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转化育种
相关语句
  transformation breeding
     STUDIES ON THE TRANSFORMATION BREEDING OF HIGH INOSION PRODUCING STRAIN TSXB29 AND ITS FERMENTABLE CONDITIONS
     肌苷高产菌TSXB29的转化育种及其发酵条件的研究
短句来源
     So far,applying nucleus genetic transformation system for poplar improvement is popular while chloroplast genetic transformation system almost has not been employed for poplar genetic transformation. Based on the development of chloroplast genetic transformation technique and the coming requirement of poplar breeding,it could improve in certain degree the effects of poplar genetic transformation breeding in China to try in time to develop poplar chloroplast genetic transformation.
     目前,杨树遗传转化时大多应用的都是核遗传转化系统,几乎还没有人进行过其叶绿体的遗传转化,参照目前叶绿体遗传转化技术的发展水平及杨树遗传转化育种发展的需要,及时尝试开展杨树叶绿体遗传化的研究可能会在一定程度上提高我国杨树遗传转化育种的效果。
短句来源
  “转化育种”译为未确定词的双语例句
     Transformation of High Inosine-producing Strain and Application of Uniform-design in Shaking Culture
     肌苷高产菌的转化育种及均匀设计在发酵条件试验中的应用
短句来源
     STUDY ON THE ELECTROTRANSFORMATION TECHNOLOGY OF AGARICUS BISPORVS
     双孢蘑菇电击转化育种技术研究
     Five breeding methods including transgression breeding, trans gene breeding, applying heterosis in apomixis, manipulating phenology breeding and improving physiological properties breeding were raised too.
     并提出了常规超亲育种、基因转化育种、一系法利用杂种优势、操纵物候期育种和生理特性改良育种等 5种超级小麦育种方法
短句来源
  相似匹配句对
     First pET-hαCGRP was transformed to E.
     转化E.
短句来源
     After E.
     转化E.
短句来源
     Mutation Breeding of 4AD Producing Strain by UV and Laser Irradiation
     雄甾烯二酮转化菌的诱变育种
短句来源
     Haploid Breeding and Gene Transformation of Maize
     玉米的单倍体育种及基因转化的研究
短句来源
     P ig Breeding
     猪的育种
短句来源
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Interspecific trrnsformants have been obtained with Bacillus subtilis AS1—398—28—6 (Arg~- Leu~- Rif~r Str~s) and B. licheniformis 1—807—9—9 (thr~- Ade~-Str~s Rif~r). The starting strains of parent above are being used for produing enzgmes in China, As 1—398 produces neutral protease and the others produces alkaline protease. The protoplast for transformation is prepared with lysozyme. The optimum conditions for preparing protpolast are lysozyme 250 mg/ml, pH 6.7~7.0, temperature 35℃ in SMM. The rate of breaking...

Interspecific trrnsformants have been obtained with Bacillus subtilis AS1—398—28—6 (Arg~- Leu~- Rif~r Str~s) and B. licheniformis 1—807—9—9 (thr~- Ade~-Str~s Rif~r). The starting strains of parent above are being used for produing enzgmes in China, As 1—398 produces neutral protease and the others produces alkaline protease. The protoplast for transformation is prepared with lysozyme. The optimum conditions for preparing protpolast are lysozyme 250 mg/ml, pH 6.7~7.0, temperature 35℃ in SMM. The rate of breaking wall of Cells is 99.99% and of regeneration 1~4%. The reverse frequency of donor and receptor for auxotroph and resistance is less than 10~(-9), protoplast transformation is carried out in SMM containing 30% oolyethene glycol (MW 6000) and 20mg/ml DNA at controtledtime and temperature. The transformation frequency is about 10~(-2)~10~(-5) which dependes on the rate of regeneration and the concentration of DNA when fixed other conditions.The stable rate of transformants through the first transfer is 40%. The transformants could grow in minimal medium added streptomyces 200 unit/ml and rifampin (10 unit/ml). The shape of cells of transformants is longer, long and short. The character of conditional sporatatiort of transformants have some variety. The donor is no or less sporulation and the receptor is more but some of transformants are less sporalation and some are more. Receptor could secrete red pigment in CM containing glucose and donor could not, but some of transformants are could not and some are could, these results might be to tell us that the protoplast transformation is not only a new way for transformation breeding but might be integrate longer fragment of DNA or more group of genes into protoplast.

以地衣形芽抱杆菌(Bacillus licheniformis)AS 1.807—9—9(Thr~-Ade—Str~rRif~5)的原土质体为受体,枯草芽抱杆菌(B.subtilis)ASl.398—28—6(Arg~-Leu~-Rif~rStr~s)*为供体菌,添加聚乙二醇(分子量6000)至终浓度为30%,经过一定时间的保温处理,成功地获得了芽孢杆菌的种间转化子。转化子有的是原养型,有的在含利福平(10单位/亳升)和链霉素(200单位/亳升)的培养基上能生长,也有的兼而有之。上述标记的转化频率在10~(-2)~10~(-5)之间。转化子的产芽孢性能和在含葡萄糖的完全培养基上分泌红色色素的性能亦各有差异,而受体菌是典型产芽孢和分泌红色色素的,供体菌是寡芽孢产株,亦不分泌红色色素。溶菌酶处理时间不同致使原生质体再生率有改变或供体DNA添加量不同都会影响转化频率。转化子的次代回变率约大于60%。原生质体转化可以是转化育种的一种新途径,原生质体也可能是经体外重组DNA的植入受体。

The fungus disease resistant rape breeding system was established b y infecting the stalk cotyledon and hypocotyl through Agrobacterium tumefacien s with the bivalent carrier plasmid which contains the β -1,3-glucanase gene and t he chitinase gene.The influences of different hormones of different concentrati ons on induction of adventitious buds were researched.The research results indi cated as the following.Through the selection of adding kanamycin,the best leve l of the emergence rate and green...

The fungus disease resistant rape breeding system was established b y infecting the stalk cotyledon and hypocotyl through Agrobacterium tumefacien s with the bivalent carrier plasmid which contains the β -1,3-glucanase gene and t he chitinase gene.The influences of different hormones of different concentrati ons on induction of adventitious buds were researched.The research results indi cated as the following.Through the selection of adding kanamycin,the best leve l of the emergence rate and green plant differentiation rate of the stalk cotyle dons cultured on the MS medium with 6-BA of 6?mg/L were 100% and 25% respectiv ely ;that of the hypotocyls cultured in the MS medium with 6-BA of 1?mg/L and 2,4- D of 0.1?mg/L were 100% and 23% respectively.The differentiated rape green plan ts were transplanted into the flowerpot and selfing.The hypocotyl cotyledons of se lfed germ free plants were selected in the MS medium with the kanamycin of 50? m?g/L,and the little plants were all survival.The test results prelimin arily prov ed the purpose genes were transferred into the Brassica napus plants and the 2 kanamycin selections of inducing bearing buds and roots were effective.

用含β-1,3-葡聚糖酶和几丁质酶串联基因双价载体质粒PBLGC的根癌农杆菌侵染甘蓝型油菜(Brasicanapus)带柄子叶和下胚轴的方法,初步建立了抗真菌病害油菜高效基因转化育种体系.研究了不同种类和不同浓度的激素对带柄子叶和下胚轴不定芽诱导的影响,试验结果表明经卡那霉素(kan)筛选,含6mg/L6-BA的MS培养基中带柄子叶的出苗率及绿苗分化率最高,分别为100%和25%;下胚轴在含1mg/L6-BA和0.1mg/L2,4-D的MS培养基中出芽率及绿苗分化率最高,分别为100%和23%.绿苗在含50mg/Lkan的诱根培养基中培养后移栽于花盆,套袋自交,自交种子所得无菌苗的带下胚轴子叶再经含50mg/Lkan的MS培养基筛选培养,结果小苗全部生根成活,初步证明目的基因已转入油菜植株中,经诱导不定芽阶段和生根阶段两次kan筛选是有效的

The conception of super wheat and the breeding targets were raised. The feasibility of super wheat breeding was discussed according to the potential in super high yield, examples of high yield, special resource of germplasm and the relationship between yield and quality. Five breeding methods including transgression breeding, trans gene breeding, applying heterosis in apomixis, manipulating phenology breeding and improving physiological properties breeding were raised too.

本文提出了超级小麦的概念和指标 ;从小麦的超高产潜力、高产典型、特异育种资源及产量与品质的关系等方面 ,论证了超级小麦育种的可行性 ;并提出了常规超亲育种、基因转化育种、一系法利用杂种优势、操纵物候期育种和生理特性改良育种等 5种超级小麦育种方法

 
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