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dna复合
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  dna complex
     Methods:The lipid DNA complex was added to B16F1 cells of 106. The suspernatant of cell culture was collected and replaced each day for 5 days and SEAP levels were assayed with SEAP kit.
     方法 :脂质体和质粒DNA复合后 ,转染 10 6 B16F1细胞 ,连续 5d收集并换细胞培养液 ,并检测其中的SEAP酶活性。
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  “dna复合”译为未确定词的双语例句
     Alginate/PEI/DNA polyplexes: a new gene delivery system
     藻酸盐/PEI/DNA复合载体作为一种新型基因递送系统(英文)
短句来源
     Further, the expressions of P815A and GM-CSF mediated by peptide-DNA vaccine particles were accessed by cell transfection and Western blotting.
     细胞转染、Western Blotting鉴定肽-DNA复合疫苗介导P815A、GM-CSF基因表达的有第二军医人学硕士研究生论文效性; ;
短句来源
     As a test system, we chose a CTL epitope from the well-defined tumor Ag, P815A, as atarget and synthesized a bifunctional linear cationic peptide, which comprises a P815A CTL epitope and a DNA-binding moiety consisting of polylysine residues.
     本研究以肿瘤相关抗原P815A为模式抗原,合成含有P815A CTL表位和多聚赖氨酸DNA结合区的双功能线性肽,并以该多肽对编码P815A和GM-CSF细胞因子的真核双表达质粒进行包装,进而形成肽-DNA复合疫苗颗粒。
短句来源
     Methods:The lipid-DNA complex was added to B16F1 cells of 10 6.The culture medium of cells was replaced each day for 5 days and mGMCSF expression level was assayed.
     方法 :脂质体和质粒DNA复合后 ,转染 10 6B16F1细胞 ,连续 5d换细胞培养液 ,并检测其中的mGM CSF含量。
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     Conditions exploration about microsatellite DNA composite amplication of Tibetan goats
     藏山羊微卫星DNA复合扩增的条件探索
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     映射的微分
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     DNA,respectively.
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     Preliminary study on the immunization effect of the combined HCV/HBV DNA immunization
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     HIV多表位DNA疫苗的设计及免疫研究
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  dna complex
Binding of Mg2+ to the C7H5O2Hg-DNA complex caused appropriate changes in secondary structure of the substrate.
      
Conservative contacts in the NF-κB dimer-DNA complex composed of p50 and/or p65 NF-κB subunit and DNA sequences like 5'-GGGAMWTTCC-3' were revealed.
      
The influence of Zn(II) on the combination of CS and calf thymus DNA (CT DNA) was studied using fluorescence spectrophotometry, and the formation of binary CS-Zn(II) and CS-CT DNA complexes and ternary CS-Zn(II)-CT DNA complex was studied.
      
Synthesis of a Conducting Interpolymer Polyaniline-DNA Complex
      
The stoichiometry of the strongly fluorescent dye-DNA complex was determined.
      
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The effect of heparin on sperm normal shape, total count, motility and concentration of protamine was studied. The results showed that the concentration was decreased after injecting heparin to mouse testes. A good relation ship was observed between the dose of heparin and the concentration of prota mine(r =-0.89) and the abnormal sperm shape (r = 0.74). There is no such relationship between the dose of heparin and total sperm count and motility. It shows that heparin has an effect on protamine concentration...

The effect of heparin on sperm normal shape, total count, motility and concentration of protamine was studied. The results showed that the concentration was decreased after injecting heparin to mouse testes. A good relation ship was observed between the dose of heparin and the concentration of prota mine(r =-0.89) and the abnormal sperm shape (r = 0.74). There is no such relationship between the dose of heparin and total sperm count and motility. It shows that heparin has an effect on protamine concentration and then effect the normal sperm shape. These may be the reason that causes infertility.

肝素类(?)阴离子化合物能与鱼精蛋白特异结合,破坏精子核中鱼精蛋白-DNA复合结构,使精子核膨胀.本研究利用这一特性对小鼠睾丸注射大刘量肝素后,观察精子形态,活动度及总计数等常规指标的变化,以确定肝素对鱼精蛋白,对精子生成及成熟后的含量,研究结果证明,小鼠睾丸注射大剂量肝素后,可以明显减少核内鱼精蛋白的影响,畸形率增加,活动度在高剂量组虽有降低.但不明显,也无剂量-反应关系,总计数无明显变化,结果认为,肝素影响鱼精蛋白的核内含量变化,进而影响其精子形态的变化,而导致生育力的下降.

To study the relationship on infection of Ure-aplasm Urealyticum (UU), Chlamydia Trachomatis (CT) and threatened abortion. UU and CT in cervical discharge were detected by PCR technique in 63 threatened abortion patients, while culture was performed and 40 normal pregnancy cases were used as controls. The detective rates of UU-DNA and CT-DNA were 50. 8% and 37. 7% respectively in threatened abortion group. Compared with control group, there were significant differences. (P< 0. 05, P<0. 05). The detective rate...

To study the relationship on infection of Ure-aplasm Urealyticum (UU), Chlamydia Trachomatis (CT) and threatened abortion. UU and CT in cervical discharge were detected by PCR technique in 63 threatened abortion patients, while culture was performed and 40 normal pregnancy cases were used as controls. The detective rates of UU-DNA and CT-DNA were 50. 8% and 37. 7% respectively in threatened abortion group. Compared with control group, there were significant differences. (P< 0. 05, P<0. 05). The detective rate of mixed UU and CT is 15. 8%, the cultural positive rate of UUis 46. 0%. UU and/or CT infection in cervix is closely related with threatened abortion, it is one of the factors which may cause threatened abortion.

为研究宫颈解脲支原体(UU)与沙眼衣原体(CT)感染与先兆流产的关系,对63例先兆流产要求保胎者和40例正常妊娠者取宫颈分泌物,用PCR技术对UU、CT感染进行研究,同时取先兆流产患者宫颈分泌物进行UU培养和药敏试验。结果:先兆流产组UU—DNA、CT—DNA阳性率分别为50.8%,31.7%,与对照组相比有显著性差异(P<0.05)。先兆流产组UU—DNA与CT—DNA复合阳性率15.8%。UU培养阳性率46.0%,与PCR法UU—DNA检出率相比差异无显著性(P>0.05)结果显示,宫颈UU和(或)CT感染与先兆流产密切相关,是导致先兆流产的病因之一。

Objective: To compare the expression kinetics of SEAP gene from B16F1 cells transfected by cationic lipid and transducted by recombinant adenovirus in order to explore the potential application of two gene transfer vector: cationic lipid and recombinant adenovirus.Methods:The lipid DNA complex was added to B16F1 cells of 106. The suspernatant of cell culture was collected and replaced each day for 5 days and SEAP levels were assayed with SEAP kit.The recombinant adenovirus at m.o.i 1000?m.o.i2000?m.o.i3000?m.o.i4000...

Objective: To compare the expression kinetics of SEAP gene from B16F1 cells transfected by cationic lipid and transducted by recombinant adenovirus in order to explore the potential application of two gene transfer vector: cationic lipid and recombinant adenovirus.Methods:The lipid DNA complex was added to B16F1 cells of 106. The suspernatant of cell culture was collected and replaced each day for 5 days and SEAP levels were assayed with SEAP kit.The recombinant adenovirus at m.o.i 1000?m.o.i2000?m.o.i3000?m.o.i4000 was inoculated respectively to B16F1 cells of 106. Results: The expression level of SEAP decreased from 821.6 μUnit/ml on day 1 to 275.2 μUnit/ml on day 5 gradually.The expression level of SEAP elevated gradually with increasing m.o.i of inoculated adenovirus and time going. In the group inoculated with adenovirus at m.o.i 4000,SEAP level increased from 93.9 μUnit/ml on day 1 to 1281.1 μUnit/ml on day 3 and 1240.4 μUnit/ml on day 5.Conclusion:Cationic lipid and recombinant adenovirus,as two different gene transfer vectors,could deliver foreign SEAP genes into B16F1 cells in which SEAP expressed efficiently.but both expression kinetics are completely different.

目的 :探讨两种基因转移载体脂质体转染的和重组腺病毒收集并转导的SEAP基因在B16F1细胞中的表达动态。方法 :脂质体和质粒DNA复合后 ,转染 10 6 B16F1细胞 ,连续 5d收集并换细胞培养液 ,并检测其中的SEAP酶活性。将重组腺病毒以 10 0 0m .o .i、2 0 0 0m .o .i、3 0 0 0m .o .i、4 0 0 0m .o .i接种B16F1细胞 ,连续 5d收集并换细胞培养液 ,并检测其中SEAP的含量。结果 :随着时间的推移 ,脂质体转染的SEAP的表达逐渐下降 ,由第 1天的 82 1.6μUnit/ml下降到第 5天的 2 75 .2 μUnit/ml。腺病毒转导的SEAP的表达却是逐渐上升。并随接种毒量的增加而上升。且第三、四天表达达最高后 ,能保持较稳定的水平。接种 4 0 0 0m .o .i组 ,SEAP的表达由第 1天的93 .9μUnit/ml上升到第 3天 12 81.1μUnit/ml。到第 5天仍为 12 4 0 .4 μUnit/ml。结论 :脂质体和重组腺病毒作为不同的基因转移载体都能有效地介导外源基因的转移入靶细胞 ,并使外源基...

目的 :探讨两种基因转移载体脂质体转染的和重组腺病毒收集并转导的SEAP基因在B16F1细胞中的表达动态。方法 :脂质体和质粒DNA复合后 ,转染 10 6 B16F1细胞 ,连续 5d收集并换细胞培养液 ,并检测其中的SEAP酶活性。将重组腺病毒以 10 0 0m .o .i、2 0 0 0m .o .i、3 0 0 0m .o .i、4 0 0 0m .o .i接种B16F1细胞 ,连续 5d收集并换细胞培养液 ,并检测其中SEAP的含量。结果 :随着时间的推移 ,脂质体转染的SEAP的表达逐渐下降 ,由第 1天的 82 1.6μUnit/ml下降到第 5天的 2 75 .2 μUnit/ml。腺病毒转导的SEAP的表达却是逐渐上升。并随接种毒量的增加而上升。且第三、四天表达达最高后 ,能保持较稳定的水平。接种 4 0 0 0m .o .i组 ,SEAP的表达由第 1天的93 .9μUnit/ml上升到第 3天 12 81.1μUnit/ml。到第 5天仍为 12 4 0 .4 μUnit/ml。结论 :脂质体和重组腺病毒作为不同的基因转移载体都能有效地介导外源基因的转移入靶细胞 ,并使外源基因在靶细胞内获得有效地表达。但表达的动态过程完全不同

 
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