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四糖为主
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  tetrasaccharide
     The agar oligosaccharides A1(mainly with disaccharidae and tetrasaccharide) and A2 (mainly with hexose and octoses) were prepared from agar under different acid hydrolysis conditions.
     在不同条件下对琼胶进行酸水解,得琼胶寡糖A1(以二、四糖为主)和A2(以己糖、辛糖为主)采用化学发光法和DPPH体系分别研究A1、A2对O2·-、·OH和DPPH3种自由基的清除作用。
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  “四糖为主”译为未确定词的双语例句
     Xylose was not detected by TLC analysis,it was suggested that the xylanase was suitable for(xylooligosaccharides) production.
     该酶水解玉米芯木聚糖生成以木二糖、木三糖和木四糖为主的低聚木糖,薄层层析分析表明不含木糖,适合生产低聚木糖。
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  相似匹配句对
     proliferatum was the predominant species.
     proliferatum为主
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     The important ones are EST database.
     HTGS数据库为主
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     Synthesis of the repeating unit of E. coli K30 antigen
     E. coli K30抗原四糖重复单元的合成
     Sucrose Chemistry :Studies on the Synthesis of Tri- and Tetra-Saccharides
     蔗糖化学─—三糖和四糖合成的研究
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     The Structure Study of a New Saikosaponin
     一种新的四糖柴胡皂苷结构的NMR研究
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  tetrasaccharide
From the results of Smith degradation, O-deacetylation of the polysaccharide, and NMR spectroscopy the following structure of the tetrasaccharide repeating unit of the O-specific polysaccharide was established: where R: -CO-CH2-CH2-CH2-OH.
      
The pseudo-tetrasaccharide acarbose, a potent α-amylase and α-glucosidase inhibitor, does not inhibit AglB but, on the contrary, acarbose is degraded quantitatively by AglB.
      
The resulting glycosyl donor was used in the synthesis of tetrasaccharide asialo-GM1.
      
The polysaccharide was shown to be composed of branched tetrasaccharide repeating units containing two N-acetyl-D-galactosamine residues, D-galactose, D-glucose, and glycerophosphate residues (one of each).
      
The polysaccharide was shown to consist of repeating tetrasaccharide units containing two mannose residues, one N-acetyl-D-glucosamine residue, and one D-glucuronic acid residue.
      
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Aspergillus ustus showing high xylanase activity was isolated from 1144 strains of fungi.The medium for the optimal xylanase production(4% wheat spelt,0.5% glucose,0.4% NaNO 3 and 0.1% NaCl)yielded 2176 u/mL within 6 days of cultivation at 30℃.The enzyme showed a temperature optimum of 55℃ and retained 35% of activity after incubation at 45℃ for 1 h.The enzyme also showed a pH optimum of 5.5 and was stable in the range of pH 5.0 to 8.0.The K m value and V max value calculated were 4.3×10 ...

Aspergillus ustus showing high xylanase activity was isolated from 1144 strains of fungi.The medium for the optimal xylanase production(4% wheat spelt,0.5% glucose,0.4% NaNO 3 and 0.1% NaCl)yielded 2176 u/mL within 6 days of cultivation at 30℃.The enzyme showed a temperature optimum of 55℃ and retained 35% of activity after incubation at 45℃ for 1 h.The enzyme also showed a pH optimum of 5.5 and was stable in the range of pH 5.0 to 8.0.The K m value and V max value calculated were 4.3×10 -3 g/mL and 4.9 mg/(mL·min) for birchwood xylan,respectively.The hydrolysis products from birchwood xylan were xylotriose and xylotetraose.Thus,the enzyme is considered to be an endoglycosidese.

从1144株真菌中筛选到一株产木聚糖酶活力较高的焦曲霉(Aspergilusustus)。该菌株适宜的产酶条件为在4%麸皮液中添加05%葡萄糖,04%硝酸钠及01%氯化钠,30℃振荡培养6d,木聚糖酶活力可达2176u/mL。酶反应的最适温度为55℃,最适pH为55,在pH5~8酶活力稳定。45℃保温1h,酶活力剩余35%。酶水解桦木木聚糖的Km值为43×10-3g/mL,Vmax值为49mg/(mL·min)。酶解产物以木三糖和木四糖为主,表明该酶是一种典型的内切糖苷酶。

The agar oligosaccharides A1(mainly with disaccharidae and tetrasaccharide) and A2 (mainly with hexose and octoses) were prepared from agar under different acid hydrolysis conditions. Using the methods of Luminesecence and DPPH system, the scavenging effects of A1 and A2 on O2·-,·OH and DPPH are determined that A1 and A2 have strong scavenging effects on O2·- with the IC50 at 0.8 and 0.54 mg/mL. The scavenging activities of A1 and A2 on ·OH are lower than those on O2·- and their IC50...

The agar oligosaccharides A1(mainly with disaccharidae and tetrasaccharide) and A2 (mainly with hexose and octoses) were prepared from agar under different acid hydrolysis conditions. Using the methods of Luminesecence and DPPH system, the scavenging effects of A1 and A2 on O2·-,·OH and DPPH are determined that A1 and A2 have strong scavenging effects on O2·- with the IC50 at 0.8 and 0.54 mg/mL. The scavenging activities of A1 and A2 on ·OH are lower than those on O2·- and their IC50 on ·OH are 2.3 and 1.2 mg/mL. A2 has better scavenging ability on O2·-and ·OH than A1. A1 and A2 have certain scavenging activities on DPPH and A1 shows higher scavenging ability than A2, which is quite different from the results in O2·- and · OH. But their abilities are much lower than probucol and Vc, which have very active hydrogens and good scavenging activity on DPPH. It suggests that agar oligosaccharides A1 and A2 have different scavenging abilities in different free radical assays.

在不同条件下对琼胶进行酸水解,得琼胶寡糖A1(以二、四糖为主)和A2(以己糖、辛糖为主)采用化学发光法和DPPH体系分别研究A1、A2对O2·-、·OH和DPPH3种自由基的清除作用。结果表明,A1、A2对3种自由基都有较好的清除作用,而且清除活性随糖浓度的增加而加强。在O2·-体系中,A2的IC50为0.54mg/mL,效果要好于A1(IC50为0.8mg/mL)。在·OH体系中,A2对·OH的清除作用比A1效果好,IC50分别为1.2和2.3mg/mL。2个体系比较发现A1、A2对O2·-的清除作用高于对·OH的清除作用。在DPPH体系中,A1、A2对DPPH有一定的清除,但A1较A2效果好,IC50分别为4和6 4mg/mL。由此可见,琼胶寡糖在不同体系中清除自由基的活性不同。琼胶寡糖在抗氧化方面具有很好的应用价值。

Purification and properties of a xylanase produced by a new thermophilic Paecilomyces thermophila J18 in solid state fermentation was studied.The xylanase with a molecular mass of 26 kDa was then purified by 5.27 fold to(homogeneity) with a recovery of 33.5% by ammonium sulfate precipitation,gel filtration and ion-exchange(chromatography).It exhibited remarkable stability,and remained more than 80% of its original activity after treated at 60℃ for 6 h within pH 6.0-9.0.The xylanase hydrolyzed corncob xylan to...

Purification and properties of a xylanase produced by a new thermophilic Paecilomyces thermophila J18 in solid state fermentation was studied.The xylanase with a molecular mass of 26 kDa was then purified by 5.27 fold to(homogeneity) with a recovery of 33.5% by ammonium sulfate precipitation,gel filtration and ion-exchange(chromatography).It exhibited remarkable stability,and remained more than 80% of its original activity after treated at 60℃ for 6 h within pH 6.0-9.0.The xylanase hydrolyzed corncob xylan to yield mainly xylobiose,xylotriose and(xylotetarose) as end(products).Xylose was not detected by TLC analysis,it was suggested that the xylanase was suitable for(xylooligosaccharides) production.

研究一株新的嗜热拟青霉J18的固体发酵产木聚糖酶的纯化和性质。固体发酵的粗酶液经硫酸铵沉淀、凝胶过滤层析和离子交换层析得到了一种分子量约为26 kDa的电泳纯木聚糖酶,酶活力回收率为33.5%,纯化了5.27倍。该木聚糖酶具有很好的温度和pH稳定性,在pH7.0~pH 9.0下,60℃处理24 h,酶活力能保存80%以上。该酶水解玉米芯木聚糖生成以木二糖、木三糖和木四糖为主的低聚木糖,薄层层析分析表明不含木糖,适合生产低聚木糖。

 
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