The optimized medium composition was as follows (g/L): glucose 4, corn steep liquor 12, peptone 3, KH2PO4 2. The optimized fermentation conditions for cell growth and formation of 1-dehydrogenase were as follows: initial pH value, pH7.0; inoculum volume, 20%;
Then the conditions for HMPDD biotransformation were optimized by response surface analysis. Under optimum conditions, the conversion ratio of HMPDD was 73.3%, better than the conversion ratio before optimization.
The immobilized conditions, activation time of the immobilized cell, the concentration of K2HPO4 in the activation medium and the biotransformation conditions of immobilized cell in two-liquid-phase system were optimized.
Meanwhile, the 1 -en-dehydrogenation of 6a-methyl cortisone by Arthrobacter simplex was investigated. The transformation technique was explored and the optimization method of genetic algorithm was used to obtain the compositions of the fermentation medium. Furthermore, the dehydrogenation transformation was carried out in the 3.7L fermentor which resulted in high conversion rate.
The best conversion conditions were attained for: 7% (w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U ml-1, pH 7.8, temperature 37 °C, reaction time 40 min.
The efficient recovery of plants from avocado somatic embryos has been difficult to achieve by manipulating maturation and conversion conditions in vitro.
8 h required to achieve stationary-conversion conditions in this heterogeneous-catalysis system.
Under contact-conversion conditions, 2-azadienes-1,3 react with olefins by 1,4-cyclo-attachment to produce alkylpyridines.
We have attempted to model the rate of NO transformation on a sintered Pt-Rh/Al2O3 three-way catalyst (TWC) in various temperature and conversion conditions close to the actual ones in TWCs.
By the exchange of CMC acid and CMC Na,industrial grade CMCacid can be refined to food grade. This paper also selects the technical conditions in cxchanging process, providing the bases for industrial operation.
An industrial experiment of foamy glass preparation using fine coal ash as raw material was introduced in this paper. The optimun conversion process condition of good quality foamy glass preparation was selected during the experiment period. The application prospect of this technology in building material industry was also discussed.
A new technique of production of potassium saufate with gypsum through the two conversion steps has been studied in this paper.The conversion condition is studied and the conversion rate of gypsum reached up to 97%.