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   构建骨组织 的翻译结果: 查询用时:0.487秒
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构建骨组织
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  tissue-engineered bone
     Placenta DerivedStem Cells Produced Osteoid in Vitro and in Vivo, and the Constructive Effect of Tissue-engineered Bone Cultured in Bioreactor
     胎盘基质干细胞骨向分化及生物反应器构建骨组织的修复效果
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  “构建骨组织”译为未确定词的双语例句
     Conclusion Alginate of 1% is suitable for constructing carrier of tissue engineering bone.
     结论  1%海藻酸盐适宜与异种骨复合构建骨组织工程载体。
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     PHB possesses good biocompatibility and can be ideal scaffold material for bone tissue engineering.
     PHB泡沫材料有良好的生物相容性可以作为骨组织工程支架; MSC/PHB复合物可以用来构建骨组织和修复骨缺损
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     Experiment of allogenic deproteinized bone combined fibrin glue constructing bonetissue engineering scaffold in vitro
     脱蛋白骨复合纤维蛋白胶构建骨组织工程支架的体外实验研究
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     Experimental Study of the Bone Reconstruction Using BMSCs-Alginate Complex
     骨髓基质干细胞-藻酸钙复合物构建骨组织的实验研究
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     Conclusion Biomimetic mineralized can improve early stage cell adhesion rate of PLGA and is a good scaffold material for the bone tissue engineering.
     结论仿生矿化可以改善PLGA早期细胞黏附性能,是构建骨组织工程支架材料的较好方法。
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  相似匹配句对
     Tissue Engineered Fabrication of Bone and Cartilage Compsite
     -软复合组织构建的研究
     Tissue engineering of bone and cartilage composite
     -软复合组织构建的研究
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     CONSTRUCTION OF HUMAN OSTEOPROTEGERIN MAMMALIA VECTOR
     人保护素哺乳类表达载体的构建
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     Tissue-engineering bone fabricated with biphasic seeding in vitro
     双相接种法体外构建组织工程
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     Construction of hepatocyte-specific vectors
     基因载体的构建
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  tissue-engineered bone
The SIS can be used as a scaffold for constructing tissue-engineered bone as it can improve the formation of bone and vessels in vivo.
      
The tissue-engineered bone, which was constructed by combining zinc-sintered bovine cancellous bone with MSCs, has satisfactory osteogenic capabilities in vivo.
      
Repairing of goat Tibial Bone Defects with BMP-2 Gene-Modified Tissue-Engineered Bone
      
We combined tissue-engineered bone and gene therapy to provide osteoprogenitor cells, osteoinductive factors, and osteoconductive carrier for ideal bone regeneration in critical-sized bone defects.
      
It is thought that this kind of biodegradable glass scaffold combined with osteogenic cells has potential to be studied further as a tissue-engineered bone substitute.
      
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This paper addresses the question whether type Ⅰ col lagen receptor system is necessary for the functional activity of osteoblasts. Through blocking type Ⅰ collagen receptor system by type Ⅰ collagen antibody or integrin α2β1 antibody, the proliferation of osteoblasts was studied by cell count, the apoptosis was studied by flow cytometry, and the mRNA expressio ns of type Ⅰ collagen, integrin α2β1 and osteocalcin were also detected b y RT PCR techniques. When type Ⅰ collagen receptor system was blocked,...

This paper addresses the question whether type Ⅰ col lagen receptor system is necessary for the functional activity of osteoblasts. Through blocking type Ⅰ collagen receptor system by type Ⅰ collagen antibody or integrin α2β1 antibody, the proliferation of osteoblasts was studied by cell count, the apoptosis was studied by flow cytometry, and the mRNA expressio ns of type Ⅰ collagen, integrin α2β1 and osteocalcin were also detected b y RT PCR techniques. When type Ⅰ collagen receptor system was blocked, osteobl asts showed high apoptosis rate, lower growth kinetics and weak mRNA expression of type Ⅰ collagen, integrin α2β1 and osteocalcin; the blocking effect wa s reversible. It suggests that type Ⅰ collagen receptor system is necessary for osteoblastic phenotype. The biomaterials for bone tissue engineering should be constructed according to the extracellular matrix of osteoblasts, which supply n ormal extracellular environment for osteoblasts.

为进一步探讨I型胶原及其受体系统是否为成骨细胞功能活动所必需, 使用Ⅰ型胶原和整合素α2β1的一抗阻断Ⅰ型胶原整合素α2β1系统,以细胞计数法比较成骨细胞的增殖能力,流式细胞仪分析成骨细胞的凋亡情况,RTPCR技术研究I型胶原、整合素α2β1 及骨钙素的mRNA表达情况。结果发现阻断I型胶原整合素α2β1系统后,成骨细胞的增殖能力减弱,凋亡率增高,Ⅰ型胶原、整合素α2β1 及骨钙素的mRNA表达减少,其中I型胶原一抗的阻断作用大于整合素α2β1的一抗,这种阻断作用具有可复性,随抗体的去除可部分恢复,表明Ⅰ型胶原整合素α2β1系统为成骨细胞功能活动所必需,构建骨组织工程的支架材料时要考虑到骨组织的基质成份,应为成骨细胞提供相对正常的胞外基质环境。

?Objective:To investigate the behavior of absorbable coral hydroxyapatite (Interpore 500R) as bone tissue engineering scaffold. Methods: Marrow stromal stem cells harvested from legally aborted human fetus were cultured in DMEM containing 100 ml/L fetal boving serum and induced to differentiate towards osteoblasts.Then the cells were seeded onto absorbable coral hydroxyapatite , then the composite was implanted subcultaneously into the back of nude mice .Cell materia attachement was observed with scanning...

?Objective:To investigate the behavior of absorbable coral hydroxyapatite (Interpore 500R) as bone tissue engineering scaffold. Methods: Marrow stromal stem cells harvested from legally aborted human fetus were cultured in DMEM containing 100 ml/L fetal boving serum and induced to differentiate towards osteoblasts.Then the cells were seeded onto absorbable coral hydroxyapatite , then the composite was implanted subcultaneously into the back of nude mice .Cell materia attachement was observed with scanning electron microscope,type I collegen secretion was studied with laser confocal microscope and new bone formation was studied histologicaley. Results: The cells could adhere to absorbable coral hydroxyapatite and secrete collagen I.Mature bone tissue was found in the implanted composite 40 day after transplantation. Conclusion: Absorbable coral hydroxyapatite Interpore 500R is a favorable bone tissue engineering scaffold.

目的 :研究可吸收性珊瑚羟基磷灰石 (Interpore 5 0 0R)作为骨组织工程支架材料的特点。 方法 :取人骨髓基质干细胞于含 10 0ml/L胎牛血清的DMEM培养液中培养 ,并向成骨细胞诱导。将细胞与可吸收性珊瑚羟基磷灰石复合 ,通过扫描电镜观察细胞附着情况 ,共聚焦显微镜观察I型胶原的分泌。同时将细胞 /Interpore5 0 0R复合物植入裸鼠体内构建骨组织 .40d后取裸鼠标本组织切片、HE染色观察成骨情况。结果 :骨髓基质干细胞在可吸收性羟基磷灰石表面附着良好 ,可分泌I型胶原 ,在裸鼠体内形成组织工程骨。结论 :可吸收性羟基磷灰石生物相容性好 ,细胞附着后仍继续保持成骨细胞特性 ,并可形成骨组织。作为骨组织工程的支架材料 ,应用前景良好。

Objective To investigate the ability of heterotopic osteogenesis and reconstruction of critical size calvarial defect, a bone tissue engineering Sprague Dawley rat model with autogenous osteoblasts loading on porous calcium phosphate ceramic was established. Methods Bone marrow was obtained from femurs and tibias of 20 male adult SD rats under general anesthesia and sterile condition. Autogenous osteoblasts proliferated and differentiated in α minimal essential medium with some special additives for 14 days....

Objective To investigate the ability of heterotopic osteogenesis and reconstruction of critical size calvarial defect, a bone tissue engineering Sprague Dawley rat model with autogenous osteoblasts loading on porous calcium phosphate ceramic was established. Methods Bone marrow was obtained from femurs and tibias of 20 male adult SD rats under general anesthesia and sterile condition. Autogenous osteoblasts proliferated and differentiated in α minimal essential medium with some special additives for 14 days. Culture cells were then seeded and subcultured for another 15 days in porous calcium phosphate bioceramic, which had been soaked in fibronectin for surface modification. The cell ceramic compound was implanted subcutaneously and intramuscularly as well as used to repair critical sized (8 mm diameter) calvarial defect in the same rat. Lab animals were sacrificed at two week intervals till 20th week postoperatively and all the samples were dissected.Results Morphological and histological study demonstrated that cell ceramic compound had the ability of heterotopic osteogenesis. It was also observed that reconstruction of critical size calvarial defect with this hybrid material was better than that with solitary ceramic.Conclusion It seems that the experimental results got from this closed colony animal model were more similar to clinical situation. This model is superior to other homogenic or immune deficiency ones.

目的 构建封闭群动物SD大鼠骨组织工程模型 ,研究含孔磷酸钙陶瓷为支架复合自体成骨细胞的异位骨形成和极量骨缺损修复能力。方法 成年雄性SD大鼠 2 0只 ,全麻及无菌条件下取其左侧股骨及胫骨 ,将骨髓冲入 75ml培养瓶中并在诱导培养条件下进行体外扩增分化 ;培养 1 4d后将已具成骨细胞表型的细胞消化接种于经纤连蛋白表面修饰的含孔磷酸钙陶瓷上继续培养 1 5d ,再对应地将细胞 陶瓷复合体种植于大鼠皮下、肌肉内并用于颅骨极量缺损修复。分别于 2~ 2 0周处死动物 ,从形态学及组织学角度观察比较异位骨形成及骨缺损修复情况。结果 细胞 陶瓷复合体无论种植于皮下或肌肉内均表现明显的异位骨形成能力 ;颅骨极量缺损修复也优于同期仅植入经表面修饰但无细胞的陶瓷对照组。结论 应用封闭群动物SD大鼠构建的骨组织工程模型避免了采用免疫缺陷动物及同源近交系动物价格昂贵、近交衰退等缺点 ,其结果接近于临床 ,因而更具有说服力

 
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