDNA drawn by Hirai and Zhao Yan, this gene located at the site nearby the RuBPCase LS gene and about 26 kb from the nearer one of the inverted repeat region in the chloroplast genome of rice. The arrangement pattern oi these genes was observed in chloroplast genome in higher plants for the first time.

本实验总结出一套水稻叶绿体DNA的提取方法,并获得清晰的叶绿体DNA限制性内切酶图谱。Southern杂交结果表明,菠菜PSIIP680ChlaAP基因探针与水稻叶绿体DNA的Pst-1,Pst-14,Pvu-2和Sal-1片段的部分顺序有较高的同源性。根据Hirai和赵衍的水稻叶绿体基因组物理图,可以确定该基因位于紧靠RuBPCaseLS基因,距反向重复区约26kb处。高等植物叶绿体基因组中这种基因排列方式还未见报道。

The expression of 5 oncogenes was examined in 4 developmental stages (embryo, newborn, sexual maturity and adult)of CWE mouse brain tissue.Brain RNA was detected with Ha-ras, c-fos, c-myc v-erb B and mos oncogene probes by dot blot hybridization. The expression of these oncogenes was demonstrated.Ha-ras, c fos, c-myc were expressed at high level in the embryo and newborn brain, but erbB showed higher expression at the stage of sexual maturity. In contrast, mos oncogene showed no expression at all.At the same...

The expression of 5 oncogenes was examined in 4 developmental stages (embryo, newborn, sexual maturity and adult)of CWE mouse brain tissue.Brain RNA was detected with Ha-ras, c-fos, c-myc v-erb B and mos oncogene probes by dot blot hybridization. The expression of these oncogenes was demonstrated.Ha-ras, c fos, c-myc were expressed at high level in the embryo and newborn brain, but erbB showed higher expression at the stage of sexual maturity. In contrast, mos oncogene showed no expression at all.At the same time the DNA of brain tissue was studied by dotblot hybridization, the result showed that there was no oncogene amplification in any developmental stage; by Southern blot transfer of DNA digested with BamH I, 4 c-fos oncogene bands of 5.4, 3.4, 1.8, 1.0kb and 3 c-myc oncogene bands of 7.4, 4.3, 3.4kb were observed, but there was no difference in these 4 stages.

本文以Ha-ras.c-fos、c-myc、v-erbB、mos五种癌基因片段为分子探针,应用斑点杂交技术,对CWE纯系小鼠脑组织发育的四个时期(胚胎期、新生期、性成熟期、体成熟期)的RNA进行分子杂交分析。发现:在实验各期中这几种癌基因的表达有较明显的变化。Ha-ras、c-fos、c-myc基因的表达在胚胎期和新生期脑组织中较高;v-erbB基因的表达在性成熟期脑组织中较高;mos基因在实验各期均无表达。DNA斑点杂交结果:小鼠脑组织发育各期均未观察到癌基因扩增现象。Southern杂交结果:CWE小鼠发育的各期均显示:5.4kb、3.4kb、1.8kb、1.0kb四条c-fos基因区带;7.4kb、4.5kb、3.4kb三条c-myc基因区带。结果表明:不同细胞癌基因在小鼠胚胎发育和胚后发育中有不同的表达。

 
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