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重症戊型肝炎
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  severe hepatitis e
     Methods Umbilical cord blood of 18 neonates of 17 pregnant women with severe hepatitis E and their mothers′ blood were examined for anti HEV IgM/IgG by using ELISA, were studial with metbod liver biopsy specimens of the neonates, HEV antigens (HEV Ag) in the biopsy specimens, were detected by using immunohistochemical method and HEA RNA was detected by using in situ hybridization with DIG probe labelled with HEVcDNA .
     方法对17例患重症戊型肝炎的孕妇和娩出的18例新生儿,以酶联免疫吸附法测母血和脐带血抗-HEVIgM/IgG,同时查新生儿肝组织病理改变,免疫组化法查肝内戊型肝炎病毒抗原(HEVAg),用地戈辛(DIG)探针标记HEVcDNA23qbp片段进行原位杂交查肝内HEVRNA。
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  相似匹配句对
     The characteristics in elderly hepatitis E patienrs
     老年肝炎的特点
短句来源
     Studies on etiology and vaccines of hepatitis E.
     重视肝炎研究
短句来源
     Studies on hepatitis E in China
     我国肝炎研究
短句来源
     Case-control Study of Risk Factors on Epidemic of Hepatitis E
     肝炎流行危险因素的研究
短句来源
     EPIDEMIOLOGICAL STUDY ON VIRAL HEPATITIS IN HUNAN PROVINCE
     湖南省肝炎血清流行病学调查
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Objective To clarify causes for intrauterine infection of hepatitis E virus (HEV) and deaths of fetuses and neonates. Methods Umbilical cord blood of 18 neonates of 17 pregnant women with severe hepatitis E and their mothers′ blood were examined for anti HEV IgM/IgG by using ELISA, were studial with metbod liver biopsy specimens of the neonates, HEV antigens (HEV Ag) in the biopsy specimens, were detected by using immunohistochemical method and HEA RNA was detected by using in situ hybridization with...

Objective To clarify causes for intrauterine infection of hepatitis E virus (HEV) and deaths of fetuses and neonates. Methods Umbilical cord blood of 18 neonates of 17 pregnant women with severe hepatitis E and their mothers′ blood were examined for anti HEV IgM/IgG by using ELISA, were studial with metbod liver biopsy specimens of the neonates, HEV antigens (HEV Ag) in the biopsy specimens, were detected by using immunohistochemical method and HEA RNA was detected by using in situ hybridization with DIG probe labelled with HEVcDNA .Results The pregnant women were positive for both anti HEV IgM/IgG in venous blood and anti HEV IgG was positive in the cord blood specimens. The histopathological examinations showed degenerations and inflammatory cells infiltration The positive rate of immunohistochemistry for HEVAg was 77% (10/13) and positive rate of in situ hybridization for HEV RNA was 62% (8/13). Conclusion These results indicated that of intrauterine infection with HEV does exist.

目的为了从分子水平探讨孕妇感染戊型肝炎病毒(HEV)后胎儿宫内感染和死亡原因。方法对17例患重症戊型肝炎的孕妇和娩出的18例新生儿,以酶联免疫吸附法测母血和脐带血抗-HEVIgM/IgG,同时查新生儿肝组织病理改变,免疫组化法查肝内戊型肝炎病毒抗原(HEVAg),用地戈辛(DIG)探针标记HEVcDNA23qbp片段进行原位杂交查肝内HEVRNA。结果母血抗-HEV、IgM/IgG均阳性,脐带血仅抗-HEVIgG阳性,肝组织以变性、炎变为主,新生儿肝内HEVAg阳性率77%(10/13),HEV-RNA阳性率62%(8/13)。结论HEV存在宫内感染

 
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