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cdna斑点杂交
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  cdna dot hybridization
     Results An efficient subtractive library of DP of lesional follicles from alopecia areata was established. A differentially expressed gene, DP3 (307 bp), was successfully cloned. cDNA dot hybridization and Northern hybridization proved the gene was expressed specifically only in DP of lesional follicles, and homology search indicated that this gene was a thyroid related autoantigen gene.
     结果 成功建立了高消减效率的斑秃脱发区毛乳头cDNA消减文库 ,并克隆到一条斑秃毛乳头特异表达的基因片段DP3( 3 0 7bp) ,应用cDNA斑点杂交及Northern杂交证实它仅在脱发区毛乳头特异表达 ,同源性检索其为一条甲状腺相关的自身抗原基因。
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  “cdna斑点杂交”译为未确定词的双语例句
     Low molecular mass RNAs extracted from bark of one year old branch of 24 apricot samples,37 plum samples were used for DIG-cDNA Dot-blot hybridization,RT-PCR,Return-PAGE and biological indexing. There were no clear symptoms when these samples were collected.
     从无明显症状表现的24个杏样品,37个李样品的1年生枝条表皮中抽提低分子RNA进行DIG-cDNA斑点杂交、RT-PCR、正反向聚丙烯酰胺凝胶电泳(Return-PAGE)和生物学检测。
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  相似匹配句对
     RNA dot hy- bridization.
     RNA斑点杂交
短句来源
     Experimental study Detecting Entroviruses RNA With the Method of cDNA: RNA Spot Hybridization
     用cDNA:RNA斑点杂交法检测肠道病毒RNA的实验研究
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     Method: Dot hybridization.
     方法:利用斑点杂交分析方法。
短句来源
     THE PREPARATION OF BIOTIN-LABELED HDV cDNA PROBE AND ITS APPLICATION TO SPOT HYBRIDIZATION
     生物素化HDV cDNA探针的制备及其在血清斑点杂交中的应用
短句来源
     Dot blots of total RNAs were hybridized with appropriate cDNA probes measurement of the mRNA level for uPA and uPAR in treated SGC7901.Results:After treatment with E2 (0.1 μmol/L) alone, SGC7901 cells uPA and uPAR mRNA expression levels increased about 2.9or 2.1fold respectively.
     以cDNA—mRNA斑点杂交分析受处理的肿瘤细胞uPA及uPARmRNA表达水平。
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A virus isolate (w) has been obtained from the Oker river in W.Germany. The isolate was identified as Carnation ringspot virus (CarRSV) by means of inoculation on the test plant, electron microscopy, dot blot hybridization and serology.The molecular weight of the coat protein is 3.8×10~4. Northern blot analysis revealed that the two RNA species of the virus which consisted of c. 3.7 and 1.5kb, respectively, but without base sequence homology.

在联帮德国奥卡河中分离到一种病毒分离物(W),通过鉴别寄主反应的测定,病毒粒子的电子显微镜观察,cDNA斑点杂交和血清学的研究,鉴定出这一病毒分离物为香石竹环斑病毒。其外壳蛋白亚基的分子量为3.8×10~4。cDNA吸印转移杂交分析表明,香石竹环斑病毒的两个RNA组份(RNA~1、RNA_2)之间没有核苷酸序列同源性。RNA_1和RNA_2分别由3.7和1.5仟碱基组成。

Objective To construct a differentially expressed genes subtracted cDNA library of dermal papillae cells(DPC) with aggregative behavior Methods Total RNA was extracted from DPC with and without aggregative behavior and double strand cDNA was synthesized by using SMART cDNA synthesis, respectively The cDNA fragments of differentially expressed genes in dermal papillae cells with aggregative behavior were isolated by suppression subtractive hybridization and then directly inserted into T/A cloning vector...

Objective To construct a differentially expressed genes subtracted cDNA library of dermal papillae cells(DPC) with aggregative behavior Methods Total RNA was extracted from DPC with and without aggregative behavior and double strand cDNA was synthesized by using SMART cDNA synthesis, respectively The cDNA fragments of differentially expressed genes in dermal papillae cells with aggregative behavior were isolated by suppression subtractive hybridization and then directly inserted into T/A cloning vector to set up the subtracted library Verification of the insert fragment was performed on 100 positive clones randomly screened by PCR method and among which 30 clones with insert fragments were verified by cDNA dot blot Results The amplified library contained approximately 320 positive bacteria clones Random analysis of 100 clones with nested PCR method showed that 95% clones contained 100~600 bp inserts cDNA dot blot showed that 27 clones (90%) were positive Conclusion A differentially expressed genes subtracted cDNA library of dermal papillae cells(DPC) with aggregative behavior is constructed by suppression subtractive hybridization combining with SMART cDNA synthesis The library is a highly efficient one and lays solid foundation for screening and cloning new and specific genes related to aggregative behavior of DPC

目的 构建毛乳头细胞凝集性生长状态下差异表达基因的消减cDNA文库。方法 分别提取凝集性生长和非凝集性生长状态下毛乳头细胞中的总RNA ,应用SMARTcDNA合成技术和抑制性消减杂交技术分离毛乳头细胞凝集性生长状态下差异表达基因的cDNA片段并建立消减cDNA文库 ;利用PCR对随机挑选的 10 0个白色菌落进行插入片段的验证 ,对其中证实有插入片段的 3 0个克隆进行cDNA斑点杂交验证。结果 所构建的消减cDNA文库扩增后得到 3 2 0个阳性克隆 ,随机挑选的 10 0个阳性克隆中 95 %的均有长度在 10 0~ 60 0bp的插入片段 ,cDNA斑点杂交验证显示 2 7个( 90 0 % )克隆为阳性。结论 结合SMARTcDNA合成技术 ,应用抑制性消减杂交成功地构建了毛乳头细胞凝集性生长差异表达基因消减cDNA文库 ,为进一步筛选和克隆毛乳头细胞凝集性生长相关基因奠定了基础

Objective To screen and analyze genes differentially expressed within dermal papillae cells(DPC)with aggregative behavior.Methods Total RNA was extracted from DPC with and without ag-gregative behavior,and double-stranded cDNA were synthesized by using SMART cDNA synthesis.The cD-NA fragments of differentially expressed genes in dermal papillae cells with aggregative behavior were isolat-ed by suppression subtractive hybridization,sequencing,and then subtracted library was set up.Positive clones were screened...

Objective To screen and analyze genes differentially expressed within dermal papillae cells(DPC)with aggregative behavior.Methods Total RNA was extracted from DPC with and without ag-gregative behavior,and double-stranded cDNA were synthesized by using SMART cDNA synthesis.The cD-NA fragments of differentially expressed genes in dermal papillae cells with aggregative behavior were isolat-ed by suppression subtractive hybridization,sequencing,and then subtracted library was set up.Positive clones were screened by PCR method and verified by cDNA dot blot and then analyzed through homologous retrieving.Results A subtractive cDNA library of DPC with aggregative behavior was successfully construct-ed.The results of screening and cloning of the library showed that DPC with aggregative behavior could ex-press genes related to homologous aggregation,regnlation of growth,differentiation and development,and sig-nal transduction proliferation and cycle control,which included known genes(capping protein,paladin,vas-cular endothelial growth factor),hematopoietic stem/progenitor cells(HSPC)related genes(HSPC011and HSPC016)and a new gene.Conclusions The construction of subtractive library of DPC lays solid founda-tion for screening and cloning new and specific genes related to aggregative behavior of DPC.Several genes may cooperatively involve in homologous aggregation,and regnlation of growth of DPC.Among these genes,capping protein and palladin may be closely related to aggregative behavior of DPC,and VEGF and HSPC re-lated clones may be responsible for the status of higher proliferation of DPC.

目的筛选和分析毛乳头细胞凝集性生长状态下差异表达基因。方法分别提取凝集性生长和非凝集性生长状态下毛乳头细胞中的总RNA,应用SMARTcDNA合成技术和抑制性消减杂交技术分离毛乳头细胞凝集性生长差异表达基因的cDNA片段,并构建消减cDNA文库,通过PCR筛选、cDNA斑点杂交验证、测序和同源性检索对差异表达基因进行分析。结果成功地构建了毛乳头细胞凝集性生长差异表达cDNA文库。通过对毛乳头细胞凝集性生长状态差异表达基因文库的筛选、克隆,证实毛乳头在体外凝集性生长状态下可以特异表达与细胞同源凝集、生长调控、分化发育及信号转导相关的基因,包括已知基因(如加帽蛋白、palladin,VEGF)、造血干细胞分化相关基因(HSPC011、HSPC016)及1条新基因。结论毛乳头细胞凝集性生长差异表达基因消减cDNA文库的构建,为进一步筛选和克隆毛乳头细胞凝集性生长相关基因奠定了基础。在凝集性生长状态下,多种基因可能协同作用,参与毛乳头细胞生长调控和功能发挥。

 
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