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角膜组织细胞的
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  corneal cells
     ConclusionThe decline of sFas and sFasL in aqueous humor perhaps induce the apoptosis of corneal cells,and it may be involve in the development of keratoconus.
     因为sFas和sFasL可以导致由Fas系统介导的凋亡下调,故推测房水中sFas和sFasL质量浓度的降低使角膜组织细胞的凋亡异常增加,从而导致了角膜的变薄前突,形成圆锥角膜。
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     Conclusion EGF and bFGF can stimulate the cell proliferation after the KPs with loop support implantation.
     结论 EGF和bFGF对人工角膜植入兔眼后植床角膜组织细胞的增生过程有促进作用 ,对减少人工角膜植入术后并发症 ,提高成功率有积极意义
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  相似匹配句对
     Apoptosis in cornea
     角膜细胞凋亡
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     Gene delivery to the corneal endothelium
     角膜内皮基因转移
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     In the present experiment, histochemical observations of intrinsic GUS activity in various tissues and during pollen development of tobacco (Nicotiana tabacum L.)
     不同组织细胞的内源 GUS。
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  corneal cells
Acquisition of human corneal cells for culture is hindered not only by the scarcity of donor tissues but also by some of the standard enzymatic and mechanical isolation techniques.
      
Quantification of the cytotoxic effect of UV radiation on conjunctival and corneal cells by the CASY (cell analysis) system: an
      
Growth and characterization of rabbit corneal cells in vitro
      
The indirect immunofluorescence technique represents a simple method to screen an aliquot of a cell suspension and determine the purity of corneal cells grown in vitro.
      
The corneal cells were slightly larger in patients with keratoconjunctivitis sicca.
      
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A technique for the primary culture of pure populations of rabbit corneal epithelial, keratocyte and endothelial cells is reported in this paper. Rabbit corneas were placed on concave agarose surfaces, treated with mixed solution of trypsin and EDTA. The three kinds of cells of cornea attached separately to the 24 wells culture plate surfaces, which spread out and grew to become monolayer. This method provides a simple and effective means for the reproducible initiation of primary culture of rabbit corneal epithelial,...

A technique for the primary culture of pure populations of rabbit corneal epithelial, keratocyte and endothelial cells is reported in this paper. Rabbit corneas were placed on concave agarose surfaces, treated with mixed solution of trypsin and EDTA. The three kinds of cells of cornea attached separately to the 24 wells culture plate surfaces, which spread out and grew to become monolayer. This method provides a simple and effective means for the reproducible initiation of primary culture of rabbit corneal epithelial, keratocyte and endothelial cells for use in variety of experiments.

报告了兔角膜三种细胞的体外培养技术,成功地建立了兔角膜上皮、基质和内皮细胞的原代纯培养且形成良好的细胞单层,可用于对角膜组织细胞的体外实验研究。

This paper reports the culture techniques for human corneal cells. Using this method, we have successfully established pure primary culture of three types of cells (epithelium, keratocyte, endothelium), every of which can form an adherent monolayer and become confluent. This paper also describes the morphology of each cell type in vitro. The culture of three types of cells allows their struxtur and function to be studied in vitro. The successful experience and notes are also discussed.

报告了人角膜细胞的体外培养技术,成功地建立了人角膜上皮、基质和内皮细胞的原代纯培养且形成良好的细胞单层。并对培养的三种细胞的形态进行了描述,可用于对角膜组织细胞的体外实验研究,并讨论了在该项工作中获得成功的经验和注意事项。

AbstractBy immunohistochemical staining,all 20 corneal half-buttons of HSK quiescent for 4~60 months ob-tained from 20 cases undergoing penetrating kerato-plasties were found negative of HSV-Ⅰantigen。 Theother 20 corneal half-buttons were cultured for3~4 weeks and 18 of them(90%)were found HSV-Ⅰ anti-gen positive.The 20 reactivated corneal half-buttons were co-cultured for 1 week with primary rabbit kid-ney ( RK ) cells and cellular pathological lesions ap-peared in the RK cells in 3 cultures which were also...

AbstractBy immunohistochemical staining,all 20 corneal half-buttons of HSK quiescent for 4~60 months ob-tained from 20 cases undergoing penetrating kerato-plasties were found negative of HSV-Ⅰantigen。 Theother 20 corneal half-buttons were cultured for3~4 weeks and 18 of them(90%)were found HSV-Ⅰ anti-gen positive.The 20 reactivated corneal half-buttons were co-cultured for 1 week with primary rabbit kid-ney ( RK ) cells and cellular pathological lesions ap-peared in the RK cells in 3 cultures which were also HSV-Ⅰantigen positive. These results demonstrated that the latent virus might have been harbored in thecorneal cells to escape immunohistochemical detection.In the view point of immunopathology and virology,penetrating keratoplasty can be performed On patientswith HSK quiescent over 3 months.

应用免疫组织化学染色技术,对20例稳定了4个月至5年的单疱病毒性角膜炎(HSK)的每片病变角膜片的一半行单疱病毒Ⅰ型(HSV-Ⅰ)抗原检测,结果均为阴性。另一半角膜片经组织活化培养3~4周后再做HSV-Ⅰ抗原检测,18/20(90%)为阳性。20例活化后的角膜片与RK细胞共同培养1周后,有3份RK细胞出现CPE,且HSV-Ⅰ抗原检测呈阳性。结果表明:HSK稳定期的病毒很可能潜伏到角膜组织细胞内,使抗原表达阴性而未被检出。免疫病理学和病毒学观点提示,HSK稳定3个月以上,即可考虑在需要时行穿透性角膜移植术。

 
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