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   山羊fsh 的翻译结果: 查询用时:0.28秒
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山羊fsh
相关语句
  goat fsh
     The mature peptide had a predicted molecular weight of 12.53kDa and two potential AMinked glycosylation sites at residues 7 and 24. The amino acid sequence of the goat FSH a-subunit was 100%, 97.5% and 98.3% identical to that of sheep, cattle and water buffalo, while the goat FSH P-subunit was 97.7%, 93.1% and 93.1 % identical to that of sheep, cattle and swine.
     山羊FSHα亚单位与已发表的绵羊、牛、水牛的氨基酸序列同源性分别为100%、97.5%和98.3%,山羊FSH β亚单位与已发表的绵羊、牛和猪的氨基酸序列同源性分别为97.7%、93.1%和93.1%。
短句来源
     Those vectors were expressed in COS-7 cells or CHO cells respectively. Moreover, CHO cells expressed goat FSH stably were acquired.
     在COS-7和CHO细胞中得到了表达,并获得了稳定表达山羊FSH的CHO细胞。
短句来源
     These results all gained above not only discover molecular structure of goat FSH, but also lay the groundwork for the research of genetic engineering product, immunity and application of goat FSH.
     本实验研究结果,不仅揭示了山羊FSH的分子结构,而且为山羊FSH基因工程产品的研制,及开展山羊FSH免疫与应用研究奠定了基础。
短句来源
     After sequencing the vector was transfected into CHO cells and stable expressing cells were selected by hygromycin B. These results indicated that the long-acting goat FSH gene was acquired and the expressed products of stable expressing CHO cell is 0.105 mIU/mL. This work is important for future research on long-acting mechanism of CTP.
     结果表明获得了长效山羊FSH基因,并得到稳定表达的CHO细胞株,表达量为0.105mU/mL,为进一步研究CTP结构与功能的关系,以及长效激素的理化特性奠定了基础。
短句来源
     The single strand gFSHβ-CTP-α gene was successfully constructed by the combination of the C-terminal peptide(CTP) of carboxyl-terminal region of human chorionic gonadotropin with the goat FSH α-subunit and β-subunit genes. The gene was subcloned into expression vector pVITRO and transfected into Chinese Hamster Ovary(CHO) cells after gene sequencing.
     以HCGβ亚基羧基末端延长肽CTP基因为连接序列,重组山羊FSHα和β亚基基因,获得单链gFSHβ-CTP-α,克隆入表达载体pVITRO,测序后转染CHO细胞。
短句来源
  “山羊fsh”译为未确定词的双语例句
     In the complete 641 base pairs of FSH β gene in-1,the contents of A,C,G and T were 35.73%,(13.88)%,17.47% and 32.92%,respectively,the content of A+T(68.65%) was higher than that of G+C(31.35%).
     山羊FSHβ基因内含子1全序列长641bp,A、C、G、T 4种碱基分别占35.73%,13.88%,17.47%,32.92%; A+T(68.65%)的含量远高于G+C(31.35%)的含量。
短句来源
     In the complete 641 base pairs of FSHβgene in-1,the content of A,C,G,T was 35.73%,13.88%,17.47% and 32.92%,respectively. The content of it was significantly that the A+T (68.65%) was higher than the content of G+C (31.35%).
     山羊FSHβ基因内含子1全序列长641 bp,A、C、G、T4种碱基分别占35.73%、13.88%、17.47%、32.92%,A+T (68.65%)的含量远高于G+C(31.35%)的含量。
短句来源
     Construction and Stable Expression of Eukaryotic Expression Vector pVITRO-FSHαβ of Goat Follicle-stimulating Hormone in Chinese Hamster Ovary Cells(CHO)
     山羊FSH真核表达载体pVITRO-FSHαβ构建及在CHO细胞内稳定表达
短句来源
     Studies on Polymorphisms Analysis in Exon10 of FSH Receptor Gene and Their Relationship with Reproduction Trait in Goats
     山羊FSH受体基因第10外显子多态性及其与繁殖性状关系的研究
短句来源
     7,The results of superovulation of useing FSH+PG is better than CIDR+FSH for the goat in Yaan.
     7.对雅安土种山羊,FSH+PG法好于CIDR+FSH法的超排效果。
短句来源
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  相似匹配句对
     Construction and Expression of Goat Follicle-stimulating Hormone Analogous Gene
     山羊FSH类似物基因构建及其表达
短句来源
     The Wolf and the Goat
     狼和山羊
短句来源
     Electrocardiographic Patterns of Goats
     山羊心电图
短句来源
     EFFECT FSH ON GOAT FOLLICULAR POPULATION DEVELOPMENT
     促卵泡素(FSH)对山羊卵巢卵泡群生长发育的影响
短句来源
     No changes of the concentration of FSH were observed.
     FSH无明显改变。
短句来源
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To find single nucleotide polymorphism (SNP) mutant of goat follicle-stimulating hormone receptor(FSHR) gene loci,to investigate polymorphism of FSHR gene and to explore possibility of this gene function in goat polyembryong,the 5'-flanking region of FSHR was amplified by PCR using genomic DNA of Xiangdong black goats as the template and designing primers according to the sequence of the sheep receptor gene.The sequence was determined and analyzed according to cloning,and the sequence was compared with that...

To find single nucleotide polymorphism (SNP) mutant of goat follicle-stimulating hormone receptor(FSHR) gene loci,to investigate polymorphism of FSHR gene and to explore possibility of this gene function in goat polyembryong,the 5'-flanking region of FSHR was amplified by PCR using genomic DNA of Xiangdong black goats as the template and designing primers according to the sequence of the sheep receptor gene.The sequence was determined and analyzed according to cloning,and the sequence was compared with that of bovine and sheep FSHR gene.The results indicated that the amplified PCR fragment was 844 bp,which had significant similarity (94.08%) to that in Chinese Simmental bovine;There were 5 base insertions in the left-flanking of -364 bp,7 base deletions at -625 bp~-619 bp.While compared with the FSHR gene of Australian sheep,the homology was 93.60%,there were 2 base insertions in the left-flanking of -367 bp and only 1 base deletion respectively at-630 bp,-145 bp and -97 bp of the sequence in Xiangdong black goats.

为找寻山羊FSH受体基因的SNP突变位点,进行FSH受体基因的多态性分析和探索该受体基因在山羊多胎中的可能作用,利用比较基因组学的方法,根据绵羊FSH受体基因序列设计引物,以湖南湘东黑山羊基因组为模板,PCR扩增了FSH受体基因5' 端侧翼调控区,通过克隆进行了序列测定及分析,并与牛、绵羊该区段序列进行了比较.结果表明,PCR扩增获得的片段为844 bp,与中国西门塔尔牛该段序列相比,同源性为94.08%,在-364 bp处的左翼发生了多碱基插入,湘东黑山羊该序列有5个碱基的插入,另在-625~-619 bp处的7个碱基缺失;与澳洲绵羊的FSHR基因比较,同源性为93.60%,在-367 bp处的左翼,湘东黑山羊该段序列有2个碱基的插入,在-630,-145,-97 bp 处各有1个碱基缺失.

Design of a long-acting goat follicle-stimulating hormoneFSH agonist gene by fusing the C-terminal sequence of the chorionic gonadotropin β subunit to the goat FSH β subunit. This gene was cloned into dual eukaryotic expression vector pVITRO. After sequencing the vector was transfected into CHO cells and stable expressing cells were selected by hygromycin B. These results indicated that the long-acting goat FSH gene was acquired and the expressed products of stable expressing CHO cell is 0.105 mIU/mL. This...

Design of a long-acting goat follicle-stimulating hormoneFSH agonist gene by fusing the C-terminal sequence of the chorionic gonadotropin β subunit to the goat FSH β subunit. This gene was cloned into dual eukaryotic expression vector pVITRO. After sequencing the vector was transfected into CHO cells and stable expressing cells were selected by hygromycin B. These results indicated that the long-acting goat FSH gene was acquired and the expressed products of stable expressing CHO cell is 0.105 mIU/mL. This work is important for future research on long-acting mechanism of CTP.

通过PCR重叠延伸法将人绒毛膜促性腺激素(hCG)的羧基末端延长肽(CTP)基因连接至山羊促卵泡素(FSH)β亚基的羧基末端,克隆入双表达载体pVITRO,测序证实后将表达载体转染CHO细胞,用潮霉素B筛选稳定表达的CHO细胞株。结果表明获得了长效山羊FSH基因,并得到稳定表达的CHO细胞株,表达量为0.105mU/mL,为进一步研究CTP结构与功能的关系,以及长效激素的理化特性奠定了基础。

According to the FSH β gene sequence of bovine and sheep,the primers were designed and follicular-stimulating hormone(FSH) β gene in-1 from Nanjiang Huang goat was sequenced.In the complete 641 base pairs of FSH β gene in-1,the contents of A,C,G and T were 35.73%,(13.88)%,17.47% and 32.92%,respectively,the content of A+T(68.65%) was higher than that of G+C(31.35%).The gene sequences from seven individuals were completely similar,which indicated that FSH β gene in-1 was very conservative.

利用牛、绵羊等动物FSHβ亚基基因序列设计引物,采用PCR法扩增并测定出南江黄羊FSHβ亚基基因内含子1全序列(GenBank登录号:AY838283)。山羊FSHβ基因内含子1全序列长641bp,A、C、G、T 4种碱基分别占35.73%,13.88%,17.47%,32.92%;A+T(68.65%)的含量远高于G+C(31.35%)的含量。所测定7个个体的碱基序列完全一致,表明FSHβ基因内含子1的保守性较强。

 
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