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假杂交
相关语句
  false hybrid
     The Study on Determining True and False Hybrid Rice Ⅱ You 63 Using RAPD Molecular Markers
     真、假杂交水稻Ⅱ优63的RAPD鉴定
短句来源
     Study on Determining True or False Hybrid Rice by Using Biotechnology
     利用分子生物学技术鉴别真、假杂交稻的研究
短句来源
     One hundred RAPD primers were used to amplify true and false hybrid rice ⅡYou 63 genomic DNAs in this experiment. The main DNA polymorphisms from the two materials were not found, indicating that true and fasle ⅡYou 63 genetic background are similar.
     真、假杂交水稻Ⅱ优63的RAPD鉴定钱前,陈洪,孙宗修,朱立煌(中国水稻研究所,杭州310006)(中国科学院遗传研究所,北京100101)关键词;
短句来源
     By matching marker genotypes of corresponding F_ 1 , maternal and paternal lines of hybrid rice, high reliability of the maternal lines was verified, data on paternal lines were modified, and a false hybrid was removed. A database containing genotype data of 103 major rice varieties and parental lines at the 24 marker loci was constructed and analyzed.
     通过杂交稻组合与其亲本的标记匹配性检验,确认了杂交稻母本的高真实性,提高了恢复系数据的可靠性,剔除了1个假杂交稻,建立了103个水稻材料×24个标记的主栽水稻品种微卫星标记数据库,并分析了数据库中各个标记、各组材料的多态性表现。
短句来源
  “假杂交”译为未确定词的双语例句
     S1361 and S1375 can distinguish the pepper hybrid from its maternal lines so that it can be used to distinguish true hybrids from self-contaminating progeny.
     S1361、S1375能将辣椒的杂交种与母本相区分,可区分母本自交型假杂交种。
短句来源
     Spraying of 0.25% Basta to hybrid rice seedling, the fake hybrid seedlings were killed by Basta and excluded from population for hybrid seed production to increase hybrid seed purity.
     使用bar基因的恢复系所配制的杂交稻组合,秧苗期喷施0.25%Basta药剂,可以有效清除混杂在杂交稻中的假杂交种,从而保证大田生产的纯度。
短句来源
  相似匹配句对
     northern hybridization.
     Northern杂交
短句来源
     e-Hybrid Rice
     e杂交
短句来源
     The Study on Determining True and False Hybrid Rice Ⅱ You 63 Using RAPD Molecular Markers
     真、杂交水稻Ⅱ优63的RAPD鉴定
短句来源
     Study on Determining True or False Hybrid Rice by Using Biotechnology
     利用分子生物学技术鉴别真、杂交稻的研究
短句来源
     There was no false positive case.
     无阳性。
短句来源
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  false hybrid
Hybrids of four combinations out of six turned out as true hybrids, one as sesquidiploids and the another one as false hybrid plant.
      


One hundred RAPD primers were used to amplify true and false hybrid rice ⅡYou 63 genomic DNAs in this experiment. The main DNA polymorphisms from the two materials were not found, indicating that true and fasle ⅡYou 63 genetic background are similar. However, we had established a PCR system which can be used to determine the seed purity of Shanyou 63 by using a primer P18 to amplify a specific band of 0. 8 kb from restorer line Minghui 63. This technique can also be used to determine true and false ⅡYou 63 correctly...

One hundred RAPD primers were used to amplify true and false hybrid rice ⅡYou 63 genomic DNAs in this experiment. The main DNA polymorphisms from the two materials were not found, indicating that true and fasle ⅡYou 63 genetic background are similar. However, we had established a PCR system which can be used to determine the seed purity of Shanyou 63 by using a primer P18 to amplify a specific band of 0. 8 kb from restorer line Minghui 63. This technique can also be used to determine true and false ⅡYou 63 correctly and fast, verifying that the Shanyou 63 PCR system is suitable to determine true and false hybrid rices using Minghui 63 as restorer line.

真、假杂交水稻Ⅱ优63的RAPD鉴定钱前,陈洪,孙宗修,朱立煌(中国水稻研究所,杭州310006)(中国科学院遗传研究所,北京100101)关键词;杂交水稻,RAPD标记,真假鉴定TheStudyonDeterminingTrueandFalseHy...

ar gene was introduced to immature embroys of a japonica rice cultivar Jingying119 through biolistic system and a transgenic plant B 119 expressing resistance to Basta was obtained. The resistance to herbicide was stably maintained in five consecutive self crossing generations . It is simple and feasible to introduce the bar gene to restorer lines through intercross and backcross. Spraying of 0.25% Basta to hybrid rice seedling, the fake hybrid seedlings were killed by Basta and excluded from population...

ar gene was introduced to immature embroys of a japonica rice cultivar Jingying119 through biolistic system and a transgenic plant B 119 expressing resistance to Basta was obtained. The resistance to herbicide was stably maintained in five consecutive self crossing generations . It is simple and feasible to introduce the bar gene to restorer lines through intercross and backcross. Spraying of 0.25% Basta to hybrid rice seedling, the fake hybrid seedlings were killed by Basta and excluded from population for hybrid seed production to increase hybrid seed purity. It is helpful to clear up unstable fertility when widen the use of sterile lines.

利用基因枪轰击水稻京引119未成熟胚获得转基因水稻植株B-119,经连续自交至T5代仍可稳定保持对Basta的抗性。采用人工杂交、回交转育等途径也可将转基因植株的bar基因导入杂交水稻恢复系。使用bar基因的恢复系所配制的杂交稻组合,秧苗期喷施0.25%Basta药剂,可以有效清除混杂在杂交稻中的假杂交种,从而保证大田生产的纯度。研究结果对于拓宽杂交稻特别是两系杂交稻不育系的应用范围,展现出广泛的应用前景。

Sixty-three major conventional varieties and parental lines of major F_ 1 hybrids used in the commercial rice production in China were assayed with microsatellite markers screened in a previous study and additional markers on four chromosomes. A set of 24 markers was selected and proposed for its application in the variety identification of rice, which are distributed on all the 12 rice chromosomes with two markers on each chromosome. The 63 major varieties and parental lines, as well as 41 major F_ 1 hybrids,...

Sixty-three major conventional varieties and parental lines of major F_ 1 hybrids used in the commercial rice production in China were assayed with microsatellite markers screened in a previous study and additional markers on four chromosomes. A set of 24 markers was selected and proposed for its application in the variety identification of rice, which are distributed on all the 12 rice chromosomes with two markers on each chromosome. The 63 major varieties and parental lines, as well as 41 major F_ 1 hybrids, were genotyped with the markers, and alleles detected at each marker locus were verified. By matching marker genotypes of corresponding F_ 1 , maternal and paternal lines of hybrid rice, high reliability of the maternal lines was verified, data on paternal lines were modified, and a false hybrid was removed. A database containing genotype data of 103 major rice varieties and parental lines at the 24 marker loci was constructed and analyzed. The influence of the authenticity of rice materials on the database quality was discussed, and a threshold for microsatellite marker-based variety identification in rice and an approach for authentification of rice materials and genotype data were proposed.

以前期筛选的微卫星标记为基础,并在4个染色体上增加新标记,进一步检测了63个主栽常规稻品种和杂交稻亲本,推荐了24个微卫星标记(每条染色体2个)作为水稻品种鉴别的标记;除了63个常规稻品种和杂交稻亲本外,这24个标记还应用于检测41个主栽杂交稻组合,并确定了各个标记在试验材料中检测到的等位基因类型。通过杂交稻组合与其亲本的标记匹配性检验,确认了杂交稻母本的高真实性,提高了恢复系数据的可靠性,剔除了1个假杂交稻,建立了103个水稻材料×24个标记的主栽水稻品种微卫星标记数据库,并分析了数据库中各个标记、各组材料的多态性表现。此外,还讨论了材料真实性和典型性对数据库构建的影响,提出了水稻品种间差异判别标准的建议及其应用风险和解决途径。

 
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