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日本梨树
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  japanese pear
     Cloning and Nucleotide Sequence of the cDNA Encoding a β-1,3-Glucanase-like Protein Secreted from Growing Pollen Tubes of Japanese Pear (Pyrus pyrifolia)
     编码日本梨树(Pyruspyrifolia )花粉类β-1,3-葡聚糖酶蛋白的cDNA克隆与核苷酸序列(英文)
短句来源
     A pollen cDNA library of Japanese pear (Pyrus pyrifolia ) of the family Rosaceae was constructed and a cDNA (bgn-1) of 1 408 bp, which encodes a protein (BGN-1) secreted from growing pollen tubes, has been cloned and sequenced.
     构建了日本梨树(Pyruspyrifolia)花粉cDNA文库, 发现了一个编码花粉管分泌的类β-1,3-葡聚糖酶蛋白(BGN-1)的cDNA(bgn-1 )并测定了其序列。
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  相似匹配句对
     Study results given by K.
     日本的K.
短句来源
     A Trip to Japan
     游走日本
短句来源
     STUDIES ON THE PATHOGENIC FUNGUS OF PEAR CANKER DISEASE
     梨树腐烂病病原菌的研究
短句来源
     The metabolism of reactive oxygen species in pear (Pyrus bretschneideri Rehd.) flower buds changes greatly during their natural dormancy in winter.
     梨树(Pyrus bretschneideri Rehd.)
短句来源
     Variety Improvement of Pear in Hebei Province
     河北省梨树品种改良
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  japanese pear
Varietal differences were detected in a group of glycoproteins having Mrs and pIs similar to those of the S-glycoproteins of Japanese pear.
      
These glycoproteins reacted with antiserum raised against the S4-glycoprotein of Japanese pear, a result suggesting that they may be the products of S-alleles in styles of apple.
      
PCR-based method for identifying the S-genotypes of Japanese pear cultivars
      
Japanese pear (Pyrus pyrifolia Nakai), a member of the Rosaceae, shows gametophytic self-incompatibility that is controlled by the S-locus.
      
The S-genotype of Japanese pear cultivars is an important factor for crossing and breeding.
      
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A pollen cDNA library of Japanese pear (Pyrus pyrifolia ) of the family Rosaceae was constructed and a cDNA (bgn-1) of 1 408 bp, which encodes a protein (BGN-1) secreted from growing pollen tubes, has been cloned and sequenced. bgn-1 cDNA is composed of a 5'-untranslated region of 47 bp, an open reading frame of 1 194 bp encoding 397 amino acid residues and a complete 3'-untranslated region of 167 bp. Alignment of the deduced amino acid sequence of bgn-1 with that of the barley (Hordeum vulgare )β-1,3-glucanase...

A pollen cDNA library of Japanese pear (Pyrus pyrifolia ) of the family Rosaceae was constructed and a cDNA (bgn-1) of 1 408 bp, which encodes a protein (BGN-1) secreted from growing pollen tubes, has been cloned and sequenced. bgn-1 cDNA is composed of a 5'-untranslated region of 47 bp, an open reading frame of 1 194 bp encoding 397 amino acid residues and a complete 3'-untranslated region of 167 bp. Alignment of the deduced amino acid sequence of bgn-1 with that of the barley (Hordeum vulgare )β-1,3-glucanase (GⅡ) showed 39.7% of amino acid identity. Several residues that were critical for the function of GⅡ were conserved in the deduced BGN-1 polypeptide. Moreover, a hydrophobic cluster analysis (HCA) showed an overall HCA homology score of 87.1% and analysis of BGN-1 with the 3D-PSSM program predicted a 3D structure of BGN-1 highly homologous to that of barley GⅡ with ≥95% certainty. These results suggest that the cloned bgn-1 cDNA encode a β-1,3-glucanase-like protein in Japanese pear. The predicted mature protein (375 aa) has a theoretical molecular mass of 40 723 D, a basic pI of 9.59, a diagnostic amino acid residue mode of D, L, S and L which is very similar to that of growth-related subfamily D (D, L, S and Q) in cereal. A ProXXPro repeat is also found between the position 352 and 367 in the C-extension region.

构建了日本梨树(Pyruspyrifolia)花粉cDNA文库, 发现了一个编码花粉管分泌的类β-1,3-葡聚糖酶蛋白(BGN-1)的cDNA(bgn-1 )并测定了其序列。bgn-1由1408 bp组成, 包括47 bp的5’-非翻译区, 由1194 bp组成并编码了397个氨基酸的ORF和167 bp的3'-非翻译区。3'-非翻译区含有1个NUE(AATTAA)和3个似FUE的基序, 分别位于1369~1374(AAT-TAA)、1320~1325、1327~1332(TTTTTA)和1363~1368(TTTGGA)。 bgn-1编码的蛋白(BGN-1)与DDBJ中的植物β-1,3-葡聚糖酶的序列相似性范围为37%~59%, 与3D结构已知的大麦β-1, 3-葡聚糖酶(GⅡ)的序列相似性为39.7%。对GⅡ与BGN-1进行的疏水簇分析(HCA同源分为87.1%, >75%的一般标准)和使用3D-PSSM软件进行的分析暗示, BGN-1的3D结构与大麦GⅡ同源性最大(≧95%的肯定度)。BGN-1的信号肽长度为22 aa, 成熟的BGN-1(375 aa)的理论分子量为4 0723 D, ...

构建了日本梨树(Pyruspyrifolia)花粉cDNA文库, 发现了一个编码花粉管分泌的类β-1,3-葡聚糖酶蛋白(BGN-1)的cDNA(bgn-1 )并测定了其序列。bgn-1由1408 bp组成, 包括47 bp的5’-非翻译区, 由1194 bp组成并编码了397个氨基酸的ORF和167 bp的3'-非翻译区。3'-非翻译区含有1个NUE(AATTAA)和3个似FUE的基序, 分别位于1369~1374(AAT-TAA)、1320~1325、1327~1332(TTTTTA)和1363~1368(TTTGGA)。 bgn-1编码的蛋白(BGN-1)与DDBJ中的植物β-1,3-葡聚糖酶的序列相似性范围为37%~59%, 与3D结构已知的大麦β-1, 3-葡聚糖酶(GⅡ)的序列相似性为39.7%。对GⅡ与BGN-1进行的疏水簇分析(HCA同源分为87.1%, >75%的一般标准)和使用3D-PSSM软件进行的分析暗示, BGN-1的3D结构与大麦GⅡ同源性最大(≧95%的肯定度)。BGN-1的信号肽长度为22 aa, 成熟的BGN-1(375 aa)的理论分子量为4 0723 D, 理论pI值为9.59, 诊断氨基酸残基模式(D, L, S, L)与禾谷类的与生长相关的亚家族D的诊断氨基酸残基模式(D, L, S, Q)极相似。在BGN-1的C-端延伸区的位点352~367之间发现了1个功能不清, 重复出现的ProXXPro结构。

 
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