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cdna杂交
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  cdna hybridization
     CLONING OF dsRNA ISOLATED FROM ROOT OF RAPHAN US SATJVUS(CV.XIU LI MEI ) AND DETECTON OF RA LATEDNESS OF dsRNA FROM DIFFERENT CULTIVARS BY cDNA HYBRIDIZATION ASSAY
     心里美萝卜dsRNA的克隆和cDNA杂交法检测不同变种萝卜dsRNA的亲缘性
短句来源
     In order to explore the relation between the CD44V gene abnormal expression and splicing and the clinicopathologic feature, the expression of CD44V of normal colorectal mucosa, adenomatous polys (precancerous disease) and colorectal carcinoma was detected using the technique of reverse transcriptase followed by amplification by PCR and cDNA hybridization.
     该研究应用RT—PCR及cDNA杂交技术,分别检测大肠正常粘膜、癌前病变及癌的CD44V mRNA表达,探讨CD44V mRNA异常表达和拼接与大肠癌临床病理之间的相关意义; 并结合免疫组化、血清学检查,对比分析大肠癌CD44V mRNA的表达与P53、PCNA、CA242、CEA对早期诊断及其在评估癌转移中的临床作用。
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  “cdna杂交”译为未确定词的双语例句
     Method:A cDNA array representing17000cDNA clusters was used to hybridize with cDNA of2normal gallbladder tissues specimens,and the average expression abundance was adopted to analyse the gene expression profile of normal gallbladder tissue.
     方法:采用含17000cDNA克隆的基因微矩阵与2例正常胆囊之cDNA杂交,取基因表达平均值,分析胆囊的基因表达谱。
短句来源
     METHODS: IL-6 was assayed using its dependent cell line MH60 . BSF2 and measured by MTT spectropho-tometry. IL-6 gene expression was proceeded by RNA isolation and hybridization with IL-6 cDNA.
     方法:用依赖株MH60·BSF2和MTT法测定IL-6,分离RNA和IL-6 cDNA杂交后测定其基因表达.
短句来源
     The length of mRNA hybridized with cDNA was detected by Northern blotting.
     并用Northern杂交实验检测与cDNA杂交的mRNA长度。
短句来源
     So the general cDNA library includes AsGV'S and AS'S, Then the library of AsGV included 1081 positive clones were screened by spot hybridization, The labeled AsGV genome DNA probe With DIG kit was cut by restriction enzyme of EcoR Ⅰ and Hind Ⅲ.
     用EcoR Ⅰ和Hind Ⅲ限制性内切酶酶切AsGV基因组DNA,制备地高辛探针,与上述总RNA、mR-NA、cDNA杂交,从文库中筛选出AsGV的阳性克隆1081个,经cDNA测序,cDNA编码序列与基因组编码序列相符。
短句来源
     Tester cDNAs were divided into two groups and ligated to the specific adaptor 1 and adaptor 2R, and then hybridized with normal aloe vera L cDNA twice with two rounds of suppression PCR.
     将实验方的双链cDNA分成两组,分别与两种不同的接头连接(adaptor 1 and adaptor 2R)。 接着与驱逐方cDNA杂交两次及两轮抑制PCR。
短句来源
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  相似匹配句对
     OPTIMIZATION OF HYBRIDIZATION EFFICIENCY IN cDNA CHIP TECHNOLOGY
     cDNA芯片技术杂交效率的优化
短句来源
     HYBRIDIZATION OF THE INSITU HIGHLY SENSIBLE TO mRNA cDNA
     mRNA-cDNA高敏感性原位分子杂交技术
短句来源
     When the cDNA was expressed in E.
     该cDNA已在E .
短句来源
     cDNA Gigapack?
     cDNA Gigapack(?)
短句来源
     e-Hybrid Rice
     e杂交
短句来源
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  cdna hybridization
Using cDNA hybridization selection techniques, we identified seven new genes in a 280 kilobase YAC coveting theHLA-F locus.
      
Assignment of human coagulation factor XII (fXII) to chromosome 5 by cDNA hybridization to DNA from somatic cell hybrids
      
Here, we describe three new genes isolated from the 3p YAC contig by using a cDNA hybridization selection.
      
According to cDNA hybridization, the three genes are expressed in a tissue-specific manner.
      
The genomic surroundings of this gene have been characterized together with the sequences of two strongly conservedGpa pseudogenes isolated from a genomic maize library by differential cDNA hybridization.
      
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Globin mRNAs have been extraeted from the reticulooytes of the rabbit and chicken, and their translation activities were measured in the wheat embryo cell-free system. In comparison with the control, rabbit and chicken globin mRNA, respectively, gave a 32-fold and 10-fold stimulation of ~(14)C-leucine incorporation. The translation products of these mRNAs co-migrated with authentic globin upon electrophoresis on SDS-polyacrylamide gel. In addition, single-and double-stranded globin eDNA and the mRNA-cDNA hybrid...

Globin mRNAs have been extraeted from the reticulooytes of the rabbit and chicken, and their translation activities were measured in the wheat embryo cell-free system. In comparison with the control, rabbit and chicken globin mRNA, respectively, gave a 32-fold and 10-fold stimulation of ~(14)C-leucine incorporation. The translation products of these mRNAs co-migrated with authentic globin upon electrophoresis on SDS-polyacrylamide gel. In addition, single-and double-stranded globin eDNA and the mRNA-cDNA hybrid strand have been synthesized from the mRNAs mentioned above with AMV reverse transeriptase and DNA polymerase 1. Analyzed by gel-electrophoresis the lengths of these eDNA fragments were 500y~700, 550~800 and 500~800 bp, respectively, and in the ds-cDNA synthesized, fragments with 550~800 bp represent about 30% of the total cDNA.

本文报道从人工贫血的兔与鸡的网织红细胞中提取珠蛋白mRNA,在麦胚无细胞体系中测定其蛋白翻译活力,与对照相比,兔、鸡珠蛋白mRNA促进~(14)C-亮氨酸参入新生蛋白质的活力分别达到32倍和10倍,所翻译的蛋白产物在聚丙烯酰胺凝胶上的行为与天然珠蛋白一致。上述mRNA在AMV反转录酶和DNA聚合酶的作用下,分别合成了单链和双链的cDNA及mRNA-cDNA杂交链,凝胶电泳分析其长度分别为500~700,550~800及500~800碱基对,550~800碱基对大小的双链cDNA约占总合成量的30%。

Globin mRNAs have been extracted from the reticulocytes of the rabbit andchicken, and their translation activities were measured in the wheat embryo cell-freesystem. In comparison with the control, rabbit and chicken globin mRNA, respec-tively, gave a 32-fold and 10-fold stimulation of ~(14)C-leucine incorporation. The tran-slation products of these mRNAs co-migrated with authentic globin upon electropho-resis on SDS-polyacrylamide gel. In addition, single-and double-stranded globincDNA and the mRNA-cDNA hybrid...

Globin mRNAs have been extracted from the reticulocytes of the rabbit andchicken, and their translation activities were measured in the wheat embryo cell-freesystem. In comparison with the control, rabbit and chicken globin mRNA, respec-tively, gave a 32-fold and 10-fold stimulation of ~(14)C-leucine incorporation. The tran-slation products of these mRNAs co-migrated with authentic globin upon electropho-resis on SDS-polyacrylamide gel. In addition, single-and double-stranded globincDNA and the mRNA-cDNA hybrid strand have been synthesized from the mRNAsmentioned above with AMV reverse transcriptase and DNA polymerase 1. Analyzedby gel-electrophoresis the lengths of these cDNA fragments were 500~700, 550~800and 500~800 bp, respectively, and in the ds-cDNA synthesized, fragments with550~800 bp represent about 30% of the total cDNA.

本文报道从人工贫血的兔与鸡的网织红细胞中提取珠蛋白mRNA,在麦胚无细胞体系中测定其蛋白翻译活力,与对照相比,兔、鸡珠蛋白mRNA促进~(14)C-亮氨酸参入新生蛋白质的活力分别达到32倍和10倍,所翻译的蛋白产物在聚丙烯酰胺凝胶上的行为与天然珠蛋白一致。上述mRNA在AMV反转录酶和DNA聚合酶的作用下,分别合成了单链和双链的cDNA及mRNA-cDNA杂交链,凝胶电泳分析其长度分别为500~700,550~800及500~800碱基对,550~800碱基对大小的双链cDNA约占总合成量的30%。

Expression of the C-myc oncogene is tightly regulated in a normal cell, but is often, although not always, enhanced in some types of tnmor cells. We have previously suggested that a normal cell is equipped with a secure mechanism, possibly a cis-acting gene-to-gene interaction, ensuring that constitutive or excessive C-myc activity does not occur. In the present experiment, we examined this possibility using a mouse genomic C-myc clone. We present evidence that a novel transcription unit ( "gene" ) , presumably...

Expression of the C-myc oncogene is tightly regulated in a normal cell, but is often, although not always, enhanced in some types of tnmor cells. We have previously suggested that a normal cell is equipped with a secure mechanism, possibly a cis-acting gene-to-gene interaction, ensuring that constitutive or excessive C-myc activity does not occur. In the present experiment, we examined this possibility using a mouse genomic C-myc clone. We present evidence that a novel transcription unit ( "gene" ) , presumably 15-30 kb long, lies 2.4-3.4 kb upstream from C-myc in the same transcriptional orientation and actively produces a single species of 5.8 kb transcript in normal cells where C-myc RNA is hardly detected. The observed intergenic distance between the C-myc and tne neighbouring "gene" is much shorter than the length defined as necessary for a pair of simultaneously active genes.Consequently, C-myc is thought to be under transcriptional interference from the neighbouring "gene" .

本文利用cDNA探针,以Southern杂交在C-myc 5′上游区域发现了一段活跃转录的序列(文中称“旁侧基因”)。对该基因克隆及进一步分析表明,能与cDNA杂交的序列存在于距C-myc 5′端约2.4kb的Sma Ⅰ片段中。Northern杂交显示,该旁侧基因在不同组织均有表达,产物为5.8kb,推断该基因为15~3.5kb。以旁侧基因部分序列为探针,在不同种属的基因组中检测出单拷贝序列,提示该基因在进化上的保守性。RNase Mapping分析发现此旁侧基因与C-myc转录方向相同,转录终止在C-myc5’上游约2.4~3.4kb区域。根据基因领域效应及本文结果,我们推测,在正常细胞中,由于旁侧基因的领域效应,使C-myc保持相对静止状态,而在肿瘤细胞中,染色体转位或原病毒插入,破坏了旁侧基因的领域效应,使C-myc表达增高。

 
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