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个血液肿瘤细胞株
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  haematological tumor cell lines
     Objective To develop a real-time reverse transcription polymerase chain reaction(RT-PCR) for the relative quantitation of PAX5 and CD19 mRNA expression in 6 haematological tumor cell lines.
     目的 建立实时定量逆转录 聚合酶链反应(RT PCR)检测6个血液肿瘤细胞株中PAX5和CD19的 mRNA相对表达量。
短句来源
     Methods A real-time RT-PCR assay for the relative quantitation of PAX5 and CD19 mRNA expression was developed and applied on 6 haematological tumor cell lines and bone marrow cells of 6 normal children,58 previously untreated and 4 relapse acute leukemic children.
     方法采用实时定量RTPCR方法,检测了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童骨髓细胞中PAX5和CD19mRNA的表达水平。
短句来源
     BSAP expression by Western Blotting analysis was performed in haematological tumor cells,including 6 haematological tumor cell lines and 4 clinical samples.
     采用WesternBlotting分析了6个血液肿瘤细胞株和4个临床样本中BSAP表达水平。
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  “个血液肿瘤细胞株”译为未确定词的双语例句
     To investigate transcription factor PAX5 expression characteristics in childhood acute leukemic cells, expression levels of PAX5 and CD19 mRNA in 6 hematological tumor cell lines and bone marrow cells of 6 normal children, 58 de novo patients and 4 relapse acute leukemic children, including 39 cases of B-ALL,10 cases of T-ALL and 13 cases of AML, were detected by a real-time RT-PCR.
     为了观察了解儿童急性白血病细胞中转录因子PAX5的表达特性,采用实时定量RT-PCR方法测定了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童(其中包括39例B-ALL,10例T-ALL和13例AML)骨髓细胞中PAX5和CD19mRNA的表达水平。
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  相似匹配句对
     Blood Substitute
     血液代用品
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     The Fishes Blood
     鱼类的血液
短句来源
     A Study on the Blood Cell of Anguilla japonica
     鳗鲡血液细胞的研究
短句来源
     Relationship between malignant disease and hemostatic system
     恶性肿瘤血液系统
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     The identity between Shandong N.
     b和广东N .
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Objective To develop a real-time reverse transcription polymerase chain reaction(RT-PCR) for the relative quantitation of PAX5 and CD19 mRNA expression in 6 haematological tumor cell lines. Methods A serial dilution cDNA reversely transcriped from total RNA in NAMALWA were used to construct standard curves for the PAX5, CD19 and GAPDH amplifications. The amplification efficiencies were identical for both the target gene(PAX5 and CD19) and house keeping gene(GAPDH). Results PAX5 and CD19 mRNA expression...

Objective To develop a real-time reverse transcription polymerase chain reaction(RT-PCR) for the relative quantitation of PAX5 and CD19 mRNA expression in 6 haematological tumor cell lines. Methods A serial dilution cDNA reversely transcriped from total RNA in NAMALWA were used to construct standard curves for the PAX5, CD19 and GAPDH amplifications. The amplification efficiencies were identical for both the target gene(PAX5 and CD19) and house keeping gene(GAPDH). Results PAX5 and CD19 mRNA expression level was 2.35% and 2.52% respectively in NAMALWA(B-cell lines), but almost not detectable in other T-and myeloid cell lines. Conclusions The real-time RT-PCR is simple, rapid and convenient for quantification of PAX5 and CD19 mRNA levels, and is suitable for investigation in larger groups of patients with different haematological malignancies.

目的 建立实时定量逆转录 聚合酶链反应(RT PCR)检测6个血液肿瘤细胞株中PAX5和CD19的 mRNA相对表达量。方法 一系列稀释的反转录自NAMALWA细胞株总RNA的cDNA被用于构建PAX5、CD19 和GAPDH扩增的标准曲线。实验证实靶基因(PAX5和CD19)与管家基因(GAPDH)的扩增效率一致,因此可 以用比较循环数(Ct)法对PAX5和CD19的表达进行相对定量。结果 在NAMALWA(B细胞系)细胞株中, PAX5和CD19mRNA相对表达量分别为2.35%和2.52%;而在其他T细胞和髓系细胞株中几乎不表达。结论  本研究中所建立的实时定量RT PCR能简单、快速、方便地对PAX5和CD19mRNA表达水平进行定量,且适 用于对大量、不同种血液恶性肿瘤患者进行观察研究。

Objective To investigate transcription factor PAX5/BSAP expression characteristics in childhood acute leukemic cells and haematolological tumor cell lines.Methods A real-time RT-PCR assay for the relative quantitation of PAX5 and CD19 mRNA expression was developed and applied on 6 haematological tumor cell lines and bone marrow cells of 6 normal children,58 previously untreated and 4 relapse acute leukemic children.BSAP expression by Western Blotting analysis was performed in haematological tumor cells,including...

Objective To investigate transcription factor PAX5/BSAP expression characteristics in childhood acute leukemic cells and haematolological tumor cell lines.Methods A real-time RT-PCR assay for the relative quantitation of PAX5 and CD19 mRNA expression was developed and applied on 6 haematological tumor cell lines and bone marrow cells of 6 normal children,58 previously untreated and 4 relapse acute leukemic children.BSAP expression by Western Blotting analysis was performed in haematological tumor cells,including 6 haematological tumor cell lines and 4 clinical samples.RNAi(RNA interference) was used to specifically block PAX5 expression in NAMALWA(B-cell lines).Results PAX5 and CD19 mRNA expression level was 2.35%, 2.52%, respectively,in NAMALWA,but almost not detectable in other T- and myeloid cell lines.The results of Western Blotting analysis showed a 52-KD BSAP band in B lineage cells,but not in T- and myeloid lineage cells.Among clinical samples,expression of PAX5 mRNA in B-ALL(acute B-lineage lymphoblastic leukemia) was significantly higher than that in T-ALL(acute T-lineage lymphoblastic leukemia) and AML(acute myeloid leukemia)(P=0.029 and P=0.013,respectively).Individual difference of PAX5 mRNA expression levels in children with B-ALL was great.PAX5 suppression in NAMALWA by RNAi was 35%,but had no effect on cell proliferation(P<0.05).Conclusions The strong PAX5 mRNA expression in some B-ALL can be considered to be particularly interesting for further analysis.RNAi is also suitable for further investigation in B-lineage haematological tumor cells.

目的了解儿童急性白血病细胞和血液肿瘤细胞株中转录因子PAX5/BSAP的表达特性。方法采用实时定量RTPCR方法,检测了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童骨髓细胞中PAX5和CD19mRNA的表达水平。采用WesternBlotting分析了6个血液肿瘤细胞株和4个临床样本中BSAP表达水平。采用RNAi(RNA干扰)技术特异性阻断NAMALWA细胞株(B细胞系)中PAX5的表达。结果在NAMALWA细胞株中,PAX5和CD19mRNA相对表达量分别为2.35%和2.52%;而在T和髓系细胞株中几乎不表达。同时运用WesternBlotting在B系细胞中检测到一52KD的BSAP条带,但在T和髓系细胞中为阴性。在临床样本中BALL(急性B淋巴细胞白血病)组比TALL(急性T淋巴细胞白血病)组和AML(急性髓细胞性白血病)组的PAX5mRNA表达高(P=0.029和P=0.013)。在BALL患儿中,PAX5mRNA表达的个体差异很大。RNAi技术抑制NAMALWA细胞株中PAX5基因表达达35%,但不影响细胞增殖效率(P<0.05)。结论部分BAL...

目的了解儿童急性白血病细胞和血液肿瘤细胞株中转录因子PAX5/BSAP的表达特性。方法采用实时定量RTPCR方法,检测了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童骨髓细胞中PAX5和CD19mRNA的表达水平。采用WesternBlotting分析了6个血液肿瘤细胞株和4个临床样本中BSAP表达水平。采用RNAi(RNA干扰)技术特异性阻断NAMALWA细胞株(B细胞系)中PAX5的表达。结果在NAMALWA细胞株中,PAX5和CD19mRNA相对表达量分别为2.35%和2.52%;而在T和髓系细胞株中几乎不表达。同时运用WesternBlotting在B系细胞中检测到一52KD的BSAP条带,但在T和髓系细胞中为阴性。在临床样本中BALL(急性B淋巴细胞白血病)组比TALL(急性T淋巴细胞白血病)组和AML(急性髓细胞性白血病)组的PAX5mRNA表达高(P=0.029和P=0.013)。在BALL患儿中,PAX5mRNA表达的个体差异很大。RNAi技术抑制NAMALWA细胞株中PAX5基因表达达35%,但不影响细胞增殖效率(P<0.05)。结论部分BALL中PAX5mRNA表达明显升高,可作为进一步研究BALL发病机理的另一个切入点。RNAi技术可进一步用于B系血液恶性肿瘤细胞的观察研究。

To investigate transcription factor PAX5 expression characteristics in childhood acute leukemic cells, expression levels of PAX5 and CD19 mRNA in 6 hematological tumor cell lines and bone marrow cells of 6 normal children, 58 de novo patients and 4 relapse acute leukemic children, including 39 cases of B-ALL,10 cases of T-ALL and 13 cases of AML, were detected by a real-time RT-PCR. The results showed that PAX5 and CD19 mRNA expression levels were 2.35% and 2.52% in Namalwa (B-cell lines) respectively, but almost...

To investigate transcription factor PAX5 expression characteristics in childhood acute leukemic cells, expression levels of PAX5 and CD19 mRNA in 6 hematological tumor cell lines and bone marrow cells of 6 normal children, 58 de novo patients and 4 relapse acute leukemic children, including 39 cases of B-ALL,10 cases of T-ALL and 13 cases of AML, were detected by a real-time RT-PCR. The results showed that PAX5 and CD19 mRNA expression levels were 2.35% and 2.52% in Namalwa (B-cell lines) respectively, but almost not detectable in other T- and myeloid cell lines. Among clinical samples, expression of PAX5 mRNA in B-ALL was significantly higher than that in T-ALL and AML(P=0.029 and P=0.013 respectively). PAX5 expression was significantly lower in T-ALL and AML than that in normal controls.The difference of PAX5 mRNA expression levels between T-ALL and AML was not significant. Individual difference of PAX5 mRNA expression levels in children with B-ALL was great. Moreover, PAX5 mRNA expressions in de novo and relapse patients with B-ALL were significantly higher than those in remission (P=0.011 and P=0.006 respectively). As binding sites for B-cell specific activator protein have been identified in the promoter regions of CD19, the study found that in B-ALL, there was clear correlation between the expression levels of PAX5 and CD19, which was also studied by real-time RT-PCR. It is concluded that PAX5 transcripts are readily detectable and quantifiable in clinical materials with B-ALL by real-time RT-PCR. The strong PAX5 mRNA expression in some B-ALL can be considered to be particularly interesting for further analysis.

为了观察了解儿童急性白血病细胞中转录因子PAX5的表达特性,采用实时定量RT-PCR方法测定了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童(其中包括39例B-ALL,10例T-ALL和13例AML)骨髓细胞中PAX5和CD19mRNA的表达水平。结果表明:在Namalwa(B细胞系)细胞株中,PAX5和CD19mRNA相对表达量分别为2.35%和2.52%;而在T-和髓系细胞株中几乎不表达。在临床样本中B-ALL组比T-ALL组和AML组的PAX5mRNA表达高(P=0.029和P=0.013)。T-ALL组和AML组的PAX5mRNA表达水平均低于正常对照组,而T-ALL组与AML组之间的表达差异无显著意义。在B-ALL患儿中,PAX5mRNA表达的个体差异很大。此外还发现,初发治疗前组和复发组的B-ALL患儿PAX5mRNA表达高于化疗完全缓解组(P=0.011和P=0.006)。由于在CD19基因的启动子上有B细胞特异性激活蛋白的结合位点,研究发现在B-ALL中PAX5表达水平与同样用实时定量RT-PCR检测的CD19表达水平之间存在明显的相关性。结论:运用实时定量...

为了观察了解儿童急性白血病细胞中转录因子PAX5的表达特性,采用实时定量RT-PCR方法测定了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童(其中包括39例B-ALL,10例T-ALL和13例AML)骨髓细胞中PAX5和CD19mRNA的表达水平。结果表明:在Namalwa(B细胞系)细胞株中,PAX5和CD19mRNA相对表达量分别为2.35%和2.52%;而在T-和髓系细胞株中几乎不表达。在临床样本中B-ALL组比T-ALL组和AML组的PAX5mRNA表达高(P=0.029和P=0.013)。T-ALL组和AML组的PAX5mRNA表达水平均低于正常对照组,而T-ALL组与AML组之间的表达差异无显著意义。在B-ALL患儿中,PAX5mRNA表达的个体差异很大。此外还发现,初发治疗前组和复发组的B-ALL患儿PAX5mRNA表达高于化疗完全缓解组(P=0.011和P=0.006)。由于在CD19基因的启动子上有B细胞特异性激活蛋白的结合位点,研究发现在B-ALL中PAX5表达水平与同样用实时定量RT-PCR检测的CD19表达水平之间存在明显的相关性。结论:运用实时定量RT-PCR方法能快速准确地在B-ALL的临床标本中检测和定量PAX5基因的表达。研究中发现部分B-ALL中PAX5mRNA表达明显升高,因此它可将其作为进一步研究B-ALL发病机理的另一个切入点。

 
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