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牛促卵泡激素
相关语句
  bovine follicle stimulating hormone
     Introduction and Expression of cDNA for β Subunit of Bovine Follicle Stimulating Hormone in Mammalian Cells
     牛促卵泡激素β亚基基因在哺乳动物细胞中的导入与表达
短句来源
     Nucleotide Sequence of Cloned cDNA for α Subunit of Bovine Follicle Stimulating Hormone
     牛促卵泡激素α亚基cDNA的克隆与序列分析
短句来源
     Bovine follicle stimulating hormone (FSH) gene was screened using synthetic oligonucleotide probe and Polymerase Chain Reaction (PCR).
     用标记的牛促卵泡激素基因的寡核苷酸片段进行杂交,从文库中筛选到几个阳性克隆,其中一个含有全长的牛促卵泡激素基因编码序列。
短句来源
     Based on hybridomas highly specific to bovine follicle stimulating hormone(bFSH) and bovine luteinizing hormone(bLH) obtained before, two monoclonal antibodies(McAbs)against bFSH and bLH respectively were purified, and a new technique of immuno affinity filtering was established.
     在本室已获得高特异抗牛促卵泡激素 (bFSH)和抗牛促黄体激素 (bLH)杂交瘤细胞的基础上 ,制备纯化抗bFSH和抗bLH两种单克隆抗体 (McAbs) ,并进而建立了一种单抗免疫亲和渗滤新技术。
短句来源
  “牛促卵泡激素”译为未确定词的双语例句
     CLONING, SCREENING AND SEQUENCE ANALYSIS OF BOVINE FOLLICLE-STIMULATING HORMONE cDNA
     牛促卵泡激素基因克隆筛选和序列分析
短句来源
     By using PCR technique, one 387bp DNA fragment was amplified from eDNA, which was then cloned into pUC19, M13mp18 and M13mp19 for DNA sequence analysis, results show that the DNA fragment is coding for a FSH of 109 amino acids and a signal peptide of 20 amino acids which sequence is the same as the published one.
     利用PCR扩增技术从cDNA中扩增出了387bp的牛促卵泡激素基因,序列分析表明,10号克隆中的牛促卵泡激素基因含有起始密码ATG及终止密码TAA,所编码的氨基酸序列与天然牛促卵泡激寡素的氨基酸序列相同。
短句来源
  相似匹配句对
     CLONING, SCREENING AND SEQUENCE ANALYSIS OF BOVINE FOLLICLE-STIMULATING HORMONE cDNA
     促卵泡激素基因克隆筛选和序列分析
短句来源
     John Bull
     约翰
短句来源
     Effect of FSH and Insulin on Bovine Granulosa Cells in Long Term Serum-Cultrue
     促卵泡激素和胰岛素对卵泡颗粒细胞长期培养的影响
短句来源
     Studies on in Vitro Fertilization in Bovine
     体外受精的研究
短句来源
     Purification of Follicle-Stimulating Hormone by Radial-Flow Chromatography
     促卵泡激素的径向色谱纯化工艺
短句来源
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Total mRNA of Bovine Pituitary was isolated and purified. The eDNA library was then constructed. Bovine follicle stimulating hormone (FSH) gene was screened using synthetic oligonucleotide probe and Polymerase Chain Reaction (PCR). By using PCR technique, one 387bp DNA fragment was amplified from eDNA, which was then cloned into pUC19, M13mp18 and M13mp19 for DNA sequence analysis, results show that the DNA fragment is coding for a FSH of 109 amino acids and a signal peptide of 20 amino acids which sequence...

Total mRNA of Bovine Pituitary was isolated and purified. The eDNA library was then constructed. Bovine follicle stimulating hormone (FSH) gene was screened using synthetic oligonucleotide probe and Polymerase Chain Reaction (PCR). By using PCR technique, one 387bp DNA fragment was amplified from eDNA, which was then cloned into pUC19, M13mp18 and M13mp19 for DNA sequence analysis, results show that the DNA fragment is coding for a FSH of 109 amino acids and a signal peptide of 20 amino acids which sequence is the same as the published one.

从牛垂体中分离出总mRNA,经逆转录酶及大肠杆菌DNA聚合酶合成双链cDNA,经T4DNA聚合酶切成平末端后与Sma I酶切的pUC19连接,转化JM83,构建了脑垂体mRNA的cDNA文库。用标记的牛促卵泡激素基因的寡核苷酸片段进行杂交,从文库中筛选到几个阳性克隆,其中一个含有全长的牛促卵泡激素基因编码序列。利用PCR扩增技术从cDNA中扩增出了387bp的牛促卵泡激素基因,序列分析表明,10号克隆中的牛促卵泡激素基因含有起始密码ATG及终止密码TAA,所编码的氨基酸序列与天然牛促卵泡激寡素的氨基酸序列相同。

Total RNA was prepared from pituitary of bovine.mRNA was isolated from the total RNA. cDNA was synthesized as template used in PCR for amplification of cDNA for α subunit of bovine follicle stimulating hormone. Sequence analysis showed that the obtained cDNA fragment is 380 bp and all these nucleotides except one coding for Lys of No24 are as same as that Erwin reported. Comparison of bovine, human and rodent indicated that the amino acid and nucleotide sequences of the α subunit in these mammalian...

Total RNA was prepared from pituitary of bovine.mRNA was isolated from the total RNA. cDNA was synthesized as template used in PCR for amplification of cDNA for α subunit of bovine follicle stimulating hormone. Sequence analysis showed that the obtained cDNA fragment is 380 bp and all these nucleotides except one coding for Lys of No24 are as same as that Erwin reported. Comparison of bovine, human and rodent indicated that the amino acid and nucleotide sequences of the α subunit in these mammalian species are highly conservative.

从牛脑垂体中提取总RNA,分离mRNA,反转录获得cDNA,PCR扩增获得长为380bp的牛促卵泡激素α亚基cDNA片段,将它克隆于pUC19中,进行序列分析,结果表明:所克隆的α亚基基因编码区序列与Erwin所发表的序列基本相同,仅编码第24位赖氨酸的密码有差异,同时将所获基因的核苷酸序列及相应氨基酸序列与人、啮齿类的同类基因相比较,具有很高的同源性.

Based on hybridomas highly specific to bovine follicle stimulating hormone(bFSH) and bovine luteinizing hormone(bLH) obtained before, two monoclonal antibodies(McAbs)against bFSH and bLH respectively were purified, and a new technique of immuno affinity filtering was established. The above two bovine reproductive hormones were successfully prepared with the procedure. 3.3mg affinity purified bFSH and 11.1mg affinity purified bLH were produced from 7g powder of bovine pituitary glands, and the recovery rates...

Based on hybridomas highly specific to bovine follicle stimulating hormone(bFSH) and bovine luteinizing hormone(bLH) obtained before, two monoclonal antibodies(McAbs)against bFSH and bLH respectively were purified, and a new technique of immuno affinity filtering was established. The above two bovine reproductive hormones were successfully prepared with the procedure. 3.3mg affinity purified bFSH and 11.1mg affinity purified bLH were produced from 7g powder of bovine pituitary glands, and the recovery rates were 85 % and 82 % separately.

在本室已获得高特异抗牛促卵泡激素 (bFSH)和抗牛促黄体激素 (bLH)杂交瘤细胞的基础上 ,制备纯化抗bFSH和抗bLH两种单克隆抗体 (McAbs) ,并进而建立了一种单抗免疫亲和渗滤新技术。使用该技术成功地制备了上述两种牛生殖激素 ,从 7g牛垂体干粉可提取 3.3mg亲和纯bFSH和 1 1 .1mg亲和纯bLH。回收率分别为 85 %和 82 %。

 
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