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微移植
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  micro-transplantation
     The purpose of the present study was to investigate the survival, migration and differentiation of neural stem cells which were isolated and expanded in vitro from mouse cerebral cortex and spinal cord after being transplanted into adult rat with single-cell suspension by micro-transplantation method.
     本研究的目的是 :将体外培养的小鼠胚胎大脑皮层和脊髓的神经干细胞经单细胞悬液微移植后观察其在大鼠纹状体的存活、迁移和分化的状况。
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     The cultured neural stem cells in serum-free medium from embryo mouse cortex and spinal cord were expanded in vitro and labeled by DiI, and then labeled stem cells were transplanted into the adult rat brain striatum by micro-transplantation method as single-cell suspension with stereo-injection technique.
     实验在无血清条件下将这些细胞扩增、培养再经活细胞荧光染料 Di I标记后 ,采用微移植的方法 ,通过脑立体定位仪上用微玻璃针将干细胞分别植入成年大鼠双侧纹状体的对称部位。
短句来源
     The results showed that neural stem cells from embryo mouse cortex and spinal cord which were expanded in vitro could survive, migrate into the adult rat striatum and were distributed along special striatum structure after being transplanted by micro-transplantation method, but most of them differentiated into glia cells.
     结果表明 :来源于胚胎小鼠大脑皮层和脊髓的体外培养神经干细胞经微移植后 ,均可在成年大鼠脑内纹状体区域存活 ,移植的神经干细胞可向周围的脑实质内迁移 ,迁移细胞沿特定的纹状体结构分布。 神经干细胞主要分化成星形胶质细胞。
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  “微移植”译为未确定词的双语例句
     BDNF and NT-3 expression in transplantation cells were detected immunofluorescence assay.
     Hoechest33342标记后微移植方法将其移入PD大鼠模型纹状体内,免疫荧光技术检测移植细胞表达BDNF、NT-3状况。
短句来源
     A study on instrastriatal microtransplantation of cultured embryonic rat nigral cells suspension in rat hemiparkinsonism
     大鼠胎脑黑质细胞培养及PD鼠纹状体内悬液微移植研究
短句来源
     Group A was sham-graft group. Group B received non-cultured fresh nigral cells single-site macro-graft. Group C received non-cultured mutiple-sites micro-graft.
     A组为假手术组 ,B组为立即单靶点移植组 ,C组为立即多靶点微移植组 ,D组为培养后多靶点微移植组。
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     2.Multiple-sites microtransplantation can enhace the survival rate and the degree of reinnervation,decrease non-specific inflammatory or glial cell reaction.
     2多靶点微移植较经典悬液移植技术能提高存活率和神经纤维再分布程度 ,减轻非特异性炎症和胶质细胞反应。
短句来源
     Results NSCs could survive, migrate and incorporate into the host brain tissue and were distributed along special striatum structure, but a few of them differentiated into neurons.
     结果菲立磁标记的兔骨髓基质源神经干细胞经微移植后可在兔脑内纹状体区域存活,移植的干细胞可向周围的脑实质内迁移和整合,迁移细胞沿特定的纹状体结构分布。
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  相似匹配句对
     Treatment of baldness with hair-bearing scalp minigrafting
     头皮条移植治疗秃发
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     Heterotopic transplantation of autogenous minute granular bone
     自体小颗粒骨异位移植的实验研究
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     Immunology on Transplantation
     移植免疫
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     Micro-accelerometer
     加速度计
     Nondifferentiable D.
     不可D.
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Objective:How to enchance survival rate and preservate donor tissue is becoming promising research items.Methods:We caried out primary culture 6 days to embryonic nigral cells,indentify its biological character with immunohistochemical method.The different effects of different graft methods were compared by post-grafted survival rate,glial cell reaction and function recovery.Experimental rats were devided into four groups(A,B,C,D).Group A was sham-graft group.Group B received non-cultured fresh nigral cells...

Objective:How to enchance survival rate and preservate donor tissue is becoming promising research items.Methods:We caried out primary culture 6 days to embryonic nigral cells,indentify its biological character with immunohistochemical method.The different effects of different graft methods were compared by post-grafted survival rate,glial cell reaction and function recovery.Experimental rats were devided into four groups(A,B,C,D).Group A was sham-graft group.Group B received non-cultured fresh nigral cells single-site macro-graft.Group C received non-cultured mutiple-sites micro-graft.Group D received cultured mutiple-sitesmicro-graft.Results:At post-graft,group B、C、D demonstrated behavior functional recovery.Variation of group C was much bigger than that of group B(P<0.01).The alteration of group D was very significant than that of group C at post-grafted 1、2month (P<0.01).Histopathology data demonstrated that group C、D had higher cells survival rate and optimal graft integration and minimal disturbances of host brain structure,minimal inflamatory of glial cells reaction can reliably achieve.The back-fiow of cell suspension was aliminated.Moreover,we observed the developed pattern of survival cell in host brain,that was survive-migrate-differentate-integrate-reinnervate.Conclusion:1.The grafted nigral cells can reconstruct the nervous function though integrating into host brain.2.Multiple-sites microtransplantation can enhace the survival rate and the degree of reinnervation,decrease non-specific inflammatory or glial cell reaction.3.The primary culture of nigral cell afford experimental evidence for indentifing its biotic character in vitro and making the bank of donor brain tissne.4.We must pay attention to the lesson from these experimental course.

目的 探讨如何提高移植存活率和保存供体组织。方法 :胎脑黑质细胞原代培养 6天后 ,做免疫组化染色鉴定其生物活性 ,进行移植质量的人为控制。然后 ,比较各种移植技术对移植后细胞存活率、胶质细胞反应程度及功能改善的影响。实验动物分 A、B、C、D四组。A组为假手术组 ,B组为立即单靶点移植组 ,C组为立即多靶点微移植组 ,D组为培养后多靶点微移植组。结果 :B、C、D组术后均有功能改善 ,C组各时间点均比 B组改善显著 (P<0 .0 1) ,D组术后 1月、2月的功能改善程度较 C组明显 P<0 .0 1)。组织学也证实 :C、D组与 B组相比 ,移植细胞存活率及与宿主脑的整合程度显著提高 ,细胞外溢现象少 ,炎症及胶质细胞反应轻微 ,且移植细胞有存活—迁移—分化—神经纤维再分布的发育规律。结论 :1移植的黑质细胞能通过结构整合重塑神经功能。2多靶点微移植较经典悬液移植技术能提高存活率和神经纤维再分布程度 ,减轻非特异性炎症和胶质细胞反应。 3黑质细胞体外培养为其生物活性鉴定和建立供体组织库提供实验依据。 4移植实验过程中的几点体会值得注意。

The purpose of the present study was to investigate the survival, migration and differentiation of neural stem cells which were isolated and expanded in vitro from mouse cerebral cortex and spinal cord after being transplanted into adult rat with single-cell suspension by micro-transplantation method. The cultured neural stem cells in serum-free medium from embryo mouse cortex and spinal cord were expanded in vitro and labeled by DiI, and then labeled stem cells were transplanted into the adult rat brain striatum...

The purpose of the present study was to investigate the survival, migration and differentiation of neural stem cells which were isolated and expanded in vitro from mouse cerebral cortex and spinal cord after being transplanted into adult rat with single-cell suspension by micro-transplantation method. The cultured neural stem cells in serum-free medium from embryo mouse cortex and spinal cord were expanded in vitro and labeled by DiI, and then labeled stem cells were transplanted into the adult rat brain striatum by micro-transplantation method as single-cell suspension with stereo-injection technique. After 8 w, the operated rats were fixed, and cryostat slice were obtained and the morphology of the transplanted stem cells and their migration were observed under fluorescence microscope. Astrocyte specific GFAP was detected by immunocytochemistry to show the differentiation fate of the transplanted stem cells. The results showed that neural stem cells from embryo mouse cortex and spinal cord which were expanded in vitro could survive, migrate into the adult rat striatum and were distributed along special striatum structure after being transplanted by micro-transplantation method, but most of them differentiated into glia cells. The results indicated that the transplanted neural stem cells can survive, migrate and differentiate in the rat striatum.

本研究的目的是 :将体外培养的小鼠胚胎大脑皮层和脊髓的神经干细胞经单细胞悬液微移植后观察其在大鼠纹状体的存活、迁移和分化的状况。实验在无血清条件下将这些细胞扩增、培养再经活细胞荧光染料 Di I标记后 ,采用微移植的方法 ,通过脑立体定位仪上用微玻璃针将干细胞分别植入成年大鼠双侧纹状体的对称部位。大鼠存活 8周后 ,经灌注固定、恒冷箱切片 ,在荧光显微镜下观察标记的移植细胞的迁移状况 ;用星形胶质细胞特异性抗体观察移植区 GFAP的表达 ,以显示移植细胞分化状况。结果表明 :来源于胚胎小鼠大脑皮层和脊髓的体外培养神经干细胞经微移植后 ,均可在成年大鼠脑内纹状体区域存活 ,移植的神经干细胞可向周围的脑实质内迁移 ,迁移细胞沿特定的纹状体结构分布。神经干细胞主要分化成星形胶质细胞。本实验结果提示 ,移植的神经干细胞可在脑实质内存活、迁移和分化。

Objective To investigate the survival, migration, differentiation and incorporation of Feridex-labeled bone marrow stromal cells derived neural stem cells(BMSCs-D-NSCs) after being transplanted into rabbit striatum with single cell suspension by autologous microtransplantation method and to investigate the possibility of a new cell source for central nervous system transplantation. Methods BMSCs were acquired from the marrow of rabbit thighbone. NSCs culture medium, bFGF and EGF were used to induce...

Objective To investigate the survival, migration, differentiation and incorporation of Feridex-labeled bone marrow stromal cells derived neural stem cells(BMSCs-D-NSCs) after being transplanted into rabbit striatum with single cell suspension by autologous microtransplantation method and to investigate the possibility of a new cell source for central nervous system transplantation. Methods BMSCs were acquired from the marrow of rabbit thighbone. NSCs culture medium, bFGF and EGF were used to induce BMSCs proliferation and differentiation into BMSCs-D-NSCs in vitro. After these cells were further labeled by Feridex and PKH67, they were transplanted into rabbit striatum by microtransplantation method as single cell suspension with stereoinjection technique. After 1, 4, and 8 weeks, rabbits were sacrificed and their brain tissues were sectioned to investigate the morphology of the transplanted cells under microscopy and electromicroscopy. Results NSCs could survive, migrate and incorporate into the host brain tissue and were distributed along special striatum structure, but a few of them differentiated into neurons. Conclusion The transplanted neural stem cells can survive, migrate, differentiate and incorporate in the rabbit striatum. BMSCs may be a potential source for cell transplantation in treating CNS discorders in humans.

目的将体外标记的骨髓基质源神经干细胞经单细胞悬液微移植后观察其在兔纹状体的存活、迁移、分化和整合情况,为细胞移植治疗疾病奠定基础。方法分离兔骨髓基质细胞,利用神经干细胞培养基、白血病抑止因子和碱性成纤维母细胞生长因子进行细胞扩增并诱导成骨髓基质源神经干细胞,再经菲立磁和活细胞荧光染料PKH67标记后,采用微移植的方法,通过脑立体定位仪,用微玻璃针将干细胞分别植入兔脑纹状体内。存活1、4、8周后处死动物,组织切片,利用光镜和电镜观察标记细胞在脑内的形态学情况。结果菲立磁标记的兔骨髓基质源神经干细胞经微移植后可在兔脑内纹状体区域存活,移植的干细胞可向周围的脑实质内迁移和整合,迁移细胞沿特定的纹状体结构分布。少量菲立磁标记的干细胞可以分化成神经元。结论骨髓基质源神经干细胞移植后,可在脑实质内存活、迁移、分化和整合,这种细胞可能成为中枢神经系统自体移植的细胞来源。

 
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