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猪戊型肝炎
相关语句
  swine hepatitis e virus infection
     Serology Investigation and Genotype Analysis of Swine Hepatitis E Virus Infection in Xinjiang Region
     新疆猪戊型肝炎的血清流行病学调查及病毒的基因型分析
短句来源
     Epidemic Investigation of Swine Hepatitis E Virus Infection in Xinjiang Region
     新疆地区猪戊型肝炎血清流行病学调查
短句来源
  “猪戊型肝炎”译为未确定词的双语例句
     Investigation on HEV infection in swine and analysis with partial sequences of swine HEV virus in Shanghai
     上海地区猪戊型肝炎感染状况及病毒序列分析
短句来源
     Progress on Swine Hepatitis E
     猪戊型肝炎研究进展
短句来源
     The Distribution of Hepatitis E and Clone of HEV ORF2 and the Constriction of Prokaryotic Vector PET-30 a(+) of Swine in China
     中国猪戊型肝炎(HE)的分布及HEV ORF2片段的基因克隆与原核表达载体的构建
短句来源
     [Objective] To investigate the HEV infection and its genotype of swine in Shanghai region and to explore the possible relationship between swine and human HEV infections.
     [目的] 调查上海地区猪戊型肝炎感染现况,掌握上海市猪感染戊型肝炎病毒的型别,以进一步探讨猪戊型肝炎感染与病人感染的可能关系。
短句来源
     Swine hepatitis E is a newly identified disease that is caused by hepatitis E virus(HEV). HEV is a single-strand RNA virus without an envelope. The genome of HEV is approximate 7.2 kb to 7.4 kb.
     猪戊型肝炎是一种新发现的传染病,其病原戊型肝炎病毒(HEV)是一种无囊膜的RNA病毒,基因组全长为7.2 kb~7.4 kb。
短句来源
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  相似匹配句对
     Progress on Swine Hepatitis E
     肝炎研究进展
短句来源
     Epidemic Investigation of Swine Hepatitis E Virus Infection in Xinjiang Region
     新疆地区肝炎血清流行病学调查
短句来源
     Studies on hepatitis E in China
     我国肝炎研究
短句来源
     The characteristics in elderly hepatitis E patienrs
     老年肝炎的特点
短句来源
     Studies on etiology and vaccines of hepatitis E.
     重视肝炎研究
短句来源
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Objectives To investigate HEV infection in swine and the genotype relationship between swine and human HEV. Methods Anti-HEV IgG antibody was detected in the sera of swine using enzyme linked-immunoassay (EIA), and HEV RNA was amplified by reverse transcription nested polymerase chain reaction (RT-nPCR). The Vector NTI Suite 7 and TreeView softwares were used for nucleotide sequences phylogenetic analysis of HEV isolated from human and swine. Results The anti-HEV IgG positive rate was 16.67% (18/108). Among...

Objectives To investigate HEV infection in swine and the genotype relationship between swine and human HEV. Methods Anti-HEV IgG antibody was detected in the sera of swine using enzyme linked-immunoassay (EIA), and HEV RNA was amplified by reverse transcription nested polymerase chain reaction (RT-nPCR). The Vector NTI Suite 7 and TreeView softwares were used for nucleotide sequences phylogenetic analysis of HEV isolated from human and swine. Results The anti-HEV IgG positive rate was 16.67% (18/108). Among the 18 anti-HEV IgG positive sera, 2 sequences (11.11%, called S18 and S43, respectively) of HEV ORF1 (102-387 bp) were amplified, with the identity of 99% between them. They had 76%-77%, 78%, 76%-79%, 85%-86%, 77%, 80%, 79% and 75%-79% homology at the nucleotide level with human HEV genotypes 1-8, respectively. One (S18) of them was also amplified out in ORF2 region (5 994-6 297 bp) and showed 76%-78%, 74%, 74%-77%, and 85%-94% identity with human HEV genotypes 1-4 at the nucleotide level, respectively. Conclusion HEV sequences isolated from swine belong to human HEV genotype 4.

目的 了解猪戊型肝炎病毒(HEV)感染情况,探讨猪HEV基因型与人HEV的关系。方法用酶联免疫吸附法(ELISA)和逆转录巢式聚合酶链反应法(RT-nPCR)分别检测猪血清抗-HEV IgG抗体和猪HEV RNA,并用Vector NTI Suite 7和TreeView软件进行人与猪HEV核酸序列和基因进化树分析。结果 猪抗-HEV抗体阳性率为16.67%(18/108),并从18份抗-HEV IgG阳性的猪血清中扩增到2株(11.11%,分别命名为S18和S43)HEV 开放读码框(ORF)1片段(102~387bp),两者核苷酸序列同源性为99%,与HEV基因型1~8的核苷酸序列同源性分别为76%~77%、78%、76%~79%、85%~86%、77%、80%、79%和75%~79%。其中1株(S18)还扩增出HEV ORF2片段(5994~6297bp),与HEV基因型1~4的核苷酸序列同源性分别为76%~78%、74%、74%~77%、85%~94%。结论 分离的2株猪HEV属于HEV基因型4。

To analyze the full length sequence of swCH25, isolated from a pig fecal sample in Xinjiang in 2001, eight overlapping fragments of swCH25 genomes were amplified with reverse transcription nested polymerase chain reaction (RT-nPCR) and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD18-T Vector and sequenced. The result showed that its genome consisted of 7 243 nt excluding the poly(A)tail, and contained three ORFs(ORFs1-3) that encoded proteins of 1 707, 674 and 114aa. Sequence...

To analyze the full length sequence of swCH25, isolated from a pig fecal sample in Xinjiang in 2001, eight overlapping fragments of swCH25 genomes were amplified with reverse transcription nested polymerase chain reaction (RT-nPCR) and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD18-T Vector and sequenced. The result showed that its genome consisted of 7 243 nt excluding the poly(A)tail, and contained three ORFs(ORFs1-3) that encoded proteins of 1 707, 674 and 114aa. Sequence analysis revealed that the overall nucleotide sequence identity of swCH25 was 73.5%-75.7% and 83.5%-89.8% with HEV genotypeⅠ-Ⅲ and Ⅳ, respectively. Compared with HEV genotypesⅠ-Ⅲ and Ⅳ,the ORF1 of swCH25 shared 71.6%-74% and 82.3%-89.4% sequence identity at nucleotide level and 80.7%-86.1% and 94.3%-96% identity at amino acid level, the ORF2 shared 78.4%-81.3% and 87.1%-90.9% sequence identity at nucleotide level and 89.7%-93.8% and 97.2%-98.2% identity at amino acid level and the ORF3 shared 84.4%-87.3% and 95.4-96.8% sequence identity at nucleotide level and 74.8%-83.2% and 93.8%-97.4% identity at amino acid level. The phylogenetic tree constructed based on the full genomic sequence confirmed that swCH25 belong to genotype Ⅳ and were most closely related to T1, a prototype human HEV strain of genotype Ⅳ isolated from patients with acute hepatitis in China. This finding further supports the notion that the hepatitis E is a zoonosis.

设计18对PCR引物分片段扩增猪戊型肝炎病毒新疆分离株swCH25全基因,对两末端采用末端快速扩增方法(RACE)进行扩增,扩增产物克隆到pMD18-T载体并测序。用DNAstar软件进行序列同源性分析,PHYLIP软件绘制基因进化树,在全基因序列的基础上比较猪HEV与其它HEV基因型的差异和人源HEV的关系。结果显示swCH25全序列除去3′poly(A)尾,由7243个核苷酸组成,ORF1~3分别编码含1070,674和114个氨基酸的蛋白质。全基因序列与HEVⅠ、Ⅱ和Ⅲ型同源性73.5%~75.7%,与Ⅳ型的同源性为83.5%~89.8%,其中与Ⅳ型中国人源T1株最高,达89.8%。ORF1与Ⅰ~Ⅲ和Ⅳ型的核苷酸同源性分别为71.6%~74%和82.3%~89.4%,氨基酸同源性为80.7%~86.1%和94.3%~96.0%;ORF2与Ⅰ~Ⅲ和Ⅳ型的核苷酸同源性分别为78.4%~81.3%和87.1%~90.9%,氨基酸同源性为89.7%~93.8%和97.2%~98.2%;ORF3与Ⅰ~Ⅲ和Ⅳ型的核苷酸同源性分别为84.4%~87.3%和95.4%~96.8%,氨基酸同源性为74.8%~...

设计18对PCR引物分片段扩增猪戊型肝炎病毒新疆分离株swCH25全基因,对两末端采用末端快速扩增方法(RACE)进行扩增,扩增产物克隆到pMD18-T载体并测序。用DNAstar软件进行序列同源性分析,PHYLIP软件绘制基因进化树,在全基因序列的基础上比较猪HEV与其它HEV基因型的差异和人源HEV的关系。结果显示swCH25全序列除去3′poly(A)尾,由7243个核苷酸组成,ORF1~3分别编码含1070,674和114个氨基酸的蛋白质。全基因序列与HEVⅠ、Ⅱ和Ⅲ型同源性73.5%~75.7%,与Ⅳ型的同源性为83.5%~89.8%,其中与Ⅳ型中国人源T1株最高,达89.8%。ORF1与Ⅰ~Ⅲ和Ⅳ型的核苷酸同源性分别为71.6%~74%和82.3%~89.4%,氨基酸同源性为80.7%~86.1%和94.3%~96.0%;ORF2与Ⅰ~Ⅲ和Ⅳ型的核苷酸同源性分别为78.4%~81.3%和87.1%~90.9%,氨基酸同源性为89.7%~93.8%和97.2%~98.2%;ORF3与Ⅰ~Ⅲ和Ⅳ型的核苷酸同源性分别为84.4%~87.3%和95.4%~96.8%,氨基酸同源性为74.8%~83.2%和93.8%~97.4%,基因进化树显示swCH25与基因Ⅳ型人源T1株最接近,在同一分支上。swCH25株是国内第一个测出全基因序列的猪戊型肝炎病毒。

Purpose To investigate the HEV infection in swine and the genotype relationship between swine HEV and human HEV. Methods HEV RNA was detected using RT-nPCR with ORF2 primers.The positive PCR products were cloned and sequenced. Results Totally 13 of 132(9.85%) pigs' stool samples,11 of 160 pigs' bile samples were positive for HEV RNA.The sequence analysis showed that the identity at nucleotide level was 90.0%—100.0% among them.They had 88.7%—100.0% homology at the nucleotide level with the HEV isolated from local...

Purpose To investigate the HEV infection in swine and the genotype relationship between swine HEV and human HEV. Methods HEV RNA was detected using RT-nPCR with ORF2 primers.The positive PCR products were cloned and sequenced. Results Totally 13 of 132(9.85%) pigs' stool samples,11 of 160 pigs' bile samples were positive for HEV RNA.The sequence analysis showed that the identity at nucleotide level was 90.0%—100.0% among them.They had 88.7%—100.0% homology at the nucleotide level with the HEV isolated from local people.They shared 78.7%—84.0%,80.0%—85.3%, 76.0%—83.3% and 84.7%—95.3% nucleotide sequence identity with HEV genotype I,Ⅱ,Ⅲ and Ⅳ,respectively,in the region(nt6317—6466). Conclusions The hepatitis E virus circulated in human and swine in the area belonged to genotype Ⅳ.

目的调查浙江省某农村地区猪群中戊型肝炎病毒的感染状况,探讨猪戊型肝炎病毒与人戊型肝炎病毒的关系。方法应用逆转录-巢式聚合酶链法(RTnPCR)对当地某养猪场的猪粪便标本和生猪屠宰中心的猪胆汁标本进行HEVRNA的检测,并对部分阳性标本进行克隆测序和序列分析。结果132份猪粪便标本中13份为HEVRNA阳性,阳性率为9.85%;160份猪胆汁标本中有11份为阳性,阳性率为6.88%。序列分析发现猪HEV相互之间在ORF2部分区域(nt6317~6466)的核苷酸序列的同源性为90.0%~100.0%,与当地人株核苷酸序列的同源性为88.7%~100.0%,与Ⅰ、Ⅱ、Ⅲ和Ⅳ型的同源性分别为78.7%~84.0%,80.0%~85.3%,76.0%~83.3%和84.7%~95.3%。结论该地区猪HEV与当地人HEV同属HEVⅣ型。

 
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