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蛋白杂交
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  protein hybridization
     Correlation between neck lymph node metastasis (NLNM) and αcatenin,βcatenin expression in oral squamous cell carcinoma (OSCC) was studied through samples from 68 cases by protein hybridization adopting Western blotting to detect the expression level of αcatenin and βcatenin. As compared with OSCC without NLNM (32 cases), the expression level of αcatenin and βcatenin in OSCC with NLNM (36 cases) lowered apparently (P<0.01).
     采用蛋白杂交技术 ,对 6 8例口腔癌中的α catenin和 β catenin蛋白表达进行了研究 ,探讨其表达与口腔癌颈淋巴结转移的相关性 ,发现与未发生淋巴结转移的口腔鳞癌相比 (32 ) ,淋巴结转移的口腔鳞癌组织中(36 ) ,α catenin和 β catenin蛋白表达出现明显的降低 (P <0 .0 1 )。
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     The result of t test was P <0.01.By the results of protein hybridization scan, peak area in selenium adequate group was 3 438 and in selenium deficient group was 2 067.Conclusion Selenium deficiency decreases the activity and protein levels of thioredoxin reductase in cardiomyocytes,and decreases cardiomyocytes anti oxidation and induce cardiomyocytes injury.
     蛋白杂交扫描结果显示 ,常硒组峰面积为 3438,低硒组峰面积为 2 0 6 7。 结论 低硒降低硫氧还蛋白还原酶的活性和蛋白表达水平 ,使心肌抗氧化能力减低 ,引起心肌损伤。
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  “蛋白杂交”译为未确定词的双语例句
     ② To study the effect of PARP1 binding with rTEF1a on their specific DNA binding abilities and the influence of poly(ADP)-ribosylation on the DNA binding ability of PARP1-rTEF1a complex, Southern-Slot ( protein-DNA ) blot method was used.
     使用狭缝DNA 蛋白杂交技术研究PARP1与rTEF1a结合后对它们各自特异性DNA结合能力的影响 ,以及多聚ADP核糖化反应对PARP1 rTEF1a复合物DNA结合能力的影响。
短句来源
     The expression ratio of NKX3.1 protein in the epithelia cells of prostate was 100%,but in testis mammary gland was(16.7)%,in bladder and intestine was(8.3)%,and in kidney,liver,fat and skin was 0%(P<(0.01)).
     蛋白杂交显示NKX 3.1蛋白总阳性表达率前列腺组织为100%,睾丸、乳腺组织均为16.7%,膀胱、回肠组织为8.3%,其他肾脏、肝脏、脂肪和皮肤组织均为0(P<0.01)。
短句来源
     [Methods]76 prostate tissues(32 cancer, 12 normal prostate and 32 benign prostate hyperplasia tissues) and 96 non-prostate tissues were analysed for the expression of NKX3.1 mRNA and protein by using semi-quantitative RT-PCR.
     采用半定量RT-PCR、蛋白杂交和免疫组化检测76例前列腺组织(32例前列腺癌、32例前列腺增生、12正常前列腺)和96例非前列腺组织标本NKX3.1mRNA、NKX3.1浙江大学外科学(泌尿外科)博士学拉论文--娜开究摘要}}肠已胜.
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     Western blotting was used to detect the expression of HNF4 in nuclear matrix proteins.
     蛋白杂交(Western)分析HNF4的表达。
短句来源
     Methods:Aortic arterioles smooth muscle cells were isolated and cultured, and the protein and mRNA of type I IP 3 R and report gene in aortic arterioles smooth muscle cells treated with IL-1 were detected by Western blot, Northern blot and Dual-luciferase reporter assay system.
     方法 :通过对主动脉平滑肌细胞培养 ,应用蛋白杂交技术、核酸杂交技术分别检测在IL 1β作用后 ,主动脉平滑肌细胞中Ⅰ型三磷酸肌醇 (IP3)受体蛋白和Ⅰ型IP3受体mRNA的表达及Ⅰ型IP3受体mRNA半衰期的测定 ,同时研究IL 1β对Ⅰ型IP3受体基因启动子的作用。
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  相似匹配句对
     The Analysis of Hybrid Rice Seed Protein with Polyacrylamide Gel Electrophoresis
     杂交水稻种子蛋白电泳分析
短句来源
     Southwestern blot hybridization of DN A-binding proteins
     DNA结合蛋白的Southwestern印迹杂交
短句来源
     e-Hybrid Rice
     e杂交
短句来源
     proteinuria(-);
     尿蛋白(-);
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     northern hybridization.
     Northern杂交
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  protein hybridization
All sample processing and protein hybridization were performed at the same time to negate any potential technical variability.
      
Nev ertheless, very little evidence of such protein hybridization has been found among the ethanol seed extracts used in these studies.
      


A method of Southwestern blot hybridization to detect DNA-binding proteins is reported,Nuclearproteins were extracted and separated by SDS-PAGE, then transfered onto two pieces of nitrocellulose filters.(32) ̄Plabelled DNA probes were used to hybridize with the proteins on the fiIters. The DNA-binding proteins could bedetected with autoradiograph, and their molecular weights could be measured by comparing with marker protein.

报道了一种检测DNA结合会白的Somhwestem印迹杂交方法。将核蛋白粗提物进行SDS-PAGE,然后通过扩散法转移至两张硝酸纤维素膜上,再用(32) ̄P标记的DNA探针与膜上蛋白杂交,根据放射自显影图谱可知DNA结合蛋白在膜上的分布,与标准分子量大小的蛋白电泳谱相比较,可测出这些蛋白的分子量。

Two hybridoma cell lines secreting McAbs: against HPV16 virus-like particles, which comprises eukaryotic expression system derived L1/L2 capsid proteins, were established. The antibodiesthey secreted only react with HPV16 virus-like Particles while have no reaction with HPV16 E6/E7protein, human serum albumin or bovine serum albumin.It was proved immunohistochemically thatthese antibodies could be sensitively and specifically used in clinical detection of HPV16 infection.

用真核表达的HPV16L1/L2蛋白作为抗原,经免疫、融合、选择性培养、克隆化等过程,我们建立了两株抗HPV16L1/L2蛋白的杂交瘤细胞株(另有数株仍在建株中)。免疫斑点法检测证明,它们产生的抗体,只与HPV16L1/L2蛋白起反应,而不与HPV16E6、E7蛋白、人血清蛋白和牛血清蛋白起反应。另外,免疫组化试验证明,这种抗体可应用于临床HPV16感染的检验,具有敏感、特异、准确、快捷、经济的优点。

Hybridomas secreting McAbs to human collagen type Ⅳ were established by immunization of BALB/c mice with human collagen type Ⅳ (Ⅳ-CL). Five of these McAbs A2, A4,A5, B3 and E2 were well characterized and the Ig class and subclass were determined as IgG1,IgG2, IgG2b, IgG2 and IgG1 respectively. These McAbs reacted strongly with Ⅳ-CL moleculeswith the affinity constants(Kaff) of 6.6×108M-1~6.0×1010M-1 and did not have cross-reactivity with other types of collagen and irrelevant proteins.

用人Ⅳ型胶原蛋白免疫BALB/c小鼠,克隆了一组分泌抗人Ⅳ型胶原蛋白的杂交瘤细胞株,并且对其中的A2、A4、A5、B3和E2进行了鉴定。其抗体亚类分别为IgG1、IgG2、IgG2b、IgG2和IgG1。其亲和力常数Kaff=6.6×108M(-1)~6.0×10(10)M(-1)。它们均能特异性地识别人Ⅳ型胶原蛋白。

 
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