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葡萄糖激酶基因启动子
相关语句
  glucokinase gene promoter
     Glucokinase gene promoter G/A variant is associated with higher fasting glucose levels
     葡萄糖激酶基因启动子G/A变异与空腹高血糖相关
短句来源
     The Study of Test Condition with PCR-RFLP Method to Detect the Variant-30 Position of the Glucokinase Gene Promoter
     PCR-RFLP法检测葡萄糖激酶基因启动子-30位点变异的实验条件研究
短句来源
     The Study of Test Condition in the Variation at 30-Position of the Glucokinase Gene Promoter by PCR-RFLP
     PCR-RFLP法检测葡萄糖激酶基因启动子-30位点变异的实验条件研究
短句来源
     Objective To confirm the optimal test condition of Alw21 I digestion of the -30position of the glucokinase gene promoter.
     目的确定用Alw21I限制性内切酶切割PCR(聚合酶链反应)扩增葡萄糖激酶基因启动子区-30位点的最适实验条件。
短句来源
  “葡萄糖激酶基因启动子”译为未确定词的双语例句
     Glucokinase Gene Promoter-30 G/A Variant Associated with Type II Diabetic Mellitus
     葡萄糖激酶基因启动子区-30位G/A变异与2型糖尿病的相关性研究
短句来源
     Objective:To confirm the optimal test condition of the promoter region with PCR method and Alw21I digestion of the-30 position of the glucokinase gene promter.
     目的 :确定PCR扩增葡萄糖激酶基因启动子区以及用Alw2 1I内切酶切割 -3 0位点的最佳实验条件 .
短句来源
  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Glucokinase gene promoter G/A variant is associated with higher fasting glucose levels
     葡萄糖激酶基因启动子G/A变异与空腹高血糖相关
短句来源
     SEPARATION OF O.violacecus GENE PROMOTERS
     诸葛菜基因启动子的分离
短句来源
     The Eenvironmental Response Promotor of Plant Genes
     植物基因环境效应启动子
短句来源
     The Study of Test Condition with PCR-RFLP Method to Detect the Variant-30 Position of the Glucokinase Gene Promoter
     PCR-RFLP法检测葡萄糖激酶基因启动子-30位点变异的实验条件研究
短句来源
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Objective:To confirm the optimal test condition of the promoter region with PCR method and Alw21I digestion of the-30 position of the glucokinase gene promter.Method:The systemic study was processed for the factor affecting PCR and restricted digestion.Result:The optimal primer concentration is 0.3 μmol/L;the satisfactory Taq enzyme amount is 1U;the optimal dNTP concentration is 167 μmol/L;the optimal Mg 2+ concentration is 1.5 mmol/L.In 20 μL digestion solution,the optimal amplified production is 5 μL;the...

Objective:To confirm the optimal test condition of the promoter region with PCR method and Alw21I digestion of the-30 position of the glucokinase gene promter.Method:The systemic study was processed for the factor affecting PCR and restricted digestion.Result:The optimal primer concentration is 0.3 μmol/L;the satisfactory Taq enzyme amount is 1U;the optimal dNTP concentration is 167 μmol/L;the optimal Mg 2+ concentration is 1.5 mmol/L.In 20 μL digestion solution,the optimal amplified production is 5 μL;the optimal enzyme amount is 5 U;the digestion time should exceed 9 hours.The examination will fail if the test condition deflect the above optimal situation.

目的 :确定PCR扩增葡萄糖激酶基因启动子区以及用Alw2 1I内切酶切割 -3 0位点的最佳实验条件 .方法 :对影响PCR反应以及酶切的实验因素进行系统研究 .结果 :最佳引物浓度为 0 3 μmol/L ;以 1U酶量效果最满意 ;最适dNTP浓度为 1 67μmol/L ;最适镁离子浓度为 1 5mmol/L . 2 0 μL的酶切体系中 ,最佳酶切产物为 5 μL,最佳内切酶量为 5U ,酶切时间应超过 9h .以上参数偏离最适条件将会导致实验失败 .

Objective To confirm the optimal test condition of Alw21 I digestion of the -30position of the glucokinase gene promoter.Methods Setting a test system in different amount of amplified production,concentration of restriction endonuclease and the endonuclease digestion time,analyse the consequence.Results In 20 μl digestion solution,the optimal amplified production was 5 μl;the optimal enzyme amount was 5 U;the digestion time should exceed 9 hours.Conclusion The examination will fail if the test condition is deflect...

Objective To confirm the optimal test condition of Alw21 I digestion of the -30position of the glucokinase gene promoter.Methods Setting a test system in different amount of amplified production,concentration of restriction endonuclease and the endonuclease digestion time,analyse the consequence.Results In 20 μl digestion solution,the optimal amplified production was 5 μl;the optimal enzyme amount was 5 U;the digestion time should exceed 9 hours.Conclusion The examination will fail if the test condition is deflect the above optimal situation.

目的确定用Alw21I限制性内切酶切割PCR(聚合酶链反应)扩增葡萄糖激酶基因启动子区-30位点的最适实验条件。方法按一定梯度设定酶切所用扩增产物量、内切酶的浓度、酶切时间,用2g/dl低熔点琼脂糖凝胶(含0.5μg/ml的溴乙锭)电泳,透射式紫外灯下观察酶切结果。结果20μl的酶切体系中,最适酶切产物为5μl,最适内切酶量为5U,酶切时间应超过9h。结论参数偏离最适条件将会导致实验失败。

 
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