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分析细胞
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  analyzing cell
     The CHO cell lines stably overexpressing MnSOD and GPx were used asthe cell models to compare the different responses of MnSOD and GPx on tBOOH and Paraquat inducedcell death by analyzing cell survival and mitochondrial function.
     本研究以稳定表达MnSOD与GPx的CHO细胞系为细胞模型,通过分析细胞存活能力和线粒体功能比较研究了MnSOD与GPx对tBOOH和Paraquat诱导细胞死亡的不同影响。
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     Using fluorescence activated cell sorter (FACS) for: (1 ) detecting cell apoptosis with Annexin V; (2)analyzing cell cycle by propidium iodide (Pl )staining.
     应用FACS:(1)AnnexinV法检测细胞凋亡,(2)PI染色分析细胞周期。
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  “分析细胞”译为未确定词的双语例句
     Cell cycle analysis by FACS showed that 801D-FHIT,A2FHIT and A549-FHIT cells were blocked at G_1,S and G_2 phase,respectively.
     FACS分析细胞周期显示:801D-FHIT、A2-FHIT和A549-FHIT细胞分别出现了G1、S和G2/M期阻滞。
短句来源
     DCs were interfered by recombination human IL-10. HLA-DR, CD11a, CD11c, CD83 , CD80 and CD86 was assayed by flow cytometry.
     流式细胞仪分析细胞表型CD1a、CD11c、CD83、CD80、CD86和HLA-DR;
短句来源
     DCs were interfered by PsL-EGFmAb and IL-10. HLA-DR, CD1a, CD11c, CD83, CD80 and CD86 was assayed by flow cytometry.
     采用流式细胞仪分析细胞表型CD1a、CD11c、CD83、CD80、CD86和HLA-DR;
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     zymogram analysis was used to analyze the activity of MMP-2 and MMP-9. Results (1)In the process of stimulation by TGF-β_1,the type Ⅰ collagen mRNA level of 24 h,48 h and 72 h group was:1.33±0.07,2.46±0.09 and 2.39±0.08 respectivley;
     酶谱分析细胞上清液中MMP-2、MMP-9的活力。 结果(1)TGF-β1刺激HLF-02细胞,244、8、72 h组的Ⅰ型胶原mRNA表达水平分别为1.33±0.07、2.46±0.09、2.39±0.08;
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     Methods:Spleen and thymus T cells were separated and FACS assayed the expression of CD3 、CD8、CD4 from mice 3 to 9 weeks of age;
     方法 :分离 3~ 9周小鼠胸腺、脾脏T淋巴细胞 ,FACS分析细胞表面CD3、CD4、CD8的表达 ;
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  相似匹配句对
     C, methylation analysis etc were used.
     分析,G. C.
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     This paper analyses the development background of g.
     分析了g.
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     CTanalysisofcerebrospinalfluidcelcomponents
     脑脊液细胞成分的CT分析
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     MRI Analysis of Renal Cell Carcinoma
     肾细胞癌MRI分析
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  analyzing cell
Traditional and emerging methods for analyzing cell activity in cell culture
      
The enzymatic basis for this bioconversion was evaluated by analyzing cell-free extracts of G.
      
In this paper we describe a novel optical fiber-based technology for analyzing cell migration.
      
Electroporatical introduction of a reporter gene was therefore found to be a suitable method for analyzing cell-specific expression in intact tissues and to be substitute for germ-line transmission of reporters in the transgenic system.
      
By analyzing cell interactions in a quantitative adhesion assay using mechanically dissociated Hydra epithelial cells, we show that aggregation proceeds in two steps.
      
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17 young healthy rabbits weighing from 520 to 720 gins,ageing 38~48 postnatal days,were used for this study.Seven to fourteen days following spinal lesions at diffe- rent levels the animals were killed and serial transverse sections of thc brain stem were stained with thionin,the retrograde cell degenerations within the red nucleus were studied. An analysis of the chromatolysis indicates that the rubrospinal fibers are largely crossed and a few fibers to the cervical cord are uncrossed.Furthermore,a somatotopical...

17 young healthy rabbits weighing from 520 to 720 gins,ageing 38~48 postnatal days,were used for this study.Seven to fourteen days following spinal lesions at diffe- rent levels the animals were killed and serial transverse sections of thc brain stem were stained with thionin,the retrograde cell degenerations within the red nucleus were studied. An analysis of the chromatolysis indicates that the rubrospinal fibers are largely crossed and a few fibers to the cervical cord are uncrossed.Furthermore,a somatotopical localization exists within the contralateral red nucleus with respect to the rubrospinal tract,i.e.the cervical projections of the fibers system take origin from the dorsal part of the red nucleus almost throughout its whole extent,the thoracic projections from the ventral part of the caudal five-sixths of the red nucleus,and the lumbar projections from the centro-ventral part of the caudal third of the nucleus. It also indicates that all types of cells in the red nucleus,not only the large ones, give rise to the rubrospinal tract.The spinal porjections of this fiber tract may descend as far as the lumbar cord.

用健康幼小家兔17只作为实验材料,体重520~720克,生后38~48天,在脊髓不同平面(颈、胸、腰)作半横切或部分半横切,喂养7~14日后杀死,取脑干和手术部脊髓,制连续横切片,厚15微米,硫堇染色。观察红核内的溃变细胞,分析细胞溃变的结果表明:红核脊髓束大部分是交叉性的,只有少量至颈髓的纤维不交叉。交叉性的红核脊髓束在红核的起始有躯体定位。即此束的颈髓投射起自对侧红核的背侧部;胸髓投射起自核尾侧段5/6的腹侧部;腰髓投射起自核尾侧段1/3的中腹部。此外,本实验还证实:不仅红核大型细胞,其它中、小型细胞也发纤维构成红核脊髓束,它可到达腰髓平面。

The estrogen receptor of the rabbit uterine cytosol and the SHBG in a pregnant woman'sserum were analyzed by isoelectrofocusing,using the small glass column of polyacrylamidegel.Under the cooling influence of ice-water and in the presence of free estrogen,the dis-sociation of the steroid-protein complex was minimized.The results showed the isoelectricpoint of the woman's serum SHBG to be 5.2,while the rabbit uterine cytosol estrogen re-ceptor appeared in two peaks at pH 5.8 and 6.8.In the presence of 0.4 M KCL,the...

The estrogen receptor of the rabbit uterine cytosol and the SHBG in a pregnant woman'sserum were analyzed by isoelectrofocusing,using the small glass column of polyacrylamidegel.Under the cooling influence of ice-water and in the presence of free estrogen,the dis-sociation of the steroid-protein complex was minimized.The results showed the isoelectricpoint of the woman's serum SHBG to be 5.2,while the rabbit uterine cytosol estrogen re-ceptor appeared in two peaks at pH 5.8 and 6.8.In the presence of 0.4 M KCL,the peakat pH 6.8 increased and that at pH 5.8 disappeared.Thus,the former might be the 4 Sprotein complex,and the latter the 8 S protein complex.

本文介绍用一种简便、快速的小玻璃管聚丙烯酰胺凝胶等电聚焦来分析细胞浆液雌激素受体及血清性激素结合蛋白的方法。作者采取电泳槽周围用冰水浸泡,以及在有游离雌激素存在的条件下进行聚焦,减少了甾体-蛋白复合物的解离。所得的结果是:孕妇血清性激素结合蛋白等电点(PI)为5.2;兔子宫内膜细胞浆雌激素受体复合物有二种组份,等电点分别为6.8及5.8。在有0.4MKCl 存在的条件下,PI6.8的放射性增加,PI5.8的放射性消失,因而前者很可能是4S 形式的甾体-受体复合物,后者很可能是8S形式的甾体-受体复合物。

Meiosis is a special type of cell division that occurs in diploid germ cells du- ring gametogenesis.To study it is of great significance in cytogenetics both theo- ratically and practically.Meiotic cells available for chromosome analysis in usu- ally prepared specimens are very scanty.The present article intends to introduce a simple method to increase the number of cells for chromosome analysis by way of testicular cell culture in vitro. Smears of cell suspension prepared by grinding mouse testicular tissue...

Meiosis is a special type of cell division that occurs in diploid germ cells du- ring gametogenesis.To study it is of great significance in cytogenetics both theo- ratically and practically.Meiotic cells available for chromosome analysis in usu- ally prepared specimens are very scanty.The present article intends to introduce a simple method to increase the number of cells for chromosome analysis by way of testicular cell culture in vitro. Smears of cell suspension prepared by grinding mouse testicular tissue were made following routine method after 4,24,48 and 72 hours culture respectively. percentages of spermatogenic cells in diakinesis and metaphase were calculated and compared with those uncultivated (control group 1) and specimens from colchicine injected mouse in vivo (control group 2).The results showed that the division index of cells from 24 hours culture was 1.16+0.52,obviously higher than that of control group 1(0.16±0.05) and a little higher than control group 2(0.42± 0.16),P<0.05.This experiment demonstrated that the mouse testicular cell may reach a number available for chromosome analysis after 4-24 hours' culture in vitro.

小鼠睾丸组织经研磨制成细胞悬浮液,按常规法分别培养4、24、48、和72小时后制片,统计出生精细胞中终变期、中期细胞所占的百分数,并与未经培养组(对照组1)及活体内注射秋水仙素后取材制片组(对照组2相比较。结果表明:经4、24小时培养的分裂指数分别为0.44±0.15、1.16±0.52,比对照组1(0.16±0.05)有明显升高;培养4小时组与对照组2(0.42±0.16)相近,但培养24小时组则高于对照组2(P<0.05)。本实验证实:小鼠睾丸细胞在体外经4—24小时的培养,也可达到增加可供染色体分析细胞数的目的。

 
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