助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   前列腺癌细胞pc3 的翻译结果: 查询用时:0.01秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

前列腺癌细胞pc
相关语句
  prostate cancer cell pc 3
     Inhibitory effect of photoactivated hypericin on growth of human prostate cancer cell PC3M
     光活化的金丝桃素对人前列腺癌细胞PC3M的生长抑制作用
短句来源
     Inhibitory effect of DIM on growth of human prostate cancer cell PC3M
     DIM对人激素非依赖性前列腺癌细胞PC3M的生长抑制作用
短句来源
     Objective To study the effect of pSilencerl. 0-U6-siRNA-STAT3 on the growth of tumor transplanted from human prostate cancer cell PC3 into nude mice. Methods The PC3 cells were transplanted into nude mice,then the time of tumor formation and growth rates were observed.
     目的探讨pSilencerl.0-U6-siRNA-STAT3重组质粒对人前列腺癌细胞PC3裸鼠移植瘤生长的抑制作用。
短句来源
  “前列腺癌细胞pc3”译为未确定词的双语例句
     Methods Under 1 G, MC3T3-E1 cells were cocultured with prostate cancer cell PC-3 for 48 hours. Then the proliferation and ALP activity of MC3T3-E1 were determined by MTT and PNPP method respectively.
     方法 1 G条件下,将小鼠成骨样细胞MC3T3 E1与人前列腺癌细胞PC 3分别接种在混合培养板的外室和内室中培养48 h, 用MTT法检测MC3T3 E1的增殖、用PNPP法检测MC3T3 E1 的碱性磷酸酶(ALP)活性。
短句来源
     The effect of NF κB on the cytotoxicity of TNF on L929 and prostate cancer cells PC 3 in vitro was studied.
     培养人前列腺癌细胞PC 3和成纤维细胞L92 9,体外分析NF κB抑制剂对TNF细胞毒作用的影响。
短句来源
     Methods Cell counts and MTT method were used to detect the influence of ATP on the growth of 1E8 (metastatic) and 2B4 (non metastatic) cells derived from human prostate cancer cell line PC3M.
     方法 用细胞计数及MTT法检测外源性P2嘌呤受体激动剂ATP对人前列腺癌细胞PC 3M亚系 1E8(高转移 )和 2B4(低转移 )体外生长的影响。
短句来源
     Objective To study the inhibitory effect of(3,3)-Diindolylmethane(DIM) on PC3M cells.
     目的:探讨3,3-二吲哚基甲烷(3,3-Diindolylmethane,DIM)对人激素非依赖性前列腺癌细胞PC3M的生长抑制作用。
短句来源
     Highest inhibiting activity was achieved when R=2,4-dimethoxy (5m), with an inhibition rate of 92.0% at the concentration of 5 μM. Compound 51 (R=3, 5-dichloro) could inhibit PC3 cells proliferation of 93.9% at the concentration of 5 uM.
     另外,采用MTT法进行了新化合物抑制PC3、Bcap37和BGC823癌细胞体外试验,结果表明:在5μmol/L浓度下,化合物5I(R=3,5-二氯)、5m(R=2,4-二甲氧基)对人体前列腺癌细胞PC3的抑制率分别为93.9%和92.0%。
短句来源
更多       
  相似匹配句对
     Effects of zinc on apoptosis of prostate cancer cells
     锌对前列腺癌细胞PC-3M凋亡的影响
短句来源
     Effects of Flavonoid on the Proliferation of Prostate Cancer Cells PC-3
     异黄酮类化合物对前列腺癌细胞PC-3增殖的抑制
短句来源
     4. Positive staining of ET-1 * ETAR and IGF- I R was found in PC-3Mcells.
     4.前列腺癌细胞PC3M细胞中 ET-l、ET。
短句来源
     Doxycycline inhibits the proliferation of PC- 3 cells in vitro.
     强力霉素抑制前列腺癌细胞PC-3增殖的体外研究
短句来源
     VP16 INDUCED APOPTOSIS IN PC3 CELLS
     VP-16诱导前列腺癌细胞PC-3凋亡的研究
短句来源
查询“前列腺癌细胞pc3”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


In order to study the efficiency of the antibody directed enzyme prodrug therapy (ADEPT),the effect of MTX α peptides on growth and apoptosis of the prostatic cancer cells was studied.Methotrexate a Phenylalanine (MTX α Phe) and Methotrexate a arginine (MTX α Arg) were used as prodrugs synthesised by solid phase methods.The cytotoxin and cell cycle of prostatic cancer cells,PC 3 and PC 3m were determined.MTX α Phe was hydrolyzed into MTX by carboxypeptidase (CPA) using HPLC and Mass Spectrograph.The...

In order to study the efficiency of the antibody directed enzyme prodrug therapy (ADEPT),the effect of MTX α peptides on growth and apoptosis of the prostatic cancer cells was studied.Methotrexate a Phenylalanine (MTX α Phe) and Methotrexate a arginine (MTX α Arg) were used as prodrugs synthesised by solid phase methods.The cytotoxin and cell cycle of prostatic cancer cells,PC 3 and PC 3m were determined.MTX α Phe was hydrolyzed into MTX by carboxypeptidase (CPA) using HPLC and Mass Spectrograph.The cytotoxin effect of the products enzymed by CPA was 100 times higher than that of the prodrugs with no obvious cytotoxin on the cells in vitro and former showed an obvious inhibition effects on the cells.Their cell cycle was determined and S phase fraction was lower than the control group,G 0/G 1 fraction was higher and the perliferation index obviously decreased resulting in apoptosis of the cancer cells.MTX α Phe would be considered an excellent prodrug.

研究前列腺癌的导向酶前体药物疗法(ADEPT),在体外观察前体药物甲氨喋呤α肽对前列腺癌细胞生长、凋亡的影响。用固相合成法合成了前体药物甲氨喋呤α苯丙氨酸(MTXαPhe)和甲氨喋呤α精氨酸(MTXαArg),并对前列腺癌细胞PC3、PC3m进行了细胞毒性及周期动力学分析。用高效液相色谱及质谱分析,MTXαPhe可被羧肽酶A(CPA)水解并且完全释放出MTX。经体外对前列腺癌细胞的毒性试验,前体药物基本上对细胞无毒,经CPA水解后其活性MTX细胞毒性大于前体药物100倍以上。对前列腺癌细胞具有显著抑制作用,S期比率明显低于对照组,G0/G1期比率高于对照组,细胞增殖指数明显减低,细胞并发生凋亡。认为MTXαPhe是一较理想的前体药物。

AIM: To study the efficiency 0f the antibody di-rected enzyme-pr0drug therapy(ADEPT),the effect 0f MTX-a-peptides on the pr0state cancer was studied in vivo and invitro. METHODS: The cell-toxic effect and cell cycle analysisof Methotrexate -a- Phenylalanine (MTX-α-Phe ) andMethotrexate-α-Arginine (MTX-α-Arg ) on the cells 0fprostate cancer, PC-3 and PC-3m were determined. Theinhibition of the growth 0f the tumor of the former drugs innude mouse was observed. RESULTS: The prodrugs almosthad not the toxic effect...

AIM: To study the efficiency 0f the antibody di-rected enzyme-pr0drug therapy(ADEPT),the effect 0f MTX-a-peptides on the pr0state cancer was studied in vivo and invitro. METHODS: The cell-toxic effect and cell cycle analysisof Methotrexate -a- Phenylalanine (MTX-α-Phe ) andMethotrexate-α-Arginine (MTX-α-Arg ) on the cells 0fprostate cancer, PC-3 and PC-3m were determined. Theinhibition of the growth 0f the tumor of the former drugs innude mouse was observed. RESULTS: The prodrugs almosthad not the toxic effect on the test cell in vitro. MTX-α-Phewas hydrolyzed into MTX by Carboxypeptidase (CP-A). Thetoxin effect of the products was about 1OOO times higher thanthat of the prodrugs and the former showed an obvious inhibi-tion effects on the prostate cancer cells. Their cell-cycle wasdetermined and showed that the growth of the cell was inhib-ited in the S-phase. The inhibition on the tumor growth ofprostate cancer of the animal model was obviously in the ther-apy of the ADEPT with the A-MTX-α-Phe combined with theCP-A. The living condition was better than other groups.CONCLUSION: The MTX-α-Phe and the CP-A was an idealADEPT.

目的:研究前列腺癌的导向酶-前体药物疗法(ADEPT),在体内外观察前体药物甲氨喋呤-α-肽对前列腺癌的作用.方法:用前体药物甲氨喋呤-α-苯丙氨酸(MTX-α-Phe)和甲氨喋呤-α-精氨酸(MTX-α-Arg)对前列腺癌细胞PC-3,PC-3m进行了细胞毒性及细胞动力学分析,并对前列腺癌荷瘤裸鼠模型行肿瘤生长抑制的观察.结果:体外细胞毒性试验,前体药物对任何细胞均无毒,但MTX-α-Phe经羧肽酶-A(CP-A)水解后,其细胞毒性大于前体药物约1000倍,对前列腺癌细胞有显著抑制作用,细胞动力学分析肿瘤细胞生长明显受阻于S期.动物模型体内试验,经抗人精浆蛋白单克隆抗体导向的羧肽酶-A-MTX-α-PheADEPT治疗组,肿瘤生长明显抑制,动物生活质量优于单用MTX组.结论:MTX-α-Phe+CP-A是一理想的前列腺癌的ADEPT对.

Human prostate secretory protein of 94 amino acids (PSP94) cDNA including signal and mature peptide has been successfully obtained by reverse transcription polymerase chain reaction (RT PCR) from human hypertrophic prostate mRNA.Sequence analysis indicated that the sequence of human PSP94 cDNA was the same as that of human natural PSP cDNA.The hPSP94 cDNA and human TNFα mutant (TNF Δ) gene were fused by an artificial linker SAPGTP.The chimeric gene coding for 5′PSP SAPGTP TNF Δ fusion protein under control...

Human prostate secretory protein of 94 amino acids (PSP94) cDNA including signal and mature peptide has been successfully obtained by reverse transcription polymerase chain reaction (RT PCR) from human hypertrophic prostate mRNA.Sequence analysis indicated that the sequence of human PSP94 cDNA was the same as that of human natural PSP cDNA.The hPSP94 cDNA and human TNFα mutant (TNF Δ) gene were fused by an artificial linker SAPGTP.The chimeric gene coding for 5′PSP SAPGTP TNF Δ fusion protein under control of the phage P RP L promoter was constructed.The expression was assayed by SDS PAGE,which showed that the amount of the expressed the protein was about 35% of total bacterial proteins.Western blot indicated that the molecular weight of 5′PSP SAPGTP TNF Δ was in accordance with the estimated value of 31 kD.Through biological activity analysis,the fusion protein has cytotoxicity activity of L929 and PC 3 cell.

利用RT-PCR从人肥大前列腺组织钓取94个氨基酸的人前列腺分泌蛋白(PSP94)全长cDNA,序列分析结果与文献报道的完全一致.将PSP94成熟肽与人TNFα衍生物(TNFΔ)通过Linker-SAPGTP在基因水平上融合成5′PSP94-TNFΔ,融合基因DNA序列分析结果与设计的相符合.5′PSP94-TNFΔ在大肠杆菌中表达产物分子量约为31kD,表达量约占菌体总蛋白量的35%.以L929细胞和人前列腺癌细胞株PC-3为靶细胞进行细胞毒分析结果表明,5′PSP94-TNFΔ融合蛋白既具有TNF的细胞毒活性,又具有对前列腺癌细胞PC-3的杀伤作用

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关前列腺癌细胞pc3的内容
在知识搜索中查有关前列腺癌细胞pc3的内容
在数字搜索中查有关前列腺癌细胞pc3的内容
在概念知识元中查有关前列腺癌细胞pc3的内容
在学术趋势中查有关前列腺癌细胞pc3的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社