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irna
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  i-rna
     STUDY ON TRANSFER OF HUMORAL IMMUNAL ACTIVITY BY I-RNA AND ITS EFFECT ON BODY IMMUNITY
     抗菌i-RNA没有传递特异性体液免疫活性能力的确定及其对机体免疫功能影响的研究
短句来源
     With the method of MTT colorimetery,the effect of immune RNA(i-RNA )oninterleukin-2(IL-2)activity of newborn infants born to mothers with cytomegalovirus(CMV)infection was tested.
     本研究将特异性免疫核糖核酸(i-RNA)用于巨细胞病毒(CMV)感染母亲所生的婴儿,采用四甲基偶氮唑盐(MTT)比色法,测定了治疗前后白细胞介素2(IL-2)的活性。
短句来源
     This study providestheoretical basis for the use of i-RNA to prevent CMV vertical infection from mother-toinfants.
     本研究为临床应用特异性i-RNA预防CMV母婴垂直感染提供了实验依据。
短句来源
     In this paper was studied the possible effect of immune RNA (i-RNA) on activity of natural killer (NK) cells and plasma interferon (IFN ) titer of newborn infants born from mothers with positive IgM antibody to cytomegalovirus (CMV) by use of lactic dehydrogenase (LDH) release assay and microcytopathic inhibit assay.
     本研究采用乳酸脱氢酶(LDH)释放法和微量细胞病变抑制法,观察了i-RNA对CMV感染母亲所生婴儿外周血自然杀伤(NK)细胞活性和血浆干扰素(IFN)水平的影响。
短句来源
     The above data indicated that i-RNA can enhance activity of NK cells and plasma IFN titer of newborn infants. This study provided theoretical basis for prevention of CMV vertical infection in mother-infants with i-RNA
     证明抗CMV的i-RNA对新生儿外周血NK细胞活性和血浆IFN水平均具有明显的增强作用。
短句来源
  “i-rna”译为未确定词的双语例句
     Therewas marked difference in IL-2 activity in the two groups(P<0.01).
     两组比较差异有显著性(P<0.01),提示特异性i-RNA对新生儿外周血IL-2活性有明显的增强作用。
短句来源
     Conclusion: Antiviral i RNA can enhance the humoral immunity and cellular immunity of the host.
     结论:抗病毒i-RNA可增强宿主体液免疫和细胞免疫。
短句来源
     demonstrated that antibody productivity, engulfmentactivity of macroghages, NK activity of spleen cells and IL-2 activityin iRNA-immunized groups were all significantly higher than that innormal group. So it can be proyed that Pa-iRNA has adjuvant activity,and it can enhance humoral immunity of the organism and immunity ofthe cells.
     但小鼠体内脾细胞抗体产生能力,巨噬细胞吞噬活性,脾细胞NK活性和IL-2活性均明显高于正常鼠,提示抗菌i-RNA有佐剂活性,可以增强机体的体液免疫功能和细胞免疫功能。
短句来源
  相似匹配句对
     RNA of changing with each passing day
     日新月异的RNA
短句来源
     RNA Splicing
     RNA的拼接
短句来源
     The Principle and Application of AmpliDet RNA
     AmpliDet RNA技术
短句来源
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  irna
Nine formations are described: Um Irna (80 m), Ma'in (40 m), Dardun (60 m), Ain Musa (80 m), Hisban (35 m), Mukheiris (90 m), Iraq al Amir (170 m), Um Tina (260 m), Abu Ruweis (200 m).
      
A low molecular weight RNA species, in the 70-90 nucleotide size range (iRNA), has been purified from the ribosomal salt wash of chick embryonic muscle by a combination of DEAE-cellulose and hydroxyapatite chromatography.
      
This method yields iRNA free from contaminating tRNA and gives better and more reproducible yields than those obtained with our previous method involving lengthy dialysis of the salt wash.
      
The iRNA at a concentration of 20-80 ng range strongly inhibits the translation of homologous and heterologous mRNAs i.e.
      
chick muscle poly(A)+mRNA and rabbit globin mRNA; uncapped mRNA; and poly(A)-mRNA in micrococcal nuclease-treated reticulocyte lysate indicating that inhibition by iRNA is nonselective in nature.
      
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For the simultaneous extraction of transfer factor and immune RNA (iRNA) from human leucocytes the second part is described in this paper. The iRNA was extracted from the residue of the leucocyte homogenate by a modified method of Scherrer and Darnell using hot phenol with sodium dodecyl sulfate. One to two mg of iRNA was obtained per gram of leucocytes. The physical and chemical properties of iRNA were analysed: A_(260)/A_(230)=2.05~2.26, A_(260)/A_(280)=2.03~2.20. The preparation contained...

For the simultaneous extraction of transfer factor and immune RNA (iRNA) from human leucocytes the second part is described in this paper. The iRNA was extracted from the residue of the leucocyte homogenate by a modified method of Scherrer and Darnell using hot phenol with sodium dodecyl sulfate. One to two mg of iRNA was obtained per gram of leucocytes. The physical and chemical properties of iRNA were analysed: A_(260)/A_(230)=2.05~2.26, A_(260)/A_(280)=2.03~2.20. The preparation contained RNA 48.33±4.72%; DNA 4.31±2.53%, protein 2.76± 0.32%, and glycogen 42.92±9.92%. Five bands were revealed in polyacrylamide gel electrophoresis corresponding to 4S, 8S, 9S, 15S and 20S RNA's.Animal experiments demonstrated that the lyophilised preparation was nontoxic, non-pyrogenic and non-anaphylactic, but was antigenic in rabbits. The antigenicity remained after treatment with RNase, DNase or pronase. The antigenicity, however, was lost when the preparation was treated with salivary amylase. The antigenicity of the preparation was probably due to glycogen.The preparation dissolved in heparin solution was used on various patients. The delayed-type skin hypersensitivity reaction and cell-mediated immunity of the patients were usually augmented.

本文继续介绍联合抽提法的第Ⅱ部分“免疫”核糖核酸(iRNA)的抽提。将白细胞匀浆沉淀残渣按Scherrer和Darnell的十二烷基磺酸钠加苯酚的热抽提法抽提。每克白细胞可得iRNA1~2毫克。A_(260)/A_(230)=2.05~2.26,A_(260)/A_(280)=2.03~2.20。RNA含量为48.33±4.72%,DNA含量为4.31±2.53%,蛋白质为2.76±0.32%,糖原含量为42.92±9.92%。聚丙烯酰胺凝胶电泳显示五条区带,相当于4S、83、9S、15S和20S。冻干的iRNA制剂经动物实验证明无毒性、无热原和无过敏性,但对家兔具有抗原性。制剂经RNase、DNase或pronase处理后与抗血清仍有沉淀反应,但经唾液淀粉酶处理后沉淀线消失。所以认为抗原性系由制剂中所含糖原所引起。溶于肝素溶液的制剂注射于病人,能提高其迟发型皮肤变态反应及细胞免疫反应。

For the simultaneous extraction of transfer factor and immune RNA(iRNA)from human leucocytes the second part is described in this paper.The iRNA wasextraoted from the residue of the leuoocyte homogenate by a modified method ofScherrer and Darnell using hot phenol with sodium dodecyl sulfate.One to two mg ofiRNA was obtained per gram of leucocytes.The physical and chemical propertiesof iRNA were analysed:A_(260)/A_(230)=2.05~2.26,A_(260)/A_(280)=2.03~2.20.Thepreparation oontained RNA 48.33±4.72%;DNA...

For the simultaneous extraction of transfer factor and immune RNA(iRNA)from human leucocytes the second part is described in this paper.The iRNA wasextraoted from the residue of the leuoocyte homogenate by a modified method ofScherrer and Darnell using hot phenol with sodium dodecyl sulfate.One to two mg ofiRNA was obtained per gram of leucocytes.The physical and chemical propertiesof iRNA were analysed:A_(260)/A_(230)=2.05~2.26,A_(260)/A_(280)=2.03~2.20.Thepreparation oontained RNA 48.33±4.72%;DNA 4.31±2.53%,protein 2.76±0.32%,and glycogen 42.92±9.92%.Five bands were revealed in polyacrylamide gelelectrophoresis oorresponding to 4S,8S,9S,15S and 20S RNA's.Animal experiments demonstrated that the lyophilised preparation was nontoxic,non-pyrogenic and non-anaphylactic,but was antigenic in rabbits.The antigenicityremained after treatment with RNase,DNase or pronase.The antigenicity,however,was lost when the preparation was treated with salivary amylase.The antigenicity ofthe preparation was probably due to glycogen.The preparation dissolved in heparin solution was used on various patients.Thedelayed-type skin hypersensitivity reaction and cell-mediated immunity of the patientswere usually augmented.

本文继续介绍联合抽提法的第Ⅱ部分“免疫”核糖核酸(iRNA)的抽提。将白细胞匀浆沉淀残渣按Scherrer 和Darnell 的十二烷基磺酸钠加苯酚的热抽提法抽提。每克白细胞可得iRNAl~2毫克。A_(260)/A_(230)=2.05~2.26,A_(260)/A_(280)=2.03~2.20。RNA 含量为48.33±4.72%,DNA 含量为4.31±2.53%,蛋白质为2.76±0.32%,糖原含量为42.92±9.92%。聚丙烯酰胺凝胶电泳显示五条区带,相当于4S、8S、9S、15S 和20S。冻干的iRNA 制剂经动物实验证明无毒性、无热原和无过敏性,但对家兔具有抗原性。制剂经RNase、DNase 或pronase 处理后与抗血清仍有沉淀反应,但经唾液淀粉酶处理后沉淀线消失。所以认为抗原性系由制剂中所含糖原所引起。溶于肝素溶液的制剂注射于病人,能提高其迟发型皮肤变态反应及细胞免疫反应。

When rabbit lymphocytes were treated with HBsAg-iRNA, isolated from sheep immunized with HBsAg, and were incubated with HBsAg, the incorporation of 3HdT into lymphocytes treated was twice as that of the control, but the lymphocytes treated with Freund's complete adjuvant immune RNA (n-RNA) were the same as the control. When the HBsAg-iRNA was digested by RNase, the increase of 3HdT incorporation into lymphocytes treated with HBsAg-iRNA disappeared. Whea lymphocytes were only treated with HBsAg-iRNA...

When rabbit lymphocytes were treated with HBsAg-iRNA, isolated from sheep immunized with HBsAg, and were incubated with HBsAg, the incorporation of 3HdT into lymphocytes treated was twice as that of the control, but the lymphocytes treated with Freund's complete adjuvant immune RNA (n-RNA) were the same as the control. When the HBsAg-iRNA was digested by RNase, the increase of 3HdT incorporation into lymphocytes treated with HBsAg-iRNA disappeared. Whea lymphocytes were only treated with HBsAg-iRNA but incubated without HBsAg, or only incubated with HBsAg but not treated with iRNA before, their incorporation of 3HdT was not higher, as compared with the corresponding controls. The above results showed that HBsAg-iRNA could transfer cellular immune reactivity of specific lymphocytio transformation against HBsAg beyond species limit, causing the increase of 3HdT incorporation into lymphocytes.

本文报道用绵羊中提取的乙型肝炎表面抗原-免疫核糖核酸(HBsAg-iRNA)处理的正常兔血细胞,在加HBsAg培养时,其淋巴细胞参入氚化脱氧胸苷(~3HdT)的量为对照管的2倍,而用佐剂免疫动物提取的核糖核酸(n-RNA)则否。将HBsAg-iRNA用核糖核酸酶(RNase)消化后,则促~3HdT参入作用即消失。如单独用HBsAg-iRNA处理淋巴细胞而不加HBsAg培养,或不用HBsAg-iRNA处理而加HBsAg培养,均不能使~3HdT的参入增加。以上结果表明,HBsAg-iRNA能超越种间界限,传递给淋巴细胞,使其在接触HBsAg时发生特异转化作用,增加~3HdT参入而显示其免疫反应性。

 
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